期刊
BMC PLANT BIOLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12870-019-1872-1
关键词
Abscisic acid; Drought tolerance; OsbZIP62; Oxidative tolerance; Rice; Transcription factor
资金
- Shanghai Agriculture Applied Technology Development Program [2017-02-00-08-F00071]
- Shared Platform of Crop Germplasm Resources in Shanghai [18DZ2293700]
- Shanghai Natural Sciences Foundation [16ZR1431200]
- Shanghai Agricultural Academy of Science [2017(A-01)]
BackgroundDrought is a major abiotic stress factor that influences the yield of crops. Basic leucine zipper motif (bZIP) transcription factors play an important regulatory role in plant drought stress responses. However, the functions of a number of bZIP transcription factors in rice are still unknown.ResultsIn this study, a novel drought stress-related bZIP transcription factor, OsbZIP62, was identified in rice. This gene was selected from a transcriptome analysis of several typical rice varieties with different drought tolerances. OsbZIP62 expression was induced by drought, hydrogen peroxide, and abscisic acid (ABA) treatment. Overexpression of OsbZIP62-VP64 (OsbZIP62V) enhanced the drought tolerance and oxidative stress tolerance of transgenic rice, while osbzip62 mutants exhibited the opposite phenotype. OsbZIP62-GFP was localized to the nucleus, and the N-terminal sequence (amino acids 1-68) was necessary for the transcriptional activation activity of OsbZIP62. RNA-seq analysis showed that the expression of many stress-related genes (e.g., OsGL1, OsNAC10, and DSM2) was upregulated in OsbZIP62V plants. Moreover, OsbZIP62 could bind to the promoters of several putative target genes and could interact with stress/ABA-activated protein kinases (SAPKs).ConclusionsOsbZIP62 is involved in ABA signalling pathways and positively regulates rice drought tolerance by regulating the expression of genes associated with stress, and this gene could be used for the genetic modification of crops with improved drought tolerance.
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