期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 130, 期 -, 页码 58-67出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.02.120
关键词
Hyaluronic acid; Silk fibroin; Sodium alginate; Scaffold; Skin repair
资金
- Natural Science Foundation of China, China [81573708, 31271019, 81171812, 81671924, 81272105]
- China National Key Research and Development Plan [2017YFC1103301]
- Natural Science Foundation of Guangdong Province, China [2015A030306049]
- Science and Technology Program of Guangzhou [201601010270, 2017010160489, 201704030083]
- Pearl River S&T Nova Program of Guangzhou, China [201710010155, 201806010072]
- Science and Technology Project of Guangdong Province, China [2015A010101313, 2017A050506011, 2017A050501013, 20178090911012, 2018A050506040, 2018A050506019, 2018A050506021]
To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying. Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules. Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93)11b. Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/ HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments. Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds. We evaluated the wound healing effects in a rat full-thickness burn model. The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling. Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair. (C) 2019 Published by Elsevier B.V.
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