期刊
CHEMBIOCHEM
卷 21, 期 3, 页码 331-334出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900435
关键词
affinity; amino-terminal copper and nickel (ATCUN) motif; copper; human serum albumin; metalloproteins
资金
- National Science Center (Poland) [2016/23/B/ST5/02253]
- Centre for Preclinical Research and Technology (CePT)
- European Regional Development Fund
- Innovative Economy, The National Cohesion Strategy of Poland
The apparent affinity of human serum albumin (HSA) for divalent copper has long been the subject of great interest, due to its presumed role as the major Cu2+-binding ligand in blood and cerebrospinal fluid. Using a combination of electronic absorption, circular dichroism and room-temperature electron paramagnetic resonance spectroscopies, together with potentiometric titrations, we competed the tripeptide GGH against HSA to reveal a conditional binding constant of log KCuCu(HSA)=13.02 +/- 0.05 at pH 7.4. This rigorously determined value of the Cu2+ affinity has important implications for understanding the extracellular distribution of copper.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据