Article
Multidisciplinary Sciences
Wen-Yu Liu, Chun-Ping Yu, Chao-Kang Chang, Hsiang-June Chen, Meng-Yun Li, Yi-Hua Chen, Shin-Han Shiu, Maurice S. B. Ku, Shih-Long Tu, Mei-Yeh Jade Lu, Wen-Hsiung Li
Summary: The superior photosynthetic efficiency of C-4 leaves over C-3 leaves is due to their unique Kranz anatomy. This study identified the regulators and underlying molecular mechanisms of Kranz anatomy development in maize leaves. The researchers analyzed transcriptomic data and identified transcription factors and auxin transporter genes that are involved in early Kranz development. They constructed a gene regulatory network and provided insights into the regulation of early leaf development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Chiara M. A. Cefalo, Teresa Mezza, Andrea Giaccari, Rohit N. Kulkarni
Summary: This study compared four methods for RNA extraction and found that the RNeasy MicroKit + Carrier (Qiagen) method provided the highest yield and purity.
Article
Neurosciences
Amanda M. Canto, Alexandre H. B. Matos, Alexandre B. Godoi, Andre S. Vieira, Beatriz B. Aoyama, Cristiane S. Rocha, Barbara Henning, Benilton S. Carvalho, Vinicius D. B. Pascoal, Diogo F. T. Veiga, Rovilson Gilioli, Fernando Cendes, Iscia Lopes-Cendes
Summary: Research using a multi-omics approach in an animal model of mesial temporal lobe epilepsy revealed differences in the transcriptomic and proteomic profiles between the dentate gyrus (DG) and Cornu Ammonis 3 (CA3) of the hippocampus, suggesting enhanced epileptogenesis in the CA3 region and abnormalities mainly occurring in CA3. Abnormal regulation of excitatory neuronal mechanisms mediated by NMDA receptors, changes in serotonin signaling, and neuronal activity controlled by CaMK regulation and LRRK2/WNT signaling pathways are involved in the epileptogenesis in the pilocarpine model.
Article
Nutrition & Dietetics
Shirelle X. Liu, Tenille K. Fredrickson, Natalia Calixto Mancipe, Michael K. Georgieff, Phu V. Tran
Summary: Fetal-neonatal iron deficiency (ID) causes long-term neurocognitive and affective dysfunctions, with sex-specific effects that are poorly understood at the molecular level. In this study using a rat model, both ID and prenatal choline treatment induced sex-specific transcriptome alterations in the adult rat hippocampus. ID induced changes in gene networks related to enhanced neuroinflammation, while choline supplementation partially rescued ID-induced dysregulation and had both beneficial and adverse effects on hippocampal gene expression.
Article
Cell Biology
Xiaodan Zhang, Chuansheng Hu, Chen Huang, Ying Wei, Xiaowei Li, Miaomiao Hu, Hua Li, Ji Wu, Daniel M. Czajkowsky, Yan Guo, Zhifeng Shao
Summary: This method presents a robust approach for acquiring finely resolved transcriptional programs from clinical tissues using immunofluorescence-guided laser capture microdissection. It allows analysis of transcriptional networks and signaling pathways of specific cell types within native tissues. The use of a small molecule inhibitor, RVC, protects the RNA from degradation during the procedure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Xinyu Yang, Yinggai Song, TianYu Liang, Qiqi Wang, Ruoyu Li, Wei Liu
Summary: Coccidioidomycosis is increasing among Chinese patients, with challenges in diagnosis due to lack of specificity in clinical presentations and imaging findings. Diagnosis is primarily confirmed through histopathological examination, and the rarity of endemic mycoses along with limited availability of diagnostic tests poses challenges. The use of LCM and molecular techniques has shown improvement in species identification for timely diagnosis.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Oncology
Kristina Navrazhina, Sandra Garcet, Samuel C. Williams, Nicholas Gulati, Felix Kiecker, John W. Frew, Hiroshi Mitsui, James G. Krueger
