Article
Cell Biology
Chen Liu, Sujuan Lei, Tianying Cai, Yonglang Cheng, Junjie Bai, Wenguang Fu, Meizhou Huang
Summary: This study elucidates the role of iNOS in vascular endothelial dysfunction through cellular experiments and metabolic analysis. The findings suggest that iNOS deficiency can reverse the pathological changes induced by TNF-α and alleviate vascular endothelial dysfunction.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Environmental Sciences
Dalia H. El-Kashef, Maha H. Sharawy
Summary: Nicorandil protects against acetaminophen-induced liver damage by reducing liver function indicators, inhibiting oxidative stress and inflammation, and improving cell survival.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Article
Biochemistry & Molecular Biology
Robert J. Webber, Richard M. Sweet, Douglas S. Webber
Summary: The study found that anti-MV-A iNOS monoclonal antibodies can mitigate the harmful effects of MV-A iNOS and increase the survival rate of mice challenged with sepsis. The efficacy of the treatment is both time- and dose-dependent. Humanized anti-MV-A iNOS mAbs could potentially save tens of thousands of lives annually and improve antibiotic stewardship.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mireia Lopez-Riera, Rebeca Ortega, Luisa Hueso, Maria Carmen Montesinos, Mari Carmen Gomez-Cabrera, Maria Jesus Sanz, Jose T. Real, Laura Piqueras
Summary: This study demonstrates that CAR agonists can inhibit the early inflammatory response that precedes atherosclerosis by targeting various steps in the leukocyte recruitment cascade. This suggests that CAR agonists may serve as a new therapeutic approach to control inflammation associated with cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Annett Eitner, Sylvia Mueller, Christian Koenig, Arne Wilharm, Rebecca Raab, Gunther O. Hofmann, Thomas Kamradt, Hans-Georg Schaible
Summary: IL-1 beta induces NO release and mitochondrial dysfunction, and both can be prevented by inhibiting iNOS with L-NIL. Additionally, enhancing cAMP or inhibiting AMPK can reduce the effects of IL-1 beta, preventing NO release and mitochondrial dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Layal El-Hajjar, Judy Hindieh, Rana Andraos, Marwan El-Sabban, Jalil Daher
Summary: Cardiovascular disease caused by atherosclerosis is a major cause of death worldwide. This study explores the role of oxidized LDL and the LOX-1 scavenger receptor in endothelial dysfunction and inflammation, providing insight into the pathways involved in atherosclerosis progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cardiac & Cardiovascular Systems
Hui Jiang, Yongwen Zhou, Seyed M. Nabavi, Amirhossein Sahebkar, Peter J. Little, Suowen Xu, Jianping Weng, Jianjun Ge
Summary: Atherosclerosis is an immuno-metabolic disease involving chronic inflammation, oxidative stress, epigenetics, and metabolic dysfunction. Oxidation of low-density lipoprotein (LDL) is a primary modification in atherosclerosis, leading to pathophysiological changes and plaque formation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Philipp Jordan, Amanda Costa, Edgar Specker, Oliver Popp, Andrea Volkamer, Regina Piske, Tessa Obrusnik, Sabrina Kleissle, Kevin Stuke, Andre Rex, Martin Neuenschwander, Jens Peter von Kries, Marc Nazare, Phillip Mertins, Helmut Kettenmann, Susanne A. Wolf
Summary: Microglia, as the immune effector cells of the CNS, respond to pathologic events by releasing nitric oxide (NO). A compound called C1 was found to significantly reduce NO release in a dose-dependent manner, with a low IC50 (252 nM), and no toxic effects in vitro or in vivo. Target finding strategies, such as in silico modelling and mass spectroscopy, suggest a direct interaction between C1 and nitric oxide synthase, making C1 a promising candidate for specific intra-cellular interaction with the NO producing machinery.
Review
Biochemistry & Molecular Biology
Hasnae Boughaleb, Irina Lobysheva, Flavia Dei Zotti, Jean-Luc Balligand, Virginie Montiel
Summary: This review focuses on a novel technique of directly measuring HbNO and discusses its correlation with vascular endothelial function as well as its potential applications in disease monitoring and prediction.
