Article
Biochemistry & Molecular Biology
Anne M. Skelton, D. Joshua Cohen, Barbara D. Boyan, Zvi Schwartz
Summary: This study aimed to investigate the characteristics of osteoblast-derived matrix vesicles (MV) in the regulation of extracellular vesicles and osteoblast differentiation, and to assess their mechanism of uptake. The results showed that MVs exhibited increased alkaline phosphatase activity, specific size and membrane protein markers, and played a regulatory role in osteoblast differentiation and production of inflammatory markers. The uptake of MVs by osteoblasts was found to involve a vesicular transport mechanism. These findings support the hypothesis that osteoblast-derived MVs participate in the regulation of osteogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Medicinal
Sana Ansari, Bregje W. M. de Wildt, Michelle A. M. Vis, Carolina E. de Korte, Keita Ito, Sandra Hofmann, Yuana Yuana
Summary: MtVs, including phosphatases, calcium, and inorganic phosphate, play a crucial role in matrix mineralization and possess bone-targeting and osteo-inductive properties.
Article
Cell & Tissue Engineering
Genzheng Yi, Siyuan Zhang, Yue Ma, Xueting Yang, Fangjun Huo, Yan Chen, Bo Yang, Weidong Tian
Summary: This study found that matrix vesicles (MVs) can effectively improve the proliferation, migration, and osteogenic differentiation of dental follicle cells (DFCs), with collagenase-released MVs (CRMVs) showing better bone regeneration capabilities. In a rat alveolar bone defect model, CRMVs loaded on a collagen scaffold had more significant therapeutic outcomes compared to media MVs (MMVs) and DFCs-based treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Zheng Li, Muxin Yue, Xuenan Liu, Yunsong Liu, Longwei Lv, Ping Zhang, Yongsheng Zhou
Summary: This study reveals the role of extracellular matrix stiffness in regulating stem cell fate and the novel mechanism of mitochondrial phosphoenolpyruvate carboxykinase in promoting osteogenesis in 3D matrix. These findings provide new perspectives for strategies of osteoporosis.
BIOACTIVE MATERIALS
(2022)
Review
Cell Biology
Jie Yang, Tong Pei, Guanyue Su, Peiyan Duan, Xiaoheng Liu
Summary: The mineralization of the extracellular matrix (ECM) is crucial for bone formation and mineralization-related diseases. AnnexinA6 (AnxA6) has been identified as a key protein involved in this process and may serve as a potential target for ECM mineralization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Weifei Zhang, Pengzhou Huang, Jianjing Lin, Hui Zeng
Summary: Osteoporosis is a common metabolic bone disease characterized by high incidence rates. Extracellular vesicles (EVs) play a significant role in the pathogenesis of osteoporosis, and understanding their role can provide valuable insights for the prevention and treatment of osteoporosis.
Article
Medicine, Research & Experimental
Ba Huang, Yongwei Su, Enpu Shen, Meng Song, Danping Liu, Hui Qi
Summary: The study demonstrated that GPNMB-EVs significantly promoted the proliferation and osteogenic differentiation of BMSCs, attenuated osteoporosis by activating the Wnt/beta-catenin signaling pathway. In vivo experiments showed remarkable improvement in trabecular bone regeneration in the OVX-induced rat model of OP.
Article
Engineering, Biomedical
Liang Ma, Wencan Ke, Zhiwei Liao, Xiaobo Feng, Jie Lei, Kun Wang, Bingjin Wang, Gaocai Li, Rongjin Luo, Yunsong Shi, Weifeng Zhang, Yu Song, Weibin Sheng, Cao Yang
Summary: Nanotopographical cues can guide bone regeneration through the memory function of small extracellular vesicles (sEV). In this study, sEVs were extracted from human mesenchymal stem cells (hMSC) cultured on nanotopographical titanium plates, and it was found that they had superior pro-osteogenesis ability. RNA sequencing further confirmed the mechanisms of sEVs in bone regeneration. Finally, sEVs were applied to a scaffold to promote bone ingrowth.