Summary: The study demonstrates the importance of using laser capture microdissection (LCM) to accurately identify molecular changes in melanoma samples, without being affected by inflammatory infiltration. LCM analysis identified a greater number of differentially expressed genes, including melanocyte-specific genes, and revealed pathways related to cell proliferation.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Article
Plant Sciences
Wei Qin, Yongpeng Li, Bowen Peng, Hang Liu, Tiantian Chen, Xin Yan, Yaojie Zhang, Chen Wang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: In this study, a method using laser capture microdissection (LCM) was developed to isolate glandular secretory trichomes (GSTs) from leaf epidermis without fixation. The method efficiently captured GSTs and enriched for related metabolites. This approach enables the study of pure plant cell populations and has the potential to discover novel secondary metabolites.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Ganesh P. Pujari, Kiran K. Mangalaparthi, Benjamin J. Madden, Firdous A. Bhat, M. Cristine Charlesworth, Amy J. French, Gunveen Sachdeva, Eugenio Daviso, Ulrich Thomann, Patrick McCarthy, Sameer Vasantgadkar, Debadeep Bhattacharyya, Akhilesh Pandey
Summary: Lasercapture microdissection (LCM) is essential for proteomic analysis of specific regions from FFPE tissue samples. Automation of sample preparation workflows is still under development. This study presents a simplified and rapid workflow using adaptive focused acoustics (AFA) technology for high-throughput FFPE-based proteomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Plant Sciences
Liqing Lu, Haijian Yang, Yanhui Xu, Li Zhang, Juxun Wu, Hualin Yi
Summary: This study used RNA-seq and Pacbio-seq technologies to analyze the transcriptome and plant hormone signals in citrus seed development, revealing the important roles of hormone signaling, cell division, and nutrient metabolism processes. Additionally, several genes, including CrWRKY74, were found to play critical roles in seed abortion.
Article
Pharmacology & Pharmacy
Shashank Pandey, Zdenek Tuma, Tereza Smrhova, Miroslava Cedikova, Tereza Macanova, Magdalena Chottova Dvorakova
Summary: A platform for precise proteomic profiling of targeted cell populations from heterogeneous tissue sections is developed using LCM and CAP-IA integration, showing effectiveness for handling very small tissue sections.
Article
Biochemical Research Methods
Jongmin Woo, Madeline Schoenfeld, Xinguo Sun, Thierry Iraguha, Zhanxiang Zhou, Qibin Zhang
Summary: Using a simplified method, we conducted a proteomics study of intestinal tissue enriched with Paneth cells and identified several proteins associated with Paneth cell characteristics.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Xinjia Cai, Heyu Zhang, Jianyun Zhang, Tiejun Li
Summary: Oral submucous fibrosis (OSF) is a common potentially malignant disorder in the oral cavity, and researchers have proposed a method using laser capture microdissection to obtain epithelial and mesenchymal cells for studying the molecular mechanisms of this disease.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemical Research Methods
Firat Kaya, Matthew D. Zimmerman, Rosleine Antilus-Sainte, Martin Gengenbacher, Claire L. Carter, Veronique Dartois
Summary: This study developed a workflow that combines laser-capture microdissection and mass spectrometry imaging to investigate the spatial distribution and quantitation of small molecule drugs in bones. The optimized staining methods enabled simultaneous histology staining and mass spectrometry imaging, revealing drug accumulation in bone tissue architecture.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Wenbo Guo, Yining Hu, Jingyang Qian, Lidan Zhu, Junyun Cheng, Jie Liao, Xiaohui Fan
Summary: Spatial omics technologies provide valuable insights into cells and tissues within a complex context, enhancing our understanding of complex biological systems. Laser capture microdissection (LCM) is an advanced method that quickly collects regions of interest (ROIs) from heterogeneous tissues, and is widely used for studying the cellular and molecular mechanisms of diseases.
