Article
Agriculture, Dairy & Animal Science
Sangdon Ryu, Kiyeop Kim, Dae-Yeon Cho, Younghoon Kim, Sejong Oh
Summary: A new bacteriocin-producing lactic acid bacteria strain, Lactococcus lactis JNU 534, isolated from kimchi, was found to have preservative properties for animal-origin foods. The complete genome sequence of JNU 534 consists of one circular chromosome, one circular plasmid, and one circular contig sequence, with different GC contents.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Justina Mileriene, Jurgita Aksomaitiene, Kristina Kondrotiene, Tora Asledottir, Gerd Elisabeth Vegarud, Loreta Serniene, Mindaugas Malakauskas
Summary: Safety is paramount in the food industry for any substance or microorganism used. Whole-genome sequencing confirmed that the indigenous dairy isolate LL16 is Lactococcus lactis subsp. lactis. It exhibited non-pathogenicity and the absence of genes associated with antimicrobial resistance, virulence, and biogenic amine formation. Additionally, it showed potential for producing bacteriocins and the neurotransmitter GABA, making it a functional strain suitable for the dairy sector.
Article
Microbiology
Pavelas Sazinas, Jan Martinussen
Summary: This study reports the complete genome sequence of Lactococcus lactis AH1 strain isolated from viili, a Finnish dairy product. The strain is well-known for its production of exopolysaccharides, which contributes to the extreme viscosity of fermented milk.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Xing Wan, Timo M. Takala, Mingqiang Qiao, Per E. J. Saris
Summary: The genome sequence of Lactococcus lactis subsp. lactis N8, a nisin producer isolated in Finland in the 1960s, consists of a 2.42-Mb chromosome and two plasmids of 80.3 and 71.3 kb.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Jungmin Lee, Sojeong Heo, Jihoon Choi, Minsoo Kim, Eunji Pyo, Myounghee Lee, Sangick Shin, Jaehwan Lee, Jaehun Sim, Do-Won Jeong
Summary: Comparative genomic analysis showed that L. lactis may be the best species for glutamic acid production among eight LAB species. Strain HY7803 of L. lactis was selected for its high production of glutamic acid, especially when supplemented with citrate. Principal component analysis revealed differences in amino acid profiles among strains, highlighting HY7803's potential as a starter strain for glutamic acid production.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jonas Chodorski, Jan Hauth, Dorina Strieth, Andreas Wirsen, Roland Ulber
Summary: Despite being an important topic in biofilm research, we still know little about diffusion in biofilms. The study shows that external factors can affect the diffusion behavior and growth rate of biofilms, while also revealing differences in diffusion inside biofilms depending on their position in the flow-cell.
ENGINEERING IN LIFE SCIENCES
(2021)
Article
Microbiology
Nikita Zrelovs, Andris Dislers, Andris Kazaks
Summary: Researchers isolated a unique phage, Nocturne116, from a deceased local moth specimen, revealing a genome length of 25,554 bp with 52 predicted open reading frames. Despite the availability of numerous complete genomes of Lactococcus phages, Nocturne116 stands out as a novel genus due to its distinct genetic characteristics.
Article
Microbiology
Wanjin Qiao, Fulu Liu, Xing Wan, Yu Qiao, Ran Li, Zhenzhou Wu, Per Erik Joakim Saris, Haijin Xu, Mingqiang Qiao
Summary: This study sequenced and analyzed the whole genome of Lactococcus lactis, finding that it contains large plasmids and prophage-related fragments and has the potential to synthesize novel secondary metabolites. Additionally, a simplified genome chassis of L. lactis was successfully constructed, laying the foundation for its application in synthetic biology.
Article
Microbiology
Przemyslaw Salanski, Magdalena Kowalczyk, Jacek K. Bardowski, Agnieszka K. Szczepankowska
Summary: This study explored the potential use of Lactococcus lactis strains in the treatment of gastrointestinal cancer. The results showed that these strains inhibited the proliferation of human colorectal adenocarcinoma cells and triggered gene expression in the cells. Additionally, the strains demonstrated probiotic potential, tolerating bile salts and acid exposure, and exhibiting adherence properties. Genetic analysis revealed functional and safety-related features of the strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
O-Hyun Ban, Won Yeong Bang, Hyeon Ji Jeon, Young Hoon Jung, Jungwoo Yang, Dong Hyun Kim
Summary: In this study, the safety of Bifidobacterium lactis IDCC 4301 (BL) was investigated and it was found to be safe for consumption as a probiotic. BL was susceptible to most antibiotics and did not show any toxic effects. Additionally, BL exhibited strong antipathogenic activity. These findings suggest that BL can be used as a safe probiotic in various industries.
