Article
Behavioral Sciences
Jaquelinne Pinheiro-da-Silva, Ana Carolina Luchiari
Summary: Ethanol exposure during embryogenesis can have harmful effects on zebrafish, leading to reduced body size, decreased heartbeat rate, increased apoptotic cell numbers, and differences in hatching time. This study highlights the zebrafish as a suitable model for studying fetal alcohol spectrum disorders (FASD).
BRAIN AND BEHAVIOR
(2021)
Article
Environmental Sciences
Bala Murali Krishna Vasamsetti, Kyongmi Chon, Juyeong Kim, Jin-A Oh, Chang-Young Yoon, Hong-Hyun Park
Summary: This study investigated the effects of Thiram on the development of zebrafish embryos and found that Thiram is highly toxic, causing deformities and cardiac-related issues in zebrafish embryos.
Article
Biochemistry & Molecular Biology
Ivanildo Inacio da Silva Jr, Niely Priscila Correia da Silva, James A. Marrs, Pabyton Goncalves Cadena
Summary: This study evaluated the effects of lemongrass, thyme, and oregano essential oils on zebrafish embryos and larvae, and found that these oils can influence the development and cause morphological defects and behavioral changes in the embryos and larvae. Essential oil exposure may have neurodevelopmental toxicity on zebrafish. Therefore, careful use of essential oils, especially during pregnancy, should be considered.
Article
Cell Biology
Martin R. Silic, Ziyu Dong, Yueyi Chen, Adam Kimbrough, Guangjun Zhang
Summary: Research has revealed that zebrafish embryos exhibit specific cellular bioelectric signals at different stages of development, suggesting a potentially important role in zebrafish embryogenesis that could underlie human congenital diseases.
Article
Biochemistry & Molecular Biology
Francisco Pereira, Adriana Pereira, Sandra M. Monteiro, Carlos Venancio, Luis Felix
Summary: This study aimed to evaluate the antioxidant effects of a natural plant hormone, 24-epibrassinolide, against the teratogenic effects of nicotine on zebrafish embryos. The results showed that 24-epibrassinolide reduced nicotine-induced deformities in zebrafish embryos, while improving oxidative stress and metabolic changes. It also alleviated nicotine-induced cognitive dysfunction.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ana Carolina Seni-Silva, Adolfo Luis Almeida Maleski, Milena Marcolino Souza, Maria Alice Pimentel Falcao, Geonildo Rodrigo Disner, Monica Lopes-Ferreira, Carla Lima
Summary: This study evaluated the role of a family member of the Natterin protein family, LOC795232, in the embryonic development of zebrafish. The findings suggest that Natterin-like proteins potentially contribute to the embryonic development and immune response in zebrafish.
Article
Physiology
Ritika Ghosal, Gissela Borrego-Soto, Johann K. Eberhart
Summary: Forming a vertebrate head requires the integration of multiple tissue types, and prenatal alcohol exposure can cause various birth defects in the head. This study investigated the effects of ethanol on individual tissue types and their integration during craniofacial development. It was found that ethanol exposure induced ectopic cranial muscle and nerve defects, but only had slight effects on skeletal pattern. The defects in different tissues were independent of each other, and the effects of ethanol on the vertebrate head were highly pleiotropic.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Gaia Pucci, Giusi Irma Forte, Vincenzo Cavalieri
Summary: Radiotherapy is not yet personalized in cancer treatment plans, as its effectiveness varies depending on the radiosensitivity of tumor cells. Research is focusing on enhancing cancer cell response to ionizing radiation and protecting healthy tissues, using zebrafish as an effective model system for testing radiation modifiers for potential radiotherapeutic purposes in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Tarannum Taznin, Kaushalya Perera, Yann Gibert, Alister C. Ward, Clifford Liongue
Summary: This study investigated the expression and function of CRLF3, an evolutionarily significant protein, in early development, particularly in blood and immune cells, using zebrafish as a model. The study found that CRLF3 was expressed in multiple embryonic tissues and played a crucial role in primitive hematopoiesis and early definitive hematopoiesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gabriela Maressa Machado Baesso, Amanda Vitoria Venancio, Livia Costa Varela Barca, Pepita Fernanda Peppi, Caique Aparecido Faria, Joao Pedro Cosmo Machado, Luiz David Solis Murgas, Alvaro Jose de Almeida Bicudo, Eduardo Mauricio Mendes de Lima, Andre Rodrigues da Cunha Barreto-Vianna
Summary: This study evaluated the effects of eugenol, menthol, and lidocaine as anesthetics on glucose levels and recovery times in zebrafish. The results showed that eugenol and menthol did not affect glucose levels, while lidocaine caused non-concentration-dependent hyperglycemia. Recovery times were similar for eugenol and menthol, but concentration-dependent for lidocaine. Eugenol and menthol were deemed safe and satisfactory for glucose analysis in zebrafish research, while lidocaine could introduce biases and safety concerns. Researchers should carefully consider the selection of anesthetics for reliable results in zebrafish assessments.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Cell Biology
Chang Liu, Rui Li, Young Li, Xiumei Lin, Kaichen Zhao, Qun Liu, Shuowen Wang, Xueqian Yang, Xuyang Shi, Yuting Ma, Chenyu Pei, Hui Wang, Wendai Bao, Junhou Hui, Tao Yang, Zhicheng Xu, Tingting Lai, Michael Arman Berberoglu, Sunil Kumar Sahu, Miguel A. Esteban, Kailong Ma, Guangyi Fan, Yuxiang Li, Shiping Liu, Ao Chen, Xun Xu, Zhiqiang Dong, Longqi Liu
Summary: This study used Stereo-seq and scRNA-seq data to analyze the spatially resolved developmental trajectories of cell-fate transitions and molecular changes during zebrafish embryogenesis. Important interactions were identified, providing a fundamental reference for further studies on vertebrate development.
