期刊
INSECT MOLECULAR BIOLOGY
卷 29, 期 1, 页码 1-8出版社
WILEY
DOI: 10.1111/imb.12604
关键词
Culex quinquefasciatus; Wolbachia; cytoplasmic incompatibility; incompatible insect technique; population replacement; transinfection
资金
- Emerging and Major Infectious Diseases of Livestock (EMIDA) Eurpoean Research Area Networks (ERA Net) [219235]
- Wellcome Trust [108508, 202888]
- MRC [MC_UU_12014/8] Funding Source: UKRI
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population suppression caused by mating incompatibilities between mass-released transinfected males and wild females; and secondly, the spread of pathogen transmission-blocking strains through populations. Using embryonic microinjection, two novel Wolbachia transinfections were generated in C. quinquefasciatus using strains native to the mosquito Aedes albopictus: a wAlbB single infection, and a wPip plus wAlbA superinfection. The wAlbB infection showed full bidirectional cytoplasmic incompatibility (CI) with wild-type C. quinquefasciatus in reciprocal crosses. The wPipwAlbA superinfection showed complete unidirectional CI, and therefore population invasion potential. Whereas the wAlbB strain showed comparatively low overall densities, similar to the native wPip, the wPipwAlbA superinfection reached over 400-fold higher densities in the salivary glands compared to the native wPip, suggesting it may be a candidate for pathogen transmission blocking.
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