Article
Chemistry, Analytical
Thi Ngoc Diep Trinh, Duc Anh Thai, Nae Yoon Lee
Summary: The study developed a paper-based microdevice for VRE identification, integrating DNA extraction, LAMP amplification, and colorimetric detection. The device achieved highly sensitive detection with a limit of VRE detection of 102 CFU/mL in milk and holds promise for point-of-care applications in medical and food safety settings. The microdevice allows folding on a 3D paper platform for sample mixing and sequential DNA purification and amplification, providing accurate and rapid diagnosis and control of VRE.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Duc Anh Thai, Seung Kyun Park, Nae Yoon Lee
Summary: This study introduces a thermoplastic microdevice integrated with additive-enhanced allele-specific amplification and hydrazine-induced silver nanoparticle-based detection of single nucleotide polymorphism (SNP) and opportunistic pathogens. The microdevice allows for purification, amplification, and detection of DNA in a sequential manner. The results demonstrate the successful identification of SNPs related to sickle cell anemia, hair loss, and Enterococcus faecium. The microdevice has a versatile applicability and shows promise for point-of-care nucleic acid testing in low-resource settings.
Article
Chemistry, Multidisciplinary
Poulomi Biswas, Giri Nandagopal Mukunthan Sulochana, Theneyur Narayanaswamy Banuprasad, Pankaj Goyal, Dolonchampa Modak, Ananta Kumar Ghosh, Suman Chakraborty
Summary: A multiplex paper/polymer-based detection strip has been developed for early detection of all four serotypes of dengue viruses, which can facilitate disease management and patient recovery.
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Review
Biotechnology & Applied Microbiology
Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li, Chenze Lu
Summary: As nucleic acid testing becomes crucial in various research fields, there is an increasing demand for rapid on-site testing methods. This review discusses recent advances in on-chip methods for sample preparation, amplification, and detection in the nucleic acid testing procedure. It covers integration strategies for sample preparation, various on-chip amplification methods, and evaluation of potential detection methods. The review also highlights the importance of improving the speed and integration of all steps onto a single chip for future developments in nucleic acid testing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yiren Cao, Jinjun Wu, Bo Pang, Hongquan Zhang, X. Chris Le
Summary: The trans-cleavage activity of target-activated CRISPR/Cas12a released an RNA crosslinker from a molecular transducer, aiding in the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a allowed for visual detection of the N gene and E gene of SARS-CoV-2 in 45 minutes.
CHEMICAL COMMUNICATIONS
(2021)
Article
Materials Science, Multidisciplinary
Georgios Chondrogiannis, Pedro Reu, Mahiar M. Hamedi
Summary: Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics, but it is mainly limited to centralized laboratories. Researchers have addressed the bottleneck issue of DNA extraction by immobilizing the enzyme ACP on nitrocellulose, enabling home-based kits and expanding the capabilities of home-testing beyond antigen tests.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Multidisciplinary Sciences
Lukas Bokelmann, Olaf Nickel, Tomislav Maricic, Svante Paabo, Matthias Meyer, Stephan Borte, Stephan Riesenberg
Summary: Cap-iLAMP combines RNA extraction with improved RT-LAMP color scoring to detect SARS-CoV-2 positive samples in less than an hour, reducing reagent costs per test.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa, Manish Biyani
Summary: This new isothermal nucleic acid amplification assay, called RICCA, allows for rapid and direct detection of viruses with low viral loads. It has shown high sensitivity in detecting RNA viruses directly from saliva samples and synthetic templates of SARS-CoV-2. The assay can be used in real-time point-of-care testing and has a total assay time of 15-30 minutes for detecting heat-inactivated SARS-CoV-2 virus in human saliva.
SCIENTIFIC REPORTS
(2021)
Article
Nanoscience & Nanotechnology
Xiaoqian Liu, Jing Sun, Yanhong Tong, Mingxin Zhang, Xue Wang, Shanlei Guo, Xu Han, Xiaoli Zhao, Qingxin Tang, Yichun Liu
Summary: This research demonstrates the development of an eco-friendly all-paper touch-temperature sensor using renewable raw materials, which can be freely cut or folded into different shapes, morph between 2D and 3D configurations, and exhibit discrimination capability towards pressure and temperature. The use of cost-effective common commodities in daily life reduces costs, has positive impacts on the environment and health, while maintaining the structural integrity and device functionality.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Yeqing Wan, Gaiping Li, Lina Zou, Hong Wang, Qing Wang, Kaiyue Tan, Xiaoqing Liu, Fuan Wang
Summary: A self-catalytic DNA assembly (SDA) machine was developed for programmable assembly of functional DNA nanostructures in living cells, enabling sensitive detection of cancer biomarkers such as miR-21. The system combines catalytic self-assembly (CSA) and DNAzyme amplification modules to achieve a self-catalytic amplification reaction, leading to precise intracellular imaging and potential for early cancer diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Kamal G. Shah, Sujatha Kumar, Paul Yager
Summary: This study demonstrates how near-digital nucleic acid amplification tests can be implemented in low-cost porous media to approach the low limits of detection of digital assays, capturing many benefits of digital NAATs when performed in a paper membrane.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Jan Gorm Lisby, Uffe Vest Schneider
Summary: Traditional organism-growth based diagnosis of acute infections is a major challenge, but new molecular diagnostic technologies can provide rapid results in a near-patient setting. This technology has the potential for positive impacts on clinical patient management and antibiotic stewardship, but also comes with risks.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biophysics
Jingjing Qian, Qinming Zhang, Meng Lu
Summary: In recent years, paper-based nucleic acid sensors have been used to detect DNA and RNA molecules extracted from viruses and bacteria. These sensors require the release of nucleic acids from lipid membranes and the removal of interfering molecules for accurate analysis. A portable sensor system has been developed to combine thermolysis and paper-based nucleic acid amplification tests (NAATs) for the detection of viral and exosomal RNA molecules. The system achieved quantitative analysis of influenza A virus (IAV) RNA and exosome microRNA within 1 hour, making it a promising point-of-care test for viral and bacterial infections.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Analytical
Thi Ngoc Diep Trinh, Nae Yoon Lee
Summary: This review discusses the current status and challenges in antimicrobial susceptibility testing, as well as the application advantages of microfluidic technology in this field. It also outlines the methods of genotypic antimicrobial susceptibility testing and the advancements in microdevices.
