Article
Food Science & Technology
Agustina Sarquis, Diellza Bajrami, Boris Mizaikoff, Victor Ladero, Miguel A. Alvarez, Maria Fernandez
Summary: The aim of this study was to investigate the biofilm-producing capacity of Lentilactobacillus parabuchneri strains. The results showed that the biofilm matrices of these strains were mainly composed of proteins and they easily adhered to beech wood. This study provides important insights into the biofilm-forming capacity of histamine-producing L. parabuchneri strains and how to prevent their formation for improving food safety.
Article
Chemistry, Applied
Natavan Hasanova, Saliha Esin Celik, Resat Apak
Summary: A simple and sensitive nanoparticle-based fluorometric method has been developed to determine the total content of biogenic amines in food samples. This method provides important information about the quality and safety of food products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Muhammad Abdurrahman Munir, Khairiah Haji Badri, Lee Yook Heng, Ahlam Inayatullah, Eva Nurinda, Daru Estiningsih, Annisa Fatmawati, Veriani Aprilia, Nur Syafitri
Summary: Histamine detection in fish mackerel is important for assessing allergic reactions and food freshness. This study developed a histamine sensor based on electrodes modified with polyurethane-LiClO4, which showed satisfactory selectivity and sensitivity. The sensor has great potential for analyzing histamine content in fish mackerel.
Article
Nutrition & Dietetics
Sonia Sanchez-Perez, Oriol Comas-Baste, M. Teresa Veciana-Nogues, M. Luz Latorre-Moratalla, M. Carmen Vidal-Carou
Summary: A low-histamine diet is currently the most advised strategy for preventing the symptoms of histamine intolerance. However, there is great heterogeneity in the type of excluded foods among different studies, with fermented foods being the only unanimous exclusion. Approximately 32% of excluded foods have high histamine content as a likely explanation.
Article
Chemistry, Analytical
Shuangshuang Yang, Jinya Du, Menglian Wei, Yan Huang, Yufan Zhang, Yeyu Wang, Jinze Li, Wei Wei, Yuchun Qiao, Haifeng Dong, Xueji Zhang
Summary: Rapid, simple, sensitive and reliable approaches for quantifying biogenic amines in food samples are essential for food safety. Here, the authors reported a Fe3O4@Au nanoparticles based multimodal readout LFIA for rapid quantification of putrescine and histamine. The assay showed high sensitivity and could be easily expanded to detect other biogenic amines.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Jaehi Kim, Min-Sup Shin, Jonghyun Shin, Hyung-Mo Kim, Xuan-Hung Pham, Seung-min Park, Dong-Eun Kim, Young Jun Kim, Bong-Hyun Jun
Summary: Rapid, accurate, and convenient diagnosis is crucial for effective disease management, and lateral flow immunoassay (LFIA) with optical nanoparticles (NPs) has emerged as a major diagnostic tool. Researchers have developed various types of optical NPs with modified optical properties to serve as probes for LFIA. This review focuses on the literature about LFIA with optical NPs for specific target detection in diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Applied
Guoru Li, Ragini Singh, Jiajun Guo, Bingyuan Zhang, Santosh Kumar
Summary: We fabricated Nb2CTx-enhanced localized surface plasmon resonance (LSPR) sensors using double S-tapered fiber sensors functionalized with gold nanoparticles/graphene oxide/tyrosinase and gold nanoparticles/Nb2CTx/tyrosinase. The Nb2CTx-enhanced LSPR sensor showed superior performance due to the presence of surface functional groups and large specific surface area of Nb2CTx. Our work provides a research platform for improving the sensitivity of LSPR sensors.
APPLIED PHYSICS LETTERS
(2023)
Review
Chemistry, Analytical
Bahar Ince, Mustafa Kemal Sezgintuerk
Summary: This article introduces the application of portable, disposable, and inexpensive lateral flow assay (LFA) systems in the diagnosis of infectious diseases. It first examines the design principles and working mechanisms of existing LFA methods and then presents current LFA implementation strategies for diseases such as COVID-19, Zika and dengue, HIV, hepatitis, influenza, malaria, and others. Furthermore, it summarizes the current problems and accessible solutions in detecting infectious agents and diseases by LFA, with a focus on increasing sensitivity through various detection methods. The article also envisions future trends in LFA-based diagnostics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Lucas Kettner, Ines Seitl, Lutz Fischer
Summary: A new diamine oxidase (DAO-1) was discovered in yeast, which has the potential to treat histamine intolerance by oral supplementation under simulated intestinal conditions.
