期刊
BIOCHIMIE
卷 164, 期 -, 页码 60-69出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2019.07.003
关键词
Post-transcriptional tRNA modifications; Staphylococcus aureus; 2D gel isolation; nanoLC/MSMS
资金
- Agence Nationale de la Recherche (ANR) [ANR-16-CE11-0007-01, ANR-15-CE11-0021-01]
- Fonds Regional de Cooperation Pour la Recherche (Region Grand Est, project EpiRNA)
- French National Research Agency [LABEX: ANR-10-LABX-0036 NETRNA, ANR-17-EURE-0023]
- labEx NetRNA
- Grand Est region
- Agence Nationale de la Recherche (ANR) [ANR-16-CE11-0007, ANR-17-EURE-0023] Funding Source: Agence Nationale de la Recherche (ANR)
RNA modifications are involved in numerous biological processes. These modifications are constitutive or modulated in response to adaptive processes and can impact RNA base-pairing formation, protein recognition, RNA structure and stability. tRNAs are the most abundantly modified RNA molecules. Analysis of the roles of their modifications in response to stress, environmental changes, and infections caused by pathogens, has fueled new research areas. Nevertheless, the detection of modified nucleotides in RNAs is still a challenging task. We present here a reliable method to identify and localize tRNA modifications, which was applied to the human pathogenic bacteria, Staphyloccocus aureus. The method is based on a separation of tRNA species on a two-dimensional polyacrylamide gel electrophoresis followed by nano liquid chromatography-mass spectrometry. We provided a list of modifications mapped on 25 out of the 40 tRNA species (one isoacceptor for each amino acid). This method can be easily used to monitor the dynamics of tRNA modifications in S. aureus in response to stress adaptation and during infection of the host, a relatively unexplored field. (C) 2019 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
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