Article
Biology
Ibrahim Al-Ashkar, Walid Ben Romdhane, Rania A. El-Said, Abdelhalim Ghazy, Kotb Attia, Abdullah Al-Doss
Summary: The study evaluated the salt tolerance potential of four doubled haploid lines of wheat and compared them with the check cultivar using an extensive set of agro-physiologic parameters and salt-stress-related gene expressions. The results indicated that the five genotypes tested displayed reduction in all traits evaluated except the canopy temperature and electrical conductivity, with DHL2 exhibiting the least decline. Genotypes DHL21 and DHL5 showed increased expression rate of salt-stress-related genes under salt stress conditions, and identified unbiased traits for assessing wheat DHLs under salinity conditions.
Review
Biotechnology & Applied Microbiology
Serik Eliby, Sara Bekkuzhina, Olena Kishchenko, Gulnur Iskakova, Gulnar Kylyshbayeva, Satyvaldy Jatayev, Kathleen Soole, Peter Langridge, Nikolai Borisjuk, Yuri Shavrukov
Summary: Doubled haploid production is a valuable biotechnology that accelerates the breeding of new wheat varieties and plays important roles in studying genetic control, marker-assisted selection, genomics, and genetic engineering in wheat. This paper reviews recent advances in androgenesis and gynogenesis techniques, with a focus on the in vitro culture phase, and the emerging innovative approaches in researching and producing wheat doubled haploids. The use of CRISPR-based genome editing for inducing doubled haploids in wheat is also discussed.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Haixiao Hu, Yujie Meng, Wenxin Liu, Shaojiang Chen, Daniel. E. E. Runcie
Summary: Recent advances in maize DH technology enable the efficient development of large numbers of DH lines. Phenotyping of haploid progenitors can accelerate the selection of DH lines. Multi-trait models improve genomic prediction accuracy for DH lines, but incorporating information from haploid lines does not further improve the prediction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Rabouan Oubaidou, Samira Hentour, Chafika Houasli, Rachid Aboutayeb, Youness El Goumi, Ezzouhra El Maaiden, Fatima Gaboun, Ouafa Lamsaouri, Malika Fakiri
Summary: Salt stress is a major environmental factor affecting cereal productivity, and selecting salt tolerant genotypes is an effective way to overcome yield losses. This study compared phenotypic variability among doubled haploid barley lines and their parent under different salinity conditions. Results showed that traits like plant height and number of grains per spike play significant roles in determining grain yield, with number of fertile spikes per plant and number of grains per spike being indicative of salt tolerance in barley.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2021)
Article
Agronomy
Thobeka Philile Khumalo, Tsepiso Hlongoane, Annelie Barnard, Toi John Tsilo
Summary: The study evaluated wheat populations in South Africa to identify genotypes with increased preharvest sprouting tolerance. The findings identified specific loci associated with tolerance and recommended genotypes for further breeding efforts. These results will contribute to the development of PHS-tolerant cultivars in South Africa.
Article
Plant Sciences
Surbhi Grewal, Veronica Guwela, Claire Newell, Cai-yun Yang, Stephen Ashling, Duncan Scholefield, Stella Hubbart-Edwards, Amanda Burridge, Alex Stride, Ian P. King, Julie King
Summary: This study generated 86 stable inherited doubled haploid (DH) wheat-T. urartu introgression lines, which were characterized using genotyping arrays and KASP markers to identify unique introgressions in a bread wheat background. The DH lines have accelerated the breeding process and enabled the early release of homozygous wheat-T. urartu introgression lines, which can greatly advance the identification of beneficial alleles for wheat improvement.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Muhammad Fuad Anshori, Bambang Sapta Purwoko, Iswari Saraswati Dewi, Sintho Wahyuning Ardie, Willy Bayuardi Suwarno
Summary: By utilizing a multivariate analysis approach, this study established the indirect selection index of doubled haploid rice to select lines adaptive to salinity stress. Through field and greenhouse experiments, a total of 28 DH rice lines were identified as adaptive to salinity stress based on good agronomic traits and salinity tolerance. The effectiveness of the indirect adaptability selection index was validated through field experiments in a saline area.
Article
Agronomy
Xuelian Li, Xiuhao Quan, Melissa Mang, Guenter Neumann, Albrecht Melchinger, Uwe Ludewig
Summary: Research shows that modern elite maize varieties may exhibit trends of decreased AMF colonization or changes in organic acid anion release under low P supply, but overall, there is no evidence to suggest the loss of beneficial traits for the flint pool of maize.
