Review
Agricultural Engineering
Jitendra Kumar, Himanshu, Hemansi, Amanjot Kaur, Aayush Mathur
Summary: Global interest in lignocellulosic biorefineries has increased due to sustainable technological advancements. However, enzymatic hydrolysis in biorefinery operations faces challenges that limit cost-effectiveness.
BIORESOURCE TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Yufeng Yuan, Bo Jiang, Hui Chen, Wenjuan Wu, Shufang Wu, Yongcan Jin, Huining Xiao
Summary: Enzymatic hydrolysis of lignocellulose for bioethanol production has great potential, but the presence of lignin inhibits the process. Pretreatment methods can remove lignin and improve enzymatic digestibility. Researchers are focusing on exploring the relationship between lignin structure and lignin-enzyme interactions to enhance saccharification efficiency.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Green & Sustainable Science & Technology
Cheng Cai, Chaofeng Zhang, Ning Li, Huifang Liu, Jun Xie, Hongming Lou, Xuejun Pan, J. Y. Zhu, Feng Wang
Summary: Hydrolyzing lignocellulose and transforming it into chemicals and fuels is an essential aspect of biorefining. Previously, lignin was believed to inhibit the enzymatic hydrolysis of lignocellulosic substrates. However, recent research has shown that under specific conditions, lignin can actually increase the sugar yield and act as an activator/promoter of enzymatic hydrolysis. Furthermore, lignin-based carriers have been developed to reduce the load and cost of cellulases.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Feiyun Li, Huinan Liang, Jinxian Shan, Aiting Zhang, Hongming Lou, Yanjun Tang
Summary: The cost of lignocellulosic enzymatic hydrolysis was reduced by enhancing enzymatic hydrolysis and recycling cellulase using lignin-grafted quaternary ammonium phosphate (LQAP) with temperature and pH response. LQAP dissolved under the hydrolysis condition and enhanced the hydrolysis. After hydrolysis, LQAP and cellulase co-precipitated and could be recovered by lowering pH and cooling. LQAP showed significant pH-UCST response, enzymatic hydrolysis enhancement, and cellulase recovery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agricultural Engineering
Qiang Xiong, Jie Qiao, Minghui Wang, Shuang Li, Xiujuan Li
Summary: This study investigated the effects of carboxylated and quaternized lignin (CQL) and hydroxymethylated lignin (HML) on enzymatic hydrolysis efficiency (EHE) of p-toluenesulfonic acid treated corn stover (PCS). The results showed that CQL enhanced EHE by forming CQL-I3-GL complexes with lower binding free energy, while HML decreased EHE due to overlapping binding sites with the carbohydrate binding domain of I3-GL, resulting in decreased enzyme activity. This research helps in understanding the impact of modified lignins on EHE and cellulase adsorption.
BIORESOURCE TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Lan Wang, Yongxin Bu, Lele Sun, Hongzhang Chen
Summary: Combining Fenton reagent with cellulase can improve lignocellulose degradation efficiency and reduce enzyme dosage, thus reducing the production cost of lignocellulosic ethanol.