BIOACTIVE MATERIALS
(2022)
Article
Cell Biology
Jifeng Xu, Yu Chen, Dongsheng Yu, Li Zhang, Xiaofan Dou, Gang Wu, Yaping Wang, Shuijun Zhang
Summary: The study revealed that osteoporotic patients had significantly higher concentrations of EVs in plasma, which showed a linear correlation with bone mineral density values. The expression levels of miRNAs and Wnt signals were associated with osteoporosis, indicating that osteoporosis increases the secretion of EVs that carry specific molecules affecting mineralization capacity.
Article
Cell Biology
Meredith Whitehead, Syabira Yusoff, Sadia Ahmad, Lukas Schmidt, Manuel Mayr, Jillian Madine, David Middleton, Catherine M. Shanahan
Summary: Vascular amyloidosis, caused by the aggregation of peptide monomers into insoluble amyloid, is a common age-related pathology. This study found that extracellular vesicles (EVs) and HSPG2 in the extracellular matrix (ECM) play key roles in medin accumulation, contributing to the development of age-associated aortic medial amyloid (AMA).
Article
Biochemistry & Molecular Biology
Martina Gatti, Francesca Beretti, Manuela Zavatti, Emma Bertucci, Soraia Ribeiro Luz, Carla Palumbo, Tullia Maraldi
Summary: The study found that EV secreted by human amniotic fluid stem cells can improve the differentiation ability of pre-osteoblasts, increase cell viability, and reduce apoptotic markers. Moreover, EV positively modulates the oxidative stress induced by dexamethasone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Correction
Materials Science, Biomaterials
Shiqing Ma, Xuewen Li, Han Hu, Xinying Ma, Zhezhe Zhao, Shu Deng, Jie Wang, Leyu Zhang, Chenxuan Wu, Zihao Liu, Yonglan Wang
Summary: In this study, the authors demonstrated the synergistic osteogenesis repair achieved by combining RGD-colonized intestinal bacteria on the surface of 3D-printed titanium implants and extracellular vesicles.
BIOMATERIALS SCIENCE
(2023)
Article
Engineering, Biomedical
Raphael J. Crum, Hector Capella-Monsonis, Jordan Chang, Marley J. Dewey, Brian D. Kolich, Kelsey T. Hall, Salma O. El-Mossier, David G. Nascari, George S. Hussey, Stephen F. Badylak
Summary: Matrix-bound nanovesicles (MBV) are a distinct subtype of bioactive extracellular vesicles that are embedded within the extracellular matrix (ECM). Recent studies have demonstrated the therapeutic potential of MBV in models of retinal damage and rheumatoid arthritis by targeting immunomodulation. However, the biocompatibility and biodistribution of MBV in vitro and in vivo have not been fully characterized. This study comprehensively examined the pre-clinical safety profile of MBV and found that they are well-tolerated, non-toxic, and non-immunosuppressive. These findings highlight the translational feasibility of MBV therapeutics for various clinical applications.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Kenny Man, Mathieu Y. Brunet, Sophie Louth, Thomas E. Robinson, Maria Fernandez-Rhodes, Soraya Williams, Angelica S. Federici, Owen G. Davies, David A. Hoey, Sophie C. Cox
Summary: The study showed that 3D printed titanium scaffolds coated with hydroxyapatite can enhance the therapeutic efficacy of osteoblast-derived EVs. Triangle pore scaffolds significantly increased osteoblast mineralization and EV yield, demonstrating the potential for bone repair. Coating with bone-mimetic nano-needle hydroxyapatite further improved osteoblast mineralization and EV production, highlighting the importance of harnessing bone-mimetic culture platforms for regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Zhengyu Yang, Xiaodong Liu, Fujian Zhao, Mengyu Yao, Zefeng Lin, Zhen Yang, Cong Liu, Yuwei Liu, Xiaofeng Chen, Chang Du
Summary: This study reveals a new mechanism by which bioactive glass nanoparticles (BGN) regulate bone loss through inducing the secretion of extracellular vesicles (EVs) by bone marrow mesenchymal stem cells (BMSCs). The EVs inhibit osteoclast differentiation by reducing the nuclear translocation of NFATc1. Furthermore, BGN+BMSC-EVs show potential in alleviating bone loss in an osteoporosis mouse model, with no acute systemic toxicity.
