Article
Materials Science, Multidisciplinary
Ipshita Menon, Maryam Zaroudi, Yuanzhe Zhang, Elizabeth Aisenbrey, Liwei Hui
Summary: Lipid nanoparticles have promising applications as non-viral vectors for targeted drug and gene delivery. This review focuses on the techniques to fabricate active targeting lipid nanoparticles, including one-pot assembly and post-insertion functionalization. The advantages, restrictions, and challenges in formulation screening and clinical translation are discussed.
MATERIALS TODAY ADVANCES
(2022)
Review
Pharmacology & Pharmacy
Laxmikant Gautam, Priya Shrivastava, Bhavana Yadav, Anamika Jain, Rajeev Sharma, Sonal Vyas, S. P. Vyas
Summary: This review discusses the recent developments and clinical potential of multicompartment systems called vesosomes, as well as their preparation methods, surface modifications, and potential for site-specific drug delivery. The focus is on the possibility of vesosome accumulation mediated by the reticuloendothelial system (RES) and their application in tumor targeting, which are areas for further exploration.
DRUG DISCOVERY TODAY
(2022)
Article
Engineering, Biomedical
Yoshiro Tahara, Riko Mizuno, Tomoki Nishimura, Sada-atsu Mukai, Rie Wakabayashi, Noriho Kamiya, Kazunari Akiyoshi, Masahiro Goto
Summary: Biomaterial-based immunoengineering is one of the most attractive research fields in recent years. A novel vaccine carrier, S/O/W emulsion, consisting of similar materials to an approved emulsion adjuvant, was prepared and shown to produce antigen-specific antibodies and enhance antitumor effects in vivo.
Article
Chemistry, Multidisciplinary
Wen Yang, Lin Wang, Mulin Fang, Vinit Sheth, Yushan Zhang, Alyssa M. Holden, Nathan D. Donahue, Dixy E. Green, Alex N. Frickenstein, Evan M. Mettenbrink, Tyler A. Schwemley, Emmy R. Francek, Majood Haddad, Md Nazir Hossen, Shirsha Mukherjee, Si Wu, Paul L. DeAngelis, Stefan Wilhelm
Summary: In this study, we explored the use of self-polysaccharide heparosan (HEP) as a surface modification for nanoparticles. Our findings showed that HEP-coating reduced the protein corona formation and increased the uptake of nanoparticles by certain innate immune cells without causing cytotoxicity.
Article
Chemistry, Multidisciplinary
Maximilian Regenold, Kan Kaneko, Xuehan Wang, H. Benson Peng, James C. Evans, Pauric Bannigan, Christine Allen
Summary: Triggered drug delivery strategies, such as thermosensitive liposomes, have shown improved targetability for chemotherapeutics. However, most studies have focused on doxorubicin encapsulation, while other drugs could also benefit from this targeted delivery approach.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Ming Wu, Qizhi Wang, Sai Chen, Zhanwei Zhou, Jing Li, Honghao Sun, Jiali Liu, Guangji Wang, Fang Zhou, Minjie Sun
Summary: This study investigated the dual inhibition of metabolic plasticity in breast cancer cells using Lip@GOx&Tel, which targeted and inhibited two nutrient supply mechanisms. This led to a reduction in ATP production and biosynthesis precursors necessary for tumor growth, resulting in anti-tumor effects. Additionally, Lip@GOx&Tel disrupted redox homeostasis, further enhancing the therapeutic effect. The study also addressed the limitation of combining protein drugs and small molecule drugs in vivo by using liposome nanoparticles. Overall, this work provides a new perspective in nanomedicine for metabolic intervention in diseases.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Immunology
Lirong Mao, Lifa Xu, Xiaochun Wang, Yingru Xing, Jian Wang, Yanpeng Zhang, Wei Yuan, Jianpeng Du, Zilun Shi, Jilei Ma, Jingyan Zhang, Xiaohan Zhang, Xinping Wang
Summary: COVID-19 has negatively impacted the progress in tuberculosis prevention and treatment, resulting in increased mortality. This study introduces a novel strategy to overcome the limitations of the Bacille Calmette-Guérin (BCG) vaccine in preventing latent infection and reactivation of tuberculosis. By selecting the latency antigen Rv0572c, the rRv0572c/DMT vaccine was developed as a potential booster for BCG, demonstrating the ability to stimulate a specific immune response against TB in both human and mouse models.