Article
Agricultural Engineering
Mohan Li, Yufeng Yuan, Yangsu Zhu, Bo Jiang, Wenjuan Wu, Shufang Wu, Yongcan Jin
Summary: The addition of sulfomethylated lignins in enzymatic hydrolysis significantly improved substrate digestibility and sugar yield. Competitive adsorption between cellulase, substrate lignin, and SLs was suggested as a mechanism for the observed effects.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Xindong Chen, Hailong Li, Shimiao Yao, Can Wang, Xuefang Chen, Haijun Guo, Lian Xiong, Hairong Zhang, Xinde Chen
Summary: This study investigated the impact of cellulose ultrastructure on the inhibition of lignin during enzymatic hydrolysis. It also explored the effects of changing cellulose ultrastructure on cellulose conversion. The results showed that cellulose ultrastructure significantly influenced the degree of lignin inhibition.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Feiyun Li, Haohao Li, Hongming Lou, Fubao Sun, Yanjun Tang
Summary: The enzymatic hydrolysis cost of lignocellulose can be reduced by improving enzymatic hydrolysis and recycling cellulase by adding additives. P(SSS-co-SPE) copolymers were synthesized and exhibited upper critical solution temperature response. PSSP with high molar ratio of SSS displayed improved hydrolysis performance. When added to the hydrolysis system of corncob residues, PSSP increased the substrate enzymatic digestibility and saved cellulase amount, providing a new idea for reducing the hydrolysis cost of lignocellulose-based sugar platform technology.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Hongliang Guo, Ying Zhao, Jo-Shu Chang, Duu-Jong Lee
Summary: Enzymatic hydrolysis is crucial for efficient biorefinery of lignocellulosic biomass. Understanding the properties of key enzymes and their interactions with biomass is essential for improving the process efficiency. This article provides an update on the research of key enzymes in lignocellulose biorefinery, including their properties, interactions with biomass, adsorption, and hydrolysis. The advanced analytical techniques for investigating these interactions and future research prospects are discussed.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Min Zhou, Meiqi Lv, Sulin Cai, Xingjun Tian
Summary: DESs were synthesized and evaluated for their physicochemical properties. Herbaceous and woody lignocellulose biomass were selected as substrates for pretreatment. Microstructural changes of pretreated lignocellulose were analyzed using SEM, FT-IR, and XRD. The efficient delignification increased enzyme accessibility to cellulose, while lignin content negatively affected enzymatic hydrolysis conversion. GPC analysis showed a reduction in sulfonated lignin polydispersity after pretreatment.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Hexue Jia, Wan Sun, Xuezhi Li, Jian Zhao
Summary: The study cloned, expressed and purified Cip1 from T. reesei, and investigated its effects on enzymatic hydrolysis of different pretreated lignocellulose. It showed that Cip1 could significantly promote the enzymatic hydrolysis, especially on substrates with high lignin content. Additionally, Cip1 could disrupt the crystal structure of cellulose and reduce non-productive adsorption of cellulase on lignin.
MICROBIAL CELL FACTORIES
(2021)
Review
Biotechnology & Applied Microbiology
Rui Zhai, Jinguang Hu, Mingjie Jin
Summary: Lignocellulosic biorefinery based on sugar-platform is considered an efficient strategy to replace fossil fuel-based refinery. However, various inhibitors, such as phenolic compounds, are generated during the pretreatment process, severely affecting the subsequent enzymatic hydrolysis and fermentation. This review summarizes the formation mechanism of phenolics in different pretreatment methods and discusses the key factors influencing their inhibitory effects. It also introduces recent achievements in inhibition mitigation strategies to enhance cellulose hydrolysis for sugar-platform biorefinery.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Agricultural Engineering
Xinyuan Zhang, Yiqin Wang, Jie Lu, Miaomiao Liu, Wanting Tan, Yi Cheng, Yehan Tao, Jian Du, Haisong Wang
Summary: This paper used five different types of biosurfactants to enhance the saccharification efficiency in the enzymatic hydrolysis process. The addition of sophorolipid increased the enzymatic hydrolysis efficiency by 30.65%, while the combination of sophorolipid and tea saponin further increased the efficiency to 99.56%. These biosurfactants acted synergistically to inhibit enzymatic inactivation and non-productive adsorption of lignin. This study proposes an effective method to improve enzymatic hydrolysis efficiency and reduce enzyme dosage by utilizing biosurfactants.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Mingyang Hu, Lin Yuan, Ziyuan Cai, Weihua Zhang, Qiang Fu, Dandan Ji
Summary: In this study, a new pretreatment method combining ammonia fiber expansion (AFEX) and deep eutectic solvent (DES) was developed for the rapid separation of wheat straw fractions. The AFEX-assisted DES pretreatment significantly reduced pretreatment time and improved pretreatment efficiency. The study also investigated the enzymatic effect of cellulose on glucose generation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)