期刊
APPLIED PHYSICS LETTERS
卷 108, 期 20, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4950961
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资金
- DTU
We report results of studies relating to the fabrication of a flexible, disposable, and label free biosensing platform for detection of the cancer biomarker (carcinoembryonic antigen, CEA). Polyaniline (PANI) has been electrochemically deposited over gold sputtered paper (Au@paper) for covalent immobilization of monoclonal carcinoembryonic antibodies (anti-CEA). The bovine serum albumin (BSA) has been used for blocking nonspecific binding sites at the anti-CEA conjugated PANI/Au@Paper. The PANI/Au@Paper, anti-CEA/PANI/Au@Paper, and BSA/anti-CEA/PANI/Au@Paper platforms have been characterized using scanning electron microscopy, X-ray diffraction, Fourier transmission infrared spectroscopy, chronoamperometry, and electrochemical impedance techniques. The results of the electrochemical response studies indicate that this BSA/anti-CEA/PANI/Au@paper electrode has sensitivity of 13.9 mu A ng(-1) ml cm 2, shelf life of 22 days, and can be used to estimate CEA in the range of 2-20 ng ml(-1). This paper sensor has been validated by detection of CEA in serum samples of cancer patients via immunoassay technique. Published by AIP Publishing.
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