FOOD SCIENCE & NUTRITION
(2023)
Article
Fisheries
Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture
Summary: The whole genome sequence of Lactococcus garvieae Lg-per, originally isolated from rainbow trout in Turkey, was analyzed. The study revealed that Lg-per was actually Lactococcus petauri, not L. garvieae as previously believed. The 16S rRNA-based PCR detection method was found to be inadequate for identifying Lactococcus strains. This is the first report of L. petauri in Turkey and highlights the importance of using whole genome sequencing for analyzing L. garvieae isolates in other countries.
JOURNAL OF FISH DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Maciej Wnuk, Anna Lewinska
Summary: IFC combines microscopy with flow cytometry for accurate and rapid microbial profiling, making it a potentially useful method for screening bacterial species in milk samples.
FOOD AND BIOPRODUCTS PROCESSING
(2021)
Article
Microbiology
Javier Fernando Melo-Bolivar, Ruth Yolanda Ruiz Pardo, Howard Junca, Hanna Evelina Sidjabat, Juan Andres Cano-Lozano, Luisa Marcela Villamil Diaz
Summary: This study investigated a continuous-flow competitive exclusion culture originating from the Tilapia gut microbiome, identifying potential probiotic strains possibly belonging to novel genus/species taxa inside Lactococcus spp. and Priestia spp. The strains showed antibacterial activities and antibiotic susceptibility patterns, with uncharacterized genes that could play a role in antagonism to competing strains or specific symbiotic adaptations to the fish host. Overall, the study demonstrated the effectiveness of CFCEC in enriching and isolating strains with probiotic potential.
Article
Biochemistry & Molecular Biology
Wenhui Li, He Li, Yinxiao Zhang, Lijuan He, Chi Zhang, Xinqi Liu
Summary: The study found that digested soybean peptides promoted the growth and metabolism of Bifidobacterium animalis subsp. animalis JCM 1190, surpassing the effects of soybean protein. By regulating various metabolic pathways, digested soybean peptides enhanced the competitiveness of Bifidobacterium animalis subsp. animalis JCM 1190 in co-culture with Escherichia coli JCM 1649.
Article
Biotechnology & Applied Microbiology
Omid Ardalani, Ehsan Motamedian, Javad Hamedi
Summary: In this study, a genome-scale metabolic model of non-dairy Lactococcus lactis subsp. lactis NCDO 2118 named iOA1084 was reconstructed and validated. The validated model successfully predicted growth rates on different defined media and simulated flux distribution through metabolic pathways. It was found that an increase in glutamate uptake rate led to higher GABA production rates, affecting cell proton balance.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Virology
Magdalena Chmielewska-Jeznach, Jacek K. Bardowski, Agnieszka K. Szczepankowska
Article
Microbiology
Jakub Boreczek, Dorota Litwinek, Joanna Zylinska-Urban, Dariusz Izak, Krzysztof Buksa, Jan Gawor, Robert Gromadka, Jacek Karol Bardowski, Magdalena Kowalczyk
Article
Virology
Aleksandra Glowacka-Rutkowska, Magdalena Ulatowska, Joanna Empel, Magdalena Kowalczyk, Jakub Boreczek, Malgorzata Lobocka
Article
Biochemistry & Molecular Biology
Aleksandra Tymoszewska, Tamara Aleksandrzak-Piekarczyk
Summary: The study investigated the development of resistance to aureocin A53- and enterocin L50-like bacteriocins in bacterium Lactococcus lactis, and found that decreased sensitivity to membrane-active bacteriocins and antibiotics may lead to an increased vulnerability to other antibiotics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Ecology
Jakub Grzesiak, Aleksandra Woltynska, Marek K. Zdanowski, Dorota Gorniak, Aleksander swiatecki, Maria A. Olech, Tamara Aleksandrzak-Piekarczyk
Summary: The study conducted a functional diversity assessment of the microbial community on a specific Antarctic lichen, revealing that the characteristics of the lichen thalli growth sites impact the metabolic diversity of the bacterial communities.
