Article
Endocrinology & Metabolism
Weirong Xing, Sheila Pourteymoor, Yian Chen, Subburaman Mohan
Summary: Studies show that targeted deletion of the Cldn12 gene in mice increases articular cartilage, potentially by promoting articular chondrocyte phenotype.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
Fan Zhang, Yuanyuan Wang, Ying Zhao, Manqi Wang, Bin Zhou, Xianpeng Ge, Di Chen
Summary: NFATc1 is a crucial regulator of articular chondrocyte differentiation, expressed in the early stage of joint development. It negatively regulates the transcriptional activity of the COL2A1 gene in articular cartilage progenitors, determining the differentiation of articular chondrocytes. These findings highlight the important role of NFATc1 in articular cartilage development.
Article
Endocrinology & Metabolism
Zelong Dou, Daniel Muder, Marta Baroncelli, Ameya Bendre, Alexandra Gkourogianni, Lars Ottosson, Torbjorn Vedung, Ola Nilsson
Summary: The study found that perichondrium and periosteum transplanted into articular cartilage defects can be transformed into cartilaginous articular surfaces. Perichondrium transplants developed into an articular-like, hyaline cartilage, while periosteum transplants produced a less resilient fibro-cartilage.
Article
Orthopedics
Kezhou Wu, Kar Wey Yong, Maha Ead, Mark Sommerfeldt, Tamara D. Skene-Arnold, Lindsey Westover, Kajsa Duke, Leila Laouar, Janet A. W. Elliott, Nadr M. Jomha
Summary: This study aimed to investigate whether the repair effect of vitrified particulated cartilage would be equivalent to or better than that of fresh cartilage. The experimental results showed that both fresh and vitrified particulated cartilage had equivalent regeneration within cartilage defects when evaluated at 6 months after surgery.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Developmental Biology
Azim Pothiawala, Berke E. Sahbazoglu, Bryan K. Ang, Nadine Matthias, Guangsheng Pei, Qing Yan, Brian R. Davis, Johnny Huard, Zhongming Zhao, Naoki Nakayama
Summary: It has been discovered that mesodermal progeny of human pluripotent stem cells can differentiate into two types of chondrogenic mesenchymal cells with distinct characteristics and functions. GDF5(+) cells can generate tissue similar to permanent cartilage, while SOX9(+) cells resemble neural crest-like progeny. These findings shed light on the cellular differentiation and molecular regulation mechanisms during cartilage development.
Article
Orthopedics
Shirish Pathak, Deepak Chaudhary, K. Raghuveer Reddy, Kiran K. Acharya, Sanjay M. Desai
Summary: The study assessed the mid-term efficacy and safety of gel-based autologous chondrocyte implantation (ACI) or autologous adult live cultured chondrocytes (CARTIGROW(R)) implantation in patients with cartilage defects of the knee joint. The results showed that both ACI and CARTIGROW(R) implantation significantly improved knee function and reduced pain in patients, with no reported adverse events.
INTERNATIONAL ORTHOPAEDICS
(2022)
Article
Orthopedics
Zheng Wang, Bin Liu, Kaifeng Lin, Chunguang Duan, Chunmei Wang
Summary: The presence and change of nerve fibers and neuropeptide during early development of articular cartilage in neonatal rats were investigated. The results showed that nerve fibers exist in the articular cartilage matrix and form synaptic contacts with chondrocytes. The neuropeptides SP and NPY significantly increase in articular cartilage during this period.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Po-Chih Shen, Cheng-Chang Lu, Shih-Hsiang Chou, Zi-Miao Liu, Shu-Jem Su, Yin-Chun Tien
Summary: This study compared stratified chondrocyte sheets (S-CS) and heterogeneous chondrocyte sheets (H-CS) in terms of expression and therapeutic effects, revealing that the S-CS group exhibited higher cell viability, proliferation rate, expression of chondrogenic markers, secretion of tissue inhibitor of metalloproteinase, and GAG production level. The mini-pigs articular cartilage defect model showed that the S-CS group had a higher International Cartilage Repair Society (ICRS) macroscopic score and displayed a zonal structure closer to native cartilage.
