Article
Medicine, General & Internal
Yeonjeong Ha
Summary: Magnetic nanoparticles (MNPs) have significant potential in disease diagnostics, as they can enhance detection sensitivity, facilitate concentration and purification of target substances, and enable favorable color-based detection. This study successfully synthesized antibody-conjugated MNPs and validated them through size measurement and phosphatase methods. Additionally, three methods for developing color in MNPs-based immunoassay were suggested and implemented, and effective concentration and purification of target substances were demonstrated through the collection of MNPs using an external magnetic field.
Article
Immunology
Christelle Kassis, Michelle Durkin, Eric Holbrook, Robert Myers, Lawrence Wheat
Summary: Combining antibody and antigen detection can improve sensitivity and specificity in the diagnosis of coccidioidomycosis, particularly with higher sensitivity in immunocompromised patients. Antigen detection can help prevent missed diagnoses.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Chemistry, Physical
Deepa Sebastian, Munaifa Arif, Kala Ramakrishnan
Summary: A water soluble fluorescence immunoassay probe based on silicon quantum dot (SiQD) with aggregation induced emission features (AIE) was developed for the sensitive detection of C-reactive protein (CRP) biomarker.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Oncology
Giulia Pellizzari, Olivier Martinez, Silvia Crescioli, Robert Page, Ashley Di Meo, Silvia Mele, Giulia Chiaruttini, Jan Hoinka, Ihor Batruch, Ioannis Prassas, Melanie Grandits, Jacobo Lopez-Abente, Eva Bugallo-Blanco, Malcolm Ward, Heather J. Bax, Elise French, Anthony Cheung, Sara Lombardi, Mariangela Figini, Katie E. Lacy, Eleftherios P. Diamandis, Debra H. Josephs, James Spicer, Sophie Papa, Sophia N. Karagiannis
Summary: The study identified a novel tumor-specific target, SLC3A2, and successfully developed IgE monoclonal antibodies and SLC3A2-specific CAR T cells, which showed potent cytotoxicity against tumor cells in vitro and in vivo without triggering adverse reactions.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Multidisciplinary Sciences
Penghua Zhao, Xiaoyan Huang, Yaping Li, Xueping Huo, Qing Feng, Xiangrong Zhao, Cuixiang Xu, Jianhua Wang
Summary: This study developed a simple and efficient method to label proteins onto magnetic nanoparticles by synthesizing an artificial protein corona on the surface of nanoparticles. The optimized nanoparticles showed a narrow particle size distribution and improved biocompatibility. The results suggest that the artificial protein corona has promising potential for multifunctional biomedical applications.
Article
Chemistry, Analytical
Abiola Adesina, Omotayo Adeniyi, Philani Mashazi
Summary: Selective purification of biological materials for their detection in complex sample matrix is a general challenge in diagnostics. In this study, an ultrasensitive sandwich-based impedimetric immunosensor was developed for the detection of CRP antigen. The stable and oriented immobilization of anti-CRP monoclonal antibody on magnetic nanoparticles was achieved, and Electrochemical Impedance Spectroscopy was used for CRP detection.
Article
Chemistry, Multidisciplinary
Chaoqun Wang, Ziqiang Deng, Hu Zhang, Rui Liu, Yi Lv
Summary: Magnetic particles (MPs) are widely used in various biological applications, and this study discovered a novel function of commercialized MPs-internal standard for accurate quantification of biomolecules. The proposed MPs-based immunoassay greatly improves the accuracy and precision by eliminating potential MPs loss and intensity fluctuations during magnetic separation.
CHINESE CHEMICAL LETTERS
(2022)
Article
Engineering, Electrical & Electronic
Tsuyoshi Yoneyama, Akihiro Kuwahata, Toru Murayama, Loi Tonthat, Shin Yabukami, Yuya Sato, Yuji Teramura, Wakako Ikeda-Ohtsubo, Tomoyuki Ogawa
Summary: In this study, a novel method for directly adsorbing antibodies on the surface of magnetic nanoparticles without coating any proteins was proposed. The antibodies were able to detect bacteria bound to the produced antibody-MNPs based on the antigen-antibody reaction, showing potential for highly sensitive bacteria detection.