Article
Geriatrics & Gerontology
Huilin Yu, Ke Liao, Yu Hu, Dingyi Lv, Minghao Luo, Qian Liu, Longxiang Huang, Suxin Luo
Summary: This study investigates the role of the cGAS-STING pathway in aging-related endothelial dysfunction, demonstrating its potential as a target for the prevention of cardiovascular diseases in the elderly.
Review
Physiology
Karim Ullah, Rongxue Wu
Summary: Endothelial cells play a crucial role in maintaining cardiovascular homeostasis, tissue growth, and repair, relying on glycolysis to resist hypoxic damage and promote angiogenesis in ischemic regions. Hypoxia-inducible factors (HIFs) are key regulators of endothelial cell metabolism and angiogenesis, with varying effects on cardiovascular dysfunction in different disease settings.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Annapoorna Mohandas, Jayakumar Rangasamy
Summary: This study developed an injectable nanocurcumin and arginine incorporated chitosan hydrogel to prevent hypoxia induced endothelial damage. The hydrogel showed significant antioxidant activity and biodegradation in vitro, with better release of curcumin and arginine at acidic pH. The hydrogel effectively reduced the effect of hypoxia induced endothelial dysfunction in cultured endothelial cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Medicine, Research & Experimental
Jun -Ha Hwang, Woong Heo, Jung Il Park, Kyung Min Kim, Ho Taek Oh, Gi Don Yoo, Jeekeon Park, Somin Shin, Youjin Do, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong
Summary: Endothelial TAZ is involved in the production of nitric oxide (NO) and inhibits damage-induced liver fibrosis. It regulates NO production by transcriptional regulation of Nos3. Loss of TAZ leads to capillarization of liver sinusoidal endothelial cells and a decrease in NO concentration, accelerating liver fibrosis.
Article
Cell Biology
Dingyi Lv, Minghao Luo, Jianghong Yan, Xiyang Yang, Suxin Luo
Summary: This study demonstrates the critical role of SIRT3 in endothelial dysfunction in sepsis, with protective effects against vascular inflammation by inhibiting the activation of NF-kappa B and NLRP3 pathways, and maintaining the expression of endothelial nitric oxide synthase and endothelium-dependent relaxation function. These findings suggest a potential therapeutic target in vascular dysfunction and septic shock.
Article
Plant Sciences
Vincenzo Musolino, Roberta Macri, Antonio Cardamone, Maria Serra, Anna Rita Coppoletta, Luigi Tucci, Jessica Maiuolo, Carmine Lupia, Federica Scarano, Cristina Carresi, Saverio Nucera, Irene Bava, Mariangela Marrelli, Ernesto Palma, Micaela Gliozzi, Vincenzo Mollace
Summary: This study used a polystyrene resin to recover polyphenolic compounds from the leaf extract of Nocellara del Belice cultivar and evaluated their antioxidant and anti-inflammatory activities. The extract showed good radical scavenging capability and was able to reduce intracellular lipid accumulation.
Review
Biology
Jessica Maiuolo, Rosa Maria Bulotta, Francesca Oppedisano, Francesca Bosco, Federica Scarano, Saverio Nucera, Lorenza Guarnieri, Stefano Ruga, Roberta Macri, Rosamaria Caminiti, Vincenzo Musolino, Micaela Gliozzi, Cristina Carresi, Antonio Cardamone, Annarita Coppoletta, Martina Nicita, Adriano Carnevali, Vincenzo Scorcia, Vincenzo Mollace
Summary: Eye health is crucial and diseases can greatly impact vision and quality of life. Natural antioxidants and nutraceutical supplementation can counteract oxidative stress in early stages of eye diseases and improve vision. Combining nutraceuticals and antioxidants may be a promising solution for eye protection.