Article
Chemistry, Multidisciplinary
Xuedan He, Shiqi Zhou, Wei-Chiao Huang, Amal Seffouh, Moustafa T. Mabrouk, M. Thomas Morgan, Joaquin Ortega, Scott Abrams, Jonathan F. Lovell
Summary: A new vaccine adjuvant system was developed to induce strong cellular immune responses against multiple tumor cell lines, demonstrating durable immunity. The system showed the importance of stable particle formation for effective immune response induction and could control local and metastatic disease in a therapeutic setting.
Article
Medicine, Research & Experimental
Ting Su, Shurong Zhou, Suling Yang, Nicholas Humble, Fuwu Zhang, Guocan Yu, Paula D. Bos, Furong Cheng, Kristoffer Valerie, Guizhi Zhu
Summary: This study reports a radioimmunotherapy for adult brain cancer that combines radiotherapy, immune checkpoint blockade, and lymph-node-targeting vaccines. The vaccines induced anti-cancer immune responses in mice and enhanced therapeutic efficacy in orthotopic brain cancer mice. However, radioimmunotherapy only showed a trend towards improved efficacy compared to radiotherapy alone.
Article
Biotechnology & Applied Microbiology
Robert Castro, Elizabeth Aisenbrey, Liwei Hui
Summary: This study investigated the influence of four different formulation methods on the presentation of surface ligands on lipid nanoparticles (LNPs). The results showed that the formulation methods could modulate the expression of targeting ligands on LNPs, providing guidance for future formulation screening and nanomedicine engineering.
Article
Biochemistry & Molecular Biology
Nadia Moqbel Hassan Alzubaydi, Zainab Oun Ali, Sura Al-Asadi, Rusul Al-kahachi
Summary: In this study, a multi-epitope vaccine (MEV) targeting all strains of C. abortus was designed using bioinformatics and immunoinformatics approaches. Highly antigenic and non-allergic proteins were identified and incorporated into the MEV construct. Despite being in silico findings, further wet lab investigations are crucial to validate the safety and efficacy of the vaccine.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Immunology
Melika Moradi, Farzaneh Vahedi, Arian Abbassioun, Arash Ramezanpour Shahi, Mohammad Sholeh, Mortaza Taheri-Anganeh, Zahra Dargahi, Roya Ghanavati, Seyyed Hossein Khatami, Ahmad Movahedpour
Summary: According to the World Health Organization, 10 million people worldwide are infected with tuberculosis (TB), resulting in 1.5 million deaths, with 214,000 individuals co-infected with HIV. The development of an effective TB vaccine is crucial due to the high infection rate. Various methodologies, including the protein subunit vaccine, have been proposed, demonstrating higher protection than Bacillus culture vaccines. The liposomal adjuvant system is a safe and efficient method for TB vaccination, as well as for other intracellular infections and malignancies.
IMMUNITY INFLAMMATION AND DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Yisi Tang, Wufa Fan, Guihua Chen, Meng Zhang, Xueping Tang, Hairui Wang, Pengfei Zhao, Qin Xu, Zhibing Wu, Xiaoxi Lin, Yongzhuo Huang
Summary: The study demonstrated that a nanovaccine can induce potent anti-tumor activity in various cancer models by targeting tumor-associated macrophages (TAM) and remodeling tumor microenvironment. This nanovaccine is a simple, safe, and robust strategy for cancer immunotherapy with general applicability across different cancer types by targeting TAM and remodeling tumor microenvironment.