IEEE TRANSACTIONS ON MAGNETICS
(2022)
Article
Food Science & Technology
Fei Peng, Beibei Li, Shijiao Sun, Fang Mi, Ying Wang, Cunming Hu, Pengfei Geng, Lin Pang, Jiutong Li, Ming Guan
Summary: A fluorescence inner filtration immunoassay (FIFI) method was developed for the detection of clenbuterol, which is sensitive, efficient, and easy to operate.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Ying Ying, Xueyan Cui, Hui Li, Lingyi Pan, Ting Luo, Zhen Cao, Jing Wang
Summary: A direct competitive ELISA and a magnetic lateral flow immunoassay (MLFIA) were developed to detect residues of Halosulfuron-methyl (HM) in tomato and maize matrices and paddy water, respectively. These methods showed high sensitivity and accuracy in the rapid detection of HM residues.
Article
Multidisciplinary Sciences
Gheyath K. Nasrallah, Raniya Al-Buainain, Nadin Younes, Soha R. Dargham, Duaa W. Al-Sadeq, Mohamed Elhassan, Ibrahim Al-Shaar, Hadi M. Yassine, Laith J. Abu-Raddad, Mohamed M. Emara, Ahmed Ismail
Summary: HIV and Syphilis are common STIs that pose a concern and burden on healthcare systems. This study evaluated the diagnostic efficiency of the protocols used by the Medical Commission Department (MC) in Qatar for screening these infections.
Article
Chemistry, Analytical
Shuwen Lin, Xiaomei Hu, Jiayi Lin, Shunqin Wang, Jin Xu, Fan Cai, Jiancheng Lin
Summary: This study presents a sensitive pH-responsive point-of-care electrochemical immunoassay for quantitative monitoring of H1N1 influenza virus. The use of Ti3C2-MXene nanosheets enhances detection sensitivity and signal amplification, showing promising potential for point-of-care applications in diagnostics.
Article
Geochemistry & Geophysics
Charlotte Le Traon, Tomas Aquino, Camille Bouchez, Kate Maher, Tanguy Le Borgne
Summary: The study explores the impact of sub-scale chemical gradients on reaction kinetics using localized pulses of solute reacting with a solid or a dissolved species in excess as a case study. The combination of diffusion and reaction leads to effective kinetics that differ quantitatively and qualitatively from batch kinetics, resulting in a variety of reaction dynamics depending on the nonlinearity of the local kinetics. The findings offer new insights into interpreting imperfectly mixed lab experiments and integrating sub-scale concentration gradients in reactive transport models for field systems.
GEOCHIMICA ET COSMOCHIMICA ACTA
(2021)
Article
Computer Science, Interdisciplinary Applications
Tamas Pfeil, Blanka Herbaly
Summary: This article models polyclonal antibody-antigen reactions assuming constant free antibody clone concentrations and monovalent reactants using a system of linear differential equations. The positive invariance of the simplex of immunologically meaningful points is demonstrated, the unique equilibrium point is determined, and its global asymptotic stability is proven. Numerical simulations illustrate the obtained results.
MATHEMATICS AND COMPUTERS IN SIMULATION
(2022)
Article
Biochemistry & Molecular Biology
Nastaran Moradi, Samad Muhammadnejad, Hamid Delavari, Negin Pournoori, Mohammad Ali Oghabian, Hossein Ghafouri
Summary: The conjugation of anti-CD3 monoclonal antibody with Fe3O4 coated by carboxymethyl dextran (CMD) using cyanogen bromide (CNBr) was shown to efficiently separate CD3+ T lymphocytes from whole blood with high purity. The method can be utilized for cell sorting and targeted MRI imaging in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Electrical & Electronic
Mohd Mawardi Saari, Nurul Ain Nadzri, Mohd Aufa Hadi Putera Zaini, Nur Huda Ramlan, Keiji Tsukada
Summary: A new eddy current probe was proposed and fabricated to detect defects in high-permeability steel components. The probe utilized an array of miniature fluxgate sensors and axial inductors, and implemented a phase-sensitive detection technique to isolate the magnetization signal from the eddy current signal. The probe successfully characterized both vertically and horizontally oriented slits with varying depths.
