期刊
IMMUNITY
卷 51, 期 1, 页码 155-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2019.05.006
关键词
-
类别
资金
- National Institutes of Health [AI061061, CA216234, AI109962]
- National Cancer Institute
Genetic variation influences how the genome is interpreted in individuals and in mouse strains used to modelimmune responses. Wedevelopedapproaches to utilize next-generation sequencing datasets to identify sequence variation in genes and enhancer elements in congenic and backcross mouse models. We defined genetic variation in the widely used B6-CD45.2 and B6.SJL-CD45.1 congenic model, identifying substantial differences inSJL geneticcontent retained in B6.SJL-CD45.1 strains on the basis of the vendor source of the mice. Genes encoding PD-1, CD62L, Bcl-2, cathepsin E, and Cxcr4 were within SJL genetic content in at least one vendor source of B6.SJL-CD45.1 mice. SJL genetic content affected enhancer elements, gene regulation, protein expression, and amino acid content in CD4(+) T helper 1 cells, and mice infected with influenza showed reduced expression of Cxcr4 on B6.SJL-CD45.1 T follicular helper cells. These findings provide information on experimental variables and aid in creating approaches that account for genetic variables.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据