DEVELOPMENTAL CELL
(2022)
Article
Engineering, Environmental
Zulvikar Syambani Ulhaq, William Ka Fai Tse
Summary: In this study, zebrafish embryos were exposed to PFHxS and it was found that PFHxS accumulated in the embryos, leading to phenotypic abnormalities and disruption of cellular processes. Further analysis revealed that PFHxS affected glucose metabolism, lipid metabolism, and oxidative stress, resulting in increased reactive oxygen species, lipid accumulation, and cell cycle arrest. These findings highlight the potential toxicities of PFHxS.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Michael D. Nodine
Summary: Parental contributions affect early embryogenesis by regulating maternal resources distribution to offspring. A new study in Arabidopsis thaliana shows that signaling components from maternal sporophytic tissues and paternal gametes converge in zygotes to promote elongation of the extraembryonic suspensor, supporting embryo development.
Article
Fisheries
Heng-Chih Pan, Kang-Chieh Lo, Yun-Hsin Wang, Han-Ting Huang, Shu-Chun Cheng, Chiao-Yin Sun, Yau-Hung Chen
Summary: The results of this study indicate that klotho protein deficiency impairs cell movement during early fish development and affects gene expression.
Article
Biochemistry & Molecular Biology
Khadizatul Kubra, Gurveer K. Gaddu, Clifford Liongue, Somayyeh Heidary, Alister C. Ward, Amardeep S. Dhillon, Faiza Basheer
Summary: Members of the FOS protein family play important roles in regulating gene expression and are involved in pathological conditions. Using zebrafish as a model, this study found that zebrafish FOS orthologs exhibit distinct spatiotemporal expression patterns during embryonic development and in adult tissues, and are regulated by oncogenic signals. This suggests that zebrafish can serve as an alternate model for studying the regulation and functions of Fos proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Tania Martins, Paula Alexandra Oliveira, Maria Joao Pires, Maria Joao Neuparth, Germano Lanzarin, Luis Felix, Carlos Venancio, Maria de Lurdes Pinto, Joao Ferreira, Isabel Gaivao, Ana Isabel Barros, Eduardo Rosa, Luis Miguel Antunes
Summary: This pilot study evaluated the effects of broccoli by-product flour (BF) intake on the physiological parameters of mice. The results showed that BF supplementation reduced adipose tissue accumulation and had no toxic effects on the liver and kidney. BF supplementation also increased liver antioxidant response and enabled the bioavailability of beneficial glucosinolates and isothiocyanates.
Article
Environmental Sciences
Ondina Ribeiro, Luis Felix, Claudia Ribeiro, Bruno Castro, Maria Elizabeth Tiritan, Sandra Mariza Monteiro, Joao Soares Carrola
Summary: This study investigated the toxicity effects and possible enantioselective effects of venlafaxine on daphnia and zebrafish. The results showed that venlafaxine is not expected to induce harmful effects at environmental levels, but one enantiomer, (R)-VEN, increased malformations in zebrafish larvae.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Environmental Sciences
Luis Felix, Paulo Carreira, Francisco Peixoto
Summary: This study investigates the biological effects of naturally weathered microplastics (MPs) on zebrafish, revealing anxiety-like behavior and mitochondrial dysfunction caused by the MPs. It also emphasizes the potential cumulative impact of these particles on aquatic ecosystems.
Review
Fisheries
Luis Felix, Raquel Vieira, Sandra M. Monteiro, Carlos Venancio
Summary: The use of anaesthetic agents, including natural substances such as clove oil, as alternatives to the commonly used synthetic anaesthetic MS-222, has been investigated for fish anaesthesia. A meta-analysis of 30 English reports found that 10 different monoterpenes showed high effectiveness in inducing anaesthesia and recovery in fish. The findings also revealed an inverse linear association between anaesthesia induction and recovery time and water bath temperature.