IEEE TRANSACTIONS ON MAGNETICS
(2022)
Article
Engineering, Electrical & Electronic
Minoru Hayashi, Taisei Kawakami, Shoya Adachi, Jin Wang, Kenji Sakai, Toshihiko Kiwa, Keiji Tsukada, Yohei Miyamoto, Mikihito Hirohata, Toshiyuki Ishikawa
Summary: In this study, a sensor probe adapted to the shape of the welded joint was developed using unsaturated ac MFL (USAC-MFL), which is less affected by nonuniform magnetic permeability. The measurement results showed a strong correlation between the intensity of the MFL and the crack depth. The detection performance was evaluated using the receiver-operating characteristic (ROC) curve, and the metrics were all above 0.8.
IEEE TRANSACTIONS ON MAGNETICS
(2022)
Article
Engineering, Electrical & Electronic
Mohd Herwan Sulaiman, Zuriani Mustaffa, Mohd Mawardi Saari, Ahmad Johari Mohamad
Summary: This paper proposes a novel evolutionary optimization algorithm called BMO, which effectively solves the complex ORPD problem in power systems by applying the concepts of Hardy-Weinberg principle and sperm-cast process of barnacles. The obtained results show that BMO is effective in achieving minimum loss for ORPD problem.
PRZEGLAD ELEKTROTECHNICZNY
(2022)
Article
Optics
Jin Wang, Kosuke Sato, Yuichi Yoshida, Kenji Sakai, Toshihiko Kiwa
Summary: This study introduces the recent progress of a versatile terahertz chemical microscope (TCM) in the detection of small biomolecules, cancer cells, and antibody-antigen immunoassaying. The TCM is highlighted for its advantages in chemical and biosensing, such as label-free detection and high sensitivity. Furthermore, its new application in detecting low concentration histamine is demonstrated.
Article
Nanoscience & Nanotechnology
Keiji Tsukada, Minoru Hayashi, Taisei Kawakami, Shoya Adachi, Kenji Sakai, Toshihiko Kiwa, Toshiyuki Ishikawa, Mohd Mawardi Saari, Kengo Hori, Kazumasa Hisazumi, Tomonori Tominaga
Summary: The diagnosis and prevention of the deterioration of iron-steel infrastructure has become an important social issue. This study investigated the effect of electromagnetic characteristics on magnetic thickness measurement and improved the thickness estimation method. The experimental results showed that there were phase shifts and linearity differences in the calibration curves of different steel plates, which could be explained by studying the electromagnetic characteristics of the materials (permeability and conductivity).
Article
Biochemistry & Molecular Biology
Jin Wang, Kenji Sakai, Toshihiko Kiwa
Summary: Peptides derived from odorant-binding proteins (OBPs) were designed for recognition of SARS-CoV-2-related volatile organic compounds (VOCs) in exhaled breath. Computational docking and prediction tools were used to analyze the peptide characteristics. The results demonstrated a rational, rapid, and efficient approach to enhance biosensor performance for COVID-19 screening and other applications.
Article
Computer Science, Artificial Intelligence
Mohd Herwan Sulaiman, Zuriani Mustaffa, Mohd Mawardi Saari, Hamdan Daniyal, Seyedali Mirjalili
Summary: This paper introduces a new evolutionary algorithm called EMA, which employs random mating and crossover index to generate new offspring, and considers environmental factors as an exploratory mechanism. The algorithm is tested and compared, demonstrating its effectiveness in different search spaces and real optimization problems.
