High-Throughput and Direct Sample Screening Using a Laser Spray Ionization Miniature Mass Spectrometer

Over the past two decades, great efforts have been made toward mass spectrometer instrument miniaturization. With increasing analytical performances, miniature mass spectrometers are on the edge of being applied to more application scenarios. Besides sensitivity, mass resolution, and instrument portability, high-throughput and little or no sample preparation are also critical features in practical applications. In this study, we report the development of a miniature mass spectrometry (MS) system equipped with a 2D moving platform and a laser spray ionization (LSI) source. The method to make a patterned sample holder was also introduced and optimized for automatic high-throughput sample analyses. With the LSI source, analytes in complex matrix could be directly mass analyzed; in addition to the 2D moving platform, different samples could be analyzed in a high-throughput fashion. Results show that good linearity of quantitation could be achieved for multiple samples. Tens of nanograms of drugs, peptides, and vitamin B could be identified in diluted whole blood samples, and it takes 10 s on average to scan one sample.