Review
Agronomy
Jessica Maiuolo, Rocco Mollace, Francesca Bosco, Federica Scarano, Francesca Oppedisano, Saverio Nucera, Stefano Ruga, Lorenza Guarnieri, Roberta Macri, Irene Bava, Cristina Carresi, Micaela Gliozzi, Vincenzo Musolino, Antonio Cardamone, Anna Rita Coppoletta, Andrea Barillaro, Virginia Simari, Daniela Salvemini, Ernesto Palma, Vincenzo Mollace
Summary: Non-alcoholic fatty liver disease (NAFLD) is a leading cause of liver-related health issues. There is currently no unique drug treatment for NAFLD, and natural products such as bergamot polyphenolic fraction (BPF) and Cynara cardunculus extract have shown potential in counteracting the disease's effects. These extracts have been shown to inhibit lipid accumulation, oxidative stress, inflammation, and fibrosis in liver cells, leading to improved cardiac and endothelial function. Supplementation with BPF and Cynara cardunculus extract may offer a novel approach in preventing and treating NAFLD-related complications.
Article
Biochemistry & Molecular Biology
Francesca Bosco, Lorenza Guarnieri, Saverio Nucera, Miriam Scicchitano, Stefano Ruga, Antonio Cardamone, Samantha Maurotti, Cristina Russo, Anna Rita Coppoletta, Roberta Macri, Irene Bava, Federica Scarano, Fabio Castagna, Maria Serra, Rosamaria Caminiti, Jessica Maiuolo, Francesca Oppedisano, Sara Ilari, Filomena Lauro, Luigi Giancotti, Carolina Muscoli, Cristina Carresi, Ernesto Palma, Micaela Gliozzi, Vincenzo Musolino, Vincenzo Mollace
Summary: The aim of this study was to evaluate if chronic constriction injury (CCI) of the sciatic nerve in rats can be a valid model to study muscle atrophy and consequent osteoporosis. The results showed that CCI can cause significant decreases in muscle mass and bone mass and alterations in the expression of molecular markers of muscle atrophy and osteoporosis. This study suggests that CCI may serve as a valid approach to study muscle-bone crosstalk and to identify new strategies to prevent osteosarcopenia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Jessica Maiuolo, Natalizia Miceli, Federica Davi, Irene Bava, Luigi Tucci, Salvatore Ragusa, Maria Fernanda Taviano, Vincenzo Musolino, Micaela Gliozzi, Cristina Carresi, Roberta Macri, Federica Scarano, Anna Rita Coppoletta, Antonio Cardamone, Carolina Muscoli, Ezio Bombardelli, Ernesto Palma, Vincenzo Mollace
Summary: Ferula L. plant extract has therapeutic effects on breast cancer and can be used in combination with tamoxifen to enhance its effectiveness and reduce side effects.
Review
Biochemistry & Molecular Biology
Cinzia Garofalo, Costanza Maria Cristiani, Sara Ilari, Lucia Carmela Passacatini, Valentina Malafoglia, Giuseppe Viglietto, Jessica Maiuolo, Francesca Oppedisano, Ernesto Palma, Carlo Tomino, William Raffaeli, Vincenzo Mollace, Carolina Muscoli
Summary: Fibromyalgia and Irritable Bowel Syndrome share similar symptoms and may have a common etiology related to alterations in gut microbiota. This review will discuss the similarities between FM and IBS and the potential therapeutic approaches based on microbiota manipulations.
Review
Veterinary Sciences
Roberto Bava, Fabio Castagna, Ernesto Palma, Mariangela Marrelli, Filomena Conforti, Vincenzo Musolino, Cristina Carresi, Carmine Lupia, Carlotta Ceniti, Bruno Tilocca, Paola Roncada, Domenico Britti, Vincenzo Musella
Summary: The Varroa destructor parasite poses a major threat to honey bee colonies. While eco-friendly plant essential oils (EOs) have gained interest as potential solutions, there is inconsistency in research results due to different study techniques and variability in plant chemical compositions. This review aims to summarize the research on using EOs to control the parasite, standardize results, and suggest future research directions.
VETERINARY SCIENCES
(2023)
Review
Nutrition & Dietetics
Francesca Bosco, Stefano Ruga, Rita Citraro, Antonio Leo, Lorenza Guarnieri, Jessica Maiuolo, Francesca Oppedisano, Roberta Macri, Federica Scarano, Saverio Nucera, Irene Bava, Ernesto Palma, Carolina Muscoli, Juan Hancke, Giovambattista De Sarro, Vincenzo Mollace
Summary: Neurodegenerative diseases, such as Alzheimer's and Parkinson's, are common worldwide. Aging process is the most significant risk factor, but genetic and environmental factors also play a role. Lifestyle and natural products containing antioxidant and anti-inflammatory properties can have preventive and therapeutic effects. Andrographis paniculata and its main constituent, andrographolide, have shown neuroprotective effects by modulating neurodegeneration and neuroinflammation.