Review
Biochemistry & Molecular Biology
Darina Bacenkova, Marianna Trebunova, Jana Demeterova, Jozef Zivcak
Summary: Hyaline cartilage lacks self-repair potential, but tissue engineering methods can be used to address the issue of cartilage defect repair. Research shows that chondrocytes and mesenchymal stromal cells have favorable repair abilities in a three-dimensional culture environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Xiang Li, Shiao Li, Jiatian Qian, Yancheng Chen, Yiqin Zhou, Peiliang Fu
Summary: The study demonstrated that type I collagen-based MACT is an effective treatment for improving functionality and pain levels. Histological evidence confirmed hyaline cartilage induction and showed that repaired cartilage tended to emerge from the deep to the superficial layer. The quantitative MRI T2 mapping test indicated that there still was a difference between the transplanted cartilage and the surrounding hyaline cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Carlos Martinez-Armenta, Carlos Suarez-Ahedo, Anell Olivos-Meza, Maria C. Camacho-Rea, Laura E. Martinez-Gomez, Guadalupe Elizabeth Jimenez-Gutierrez, Gabriela A. Martinez-Nava, Luis E. Gomez-Quiroz, Carlos Pineda, Alberto Lopez-Reyes
Summary: In this study, a scaffold-free 3D culture system was developed to generate spheroids from human chondrocytes. The results showed that spheroids developed in a hypoxic environment exhibited better chondrogenesis characteristics compared to those developed under normoxic conditions. This novel culture system could be a valuable tool for cartilage biology research.
Article
Biochemistry & Molecular Biology
Susumu Takemura, Yoichi Ohta, Ryo Sugama, Yukihide Minoda, Yohei Ohyama, Hiroaki Nakamura
Summary: This study found that eldecalcitol can upregulate articular cartilage markers in superficial zone chondrocytes (SZC) and suppress differentiation markers in deep zone chondrocytes (DZC). This regulation may have potential efficacy in inhibiting the progression of osteoarthritis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Kai Qiao, Qi Chen, Yiguo Cao, Jie Li, Gang Xu, Jiaqing Liu, Xiaolin Cui, Kang Tian, Weiguo Zhang
Summary: Cartilage lesions and degenerative joint diseases are two leading contributors to global disability, with current cell-based therapies facing limitations such as high tumorigenicity risk and low retention rate. Non-stem cell-based therapy, particularly extracellular vesicles (EVs), have gained popularity as they have been shown to achieve therapeutic effects similar to stem cells in cartilage tissue engineering. Further research on the therapeutic role of EVs in these conditions is warranted, given their potential to offer effective treatment options in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Medicine, Research & Experimental
Weichang Xu, Jing Zhu, Jiawei Hu, Lin Xiao
Summary: Tissue engineering holds great promise in cartilage injury repair and replacement. However, issues such as immunogenicity, stability, and mechanical strength have remained unsolved in articular cartilage tissue engineering. The lack of replicating the chondrocyte biomechanical microenvironment (BME) has been a notable factor. Recent studies have highlighted the crucial role of BME in chondrocyte phenotype and cartilage functions, leading to more precise research in engineering the chondrocyte BME for articular cartilage tissue engineering. This review discusses the effects of the chondrocyte BME and explores the strategies for engineering it in articular cartilage tissue engineering, with a focus on simulating key characteristics and providing dynamic mechanical stimulation.
