Review
Immunology
Odir Antonio Dellagostin, Sibele Borsuk, Thais Larre Oliveira, Fabiana Kommling Seixas
Summary: This review explores the strategies for developing and using auxotrophic BCG strains, which can serve as a model for studying M. tuberculosis and potentially provide a stable and effective alternative to BCG, as well as a vector for recombinant live vaccines.
Review
Infectious Diseases
Christoph Lange, Peter Aaby, Marcel A. Behr, Peter R. Donald, Stefan H. E. Kaufmann, Mihai G. Netea, Anna M. Mandalakas
Summary: The BCG vaccine, initially designed for cattle, has saved many lives since its first administration to a newborn in Paris in 1921. While providing strong protection against miliary and meningeal tuberculosis in children, it is less effective in preventing pulmonary tuberculosis in adults. It has also shown non-specific benefits against non-tuberculous infections and immunotherapeutic benefits in certain cancers.
LANCET INFECTIOUS DISEASES
(2022)
Article
Immunology
Nicola Campbell, Ayesha J. Verrall, Simon Donkor, Jayne S. Sutherland, Philip C. Hill
Summary: The study found that BCG vaccine protection against Mycobacterium tuberculosis infection decreased with increasing exposure to the pathogen in the Gambia, with the strongest protection observed in the lowest exposure tertile. These findings are consistent with previous results from Indonesia.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Wei Lv, Pu He, Yanlin Ma, Daquan Tan, Fei Li, Tao Xie, Jiangyuan Han, Juan Wang, Youjun Mi, Hongxia Niu, Bingdong Zhu
Summary: Optimizing the boosting schedule of subunit vaccines can enhance long-term protection against tuberculosis. Boosting with MH twice or immunizing with EC thrice after BCG priming can improve immune memory and increase protection against bacteria.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Patricio Retamal, Pedro abalos, Raul Alegria-Moran, Nicolas Valdivieso, Martin Vordermeier, Gareth Jones, Karina Saadi, Carolina Perez Watt, Constanza Salinas, Constanza avila, Valentina Padilla, Belen Benavides, Romina Orellana
Summary: The study evaluated the efficacy of vaccinating dairy cattle with the BCG Russia strain in a high prevalence area of Chile for tuberculosis prevention. Results showed a significant reduction in infection rates and beneficial effects on milk production parameters.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
C. Kanipe, P. M. Boggiatto, E. J. Putz, M. V. Palmer
Summary: The study found that BCG vaccination can reduce the number and size of tuberculosis lesions, decrease necrosis, and increase fibrosis in low-grade lesions. Vaccination has minimal impact on mineralization and density of multinucleated giant cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Viridiana Garcia-Ruiz, Patricia Orduna, Antonia Castillo-Rodal, Teresa J. Flores-Rodriguez, Yolanda Lopez-Vidal
Summary: This study aimed to investigate the differences in glycosylation profiles between two recombinant strains of Mycobacterium microti and found distinct proteome and glycoproteome profiles between the strains.
Article
Immunology
Rania Bouzeyen, Saurabh Chugh, Tannu Priya Gosain, Mohamed-Ridha Barbouche, Meriam Haoues, Kanury V. S. Rao, Makram Essafi, Ramandeep Singh
Summary: This study demonstrates that enhancing the protective efficacy of M. bovis BCG in mice and guinea pig infection models by inhibiting Akt inhibitor MK-2206 promotes FOXO3 activation and BCG-induced immune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Eamonn Gormley, Deirdre Ni Bhuachalla, Tara Fitzsimons, James O'Keeffe, Guy McGrath, Jamie M. Madden, Naomi Fogarty, Kevin Kenny, Locksley L. Mc Messam, Denise Murphy, Leigh A. L. Corner
Summary: Vaccination of badgers with Mycobacterium bovis Bacille Calmette-Guerin (BCG) has been shown to protect badgers against tuberculosis in experimental trials. The study results suggest that BCG vaccination could indirectly protect unvaccinated badgers, leading to a high level of population immunity against tuberculosis. This indicates that BCG vaccination could be an effective method for reducing tuberculosis incidence in badger populations.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Lazaro Moreira Marques-Neto, Monalisa Martins Trentini, Alex Issamu Kanno, Dunia Rodriguez, Luciana Cezar de Cerqueira Leite
Summary: The rBCG-LTAK63 vaccine induces a long-term polyfunctional Th1/Th17 response and an increased presence of memory T cells, leading to improved protection against Mycobacterium tuberculosis (Mtb) in mice. This enhanced immune response may contribute to the long-term protection provided by the vaccine.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Mitchell Foster, Philip C. Hill, Todia Pediatama Setiabudiawan, Valerie A. C. M. Koeken, Bachti Alisjahbana, Reinout van Crevel
Summary: This review examines the protective effect of BCG vaccine against Mtb infection, discusses limitations and variations, and explores potential mechanisms for BCG efficacy against Mtb infection, including the role of trained immunity.