NEURAL COMPUTING & APPLICATIONS
(2023)
Article
Chemistry, Analytical
Feroz Ahmed, Masashi Shimizu, Jin Wang, Kenji Sakai, Toshihiko Kiwa
Summary: In this study, the optimization of microflow channels and the evaluation of different activation functions were carried out using a deep learning algorithm. The average accuracy values of three functions were obtained through trial and error.
Article
Engineering, Electrical & Electronic
K. Yamashita, K. Kishimoto, H. Kuroda, J. Wang, K. Sakai, M. M. Saari, T. Kiwa
Summary: This study aims to improve the accuracy of magnetic immunoassay (MIA) by evaluating the AC magnetic properties of magnetic nanoparticles (MNPs) in biological solvents. The developed HTS-SQUID magnetic immunoassay system was used to measure the magnetic signal of MNPs. The results show that the device instability does not affect the measurement of magnetic signal changes in MNPs caused by biological solvents. The magnetic signal of MNPs in biological solvents is affected by the aggregation of MNPs in the blood, and there are at least two steps in the mechanism of aggregation.
IEEE TRANSACTIONS ON APPLIED SUPERCONDUCTIVITY
(2023)
Article
Chemistry, Analytical
Shoya Adachi, Minoru Hayashi, Taisei Kawakami, Yuto Ando, Jin Wang, Kenji Sakai, Toshihiko Kiwa, Toshiyuki Ishikawa, Keiji Tsukada
Summary: Infrastructure facilities built 50 years ago have aged and caused severe corrosion and collapse of steel sheet pile wharfs. To solve this problem, non-destructive inspection techniques are needed. In this study, sensor probes with different coil diameters were developed to measure the thickness of steel sheet piles with high lift-off. The probes successfully obtained measurements at a lift-off of up to 60 mm.
Article
Nanoscience & Nanotechnology
Kouhei Kishimoto, Hiroto Kuroda, Misaki Tsubota, Kei Yamashita, Jin Wang, Kenji Sakai, Mohd Mawardi Saari, Toshihiko Kiwa
Summary: Magnetic nanoparticles (MNPs) are commonly used in medical applications, particularly in magnetic immunoassay (MIA) for pathological diagnosis. This study proposes a nonthermal dispersion method for MNPs using femtosecond pulse laser irradiation. The dispersion of MNPs was evaluated by measuring the change in magnetic signals. Results show that femtosecond pulse laser irradiation is a suitable method for dispersion of MNPs in MIA systems.
ACS APPLIED NANO MATERIALS
(2022)
Editorial Material
Optics
Toshihiko Kiwa, Masayoshi Tonouchi
Article
Engineering, Electrical & Electronic
Kota Nomura, Masaomi Washino, Tetsuya Matsuda, Shun Tonooka, Seino Satoshi, H. Yoshida, K. Nishigaki, Takashi Nakagawa, Toshihiko Kiwa
Summary: Magnetic particle imaging (MPI) is an imaging technique that detects the nonlinear responses of magnetic nanoparticles (MNPs) and achieves spatial encoding by saturating the magnetic moment of MNPs. The sensitivity of MPI has been improved by using a field-free line (FFL) that forms a field-free region as a line. A neodymium magnet and an iron yoke were used to develop an MPI with FFL device for imaging objects with a small amount of MNPs, such as in biological systems. The MPI equipment has been developed to detect amyloid-beta, a causative agent of Alzheimer's disease, by discriminating between bound and unbound magnetic particles based on the differences in relaxation times and studying the magnetization response of MNPs.
IEEE MAGNETICS LETTERS
(2023)
Proceedings Paper
Computer Science, Software Engineering
Zulkifly Aziz, Mohd Mawardi Saari, Mohd Aufa Hadi Putera Zaini, Nurul A'In Nadzri, Chin Sim Yee
Summary: This study examines a new method for calculating mutual inductance using the coil reactance approach in the finite element method. The results validate the effectiveness of this method in designing wireless couplers.
2022 IEEE 18TH INTERNATIONAL COLLOQUIUM ON SIGNAL PROCESSING & APPLICATIONS (CSPA 2022)
(2022)