Review
Food Science & Technology
Carlotta Ceniti, Anna Antonella Spina, Cristian Piras, Francesca Oppedisano, Bruno Tilocca, Paola Roncada, Domenico Britti, Valeria Maria Morittu
Summary: To ensure the safety and quality of milk, new tools have been developed to detect chemical contaminants, toxins, veterinary drugs, and adulteration of milk from different species. Mid-infrared spectroscopy and Fourier-transform infrared have been commonly used technologies for rapid screening of hazardous substances and confirmation of milk authenticity. These fingerprint methods can effectively determine the characteristics of raw materials without knowing the identity of each constituent, making them potential screening methods for adulteration detection. This paper reviews the latest advances in applying mid-infrared spectroscopy for the detection and quantification of adulterants, milk dilution, pathogenic bacteria, veterinary drugs, and hazardous substances in milk.
Article
Plant Sciences
Vincenzo Musolino, Roberta Macri, Antonio Cardamone, Luigi Tucci, Maria Serra, Carmine Lupia, Samantha Maurotti, Rosario Mare, Saverio Nucera, Lorenza Guarnieri, Mariangela Marrelli, Anna Rita Coppoletta, Cristina Carresi, Micaela Gliozzi, Vincenzo Mollace
Summary: This study extracted terpenoids-rich and polyphenols-rich rosemary extracts through maceration and ultrasound sonication, and investigated their phytochemical characteristics, antioxidant activity, and potential benefits in reducing lipid accumulation in NAFLD.
Article
Biochemistry & Molecular Biology
Roberta Macri, Irene Bava, Federica Scarano, Rocco Mollace, Vincenzo Musolino, Micaela Gliozzi, Marta Greco, Daniela Foti, Luigi Tucci, Jessica Maiuolo, Cristina Carresi, Annamaria Tavernese, Ernesto Palma, Carolina Muscoli, Vincenzo Mollace
Summary: This study aimed to test the effects of Ferutinin rich-Ferula communis L. root extract (FcFE) at different concentrations on H9C2 cells and evaluate its antioxidant properties in cardiomyocytes to explore new potential therapeutic activities. The results showed that FcFE could protect against Doxorubicin-induced cardiotoxicity. Further molecular characterization of this natural compound may open the way for testing FcFE at low concentrations in vivo and in clinical studies as an adjuvant in cancer therapy to prevent side effects on heart cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rocco Mollace, Roberta Macri, Martina Nicita, Vincenzo Musolino, Micaela Gliozzi, Cristina Carresi, Irene Bava, Jessica Maiuolo, Annamaria Tavernese, Antonio Cardamone, Luigi Tucci, Giuseppe Trunfio, Elzbieta Janda, Ernesto Palma, Carolina Muscoli, Francesco Barilla, Massimo Federici, Federica Scarano, Vincenzo Mollace
Summary: Evidence suggests that the gut microbiota plays a role in the alterations of lipid metabolism associated with high-fat diet. Recent evidence indicates that supplementation with natural polyphenols and fibres can improve high-fat diet-induced dysbiosis and improve the lipidaemic profile. This study aimed to investigate the protective effect of bergamot polyphenolic extract and fibres on high-fat diet-induced dyslipidaemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Rosita Stanzione, Maurizio Forte, Maria Cotugno, Francesca Oppedisano, Cristina Carresi, Simona Marchitti, Vincenzo Mollace, Massimo Volpe, Speranza Rubattu
Summary: High salt load is damaging to vascular cells and a risk factor for cardiovascular diseases. In this study, we tested the effects of a bergamot polyphenolic fraction (BPF) on high-salt-induced injury in cerebral endothelial cells. BPF reduced oxidative stress, improved cell viability and angiogenesis, and decreased mitochondrial oxidative stress. BPF may be a valuable adjunct for treating vascular disorders.