Article
Food Science & Technology
D. Sylvain Dabade, Liesbeth Jacxsens, Lisa Miclotte, Emmanuel Abatih, Frank Devlieghere, Bruno De Meulenaer
Summary: This study quantitatively determined multiple biogenic amines in various food products sold in the Belgian market, and found significant positive correlations between BA concentrations and corresponding precursor FAAs, with stronger correlations in meat-based products. The study provides a basis for assessing the potential public health impact of BAs, which is currently limited to histamine in scombroid fish species and their derived products.
Article
Chemistry, Applied
Lin Luo, Shuang-Zi Luo, Bao-Zhu Jia, Wen-Feng Zhang, Hong Wang, Xiao-Qun Wei, Yu-Dong Shen, Zhen-Lin Xu, Jin-Yi Yang
Summary: In this work, a specific monoclonal antibody against tyramine was produced using a new hapten design. A high-resolution multicolor colorimetric immunoassay for tyramine was developed by integrating enzyme-induced multicolor generation with smartphone-assisted signal readout. The immunoassay showed a limit of detection of 19.7 mg/kg or L in beef, pork, and yogurt, and the results were reliable.
Review
Medicine, Research & Experimental
Xirui Chen, Lu Ding, Xiaolin Huang, Yonghua Xiong
Summary: This article introduces the application of engineered noble metal nanoparticles in improving immunoassay sensitivity. By modulating the physical parameters of nanoparticles, including size, shape, composition, and external structure, the performance of immunoassay can be improved. Engineered nanoparticles have a wide range of applications in trace analysis.
Article
Nutrition & Dietetics
Sonia Sanchez-Perez, Oriol Comas-Baste, Judit Costa-Catala, Irache Iduriaga-Platero, M. Teresa Veciana-Nogues, M. Carmen Vidal-Carou, M. Luz Latorre-Moratalla
Summary: There are uncertainties regarding the onset of histamine adverse effects that still require further research. This study investigated the interference of different amines on the rate of histamine degradation by the enzyme DAO. The results showed that certain amines significantly delayed histamine degradation, while others inhibited it, suggesting a possible explanation for the variable susceptibility to dietary histamine.
FRONTIERS IN NUTRITION
(2022)
Review
Chemistry, Applied
Beatriz del Rio, Maria Fernandez, Begona Redruello, Victor Ladero, Miguel A. Alvarez
Summary: Biogenic amines (BA) are molecules with biological functions that can accumulate at toxic concentrations in foods. Some microorganisms have been identified as responsible for their accumulation. Histamine, tyramine, and putrescine are the most commonly found BA at highest concentrations. Ingesting food with high BA concentrations can lead to intoxication. Additionally, there is synergy between different BA, emphasizing the need for regulation to protect consumer health.
Article
Biochemistry & Molecular Biology
Maria Salvador, Gemma Gutierrez, Sara Noriega, Amanda Moyano, Maria Carmen Blanco-Lopez, Maria Matos
Summary: Superparamagnetic magnetite nanoparticles with narrow size distribution have been successfully synthesized through precipitation in water-in-oil microemulsion, showing great potential in medical and environmental applications. The optimization of washing protocol and their applicability in bioapplications have been demonstrated. These novel materials are useful for analytical applications requiring high sensitivity and interference removal, as shown in signal enhancement and magnetic separation tests.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Izabell Craciunescu, Elena Chitanu, Mirela M. Codescu, N. Iacob, A. Kuncser, V. Kuncser, V. Socoliuc, Daniela Susan-Resiga, Florica Balanean, G. Ispas, Tunde Borbath, I. Borbath, Rodica Turcu, L. Vekas
Summary: High magnetization Fe3O4/OA-FeCo/Al2O3 nanocomposite magnetic clusters were successfully synthesized via a modified oil-in-water miniemulsion method. These clusters, with a combination of magnetite nanoparticles and FeCo/Al2O3 particles, exhibited enhanced magnetic and flow properties in ferrofluid carrier. The unique structure and composition of the Fe3O4/OA-FeCo/Al2O3 clusters contribute to their promising performance as a new type of nanocomposite magnetorheological fluid, as demonstrated by advanced characterization techniques.