Article
Biochemistry & Molecular Biology
Masato Kobayashi, Ryota Chijimatsu, Hideki Yoshikawa, Kiyoshi Yoshida
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Cell & Tissue Engineering
Junya Higuchi, Ryota Yamagami, Takumi Matsumoto, Tomohiro Terao, Keita Inoue, Shinsaku Tsuji, Yuji Maenohara, Tokio Matsuzaki, Ryota Chijimatsu, Yasunori Omata, Fumiko Yano, Sakae Tanaka, Taku Saito
REGENERATIVE THERAPY
(2020)
Article
Multidisciplinary Sciences
Junichi Kushioka, Takashi Kaito, Ryota Chijimatsu, Rintaro Okada, Hiroyuki Ishiguro, Zeynep Bal, Joe Kodama, Fumiko Yano, Taku Saito, Ung-il Chung, Sakae Tanaka, Hideki Yoshikawa
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Gensuke Okamura, Kosuke Ebina, Makoto Hirao, Ryota Chijimatsu, Yasukazu Yonetani, Yuki Etani, Akira Miyama, Kenji Takami, Atsushi Goshima, Hideki Yoshikawa, Takuya Ishimoto, Takayoshi Nakano, Masayuki Hamada, Takashi Kanamoto, Ken Nakata
Summary: Adding basic fibroblast growth factor (bFGF) to the culture of synovial mesenchymal stem cells (SMSC) can significantly promote cartilage regeneration in vivo, leading to better cartilage repair outcomes and more obvious cartilage matrix formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Yuji Maenohara, Ryota Chijimatsu, Naohiro Tachibana, Kosuke Uehara, Fengjun Xuan, Daisuke Mori, Yasutaka Murahashi, Hideki Nakamoto, Takeshi Oichi, Song Ho Chang, Takumi Matsumoto, Yasunori Omata, Fumiko Yano, Sakae Tanaka, Taku Saito
Summary: Loss of function of the proteoglycan 4 (Prg4) gene leads to increased articular cartilage thickness and disappearance of SFZ cells, with upregulation of Mmp9 and phosphorylated Smad2 expression. These findings highlight the importance of lubricin in maintaining articular cartilage homeostasis and suggest the involvement of the NF-κB-Mmp9-TGF-β pathway in the downstream effects of lubricin.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Masato Kobayashi, Ryota Chijimatsu, David A. Hart, Shuichi Hamamoto, George Jacob, Fumiko Yano, Taku Saito, Kazunori Shimomura, Wataru Ando, Ung-il Chung, Sakae Tanaka, Hideki Yoshikawa, Norimasa Nakamura
Summary: This study investigated the effect of TD-198946 pretreatment on hSSCs and found that TD-198946 enhances the chondrogenic potential of hSSCs through the NOTCH3 signaling pathway. The activation of NOTCH3 by TD-198946 provides a novel insight into regulating the differentiation of hSSCs into chondrocytes.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Clinical Neurology
Zeynep Bal, Feza Korkusuz, Hiroyuki Ishiguro, Rintaro Okada, Junichi Kushioka, Ryota Chijimatsu, Joe Kodama, Daisuke Tateiwa, Yuichiro Ukon, Shinichi Nakagawa, Eda Ciftci Dede, Merve Gizer, Petek Korkusuz, Hideki Yoshikawa, Takashi Kaito
Summary: The novel composite material of nHAp/PLA-PEG demonstrated efficient bone regeneration with low-dose BMP-2. ELISA results showed sustained release of BMP-2 up to day 21, with 91.6% of specimens in both the low- and high-dose groups achieving solid fusion according to μCT and manual palpation test, while none in the control group achieved bony fusion.
Article
Rheumatology
Hideki Nakamoto, Yuki Katanosaka, Ryota Chijimatsu, Daisuke Mori, Fengjun Xuan, Fumiko Yano, Yasunori Omata, Yuji Maenohara, Yasutaka Murahashi, Kohei Kawaguchi, Ryota Yamagami, Hiroshi Inui, Shuji Taketomi, Yuki Taniguchi, Motoi Kanagawa, Keiji Naruse, Sakae Tanaka, Taku Saito
Summary: This study reveals the regulation of articular cartilage by TRPV2 through Prg4 induction and suppression of ectopic ossification.
