Article
Chemistry, Multidisciplinary
Xueting Yang, Yue Ma, Xiuting Wang, Shengmeng Yuan, Fangjun Huo, Genzheng Yi, Jingyi Zhang, Bo Yang, Weidong Tian
Summary: This study successfully designed a 3D bioprinted biomimetic periodontal module using a specific cell-laden bioink, which showed favorable mechanical properties and orientation guidance. The module also provided a conducive biochemical environment for regulating cell behavior. The experiments conducted on animals demonstrated that the bioprinted module significantly enhanced the regeneration of periodontal tissues and opened up new possibilities for future clinical practice.
Review
Cell & Tissue Engineering
Xiuting Wang, Jinlong Chen, Weidong Tian
Summary: This article reviews the progress of stem cell-based tissue engineering and advanced strategies for endogenous regeneration in periodontal regeneration, including techniques such as utilizing cell aggregates to retain extracellular matrix and promoting regeneration through specific scaffold structures.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Yue Ma, Xueting Yang, Yan Chen, Jiumeng Zhang, Kuo Gai, Jinlong Chen, Fangjun Huo, Qiang Guo, Weihua Guo, Maling Gou, Bo Yang, Weidong Tian
Summary: This study reports the use of biomimetic peridontium patches (BPPs) for functional periodontal regeneration using a microscale continuous digital light projection bioprinting method. Orthotopic transplantation in the mandibles shows effective periodontal reconstruction, with the resulting bioengineered tissues closely resembling natural periodontium. Furthermore, the regenerative periodontium can achieve stable tooth movement under orthodontic migration force with no adverse consequences.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Jiayu Zhang, Tingting Lan, Xue Han, Yuchan Xu, Li Liao, Li Xie, Bo Yang, Weidong Tian, Weihua Guo
Summary: The study demonstrates that enhancing the antioxidative properties of ECM-based bioroots with NAC can protect seed cell viability and stemness against oxidative stress, resulting in improved transplant efficiency and better tissue regeneration. Transplantation experiments in rats show that NAC effectively preserves the biological properties of DFCs and facilitates bioroot regeneration by preventing replacement resorption or ankylosis.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Chao Liang, Li Liao, Weidong Tian
Summary: Decellularized extracellular matrix (dECM) promotes stem cell proliferation, migration, adhesion, and differentiation, making it a promising biomaterial for periodontal tissue engineering. It effectively preserves ECM components as they are in native tissue, providing ideal cues for regeneration and repair. Different sources of dECM have different advantages in promoting periodontal tissue regeneration. Various methods have been developed to improve the mechanical strength of dECM, such as functionalized scaffolds and crosslinked soluble dECM. dECM has been successful in periodontal regeneration therapies and holds potential for future applications.
Review
Dentistry, Oral Surgery & Medicine
Fabiana Divina Magalhaes, Giovanna Sarra, Giovanna Lopes Carvalho, Ana Clara Fagundes Pedroni, Marcia Martins Marques, Leandro Chambrone, Thais Gimenez, Maria Stella Moreira
Summary: The review of 33 studies indicates that cell sheets formed by mesenchymal stem cells derived from dental tissues have great potential for periodontal tissue regeneration in animal models, but the regeneration process is not always complete.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiuqun Wei, Shujuan Guo, Qian Liu, Li Liu, Fangjun Huo, Yafei Wu, Weidong Tian
Summary: Dental follicle stem cells (DFSCs) promote periodontal regeneration in an inflammatory microenvironment by expressing periostin and improving the immune microenvironment through macrophage recruitment and reprogramming. Silencing periostin attenuates the effects of DFSCs in promoting periodontal regeneration and regulating macrophages. Recombinant human periostin (rhPeriostin) can directly promote macrophage reprogramming and show potential for promoting periodontal regeneration when loaded in a collagen matrix.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Ju An Park, Yejin Youm, Hwa-Rim Lee, Yongwoo Lee, Sarah L. Barron, Taejeong Kwak, Gyu Tae Park, Young-Cheol Song, Roisin M. Owens, Jae Ho Kim, Sungjune Jung
Summary: A cell sheet transfer method from a culture surface to a target tissue is introduced in this study. UV-treated parylene, a commercially available flexible material, is used as the culture surface to provide adequate cell adhesiveness for both cell attachment during culture and efficient transfer. Various cell types are successfully transferred using this versatile cell-transfer process. The mechanism of interfacial cell migration between surfaces with different adhesion preferences is observed, and the re-establishment and reversal of cell polarity after transfer are identified. The unique capabilities of 3D multilayer stacking, freeform design, and curved surface application are demonstrated. The therapeutic potential of the cell-sheet delivery system is demonstrated through experiments on cutaneous wound healing and skin-tissue regeneration in mice models.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Xianling Gao, Meiliang Guan, Xuemin Liu, Hockin H. K. Xu, Qiting Huang, Lingling Chen, Shuheng Huang, Yin Xiao, Xuetao Shi, Zhengmei Lin
Summary: This study developed a simple and robust approach to the sustained release of bioactive molecules for endogenous periodontal regeneration. The PDD-ALN delivery system exhibits three synergistic effects on bone microenvironment, ultimately promoting periodontal regeneration in a rat model.