Article
Chemistry, Multidisciplinary
Maria Salvador, Jose Luis Marques-Fernandez, Jose Carlos Martinez-Garcia, Dino Fiorani, Paolo Arosio, Matteo Avolio, Francesca Brero, Florica Balanean, Andrea Guerrini, Claudio Sangregorio, Vlad Socoliuc, Ladislau Vekas, Davide Peddis, Montserrat Rivas
Summary: Public health is a significant challenge in today's society, and early diagnosis and treatment of cancer are a priority. Magnetic nanoparticles have great potential in various biomedical applications. This study focuses on the polymeric coating of superparamagnetic iron oxide nanoparticles and analyzes their performance in resonance imaging, magnetic hyperthermia, and biosensing. Different types of fatty acid coatings were examined, and their characteristics were evaluated.
Article
Chemistry, Multidisciplinary
Maria Salvador, Jose Luis Marques-Fernandez, Alexander Bunge, Jose Carlos Martinez-Garcia, Rodica Turcu, Davide Peddis, Maria del Mar Garcia-Suarez, Maria Dolores Cima-Cabal, Montserrat Rivas
Summary: Lateral flow immunoassays combined with magnetic nanoclusters and antibodies, along with two strategies to increase sensitivity and accuracy, hold great promise for application in pneumonia diagnosis.
Article
Chemistry, Analytical
Baihui Wang, Amanda Moyano, Jose Maria Duque, Luis Sanchez, Guillermo Garcia-Santos, Luis J. Garcia Florez, Esther Serrano-Pertierra, Maria del Carmen Blanco-Lopez
Summary: This study developed a novel lateral flow immunoassay system based on Fe3O4 nanozymes for detecting extracellular vesicles (EVs).
Article
Pharmacology & Pharmacy
Verdiana Marchiano, Maria Matos, Ismael Marcet, Maria Paz Cabal, Gemma Gutierrez, Maria Carmen Blanco-Lopez
Summary: The eradication of bacterial infections has become more difficult due to bacterial resistance against traditional antibiotics. This study focuses on using drug delivery systems, specifically non-ionic vesicles, to combat bacterial infections by encapsulating the antibiotic Rifamycin S. The antimicrobial activity of Rifamycin S against Staphylococcus aureus was studied, and three formulations of niosomes were prepared using different aqueous solutions. The results showed that niosomes with a glycerol solution as the aqueous phase had higher stability and encapsulation efficiency, as well as a lower minimum inhibitory concentration (MIC) against S. aureus.
Article
Pharmacology & Pharmacy
Elena-Alina Moaca, Claudia Watz, Alexandra-Corina Faur, Daniela Lazar, Vlad Socoliuc, Cornelia Pacurariu, Robert Ianos, Cristiana-Iulia Rus, Daliana Minda, Lucian Barbu-Tudoran, Cristina Adriana Dehelean
Summary: The study successfully synthesized biocompatible magnetic iron oxide nanoparticles (MNPs) using ethanolic extracts from Camellia sinensis L. and Ocimum basilicum L. leaves. The MNPs obtained from Ocimum basilicum L. extract showed higher cell viability impairment on lung cancer cell lines (A549) compared to those obtained from Camellia sinensis L. extract. The biosafety evaluation using EpiAirwayTM models showed no significant decrease in viability or histopathological changes after treatment with the MNPs.
Article
Biochemistry & Molecular Biology
Verdiana Marchiano, Maria Matos, Miriam Lopez, Shihan Weng, Esther Serrano-Pertierra, Susana Luque, M. Carmen Blanco-Lopez, Gemma Gutierrez
Summary: Vanillin, a natural compound with various properties, was encapsulated in non-ionic surfactant vesicles. The vesicles were synthesized using thin film hydration and ethanol injection methods. The encapsulation efficiency and loading capacity of the vesicles were evaluated. Furthermore, vanillin-loaded vesicles were incorporated into gelatin films for potential food packing applications. The films were characterized in terms of morphology, water solubility, color, and transparency.