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
(2021)
Article
Plant Sciences
Long Li, Zhi Peng, Xinguo Mao, Jingyi Wang, Chaonan Li, Xiaoping Chang, Ruilian Jing
Summary: Developing salt-tolerant crop varieties is crucial to combat soil salinization globally. This study identified novel genes and genetic loci associated with salt responsiveness in wheat, while also highlighting the decline in salt tolerance among Chinese wheat varieties. The findings suggest the importance of considering genetic diversity and selection pressures in breeding for salt tolerance.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biology
Muniyandi Samuel Jeberson, Harinder Kumar Chaudhary, Rakesh Kumar Chahota, Shabir Hussain Wani
Summary: The study explores the potential of improving bread wheat using the rye genome, with wheat-rye substitutions and translocations being commonly utilized for resistance breeding. Crosses of different generations were used to assess various haploid induction parameters, and molecular cytogenetic analysis revealed an IBL.1RS translocation and substitution of the 5R chromosome in doubled haploids from BC2F3.
Article
Agronomy
Muhammad Bilawal Junaid, Salah El-Hendawy, Ibrahim Al-Ashkar, Nasser Al-Suhaibani, Majed Alotaibi
Summary: By using various analysis tools and molecular markers, we successfully assessed the salt tolerance of wheat genotypes under real field conditions. The results revealed significant differences in salt tolerance among different genotypes, and certain traits were found to be closely associated with the salt tolerance of genotypes. Through heatmap cluster analysis and SSR markers, we categorized the genotypes into different clusters and identified key genes related to salt tolerance.
Article
Plant Sciences
Salah El-Hendawy, Nasser Al-Suhaibani, Muhammad Mubushar, Muhammad Usman Tahir, Yahya Refay, ElKamil Tola
Summary: The study compared the relative performance of GY for spring wheat germplasm under different conditions and evaluated a hyperspectral reflectance tool for early estimation of GY and STIs. Significant genotypic variations and relationships between SRIs and GYc, STI, and GMP were observed, indicating the potential of using SRIs for accurate estimation of genotype performance under varying environmental conditions. This highlights the importance of incorporating STIs with early estimation of GY for successful genotype development under stress conditions.
Article
Agronomy
Iara Goncalves dos Santos, Anderson Luiz Verzegnazzi, Jode Edwards, Ursula K. Frei, Nicholas Boerman, Leandro Tonello Zuffo, Luiz P. M. Pires, Gerald de la Fuente, Thomas Lubberstedt
Summary: This study demonstrates that spontaneous haploid genome doubling does not affect the performance of inbred lines and that BS39 genes have the potential to add novel alleles to temperate elite germplasm.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Agronomy
Jian Wang, Yuexuan Cao, Kejian Wang, Chaolei Liu
Summary: The panel of multiple-heading-date mtl haploid inducer lines developed in this study can address the issue of asynchronous heading dates between haploid inducers and pollen acceptors, accelerating the use of in vivo DH technology in rice breeding.
Article
Genetics & Heredity
Muhammad Massub Tehseen, Fatma Aykut Tonk, Muzaffer Tosun, Harpinder Singh Randhawa, Ezgi Kurtulus, Izzet Ozseven, Behyan Akin, Ozge Nur Zulfuagaoglu, Kumarse Nazari
Summary: This study identified multiple QTLs controlling stripe rust resistance in wheat using an elite line with adult plant resistance. SNP markers flanking the QTL regions can aid in marker-assisted selection.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Mohammad Rafiqul Islam, Mohd Mostofa Kamal, Mohammad Faruk Hossain, Jamil Hossain, Mohammad Golam Azam, Mst Masuma Akhter, M. Kamrul Hasan, Ibrahim Al-Ashkar, Khalid F. Almutairi, Ayman EL Sabagh, Md Atikur Rahman, Muhammad Aamir Iqbal, Mohammad Sohidul Islam
Summary: The study investigated the physiological and biochemical responses of different mung bean genotypes under well-watered and water-deficit conditions, finding that drought-tolerant genotypes showed better physiobiochemical attributes and adaptability. Among them, BMX-08010-2 exhibited the highest superiority in terms of physio-biochemical traits, susceptibility index, and stress tolerance index, making it a suitable candidate for sustaining yield under drought stress and potential for crop improvement through hybridization programs.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Geosciences, Multidisciplinary
Aqil Tariq, Yan Jiango, Linlin Lu, Ahsan Jamil, Ibrahim Al-ashkar, Muhammad Kamran, Ayman El Sabagh
Summary: This research compares the use of SAR and Optical sensors in identifying and mapping burnt and unburnt scars during bushfires in Australia and Pakistan. The study found that both SAR and optical remote sensing data could effectively identify and measure the impacts of forest fires.