Article
Chemistry, Analytical
Kieu The Loan Trinh, Nae Yoon Lee
Summary: In this study, a wearable nucleic acid amplification microdevice was developed, which utilizes human body heat for reaction and provides an instrument-free solution for DNA amplification in resource-limited settings. The device is flexible, adhesive, and capable of rapid amplification.
Article
Chemistry, Multidisciplinary
Rajamanickam Sivakumar, Vu Phong Dinh, Nae Yoon Lee
Summary: We developed a colorimetric strategy based on copper sulfate (CuSO4)-initiated diphenylamine (DPA) coupled with loop-mediated isothermal amplification (LAMP) for rapid detection of SARS-CoV-2 and Enterococcus faecium. Acid hydrolysis of LAMP amplicons was used to detect the DNA and generate a blue color. The method successfully detected low quantities of SARS-CoV-2 RNA and E. faecium, indicating its advantages. Multiplex detection of pathogens was performed using a foldable microdevice, confirming the practical applicability of the method.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Chemistry, Analytical
Hoang Lan Pham, Da Hyun Yang, Woo Ri Chae, Jong Hyeok Jung, Thi Xoan Hoang, Nae Yoon Lee, Jae Young Kim
Summary: In this study, a new method for generating regulatory macrophage (Mreg)-like cells from THP-1 cells was established using a three-dimensional micropatterned polydimethylsiloxane (PDMS) surface coated with polydopamine (PDA) and arginylglycylaspartic acid (RGD). The coated 3D micropatterns induced the most elongated morphology and strongest expression of Mreg markers in the cells within four days without the use of cytokines, providing a time- and cost-effective approach for Mreg generation in vitro.
Review
Chemistry, Multidisciplinary
Nguyen Nhat Nam, Hoang Dang Khoa Do, Kieu The Loan Trinh, Nae Yoon Lee
Summary: The first report of deep eutectic solvents (DESs) in 2003 introduced them as a new type of ionic liquid (IL) with innovative chemical and physical characteristics. DESs, using green solvent technology, provide a sustainable approach for nanomaterial fabrication, allowing for the design of nanostructures with specific morphology and properties by adjusting reaction conditions. Their use in synthesis reactions reduces energy consumption and environmental contamination risks. This review paper discusses and highlights the recent applications and advancements in the use of DESs for green synthesis of nanomaterials, including wet-chemical processes, nanosize reticular material fabrication, electrodeposition/electrochemical synthesis of nanostructures, electroless deposition, DESs-based nano-catalytic systems, and nanofluidic systems.
Article
Chemistry, Multidisciplinary
Rajamanickam Sivakumar, So Yeon Park, Nae Yoon Lee
Summary: Here, a quercetin-mediated silver nanoparticle (AgNP) synthesis combined with loop-mediated isothermal amplification (LAMP) technique was used to detect SARS-CoV-2 and Enterococcus faecium using a foldable PMMA microdevice. The LAMP amplicons formed a complex with Ag+ ions, while the quercetin acted as a reducing agent and chelated with Ag+ ions, resulting in the formation of brown-colored AgNPs within 5 minutes. The method exhibited higher sensitivity than agarose gel electrophoresis and had a detection limit of 101 copies μL-1 for SARS-CoV-2 RNA and 101 CFU mL-1 for E. faecium.
Review
Food Science & Technology
Nguyen Nhat Nam, Hoang Dang Khoa Do, Kieu The Loan Trinh, Nae Yoon Lee
Summary: In the era of omics, various fields have been identified, including genomics, proteomics, transcriptomics, metabolomics, phenomics, and metagenomics. Metagenomics, among them, has significantly contributed to discoveries related to the microbial world. Newly discovered microbiomes in different ecologies provide valuable information on microbial diversity and functions on Earth. Therefore, metagenomics has led to new microbe-based applications in human health, agriculture, and the food industry. This review summarizes recent advances in bioinformatic tools and explores the current applications of metagenomics in various fields. Furthermore, it discusses the future perspectives of metagenomics as a powerful tool for studying the microbial world.