IMMUNOLOGICAL REVIEWS
(2021)
Review
Microbiology
Nonna I. Nadolinskaia, Maria S. Kotliarova, Anna V. Goncharenko
Summary: Tuberculosis is a major infectious disease with high mortality rates in certain regions, and the existing BCG vaccine is ineffective for long-term protection. The exact immune factors crucial for preventing Mycobacterium tuberculosis infection and progression of the disease are still unknown after decades of research. Developing a new vaccine to replace BCG and provide better protection is a challenging task due to the intracellular lifestyle of the pathogen and its manipulation of host immunity. This review discusses promising strategies and possibilities for creating a new vaccine, including supplementing mycobacterial strains with immunodominant antigens and genetic engineering to alter the bacterium-host cell interaction.
Article
Biotechnology & Applied Microbiology
Saba Savul, Susan Duthie
Summary: This study reviewed the BCG vaccination records from 2019 to 2021 in Grampian, Scotland and found a high uptake rate, surpassing the key performance indicator target in Scotland and showing improvement compared to earlier rates. Strengthening electronic systems and raising awareness can further enhance BCG vaccination uptake.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Immunology
Ana Paula Junqueira-Kipnis, Carine de Castro Souza, Ana Carolina de Oliveira Carvalho, Fabio Muniz de Oliveira, Vinnycius Pereira Almeida, Alisson Rodrigues de Paula, Mara Rubia Celes, Andre Kipnis
Summary: A new tuberculosis vaccine PEPf was developed, which can reduce lung bacterial load and improve protection. Combining PEPf with the Advax4 adjuvant and using a prime-boost strategy as a subunit vaccine significantly enhances protection.
Article
Microbiology
Fabiola Silva, Raphael Enaud, Elizabeth Creissen, Marcela Henao-Tamayo, Laurence Delhaes, Angelo Izzo
Summary: This study aimed to investigate the effects of BCG vaccination and M. tuberculosis infection on the gut and lung microbiota in mice. The results showed that both vaccination and infection had an impact on the relative abundance and diversity of microbiota in the lung and gut. However, the lung was more affected than the gut. The study also revealed that the host's local response to infection affected the overall microbial flora, while the immune response to vaccination mainly influenced the gut microbiota. This study highlights the importance of gut and lung microbiota in the immunopathogenesis of tuberculosis and suggests that manipulating these microbial communities could improve vaccine response and develop treatment adjuvants.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Preeti Sahay, Kshama Jain, Prakriti Sinha, Barun Das, Alaknanda Mishra, Ashwani Kesarwani, Parul Sahu, K. Varsha Mohan, M. J. Mahesh Kumar, Perumal Nagarajan, Pramod Upadhyay
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Immunology
Vivek Ranjan, Alaknanda Mishra, Ashwani Kesarwani, K. Varsha Mohan, Sandeep Narayan Lal, Jacob Puliyel, Pramod Upadhyay
Article
Biochemistry & Molecular Biology
Parul Sahu, K. Varsha Mohan, Savera Aggarwal, Shailendra Arindkar, Jerald Mahesh Kumar, Pramod Kumar Upadhyay, Gayatri Ramakrishna, Perumal Nagarajan
Summary: The study revealed that mice with AIF deficiency did not develop liver injury on high fat high fructose diet and after bile duct ligation, indicating a potential hepatoprotective role of the AIF-mediated pathway.