Article
Nanoscience & Nanotechnology
Haijing Shen, Yingqian Wang, Jie Wang, Zhihao Li, Quan Yuan
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Chemistry, Analytical
Jie Wang, Yingqian Wang, Xiaoxia Hu, Chunli Zhu, Qinqin Ma, Ling Liang, Zhihao Li, Quan Yuan
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Jie Tan, Mengmeng Zhao, Jie Wang, Zhihao Li, Ling Liang, Liqin Zhang, Quan Yuan, Weihong Tan
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Multidisciplinary
Meng Liu, Zhihao Li, Yingxue Li, Jiajia Chen, Quan Yuan
CHINESE CHEMICAL LETTERS
(2019)
Article
Dentistry, Oral Surgery & Medicine
Richard J. Miron, Hudi Xu, Jihua Chai, Jiaolong Wang, Shihang Zheng, Mengge Feng, Xiaoxin Zhang, Yan Wei, Yan Chen, Carlos Fernando de Almeida Barros Mourao, Anton Sculean, Yufeng Zhang
CLINICAL ORAL INVESTIGATIONS
(2020)
Article
Nanoscience & Nanotechnology
Lingling Zhang, Yulan Wang, Can Wang, Ming He, Jiangshan Wan, Yan Wei, Jinglun Zhang, Xiangliang Yang, Yanbing Zhao, Yufeng Zhang
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Multidisciplinary
Cuizhu Tang, Yan Wei, Lin Gu, Qinghua Zhang, Mei Li, Guohua Yuan, Yuan He, Li Huang, Yan Liu, Yufeng Zhang
Article
Chemistry, Multidisciplinary
Yan Wei, Miusi Shi, Jinglun Zhang, Xiaoxin Zhang, Kailun Shen, Rui Wang, Richard J. Miron, Yin Xiao, Yufeng Zhang
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Jiajia Chen, Huajian Gao, Zhihao Li, Yingxue Li, Quan Yuan
CHINESE CHEMICAL LETTERS
(2020)
Article
Chemistry, Multidisciplinary
Qin Zhao, Zijian Gong, Zhihao Li, Jinyang Wang, Jinglun Zhang, Zifan Zhao, Peng Zhang, Shihang Zheng, Richard J. Miron, Quan Yuan, Yufeng Zhang
Summary: The study introduces a lysosome-targeting nanoparticle developed from a mineralized metal-organic framework coupled with a lysosome-targeting aptamer to enhance the antitumor effect of T cells. The MOF synthesized from Zn2+ and dimethylimidazole has good protein encapsulation and acid sensitivity, making it an ideal lysosomal delivery vector. The use of calcium carbonate to induce MOF mineralization improves stability and provides synergistic effects for encapsulating therapeutic proteins.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Haoran Liu, Zhihao Li, Ruichen Shen, Zhiheng Li, Yanbing Yang, Quan Yuan
Summary: Urinary tract infections caused by bacterial invasion can be life-threatening and pose a significant health threat to over 150 million people globally. An intelligent diagnostic system using an immunobiosensor, signal-amplification biochip, and machine vision-based image processing algorithm has been developed for accurate and rapid bacterial detection, offering a potentially affordable, user-friendly digital diagnostic solution with enhanced luminescence and wide dynamic range.
Article
Chemistry, Multidisciplinary
Zhihao Li, Huajian Gao, Ruichen Shen, Caixin Zhang, Leisi Li, Yawei Lv, Liming Tang, Yaping Du, Quan Yuan
Summary: The study presents a macromolecule-mediated strategy for the assembly of facet-selective UCNPs/2DMOFs nanostructures, which show specific optical properties and advantages in anti-counterfeiting. The demonstrated nanostructures offer a vivid example for controlled fabrication of facet-selective nanostructures and can promote the development of advanced functional materials for applications in biosensing, energy conversion, and information assurance.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Zhihao Li, Haoran Liu, Rui Wang, Chenhui Ji, Yan Wei, Miusi Shi, Yingqian Wang, Yaping Du, Yufeng Zhang, Quan Yuan, Chunhua Yan
Review
Chemistry, Multidisciplinary
Qiaosong Lin, Zhihao Li, Chenhui Ji, Quan Yuan
NANOSCALE ADVANCES
(2020)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