Article
Microbiology
Anna Muszewska, Alicja Okrasinska, Kamil Steczkiewicz, Olga Drgas, Malgorzata Orlowska, Urszula Perlinska-Lenart, Tamara Aleksandrzak-Piekarczyk, Katarzyna Szatraj, Urszula Zielenkiewicz, Sebastian Pilsyk, Ewa Malc, Piotr Mieczkowski, Joanna S. Kruszewska, Przemyslaw Bernat, Julia Pawlowska
Summary: The study focused on the genomes and experimental characteristics of six Mucoromycotina fungi, revealing different traits among them and highlighting the presence of an additional bacterium in Thamnidium elegans. Despite subtle differences in carbon source preferences and enzyme capabilities, all tested Mucoromycotina fungi share common features such as the production of specific fatty acids and cell wall components.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Aleksandra Tymoszewska, Kirill Ovchinnikov, Dzung B. Diep, Malgorzata Slodownik, Edyta Maron, Beatriz Martinez, Tamara Aleksandrzak-Piekarczyk
Summary: Resistance of Lactococcus lactis to aureocin A53- and enterocin L50-like bacteriocins is associated with mutations in genes encoding ABC transporter and TCS, leading to upregulation of other operons. Protein modification, rather than absence, is critical for the development of resistance to bacteriocins.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Anna Berthold-Pluta, Monika Garbowska, Ilona Stefanska, Lidia Stasiak-Rozanska, Tamara Aleksandrzak-Piekarczyk, Antoni Pluta
Summary: The study aimed to determine the presence and species identification of Cronobacter spp. in nuts, seeds, and dried fruits. The results revealed the presence of Cronobacter spp. in a certain percentage of samples, mainly in nuts and mixes of nuts and dried fruits.
Article
Chemistry, Applied
Krzysztof Buksa, Magdalena Kowalczyk, Jakub Boreczek
Summary: This study found that the use of exopolysaccharides (EPS) produced by newly isolated lactic acid bacteria strains can limit the formation of high molecular weight resistant starch in wheat starch pastes. These EPS can be efficiently produced in sourdough, improving the properties of sourdough bread.
Article
Multidisciplinary Sciences
Dorota Litwinek, Jakub Boreczek, Halina Gambus, Krzysztof Buksa, Wiktor Berski, Magdalena Kowalczyk
Summary: Starter cultures composed of lactic acid bacteria (LAB) were developed for wholemeal rye bread production. The use of these cultures resulted in better quality bread compared to commercial culture. The addition of yeast during fermentation improved the organoleptic properties of the bread and increased its volume and moisture. Fermentation also improved the bioavailability of minerals.
Article
Microbiology
Przemyslaw Salanski, Magdalena Kowalczyk, Jacek K. Bardowski, Agnieszka K. Szczepankowska
Summary: This study explored the potential use of Lactococcus lactis strains in the treatment of gastrointestinal cancer. The results showed that these strains inhibited the proliferation of human colorectal adenocarcinoma cells and triggered gene expression in the cells. Additionally, the strains demonstrated probiotic potential, tolerating bile salts and acid exposure, and exhibiting adherence properties. Genetic analysis revealed functional and safety-related features of the strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Kosiorek, Anna Koryszewska-Baginska, Marek Skoneczny, Lidia Stasiak-Rozanska, Tamara Aleksandrzak-Piekarczyk
Summary: The L. lactis IL594 strain contains seven plasmids and is the parental strain of the plasmid-free L. lactis IL1403. Transcriptomic studies revealed that plasmidic genes could influence overall gene expression in industrially important L. lactis strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Kosiorek, Anna Koryszewska-Baginska, Marek Skoneczny, Tamara Aleksandrzak-Piekarczyk
Summary: This study found significant differences in the performance of L. lactis IL1403 without plasmids compared to L. lactis IL594 with plasmids. The presence of pIL2, pIL4, and pIL5 plasmids had the most pronounced effects on the metabolism of various carbon sources and the tolerance to antimicrobial compounds and heavy metal ions. Comparative transcriptomics revealed significant variations in the expression levels of multiple chromosomal genes due to the presence of single plasmids and the activity of all plasmids, suggesting both direct and indirect effects of plasmids on the observed phenotypic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Iryna Lushnikova, Olha Kostiuchenko, Magdalena Kowalczyk, Galyna Skibo
Summary: This mini-review focuses on the role of mTOR and aKG signaling in the metabolic homeostasis and neuroprotection of brain cells under oxygen-glucose deficiency conditions. Understanding the molecular events associated with cerebral ischemia and endogenous neuroprotection is crucial for enhancing therapeutic strategies.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Microbiology
Aleksandra Tymoszewska, Marlena Szylinska, Tamara Aleksandrzak-Piekarczyk
Summary: Pathogenic enterococci carrying virulence factors and resistance genes pose a significant risk in hospitals. Enterococcus faecium is a highly virulent and multidrug-resistant pathogen that requires urgent development of novel antimicrobial agents. In this study, the genetic basis of E. faecium's resistance to aureocin A53- and enterocin L50-like bacteriocins and antibiotics was investigated. Activation of the LiaFSR-mediated stress response was found to confer resistance to peptide antibiotics and bacteriocins by inducing cell envelope remodeling.
MICROBIOLOGY SPECTRUM
(2023)