Review
Chemistry, Analytical
Kieu The Loan Trinh, Hoang Dang Khoa Do, Nae Yoon Lee
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered a demand for accurate and cost-effective diagnostic tests for COVID-19 and other viral infections. Traditional virus screening methods have limitations in terms of equipment, personnel, and analysis time. Microfluidic-based biosensors, integrating microfluidic and biosensor technologies, offer a platform for sample preparation and simultaneous detection with high sensitivity, accuracy, portability, and real-time results. This review presents recent advances in microfluidic-based biosensors for virus detection, categorizing them into immunoassays and molecular assays, and analyzes available commercial sensing tests.
Article
Chemistry, Analytical
So Yeon Park, Dong-Sik Chae, Jae Sun Lee, Byung-Ki Cho, Nae Yoon Lee
Summary: This study proposes a simple point-of-care testing (POCT) kit for the detection of the MTF1 OA biomarker to achieve on-site clinical diagnosis of osteoarthritis (OA). The kit contains an FTA card for patient sample treatments, a sample tube for loop-mediated isothermal amplification (LAMP), and a phenolphthalein-soaked swab for naked eye detection. The introduced method is expected to serve as a POCT platform directly applicable by clinicians for easy and rapid identification of OA.
Article
Materials Science, Multidisciplinary
Kieu The Loan Trinh, Duc Anh Thai, Da Hyun Yang, Nae Yoon Lee
Summary: Researchers developed a biocompatible adhesive using a chitosan-vitamin C hydrogel for bonding poly-(methylmethacrylate) substrates. Hydrogen bonding and ionic cross-linking between the hydrogel and PMMA were identified as the main chemical interactions enabling robust bonding. The modified PMMA surfaces exhibited enhanced hydrophilicity, resulting in a maximum bond strength of 1.48 MPa and leak-proof microchannels. The adhesive was successfully applied in the on-chip culturing of mesenchymal stem cells, promoting the formation of MSC spheroids.
ACS APPLIED POLYMER MATERIALS
(2023)
Review
Chemistry, Medicinal
Thi Ngoc Diep Trinh, Hoang Dang Khoa Do, Nguyen Nhat Nam, Thach Thi Dan, Kieu The Loan Trinh, Nae Yoon Lee
Summary: Droplet-based microfluidics has wide applications in various fields, offering advantages such as fast reaction, high sensitivity, high throughput, and low risk of cross-contamination. This review focuses on the impact of droplet microfluidics, particularly in drug screening and development. It discusses device fabrication methods, droplet generation/manipulation techniques, and highlights promising studies on drug synthesis and delivery in the last 5 years.
Correction
Materials Science, Multidisciplinary
Kieu The Loan Trinh, Duc Anh Thai, Da Hyun Yang, Nae Yoon Lee
ACS APPLIED POLYMER MATERIALS
(2023)
Review
Chemistry, Analytical
Rajamanickam Sivakumar, Nae Yoon Lee
Summary: Conventional molecular assay-based point-of-care (POC) diagnostic test consists of DNA extraction, amplification, and amplicon detection. DNA extraction is costly and time-consuming but crucial for disease identification. This review summarizes the advantages and disadvantages of DNA extraction methods and discusses the application of POC pathogen testing. It also highlights the challenges and future perspectives for integrated POC devices.
Article
Chemistry, Analytical
Hanh An Nguyen, Nae Yoon Lee
Summary: A paper device that integrates DNA extraction, loop-mediated isothermal amplification (LAMP), and detection is developed for accurate recognition of infectious pathogens without using pipettes. The device operates simply via sliding and folding, and the results can be observed by the naked eye. The optimized concentrations of key reagents were determined, and the device successfully detected Enterococcus faecium at low concentrations within 60 minutes.
Article
Biochemical Research Methods
Duc Anh Thai, Seung Kyun Park, Nae Yoon Lee
Summary: This study introduces a thermoplastic microdevice integrated with additive-enhanced allele-specific amplification and hydrazine-induced silver nanoparticle-based detection of single nucleotide polymorphism (SNP) and opportunistic pathogens. The microdevice allows for purification, amplification, and detection of DNA in a sequential manner. The results demonstrate the successful identification of SNPs related to sickle cell anemia, hair loss, and Enterococcus faecium. The microdevice has a versatile applicability and shows promise for point-of-care nucleic acid testing in low-resource settings.
Article
Chemistry, Analytical
Selva Sharma Arumugam, Amal Wilson Varghese, Swathy Suresh Nair, Nae Yoon Lee
Summary: In this study, cationic carbon dots were prepared and used to trigger the aggregation and dispersion of gold nanoparticles for colorimetric detection of nucleic acids. The method relies on the interaction between DNA and carbon dots, leading to a noticeable color change from purple to red. The colorimetric assay showed a linear response within a DNA concentration range of 0.7-14 nM.
ANALYTICAL METHODS
(2023)