JOURNAL OF GENETICS AND GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Sung Jin Kim, Sang Hyuk Lee, Heesu Lee, Myoung-Sook Shin, Jae Wook Lee
Summary: This study aimed to synthesize and evaluate the anti-inflammatory activity of 3-substituted-indolin-2-one derivatives. Among the nineteen derivatives synthesized, 3-(3-hydroxyphenyl)-indolin-2-one showed the highest anti-inflammatory activity by inhibiting nitric oxide production, suppressing TNF-alpha and IL-6 levels, and inhibiting LPS-induced signal pathways. The findings suggest that 3-(3-hydroxyphenyl)-indolin-2-one possesses excellent anti-inflammatory activity and should be further investigated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Stephen D. Ginsberg, Sahil Sharma, Larry Norton, Gabriela Chiosis
Summary: Diseases are complex manifestations of changes in protein-protein interaction networks caused by stressors, genetics, environment, and their combinations, affecting molecular interactions and disrupting the normal function of cells, tissues, and organisms. Disrupting epichaperomes, which enable dysfunctional rewiring of these networks, is proposed as a mechanism for reverting context-specific dysfunction to a normative state. This has potential implications for precision medicine in detecting and treating complex diseases like cancer and neurodegenerative disorders.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Psychiatry
Sang Jin Rhee, Dongyoon Shin, Daun Shin, Yoojin Song, Eun-Jeong Joo, Hee Yeon Jung, Sungwon Roh, Sang-Hyuk Lee, Hyeyoung Kim, Minji Bang, Kyu Young Lee, Se Hyun Kim, Minah Kim, Jihyeon Lee, Jaenyeon Kim, Yeongshin Kim, Jun Soo Kwon, Kyooseob Ha, Youngsoo Kim, Yong Min Ahn
Summary: Data-driven approaches are crucial for understanding the heterogeneity and overlap in transdiagnostic samples. This study used plasma proteomics-based clustering to subtype patients with psychotic-affective disorders. Significant differences were found between patients and healthy controls in terms of pathologic peptides. Latent class analysis revealed distinct classes of patients, with the negative symptom factor being significantly different between the classes. This highlights the significance of negative symptoms in understanding the heterogeneity and overlap of psychotic-affective disorders.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Psychology, Clinical
Hyun-Ju Kim, Hye-Yeon Jung, Minji Bang, Chongwon Pae, Sang-Hyuk Lee
Summary: This study investigated the clinical characteristics of suicide attempt in patients with panic disorder. The results showed that patients with a history of suicide attempt had more severe panic symptoms and poorer response to pharmacological treatment. Fear of cognitive dyscontrol was identified as the central symptom in the network of patients with both panic disorder and suicide attempt.
DEPRESSION AND ANXIETY
(2023)
Article
Biochemistry & Molecular Biology
Melissa J. Alldred, Harshitha Pidikiti, Adriana Heguy, Panos Roussos, Stephen D. Ginsberg
Summary: Basal forebrain cholinergic neuron (BFCN) degeneration is a characteristic of Down syndrome (DS) and Alzheimer's disease (AD). Maternal choline supplementation (MCS) has been shown to attenuate the degeneration of BFCNs in a mouse model, suggesting a potential therapeutic effect for DS and AD.
Article
Psychiatry
Hyun-Ju Kim, Minji Bang, Chun Il Park, Chongwon Pae, Sang-Hyu Lee
Summary: This study aimed to compare the symptom severities and overall function before and during the COVID-19 pandemic among patients with panic disorder seeking treatment. The results showed that patients enrolled during COVID-19 exhibited higher levels of interoceptive fear and lower overall functioning. It was also found that agoraphobia and avoidance had significantly higher strength and expected influence in patients with panic disorder during COVID-19.