APPLIED MATERIALS TODAY
(2021)
Article
Cell & Tissue Engineering
Liya Ma, Nanquan Rao, Hui Jiang, Yuzhe Dai, Songtao Yang, Hefeng Yang, Jiangtian Hu
Summary: This study investigates the potential of DFSCs-sEVs in regenerating damaged periodontal tissue. The results show that DFSCs-sEVs promoted the proliferation, migration, and osteogenic differentiation of PDLSCs, potentially through the activation of the p38 MAPK signaling pathway. DFSCs-sEVs provide a novel strategy for periodontal regeneration.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Bin Luo, Yu Luo, Lin He, Yangyang Cao, Qingsong Jiang
Summary: This study investigated the influence of residual periodontal ligament on the dentin regeneration potential of dental pulp stem cells (DPSCs) in the jaw. Results showed that the presence of residual periodontal ligament promoted the regeneration of dentin-like tissue and increased the expression of dentin-specific proteins. RNA-seq and bioinformatic analysis revealed potential key factors and signaling pathways involved in the regeneration process.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Nanoscience & Nanotechnology
Yangheng Zhang, Peng Wang, Yuxian Wang, Jiao Li, Dan Qiao, Rixin Chen, Wenrong Yang, Fuhua Yan
Summary: The study showed that AuNPs can enhance osteogenesis of PDLSC sheets by promoting bone-related protein expression and mineralization, with 45-nm AuNPs being more effective than 13-nm ones. Additionally, AuNPs were found to activate the autophagy pathway to promote differentiation and enhance bone regeneration in ectopic models. These findings suggest a potential AuNP-based pretreatment strategy for improving CST in bone repair and regeneration.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Cell & Tissue Engineering
Huijiao Yan, Masamitsu Oshima, Resmi Raju, Swarnalakshmi Raman, Kazumitsu Sekine, Arief Waskitho, Miho Inoue, Masahisa Inoue, Otto Baba, Tsuyoshi Morita, Mayu Miyagi, Yoshizo Matsuka
Summary: This study aimed to investigate the regeneration of dentin-pulp complex using cell sheets, and found that the layered cell sheet group showed higher capability for hard tissue formation and could generate a structure similar to that of natural teeth.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Cell & Tissue Engineering
Genzheng Yi, Siyuan Zhang, Yue Ma, Xueting Yang, Fangjun Huo, Yan Chen, Bo Yang, Weidong Tian
Summary: This study found that matrix vesicles (MVs) can effectively improve the proliferation, migration, and osteogenic differentiation of dental follicle cells (DFCs), with collagenase-released MVs (CRMVs) showing better bone regeneration capabilities. In a rat alveolar bone defect model, CRMVs loaded on a collagen scaffold had more significant therapeutic outcomes compared to media MVs (MMVs) and DFCs-based treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Xun Xu, Li Liao, Weidong Tian
Summary: Prevascularization is an effective strategy to promote the survival and regeneration of grafts after transplantation, especially in repairing craniofacial tissue defects. The repair of craniofacial tissue defects is critical and transplantation of engineered tissues or biomaterials shows promising prospects. Revascularization plays a crucial role in ensuring the success of tissue regeneration.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Menghong Wang, Jie Li, Yanyan Ye, Duanjing Chen, Jinlin Song
Summary: This study found that exosomes from stem cells derived from human exfoliated deciduous teeth (SHED-exo) can improve proliferation, migration, cell cycle, apoptosis, and osteogenic differentiation of human periodontal ligament cells (hPDLCs). This highlights the therapeutic value of SHED-exo in the regeneration of periodontal tissues using hPDLCs in clinical practice.
Review
Dentistry, Oral Surgery & Medicine
Gang Liu, Xiaoning Qiu, Xinzhi Tan, Rong Miao, Weidong Tian, Wei Jing
Summary: This study assessed the efficacy of topical sialogogue spray containing 1% malic acid for treating xerostomia. The results showed that this spray significantly improved dry mouth symptoms and increased saliva flow rates. However, due to the limited number of studies, more research is needed in the future to provide higher quality evidence.
Article
Dentistry, Oral Surgery & Medicine
Yanli Huang, Li Liu, Qian Liu, Fangjun Huo, Xingyu Hu, Shujuan Guo, Weidong Tian
Summary: The study demonstrates that D-sEV and L-D-sEV can inhibit the pathogenicity of P. gingivalis and prevent the development of periodontitis.