Article
Immunology
Teerasit Techawiwattanaboon, Thomas Courant, Livia Brunner, Suwitra Sathean-anan-kun, Pratomporn Krangvichian, Nutta Iadsee, Yaowarin Nakornpakdee, Noppadon Sangjun, Pat Komanee, Nicolas Collin, Kiat Ruxrungtham, Kanitha Patarakul
Summary: This study evaluated the potential of four adjuvants in leptospirosis vaccines and found that LQ, LQuil, and SQuil adjuvants performed similarly to the LMQ adjuvant in enhancing immune protection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Katy McCann, Adrian von Witzleben, Jaya Thomas, Chuan Wang, Oliver Wood, Divya Singh, Konstantinos Boukas, Kaidre Bendjama, Nathalie Silvestre, Finn Cilius Nielsen, Gareth Thomas, Tilman Sanchez-Elsner, Jason Greenbaum, Stephen Schoenberger, Bjoern Peters, Pandurangan Vijayanand, Natalia Savelyeva, Christian Ottensmeier
Summary: This study demonstrates the feasibility of efficiently identifying tumor-specific neoantigens and targeting them with vaccination in tumors with a low mutational burden, showing promising potential for successful clinical exploitation.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Biochemistry & Molecular Biology
Aiva Plotniece, Arkadij Sobolev, Claudiu T. Supuran, Fabrizio Carta, Fredrik Bjoerkling, Henrik Franzyk, Jari Yli-Kauhaluoma, Koen Augustyns, Paul Cos, Linda De Vooght, Matthias Govaerts, Juliana Aizawa, Paeivi Tammela, Raivis Zalubovskis
Summary: Natural products and analogues are valuable sources for the discovery of antibacterial drugs. The identification of bacterial metalloenzymes and the synthesis of selective inhibitors are interesting for the development of antibacterial agents due to increasing drug resistance. Peptide nucleic acids represent a novel strategy for targeting pathogens through antisense inhibition and development of antisense peptide nucleic acids. The review also discusses alternative therapeutic options and optimized in vitro and in vivo models for studying biofilm-related infections along with an overview of drug delivery nanosystems.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Xiaoyue Xu, Thomas Rades, Holger Grohganz
Summary: It has been observed that water, as a plasticizer, can increase molecular mobility and decrease the glass transition temperature (Tg) in amorphous systems. However, a recent study found an anti-plasticizing effect of water on prilocaine (PRL), which could be used to moderate the plasticizing effect of water in co-amorphous systems. Nicotinamide (NIC) can form co-amorphous systems with PRL. In this study, the Tgs and molecular mobility of hydrated co-amorphous NIC-PRL systems were compared with those of anhydrous systems to investigate the effect of water.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Katharina Holzapfel, Thomas Rades, Claudia S. Leopold
Summary: The influence of an enantiomeric co-former and the preparation method on the solid-state properties and physical stability of co-amorphous systems was investigated in this study. It was found that the chirality of the co-former and the preparation method had an impact on the solid-state properties and physical stability of the systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Editorial Material
Pharmacology & Pharmacy
Shyamal Das, Thomas Rades, Arlene McDowell
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Anas Aljabbari, Abhijeet Girish Lokras, Jacob Judas Kain Kirkensgaard, Thomas Rades, Henrik Franzyk, Aneesh Thakur, Yibang Zhang, Camilla Foged
Summary: We investigated the structural and material properties of siRNA-loaded LPNs using various analytical techniques. The results revealed the self-assembly of lipidoid into lamellar structures, the lateral organization of siRNA between lipidoid bilayers, and the core-shell structure of siRNA-loaded LPNs. The interactions between lipidoid and siRNA were observed, and the inclusion of siRNA affected the phase behavior of lipidoid. These findings contribute to the understanding of siRNA-loaded LPNs and their applications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Ditlev Birch, Edward J. Sayers, Malene V. Christensen, Arwyn T. Jones, Henrik Franzyk, Hanne M. Nielsen
Summary: This study compared the membrane association, cellular uptake, and delivery capacity of all-l and all-d enantiomers of penetratin in different cell models and cargos. The results showed that d-PEN had tighter membrane binding and was superior in delivering hydrophilic peptide cargoes across the Caco-2 epithelium compared to l-PEN. However, there was no enhanced delivery of the hydrophobic cyclosporin, and both enantiomers had similar intracellular insulin uptake.
Article
Pharmacology & Pharmacy
Marco Herrera-Barrera, Milan Gautam, Abhijeet Lokras, Kseniia Vlasova, Camilla Foged, Gaurav Sahay
Summary: Prime editing is an advanced gene editing platform that can correct almost any disease-causing mutation. This study developed lipid nanoparticles as delivery vehicles for prime editors, which resulted in improved efficiency and speed of editing. The use of these nanoparticles has the potential to revolutionize gene therapy and open up new possibilities for treatment.
Article
Chemistry, Multidisciplinary
Jessica Pihl, Thomas M. M. Clausen, Jiarong Zhou, Nishta Krishnan, Maj S. S. Orum-Madsen, Tobias Gustavsson, Robert Dagil, Mads Daugaard, Swati Choudhary, Camilla Foged, Jeffrey D. D. Esko, Liangfang Zhang, Ronnie H. H. Fang, Ali Salanti
Summary: Malaria-infected erythrocytes and oncofetal CS have distinct tropism and can potentially be used for cancer targeting. Researchers have developed a drug delivery platform using erythrocyte membrane-coated nanoparticles that mimic malaria-infected erythrocytes and specifically target and kill melanoma cells. This biomimetic approach shows potential as a broadly targeted cancer therapy.