ARTHRITIS & RHEUMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Masatoshi Kitakaze, Ryota Chijimatsu, Andrea Vecchione, Toru Kitagawa, Yuichiro Doki, Hidetoshi Eguchi, Hideshi Ishii
Summary: Recent advances in deciphering the human genome have allowed a better understanding of age-associated transformations, including gene functional interactions and the impact of single cell mechanisms on aging and disease. Genomic age is defined by a combination of inherited and acquired genetic traits, influenced by internal and external factors, leading to diseases such as cancer and cell degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yu Takeda, Ryota Chijimatsu, Andrea Vecchione, Takahiro Arai, Toru Kitagawa, Ken Ofusa, Masami Yabumoto, Takaaki Hirotsu, Hidetoshi Eguchi, Yuichiro Doki, Hideshi Ishii
Summary: The article highlights the importance of one-carbon metabolism in cancer research related to the folate cycle, discussing recent advances in understanding cellular biochemical reactions and reactive oxygen species in cancer cells. Further research is needed to explore therapeutic drug discovery and develop precision cancer medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Joe Kodama, Anjar Anggraini Harumningtyas, Tomoko Ito, Miroslav Michlicek, Satoshi Sugimoto, Hidekazu Kita, Ryota Chijimatsu, Yuichiro Ukon, Junichi Kushioka, Rintaro Okada, Takashi Kamatani, Kunihiko Hashimoto, Daisuke Tateiwa, Hiroyuki Tsukazaki, Shinichi Nakagawa, Shota Takenaka, Takahiro Makino, Yusuke Sakai, David Necas, Lenka Zajickova, Satoshi Hamaguchi, Takashi Kaito
Summary: Surface modification of artificial bone using low-pressure plasma technology can enhance hydrophilicity and promote cell infiltration, adhesion, and osteogenic differentiation, leading to high bone regeneration capacity in animal models. This represents a promising strategy for overcoming current clinical limitations in the use of artificial bone.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Chijimatsu Ryota, Miwa Satoshi, Okamura Gensuke, Miyahara Junya, Tachibana Naohiro, Ishikura Hisatoshi, Higuchi Junya, Maenohara Yuji, Tsuji Shinsaku, Sameshima Shin, Takagi Kentaro, Nakazato Keiu, Kawaguchi Kohei, Yamagami Ryota, Inui Hiroshi, Taketomi Shuji, Sakae Tanaka, Taku Saito
Summary: This study investigated the chondrogenic potential of stem cells derived from adipose tissue and synovium, showing intrinsic differences in chondrogenic factors among cell types and species. The response to TGF beta 1 may play a crucial role in determining the fate of stem cells transplanted into cartilage defects.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Endocrinology & Metabolism
Taizo Kaneko, Keisuke Horiuchi, Ryota Chijimatsu, Daisuke Mori, Kosei Nagata, Yasunori Omata, Fumiko Yano, Hiroshi Inui, Toru Moro, Sakae Tanaka, Taku Saito
Summary: The study demonstrated an increased expression of Adam17 in mouse articular cartilage during OA progression, and showed that Adam17 knockout mice exhibited attenuated cartilage degradation progression, indicating involvement in EGFR and TNF pathways. Activation of Adam17 increased release of Mmp13 and decreased aggrecan, while knockout had the opposite effect, suggesting Adam17 as a potential therapeutic target for OA.
JOURNAL OF BONE AND MINERAL METABOLISM
(2022)
Review
Oncology
Shotaro Tatekawa, Ken Ofusa, Ryota Chijimatsu, Andrea Vecchione, Keisuke Tamari, Kazuhiko Ogawa, Hideshi Ishii
Summary: Cancer metabolism plays a crucial role in the tumor microenvironment by defining a biologically malignant phenotype through methylation. Targeting the methylosystem may be an effective therapeutic approach against refractory cancers.
Article
Cell & Tissue Engineering
Junichi Kushioka, Takashi Kaito, Rintaro Okada, Hiroyuki Ishiguro, Zeynep Bal, Joe Kodama, Ryota Chijimatsu, Melanie Pye, Masahiro Narimatsu, Jeffrey L. Wrana, Yasumichi Inoue, Hiroko Ninomiya, Shin Yamamoto, Takashi Saitou, Hideki Yoshikawa, Takeshi Imamura