Review
Multidisciplinary Sciences
Hanyue Li, Hongwei Dai, Jie Li
Summary: Mesenchymal stromal/stem cells (MSCs) have the potential to treat multiple inflammatory and immune diseases due to their easy acquisition and potent immuno-regulatory capacities. Recent studies show that MSC metabolism plays critical roles in immunomodulation, providing new insights for enhancing MSC-based therapy.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Cell Biology
Qian Liu, Shujuan Guo, Yanli Huang, Xiuqun Wei, Li Liu, Fangjun Huo, Ping Huang, Yafei Wu, Weidong Tian
Summary: Objective of this study was to explore the role and therapeutic function of TRPA1 in periodontitis. The study found that TRPA1 inhibitor significantly reduced oxidative and apoptotic levels in inflammatory cells, as well as reduced oxidative stress and apoptosis in periodontitis mice.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Engineering, Environmental
Bo Yang, Xueting Yang, Xiangyou Luo, Gang Chen, Jinlong Chen, Fangjun Huo, Zhuoli Zhu, Ye Tian, Weihua Guo, Weidong Tian
Summary: This study introduced a novel functional biological root with a sandwich structure, which successfully restored occlusal function and long-term masticatory function in rhesus monkeys. The chosen stem cells and scaffold materials displayed superior potential for bio-root construction, and the favorable regeneration potential of the biological root was proven in a non-human primate model.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Cell Biology
Jiawei Tang, Miao Tan, Siqi Liao, Mengwei Pang, Jie Li
Summary: This review summarizes the impact and mechanism of BMP-8a in female and male reproduction, teeth formation and eruption, bone and cartilage development, tissue differentiation, disease occurrence, progression, and prognosis. BMP-8a regulates the development of the uterus and plays a crucial role in late spermatogenesis. It is highly expressed in osteogenesis and differentially expressed in chondrogenesis. Furthermore, BMP-8a has a significant impact on the occurrence, development, and prognosis of various diseases.
CONNECTIVE TISSUE RESEARCH
(2023)
Article
Endocrinology & Metabolism
Hanyue Li, Yu Yuan, Hongying Chen, Hongwei Dai, Jie Li
Summary: This study investigated the effects and underlying mechanism of adipose-derived stromal/stem cell (ASC) therapy in experimental periodontitis. The results showed that ASCs can alleviate periodontitis by modulating macrophage polarization through the IDO-dependent Kyn-AhR-NRF2 pathway.
MOLECULAR METABOLISM
(2022)
Review
Food Science & Technology
Jiafei Tang, Xianglu Zhu, Anet Rezek Jambrak, Da-Wen Sun, Brijesh K. Tiwari
Summary: Compared with traditional methods, cavitation-based processing technology has attracted extensive attention for its low energy consumption and high processing efficiency. This review provides a detailed account of the cavitation mechanism, factors affecting cavitation, application in food processing, extraction of natural ingredients, and discusses safety, nutrition, and future research directions.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Liya Ma, Rui Chen, Yelin Zhang, Zichao Dai, Guobin Huang, Rongqiang Yang, Hefeng Yang
Summary: A tree shrew model of periodontitis was established and found to partially reproduce the pathological features of human periodontitis, providing theoretical support for using tree shrews as a potential model for human periodontitis.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Pingmeng Deng, Jing Huang, Qixuan Zhang, Yuejia Li, Jie Li
Summary: This study aims to investigate the role of EMILIN-1 in the osteo/odontogenic differentiation of hDPSCs. The results showed that EMILIN-1 plays a crucial role in the proliferation and osteo/odontogenic differentiation of hDPSCs. This finding provides new insights for teeth and bone tissue regeneration.
Article
Engineering, Biomedical
He Wang, Xiaowei Chang, Qian Ma, Boyang Sun, Han Li, Jinmin Zhou, Yiyao Hu, Xiaoyu Yang, Jie Li, Xin Chen, Jinlin Song
Summary: Researchers have designed a bioinspired drug-delivery system for diabetic periodontal bone regeneration. The system emulates the natural bone healing cascade by releasing drugs and recruiting stem cells to promote bone regeneration. In vitro and in vivo experiments have shown that the system is effective in restoring bone healing in diabetic patients.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Boyang Sun, Siqi Ying, Qian Ma, Han Li, Jie Li, Jinlin Song
Summary: Periodontitis is an oral chronic inflammatory disease with tissue destruction being inhibited and tissue regeneration being promoted as important means for its treatment. Metformin, with its hypoglycemic and anti-inflammatory effects, can inhibit oxidative stress and activate autophagy through the AMPK/mTOR pathway. This study explores the relationship between metformin and HMGB1 in the process of periodontitis, and its findings provide evidence for metformin as a potential drug for the treatment of periodontitis and HMGB1 as a potential target for periodontal intervention.
Article
Dentistry, Oral Surgery & Medicine
Qian Ma, Yiyao Hu, Han Li, Yunchun Kuang, Jie Li, Jinlin Song
Summary: In this study, it was found that DEL-1 promotes osteogenic differentiation of hPDLSCs in high glucose and inflammation environments, and it can rescue alveolar bone loss in ligature-induced experimental periodontitis with T2DM, providing a novel therapeutic target for periodontitis with T2DM.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
