Article
Agricultural Engineering
Yufei Zhang, Junhua Yun, Hossain M. Zabed, Yuan Dou, Guoyan Zhang, Mei Zhao, Mohammad J. Taherzadeh, Arthur Ragauskas, Xianghui Qi
Summary: This study improves the co-production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) by metabolic engineering of Escherichia coli and process optimization. The co-biosynthetic pathways of 3-HP and 1,3-PDO were recruited and optimized in E. coli, coupled with cofactor regeneration systems to increase product synthesis. Pathway rebalancing and block of by-product formation improved the net titer of 3-HP and 1,3-PDO. Glycerol flux towards 3-HP and 1,3-PDO synthesis was maximized by removing metabolic repression and fine-tuning the glycerol oxidation pathway. The optimized fermentation process produced 140.50 g/L of 3-HP and 1,3-PDO, with a net yield of 0.85 mol/mol.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yufei Zhang, Hossain M. Zabed, Junhua Yun, Guoyan Zhang, Yang Wang, Xianghui Qi
Summary: Sustainable bioconversion of glycerol into 3-HP and 1,3-PDO often faces challenges due to toxic intermediate accumulation. By employing modular coculture engineering and optimizing technological configurations, efficiency in production of 3-HP and 1,3-PDO can be significantly improved. The use of resting cell biocatalysis has shown enhanced glycerol utilization and product titers through pathway rebalancing and additional biotransformation steps.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Agricultural Engineering
Pseudomonas Asiatica, Trinh Thi Nguyen, Suman Lama, Satish Kumar Ainala, Mugesh Sankaranarayanan, Ashish Singh Chauhan, Jung Rae Kim, Sunghoon Park
Summary: The study demonstrates that Pseudomonas asiatica C1 can be a promising host for the production of 3-HP from glycerol by genetic editing and optimization, achieving high production efficiency and molar yield on glucose.
BIORESOURCE TECHNOLOGY
(2021)
Article
Polymer Science
Sai Nandhini Ravi, Mugesh Sankaranarayanan
Summary: This study aims to improve the co-production of propanediol (PDO) and 3-hydroxypropionic acid (3-HP) by isolating a new strain of K. quasipneumoniae MSN12 (KpMSN12) and overexpressing the aldH and dhaB genes. The results showed that the combined overexpression of aldH and dhaB in KpMSN12 led to higher 3-HP and PDO production, as well as improved sedimentation characteristics and increased susceptibility to antibiotics.
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
(2023)
Article
Biochemical Research Methods
Zihua Li, Ziyi Wu, Xuecong Cen, Yu Liu, Ye Zhang, Dehua Liu, Zhen Chen
Summary: This study introduced and engineered a cost-effective pathway for 1,3-PDO production without the need for expensive cofactors, achieving efficient accumulation in E. coli. The approach not only increased the yield significantly but also demonstrated for the first time the feasibility of producing 1,3-PDO from various carbohydrates.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hexin Liu, Peng Zhao, Pingfang Tian
Summary: In this study, the researchers engineered a CRISPRi system to convert glucose into glycerol and improve the production of 3-hydroxypropionic acid. The bi-functional strain with the engineered CRISPRi system showed an increase in glycerol and 3-HP production using glucose as the carbon source.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Thuan Phu Nguyen-Vo, Huichang Ryu, Michael Sauer, Sunghoon Park
Summary: The study demonstrates that deleting yieP gene in Klebsiella pneumoniae can enhance 3-HP tolerance and production, which is an effective strategy to improve microbial 3-HP production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Jung-Hyun Ju, Sun-Yeon Heo, Sang-Wha Choi, Young-Min Kim, Min-Soo Kim, Chul-Ho Kim, Baek-Rock Oh
Summary: Through electron beam irradiation mutagenesis, a mutant strain Lactobacillus reuteri JH83 with increased organic acid resistance was obtained, which achieved high bioconversion yield and productivity of 1,3-propanediol (1,3-PDO) in fed-batch fermentation with pure glycerol. Additionally, transcriptome analysis revealed changes in gene expression related to the defense mechanisms against organic acids during the production of 1,3-PDO.
BIORESOURCE TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Yufei Zhang, Junhua Yun, Lei Zhou, Guoyang Zhang, Mei Zhao, Hossain M. Zabed, Xianghui Qi
Summary: To achieve carbon neutrality and carbon peaking goals, sustainable biomanufacturing of high-value chemicals such as 3-hydroxypropionic acid (3-HP) through metabolic engineering of Escherichia coli has been explored. Efforts were made to optimize the biosynthetic pathway, enhance glycerol uptake, and improve the production of 3-HP through genetic engineering and process optimization. The use of corn steep liquor as a cheap fermentation medium further enhanced the production of 3-HP. Co-fermentation of crude glycerol with glucose and acetic acid resulted in high yields and productivity of 3-HP.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Applied
Sai Nandhini Ravi, Mugesh Sankaranarayanan
Summary: 3-hydroxypropionic acid (3-HP) is a valuable platform chemical with high demand. In this study, metabolic engineering of Escherichia coli was conducted to enhance 3-HP production and reduce by-product accumulation. The deletion of yqhD, poxB, and pta-ackA genes resulted in increased 3-HP titre and yield, as well as decreased synthesis of propanediol and acetate.
