Article
Food Science & Technology
Urszula Zarzecka, Arkadiusz Jozef Zakrzewski, Wioleta Chajecka-Wierzchowska, Anna Zadernowska
Summary: This study aimed to define the genetic mechanisms of linezolid resistance in enterococci isolated from animal origin foods. The results showed that 26.9% of isolates displayed linezolid resistance, with the most common acquired resistance gene being poxtA. The presence of mobile genetic elements encoding this resistance poses a rapid transmission risk.
Article
Infectious Diseases
Loren Dejoies, Mohamed Sassi, Sacha Schutz, Julie Moreaux, Asma Zouari, Sophie Potrel, Anais Collet, Maxime Lecourt, Gabriel Auger, Vincent Cattoir
Summary: The study revealed the prevalence of poxtA gene in clinical enterococci in France since 2016, mostly in E. faecium and independently from optrA. Plasmids carrying poxtA also often co-carried resistance genes to phenicols and tetracyclines, potentially co-selected through veterinary use.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Food Science & Technology
Ihab Habib, Akela Ghazawi, Glindya Bhagya Lakshmi, Mohamed-Yousif Ibrahim Mohamed, Dan Li, Mushtaq Khan, Shafi Sahibzada
Summary: The transfer of resistant genes from enterococci to humans through food and their tolerance to commonly used antimicrobials is a growing concern globally. This study characterized the first reported isolates of linezolid-resistant enterococci carrying the optrA gene from supermarket broiler meat in the UAE. The isolates showed multidrug resistance and various virulence traits. Further monitoring of linezolid resistance is crucial, and enterococci can serve as an indicator for antimicrobial resistance spread at the human-food interface.
Article
Infectious Diseases
Qiaojun Wang, Kai Peng, Ziyi Liu, Yan Li, Xia Xiao, Xiang-Dang Du, Ruichao Li, Zhiqiang Wang
Summary: This study aimed to determine the molecular mechanisms of linezolid-resistant enterococci (LRE) in swine slaughterhouses in China and analyze the evolutionary dynamics of poxtA-positive E. faecium worldwide. Swine faeces were found to be a significant reservoir for LRE, and optrA and poxtA were the main contributors to linezolid resistance. Phylogenetic analysis revealed the potential global transmission of CC17 poxtA-positive E. faecium between humans and animals.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Paula Gagetti, Diego Faccone, Paola Ceriana, Celeste Lucero, Alejandra Menocal, Grupo L. R. E. Argentina, Alejandra Corso
Summary: This study characterized 14 optrA-carrying linezolid resistant E. faecalis isolates recovered from seven hospitals in Argentina between 2016 and 2021. The isolates were multidrug-resistant and carried multiple antimicrobial resistance genes. The optrA variants and lineages varied among the isolates.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Sonia Nina Coccitto, Marzia Cinthi, Serena Simoni, Carla Vignaroli, Francesca Romana Massacci, Elisa Albini, Cristiana Garofalo, Lucia Aquilanti, Chiara Francesca Magistrali, Andrea Brenciani, Eleonora Giovanetti
Summary: This study found that enterococci from food-producing animals carry resistance genes that pose a serious threat to human health. The genes, including optrA and cfr(D), were found on transferable plasmids, which could potentially spread to human pathogens.
VETERINARY MICROBIOLOGY
(2023)
Article
Infectious Diseases
Yeong Bin Kim, Sunghyun Yoon, Kwang Won Seo, Jong Bo Shim, Eun Bi Noh, Young Ju Lee
Summary: This study investigated the presence and distribution of Linezolid-resistant Enterococcus faecalis and Enterococcus faecium in a layer operation system, revealing a high prevalence of resistant strains with multidrug resistance. The optrA gene was found to be widely distributed in E. faecalis, suggesting a high level of transferability. Enhanced surveillance efforts are needed to control the spread of optrA in enterococci in layer operation system.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Microbiology
Yizhi Tang, Yan Lai, Xingyuan Wang, Changwei Lei, Chao Li, Linghan Kong, Yulong Wang, Hongning Wang
Summary: A novel transposon ISChh1-like was identified in Campylobacter, mediating acquisition of multiple antibiotic resistance genes, including the important optrA gene.
VETERINARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eiseul Kim, So-Won Shin, Hyo-Sun Kwak, Min-Hyeok Cha, Seung-Min Yang, Yoon-Soo Gwak, Gun-Jo Woo, Hae-Yeong Kim
Summary: The prevalence and characteristics of phenicol-oxazolidinone resistance genes in Enterococcus faecalis and Enterococcus faecium isolated from food-producing animals and meat in Korea were analyzed in 2018. The study found that these resistance genes are commonly present on both the chromosome and plasmid, and can facilitate horizontal transfer with other antimicrobial-resistant genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Yamuna Devi Bakthavatchalam, Karthick Vasudevan, Priyanka Babu, Ayyan Raj Neeravi, Vignesh Narasiman, Balaji Veeraraghavan
Summary: This study reports optrA-positive linezolid-resistant E. faecium isolates from India for the first time, highlighting the potential threat for dissemination with a novel plasmid co-carrying vancomycin and linezolid resistance determinants.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Lucia Malisova, Vladislav Jakubu, Katarina Pomorska, Martin Musilek, Helena Zemlickova
Summary: The main mechanisms of linezolid resistance in linezolid-resistant enterococci in the Czech Republic were found to be the G2576T mutation in the domain V of 23S rRNA and the presence of the optrA gene, with some isolates also showing resistance to tigecycline and vancomycin.
Article
Infectious Diseases
Yuxin Hu, Dongju Won, Le Phuong Nguyen, Kennedy Mensah Osei, Younghee Seo, Junglim Kim, Yoonhee Lee, Hyukmin Lee, Dongeun Yong, Jong Rak Choi, Kyungwon Lee
Summary: This study analyzed linezolid-nonsusceptible enterococci (LNSE) strains isolated from a tertiary care hospital, revealing that the primary resistance mechanism was the optrA gene, and the co-existence of the cfr(D) and poxtA2 genes also contributed to resistance. The study also identified a G2576U mutation on 23S rRNA in E. faecium, which significantly increased linezolid MIC.
Article
Microbiology
Zheren Huang, Yilin Bai, Qin Wang, Xue Yang, Tiejun Zhang, Xuan Chen, Hongning Wang
Summary: This study identifies the presence of transferable oxazolidinone resistance genes in enterococcal isolates from different production stages in a pig farm in China. The dissemination of these genes poses a major challenge to public health and environmental safety, emphasizing the need for continuous monitoring and research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Wenlin Yang, Qiu Xu, Qin Yang, Lingli Wang, Siguo Liu, Yao Zhu, Wanjiang Zhang
Summary: The occurrence and dissemination of linezolid-resistant Gram-positive bacteria among food-producing animals poses severe threats to public health. In this study, the optrA resistance gene was found in a multi-drug resistant strain of E.faecalis isolated from a goat in China. The strain belonged to the high-risk clone ST16 and carried the optrA gene on a novel plasmid. Active surveillance of optrA-positive E.faecalis high-risk clones in food-producing animals is urgently warranted.
VETERINARY MICROBIOLOGY
(2022)
Article
Infectious Diseases
Joana Giao, Celia Leao, Teresa Albuquerque, Lurdes Clemente, Ana Amaro
Summary: This study investigates the prevalence and diversity of enterococcal species in the cecal samples of cattle and pigs. The researchers found a wide range of enterococci colonizing the intestines of these animals. Although resistance to critically important antibiotics was generally low, erythromycin resistance was an exception. The study also identified daptomycin-resistant and linezolid-resistant strains, as well as oxazolidinone resistance determinants in enterococci.