PSYCHIATRY INVESTIGATION
(2023)
Correction
Multidisciplinary Sciences
Hyun-Ju Kim, Junghwa Seo, Minji Bang, Sang-Hyuk Lee
SCIENTIFIC REPORTS
(2023)
Article
Psychiatry
Sang Jin Rhee, Dongyoon Shin, Daun Shin, Yoojin Song, Eun-Jeong Joo, Hee Yeon Jung, Sungwon Roh, Sang-Hyuk Lee, Hyeyoung Kim, Minji Bang, Kyu Young Lee, Jihyeon Lee, Jaenyeon Kim, Yeongshin Kim, Youngsoo Kim, Yong Min Ahn
Summary: This study quantified the plasma proteomes of 299 MDD or BD patients and revealed that childhood trauma and overeating symptoms were significantly associated with plasma protein networks, suggesting their importance as endophenotypes in affective disorders.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Psychiatry
Min-Jung You, Chan Rim, Minji Bang, Soyoung Sung, Hui-Ju Kim, Sang-Hyuk Lee, Min-Soo Kwon
Summary: This study found that microglia in patients with panic disorder have unique characteristics, including dysregulation of the cholesterol biosynthesis pathway and impaired phagocytosis, which reflect their clinical phenotype.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Medicine, General & Internal
Melissa J. J. Alldred, Stephen D. D. Ginsberg
Summary: Single-cell and single-population RNA sequencing is a valuable tool in studying the transcriptomic profiles of neurons based on their spatial localization. Laser capture microdissection and RNA purification allow for the isolation of desired neurons for downstream analysis, such as differential gene expression and pathway exploration.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Clinical Neurology
Hyun-Ju Kim, Sang-Hyuk Lee, Chongwon Pae
Summary: This study investigated gender differences in panic disorder (PD) using a network approach. The results showed that agoraphobia was the most central symptom in men, while loss of pleasure was the most central symptom in women. Furthermore, there were gender differences in the neural correlates of white matter.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Multidisciplinary Sciences
Yeong-Geon Hwang, Chongwon Pae, Chae Rim Song, Hyun-Ju Kim, Minji Bang, Chun Il Park, Tai Kiu Choi, Min-Kyoung Kim, Sang-Hyuk Lee
Summary: Self-compassion involves taking a positive attitude towards oneself when suffering. This study found that levels of self-compassion were negatively correlated with white matter microstructural changes in the superior longitudinal fasciculus (SLF) in healthy individuals. The self-kindness and mindfulness subscale scores were also negatively correlated with these microstructural changes.
SCIENTIFIC REPORTS
(2023)
Article
Nutrition & Dietetics
Yeong-Geon Hwang, Chongwon Pae, Sang-Hyuk Lee, Ki-Hwan Yook, Chun Il Park
Summary: This study investigated the relationship between adherence to the Mediterranean diet and depression in the South Korean population. The results showed that individuals who adhered to the Mediterranean diet were less likely to report depression, which is significant for Asian populations.
FRONTIERS IN NUTRITION
(2023)
Article
Neurosciences
Hyun-Ju Kim, Minji Bang, Chun Il Park, Sang-Hyuk Lee
Summary: The study investigated the association between stressful life events and the level of 5-HTTLPR methylation in patients with panic disorder (PD), as well as the white matter alterations in trauma-related regions. PD patients showed lower levels of DNA methylation at 5-HTTLPR 5 CpG sites compared to healthy controls. The DNA methylation levels were negatively associated with parental separation-related psychological distress and positively correlated with fractional anisotropy values of the superior longitudinal fasciculus (SLF), which may be related to trait anxiety.
NEUROPSYCHOBIOLOGY
(2023)
Article
Psychiatry
Sungjun Ahn, Sang-Hyuk Lee, Kang Soo Lee
Summary: This study investigated the impact of intolerance of uncertainty on brain structural changes and symptom severity in patients with panic disorder. The findings revealed that increased intolerance of uncertainty was associated with cortical thinning in the lingual gyrus and increased gray matter volume in the pars triangularis. These structural alterations were correlated with symptoms of depression and anxiety.
PSYCHIATRY INVESTIGATION
(2023)