FISH AND FISHERIES
(2023)
Article
Biochemistry & Molecular Biology
Francisco Pereira, Adriana Pereira, Sandra M. Monteiro, Carlos Venancio, Luis Felix
Summary: This study aimed to evaluate the antioxidant effects of a natural plant hormone, 24-epibrassinolide, against the teratogenic effects of nicotine on zebrafish embryos. The results showed that 24-epibrassinolide reduced nicotine-induced deformities in zebrafish embryos, while improving oxidative stress and metabolic changes. It also alleviated nicotine-induced cognitive dysfunction.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
Jose P. Castelao-Baptista, Sara A. Valente, Sara Canario, David Oppolzer, Ana Barros, Carlos Venancio, Tania Martins, Luis Antunes, Vilma A. Sardao, Eduardo Rosa, Paulo J. Oliveira
Summary: Obesity, a rising concern in the Eastern world, is often accompanied by non-alcoholic fatty liver disease (NAFLD). Researchers are investigating potential natural interventions to reduce obesity complications. This study focuses on Brassica by-products (BBPs) as an example of edible plant waste and examines the effects of different extracts from broccoli by-products on an in vitro model of fatty acid-induced liver toxicity. The extracts showed a dose-dependent decrease in cell viability of HepG2 cells, but did not compromise mitochondrial function or lipid accumulation. They effectively reduced lipid accumulation induced by fatty acids in HepG2 cells. The extracts' ability to prevent decrease in catalase activity may explain the observed effects.
Review
Environmental Sciences
Germano A. B. Lanzarin, Luis M. Felix, Antonio Fontainhas-Fernandes, Sandra Mariza Monteiro, Carlos Venancio
Summary: A critical review was conducted to investigate the toxicity of glyphosate and glyphosate-based herbicides on zebrafish. The results showed that both substances can cause damage to zebrafish, compromising their survival, reproduction, and maintenance. These findings may be applicable to other fish species and aquatic ecosystems.
Article
Agriculture, Dairy & Animal Science
Sara Jorge, Luis Felix, Benjamin Costas, Ana M. M. Valentim
Summary: Proper housing conditions are important for fish welfare. This study found that environmentally enriched housing conditions had minor effects on zebrafish behavior, but no significant influence on body length and stress hormone levels. It suggests that housing conditions should be considered in future research.
Article
Biochemistry & Molecular Biology
Germano A. B. Lanzarin, Luis M. Felix, Sandra M. Monteiro, Jorge M. Ferreira, Paula A. Oliveira, Carlos Venancio
Summary: This study investigated the anti-inflammatory, antioxidant, and anti-apoptotic effects of the plant-based products Thymol (THY) and 24-epibrassinolide (24-EPI). Using zebrafish larvae as a model, the researchers found that both compounds reduced neutrophil recruitment, exhibited antioxidant effects by reducing reactive oxygen species (ROS) production, and displayed anti-apoptotic effects. Furthermore, the compounds also decreased the levels of nitric oxide (NO). These findings suggest that THY and 24-EPI have potential as anti-inflammatory and antioxidant agents.
Article
Agriculture, Dairy & Animal Science
Mariana Caipira Lei, Luis Felix, Ricardo Cardoso, Sandra Mariza Monteiro, Severiano Silva, Carlos Venancio
Summary: This study investigates the use of saliva and eye infrared thermography as non-invasive methods to detect transport-induced stress in calves. The results show that transport increases salivary cortisol, oxidative stress parameters, and eye temperatures, indicating that saliva and infrared thermography are effective in assessing cows' stress status.
Article
Environmental Sciences
Ondina Ribeiro, Claudia Ribeiro, Luis Felix, Isabel Gaivao, Joao Soares Carrola
Summary: This study evaluated the potential effects of 3-MMC on zebrafish embryonic development, behavior, and DNA integrity. The results showed that at low concentrations, 3-MMC did not have expected harmful effects on critical development stages of fish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
Patricia Carneiro, Raquel Vieira, Carlos Venancio, Luis Felix
Summary: This study evaluated the effects of acute exposure to menthol on zebrafish embryos and found that it led to decreased hatching rate, heart rate, and increased malformations and mortality. At the molecular level, menthol exposure increased reactive oxygen species and antioxidant enzyme activity, as well as altered energy metabolism and mitochondrial function. At the behavioral level, menthol-treated larvae exhibited anxiety-like behavior. Additionally, there were decreases in dopaminergic neurons and acetylcholinesterase activity, indicating neuro-behavioral impairment.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Medicine, Research & Experimental
Tania Martins, Ruben Leite, Ana Filipa Matos, Joana Soares, Maria Joao Pires, Maria de lurdes Pinto, Maria Joao Neuparth, Ana Rita Sequeira, Luis Felix, Carlos Venancio, Sandra Mariza Monteiro, Bruno Colaco, Irene Gouvinhas, Ana Isabel Barros, Eduardo Rosa, Paula Alexandra Oliveira, Luis Miguel Antunes
Summary: This study found that broccoli by-products flour (BF) has anti-obesity effects in obese mice, helping to maintain lower body weight, reduce adipose tissue accumulation, and enhance the activity of antioxidant enzymes. Although there were no significant effects on liver weight, BF appears to be beneficial in preventing weight gain and fat accumulation induced by a high-fat diet.