CELL BIOCHEMISTRY AND FUNCTION
(2021)
Article
Critical Care Medicine
Kushagra Khanna, Ritu Karwasra, Sandeep Choudhary, Deeksha Sharma, Nitin Sharma, Puja Nagpal, Varsha Chorsiya, Dhruv Kumar Nishad, Harvinder Popli, Aseem Bhatnagar
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2020)
Article
Cell & Tissue Engineering
Alaknanda Mishra, K. Varsha Mohan, Perumal Nagarajan, Srikanth Iyer, Ashwani Kesarwani, Madhu Nath, Laxmi Moksha, Jashdeep Bhattacharjee, Barun Das, Kshama Jain, Parul Sahu, Prakriti Sinha, T. Velapandian, Pramod Upadhyay
STEM CELL RESEARCH & THERAPY
(2020)
Article
Multidisciplinary Sciences
Ashwani Kesarwani, Parul Sahu, Kshama Jain, Prakriti Sinha, K. Varsha Mohan, Puja S. Nagpal, Surender Singh, Rana Zaidi, Perumal Nagarajan, Pramod Upadhyay
Summary: The limitations of BCG vaccine for tuberculosis necessitate the development of a more effective and safe alternative. The evaluation of BEAP in infant rhesus macaques demonstrated its non-toxicity and ability to activate the immune system to eliminate infected macrophages, suggesting its safety and efficacy as a potential vaccine option.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Francois Cherbonneau, Guoping Li, Priyanka Gokulnath, Parul Sahu, Aurore Prunevieille, Robert Kitchen, Gilles Benichou, Jerome Larghero, Ibrahim Domian, Saumya Das
Summary: Current methods for analyzing gene expression require cellular destruction, making continuous analysis difficult. However, our recently developed TRACE-seq technology allows transcriptomic analysis without cell lysis, providing accurate data on stress-responsive transcriptomic changes.
Article
Engineering, Biomedical
Howard H. Chen, Zehedina Khatun, Lan Wei, Choukri Mekkaoui, Dakshesh Patel, Sally Ji Who Kim, Asma Boukhalfa, Efosa Enoma, Lin Meng, Yinching Chen, Leena Kaikkonen, Guoping Li, Diane E. Capen, Parul Sahu, Anand T. N. Kumar, Robert M. Blanton, Hushan Yuan, Saumya Das, Lee Josephson, David E. Sosnovik
Summary: The flux of autophagosomes can be measured non-invasively by using iron oxide nanoparticles decorated with fluorescent peptides and imaging techniques such as magnetic resonance imaging or near-infrared fluorescence imaging. This method enables quantitative measurements and observations of autophagy in live mouse heart tissue.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Genetics & Heredity
K. Varsha Mohan, Alaknanda Mishra, Abaranjitha Muniyasamy, Prakriti Sinha, Parul Sahu, Ashwani Kesarwani, Kshama Jain, Perumal Nagarajan, Vinod Scaria, Manisha Agarwal, Naseem S. Akhter, Chanda Gupta, Pramod Upadhyay
Summary: Our study investigates the immunological consequences in RP patients and rd1 mouse model and their impact on retinal degeneration. We found that RP patients exhibit suppressed anti-inflammatory response to self-retinal antigens, while the rd1 mouse model shows changes in ocular immune privilege mechanisms, leading to accelerated retinal degeneration.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Oncology
Kathleen M. Lennon, Andras Saftics, Sarah Abuelreich, Parul Sahu, H. Immo Lehmann, Adam L. Maddox, Reem Bagabas, James L. Januzzi, Kendall Van Keuren-Jensen, Ravi Shah, Saumya Das, Tijana Jovanovic-Talisman
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Pediatrics
Aashish Gupta, Jacob Puliyel, Bhawana Garg, Pramod Upadhyay