Article
Chemistry, Analytical
Shayesteh Bazsefidpar, Esther Serrano-Pertierra, Gemma Gutierrez, Alberto Sanchez Calvo, Maria Matos, Maria Carmen Blanco-Lopez
Summary: The study developed a sensitive lateral flow immunoassay (LFIA) for rapid detection of Escherichia coli (E. coli) O157:H7, a pathogen responsible for worldwide diseases and fatalities. Gold nanoparticles were synthesized and coated with a carboxylated heterobifunctional PEG polymer to be used as a label in sandwich-LFIA. A silver enhancement strategy was developed to amplify the signal, reducing the limit of detection (LOD). The system demonstrated improved sensitivity compared to the conventional LFIA system and showed effectiveness in quickly detecting pathogenic bacteria in food products.
Article
Chemistry, Analytical
Jose Luis Marques-Fernandez, Maria Salvador, Jose Carlos Martinez-Garcia, Pablo Fernandez-Miaja, Alfredo Garcia-Arribas, Montserrat Rivas
Summary: We demonstrate the use of resonant planar coils as sensors for reliable detection and quantification of magnetic nanoparticles. The coil's resonant frequency depends on the adjacent materials' magnetic permeability and electric permittivity. By dispersing a small number of nanoparticles on a supporting matrix on top of a planar coil circuit, the nanoparticles can be quantified. This nanoparticle detection has applications in biomedicine, food quality assurance, and environmental control challenges. We developed a mathematical model that accurately calculates the nanoparticles' mass from the self-resonance frequency of the coil at radio frequencies. The model relies only on the refraction index of the material around the coil, making it superior to other sensors that rely on separate magnetic permeability and electric permittivity measurements. The sensor can be scaled and automated for portable devices, providing a low-cost solution for measuring small quantities of nanoparticles. This resonant sensor, combined with the mathematical model, represents a significant improvement over other types of inductive sensors.
Article
Chemistry, Analytical
Shayesteh Bazsefidpar, Maria Freitas, Clara R. Pereira, Gemma Gutierrez, Esther Serrano-Pertierra, Henri P. A. Nouws, Maria Matos, Cristina Delerue-Matos, Maria Carmen Blanco-Lopez
Summary: In this paper, a method for effectively controlling Escherichia coli (E. coli) O157:H7 in milk samples was developed. Monodisperse Fe3O4@Au magnetic nanoparticles were synthesized and used in an electrochemical sandwich-type magnetic immunoassay for rapid and accurate analysis. The assay showed a linear range from 20 to 2 x 10(6) CFU/mL, with a limit of detection of 20 CFU/mL for E. coli O157:H7. The selectivity of the assay was tested and the applicability was demonstrated by analyzing a commercial milk sample.
Article
Chemistry, Inorganic & Nuclear
Maria-Cristina Ioncica, Sulalit Bandyopadhyay, Nesrine Bali, Vlad Socoliuc, Sandor I. Bernad
Summary: Based on prior research, iron-oxide nanoparticles (IONPs) of spherical and cubic shapes were synthesized and sized for this study. The magnetic, rheological, and sedimentation properties of PEG-coated IONP suspensions were investigated for potential magnetic targeting applications. The results showed similar shear viscosity behavior of both types of suspensions under different magnetic fields, with shear thinning observed at low shear rates. The study also revealed that spherical IONPs exhibited a higher viscosity at lower shear rates and demonstrated more significant fluctuation in hydrodynamic diameter compared to cubic IONPs. Additionally, cubic-shaped IONPs exhibited greater stability and less tendency to sediment than their spherical counterparts.
Review
Chemistry, Multidisciplinary
V Socoliuc, M. Avdeev, V Kuncser, Rodica Turcu, Etelka Tombacz, L. Vekas
Summary: Ferrofluids are colloidal suspensions of magnetic nanoparticles with fluid and magnetic properties, which have been investigated for over five decades. They have proven to be highly controllable and tunable, making them suitable for a wide range of engineering applications. Recently, biocompatible ferrofluids have attracted significant interest for their potential applications in nanomedicine, biotechnology, and environmental protection. This paper provides an overview of early results, as well as recent advancements in ferrofluid synthesis, characterization, ferrohydrodynamics, and applications in sensors, actuators, and droplet technology.