Article
Pharmacology & Pharmacy
Jacob Rune Jorgensen, Wolfgang Mohr, Matthias Rischer, Andreas Sauer, Shilpa Mistry, Thomas Rades, Anette Muellertz
Summary: The use of amorphous solid dispersions (ASDs) is a commonly applied formulation strategy to improve the oral bioavailability of poorly water-soluble drugs. However, establishing a predictive model for the in vitro-in vivo relationship (IVIVR) has been challenging. This study suggests that drug absorption may be overestimated in in vitro dissolution-permeation (D/P) setups, and proposes a modified D/P setup to improve predictability by avoiding direct dissolution of drug particles in the permeation barrier.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Aneesh Thakur, Abishek Wadhwa, Abhijeet Lokras, Olivia Amanda Oest Mullertz, Dennis Christensen, Henrik Franzyk, Camilla Foged
Summary: The incorporation of ionizable lipid L5N12 in liposomal cationic adjuvant CAF (R) 09 maintains the adjuvant function and induces antigen-specific immune responses comparable to unmodified CAF (R) 09.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Marie Hellfritzsch, Dennis Christensen, Camilla Foged, Regina Scherliess, Aneesh Thakur
Summary: Reconstituted dry powder formulations of ZnO nanoparticles can induce antigen-specific antibodies and can be used in vaccines to enhance antigen-specific humoral immune responses against subunit protein antigens.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Mengwei Wang, Junbo Gong, Thomas Rades, Ines C. B. Martins
Summary: Ball milling can reduce the particle size of pharmaceutical powders and induce changes in their physical properties. In this study, we prepared three crystal forms of furosemide and used X-ray diffraction and modulated differential scanning calorimetry to characterize the samples. We found that forms I and III directly converted into an amorphous phase, while form II first underwent a polymorphic transition to form I and then gradually lost its crystallinity. The structural relaxation time of the amorphous fraction was longer for forms I and II, while it was shorter for form III, indicating an amorphous-to-amorphous transformation phenomenon.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Anas Aljabbari, Shinji Kihara, Thomas Rades, Ben J. Boyd, Karlis Berzins
Summary: The adsorption of gut relevant biomolecules onto particles after oral administration of solid oral dosage forms is expected to form a gastrointestinal corona, which could influence solution-mediated solid-state transformations. This study used low-frequency Raman spectroscopy to investigate the in situ solid-state phase transformations under biorelevant conditions and the presence of biomolecules. The results showed a change in the solid-state transformation pathway from amorphous to crystalline drug and the role of biomolecules in influencing these transformations.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Ines C. B. Martins, Anders S. Larsen, Anders O. Madsen, Olivia Aalling Frederiksen, Alexandra Correia, Kirsten M. O. Jensen, Henrik S. Jeppesen, Thomas Rades
Summary: Polyamorphism is a controversial solid-state phenomenon that is still not fully understood. In this study, three distinct polyamorphic forms of hydrochlorothiazide were discovered and their interconversions were investigated. Molecular dynamics simulations revealed differences in dihedral angle distributions between two of the polyamorphs, which may explain their different physicochemical properties.
Article
Chemistry, Applied
Laura Maria Duran Gleriani Primo, Cesar Augusto Roque-Borda, Christian Shleider Carnero Canales, Icaro Putinhon Caruso, Isabella Ottenio de Lourenco, Vitoria Maria Medalha Colturato, Rafael Miguel Sabio, Fernando Alves de Melo, Eduardo Festozo Vicente, Marlus Chorilli, Hernane da Silva Barud, Paula Aboud Barbugli, Henrik Franzyk, Paul Robert Hansen, Fernando Rogerio Pavan
Summary: This study investigates the use of antimicrobial peptide (AMP)-grafted antibiotic-loaded nanoparticles as a potential treatment for multidrug-resistant tuberculosis. The results show that the modified nanoparticles effectively inhibit the growth of drug-resistant tuberculosis strains, suggesting that this approach could be a powerful tool for revitalizing drugs against such strains.
CARBOHYDRATE POLYMERS
(2024)