TOPICS IN CATALYSIS
(2023)
Article
Chemistry, Physical
Shijie Cheng, Yiqiu Fan, Xiaoxin Zhang, Yang Zeng, Songhai Xie, Yan Pei, Gaofeng Zeng, Minghua Qiao, Baoning Zong
Summary: By varying the mass ratio of the swelling agent and the template during the synthesis, a series of tungsten-doped Pt/W-MCFs catalysts were prepared, with Pt/W-1.5MCFs showing the highest 1,3-propanediol selectivity and activity. The synergy between Pt nanoparticles and single-sited tetrahedral (WO4)-O-V species at the metal-oxide interface is proposed to be responsible for the differences in catalytic performances among the Pt/W-MCFs catalysts.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2021)
Article
Biotechnology & Applied Microbiology
Albert Fina, Stephanie Heux, Joan Albiol, Pau Ferrer
Summary: This study improves the productivity of Pichia pastoris in producing 3-hydroxypropionic acid by combining different metabolic engineering approaches. The results show that, in combination with using glycerol as a carbon source, the metabolically engineered strains achieved the highest concentration and productivity of 3-hydroxypropionic acid reported so far in yeast.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Fengliang Wu, Huifang Jiang, Xuhai Zhu, Rui Lu, Lei Shi, Fang Lu
Summary: Glycerol conversion to 1,3-propanediol is an economical and sustainable approach, but traditional catalytic reactions often result in the production of 1,2-propanediol. Currently, tungsten-containing bifunctional catalysts are considered as highly effective and atom-economical catalytic systems for selectively hydrogenolysis of glycerol to 1,3-propanediol.
Review
Chemistry, Multidisciplinary
Fanghuan Zhu, Dehua Liu, Zhen Chen
Summary: This review provides a detailed overview of recent advancements in metabolic engineering and synthetic biology strategies for the biological production of 1,3-propanediol (1,3-PDO). It discusses the development of biological routes for 1,3-PDO production from glycerol, sugars, and other carbon sources using engineered producers, new chassis, and new pathways. The review also includes the exploration of microbial consortia and electro-fermentation processes. The challenges and future prospects of current bioproduction systems and their potential industrial applications are discussed.
Article
Agricultural Engineering
Junhua Yun, Hossain M. Zabed, Yufei Zhang, Amreesh Parvez, Guoyan Zhang, Xianghui Qi
Summary: The study successfully developed a co-culture method for producing 1,3-propanediol without the need for vitamin B-12, resulting in a higher yield of 1,3-PD. The co-fermentation in a 10 L bioreactor achieved a high productivity of 41.65 g/L 1,3-PD, showcasing the potential for cost-effective and efficient bioproduction.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Suman Lama, Su Moon Ro, Eunhee Seol, Balaji Sundara Sekar, Satish Kumar Ainala, Jayaraman Thangappan, Hyohak Song, Doyoung Seung, Sunghoon Park
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2015)
Article
Agricultural Engineering
Vinod Kumar, Meetu Durgapal, Mugesh Sankaranarayanan, Ashok Somasundar, Chelladurai Rathnasingh, HyoHak Song, Doyoung Seung, Sunghoon Park
BIORESOURCE TECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Zhengbin Li, Soo Moon Ro, Balaji Sundara Sekar, Eunhee Seol, Suman Lama, Sun Gu Lee, Guangyi Wang, Sunghoon Park
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2016)
Article
Biotechnology & Applied Microbiology
Mugesh Sankaranarayanan, Ashok Somasundar, Eunhee Seol, Ashish Singh Chauhan, Seongjin Kwon, Gyoo Yeol Jung, Sunghoon Park
JOURNAL OF BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Mugesh Sankaranarayanan, Eunhee Seol, Yeonhee Kim, Ashish Singh Chauhan, Sunghoon Park
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Jae Hyeon Lee, Suman Lama, Jung Rae Kim, Sung Hoon Park
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2018)
Article
Biotechnology & Applied Microbiology
Thuan Phu Nguyen-Vo, Yunxiao Liang, Mugesh Sankaranarayanan, Eunhee Seol, Ah Yeong Chun, Somasundar Ashok, Ashish Singh Chauhan, Jung Rae Kim, Sunghoon Park
METABOLIC ENGINEERING
(2019)
Article
Agricultural Engineering
Shengfang Zhou, Suman Lama, Jihong Jiang, Mugesh Sankaranarayanan, Sunghoon Park
BIORESOURCE TECHNOLOGY
(2020)
Article
Agricultural Engineering
Pseudomonas Asiatica, Trinh Thi Nguyen, Suman Lama, Satish Kumar Ainala, Mugesh Sankaranarayanan, Ashish Singh Chauhan, Jung Rae Kim, Sunghoon Park
Summary: The study demonstrates that Pseudomonas asiatica C1 can be a promising host for the production of 3-HP from glycerol by genetic editing and optimization, achieving high production efficiency and molar yield on glucose.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yeonhee Kim, Suman Lama, Deepti Agrawal, Vinod Kumar, Sunghoon Park
Summary: Acetate is recognized as a promising carbon feedstock in industrial biotechnology, and this review provides a detailed analysis of acetate metabolism in biochemical, microbial, and biotechnological aspects, as well as summarizing the latest technologies in producing value-added chemicals from acetate.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biotechnology & Applied Microbiology
Suman Lama, Eunhee Seol, Sunghoon Park
METABOLIC ENGINEERING
(2020)
Article
Agricultural Engineering
Suman Lama, Eunhee Seol, Sunghoon Park
BIORESOURCE TECHNOLOGY
(2017)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)