GEOMATICS NATURAL HAZARDS & RISK
(2023)
Article
Environmental Sciences
Khalid Maniah, Islam Nour, Atif Hanif, Mohamed Taha Yassin, Abdulrahman Alkathiri, Ibrahim Al-Ashkar, Saleh Eifan
Summary: Regular water environment monitoring is crucial for minimizing contamination caused by waterborne viruses and reducing health risks. The study detected human adenoviruses in three wastewater treatment plants in Riyadh, Saudi Arabia, and investigated the impact of seasonal variability and meteorological factors on the prevalence of HAdVs.
Article
Multidisciplinary Sciences
Hamada M. Hassan, Adel A. Hadifa, Sara A. El-leithy, Maria Batool, Ahmed Sherif, Ibrahim Al-Ashkar, Akihiro Ueda, Md Atikur Rahman, Mohammad Anwar Hossain, Ayman Elsabagh
Summary: Plant hybridization is essential for breeding new varieties with desired traits. This study aimed to evaluate gene action, heritability, and heterosis of various traits in rice under water deficit conditions. Crossing four different water-deficit tolerant rice genotypes produced populations with improved performances and adaptability to water deficit condition. The study also identified different types of gene interactions influencing the inheritance of specific traits. The findings provide valuable insights for future phenotypic and genotypic association studies in rice and for developing water deficit tolerant rice cultivars.
Article
Agronomy
Bushra Ahmed Alhammad, Mahmoud F. Seleiman
Summary: This study explores environmentally friendly approaches to enhance crop growth and productivity. The results suggest that the combination of biogas digestate and bio-fertilizer can improve the growth, yield, and quality of faba bean.
Article
Agronomy
Ibrahim Al-Ashkar, Mohammed Sallam, Khalid F. Almutairi, Mohamed Shady, Abdullah Ibrahim, Salem S. Alghamdi
Summary: Abiotic stress reduces crop production worldwide. To recommend suitable genotypes for cultivation under water deficit and heat stress conditions, understanding the genetic basis and plant responses to these stresses and their interactions with the environment is crucial. In this study, the MGIDI index was used to identify abiotic-stress-tolerant wheat genotypes, while the WAASB and WAASBY indices were used to recognize high-yielding and stable genotypes. Twenty wheat genotypes were evaluated for their abiotic stress tolerance under various test conditions, and certain genotypes showed slight reductions in measured traits compared to the control group. The genotypes G12 and G17 were found to be the most stable candidates based on different indices, indicating their potential for improving productivity and stabilizing grain yield under optimal, water deficit, and heat stress conditions.
Article
Agronomy
Bushra Ahmed Alhammad, Awais Ahmad, Mahmoud F. F. Seleiman
Summary: Heavy metal stress, particularly lead stress, negatively affects the growth, physiology, and yield of plants. However, the combined application of zinc oxide nanoparticles (50 mg L-1) and nano-hydroxyapatite (50 mg kg(-1)) can alleviate the adverse effects of lead stress and improve the growth and yield of maize. The combined treatment also enhanced the uptake of nutrients and reduced the uptake of lead in plants.
Article
Agronomy
Elsayed Mansour, El-Sayed E. A. El-Sobky, Mohamed I. E. Abdul-Hamid, Eman Abdallah, Abdeltawab M. I. Zedan, Ahmed M. Serag, Cristina Silvar, Salah El-Hendawy, El-Sayed M. Desoky
Summary: The present study aimed to explore the influence of exogenously sprayed moringa seed extract and α-tocopherol on physio-biochemical, morphological, and yield attributes of six diverse maize hybrids under three irrigation levels in poor-fertility sandy soil. The results showed that foliar application of moringa seed extract or α-tocopherol enhanced maize tolerance to drought stress by increasing the accumulation of osmoprotectants, improving antioxidant enzymes, and reducing membrane lipid peroxidation and electrolyte leakage, resulting in improved yield traits and crop water productivity under water deficit conditions.
Article
Agronomy
Nahid A. A. Morsi, Omnia S. M. Hashem, Mohamed A. Abd El-Hady, Yasser M. M. Abd-Elkrem, Mohamed E. E. El-temsah, Elhussin G. G. Galal, Khaled I. I. Gad, Ridha Boudiar, Cristina Silvar, Salah El-Hendawy, Elsayed Mansour, Mohamed A. A. Abdelkader
Summary: Drought is a major abiotic stress that affects canola growth, production, and quality. Developing drought-tolerant and high-yielding canola genotypes is critical for sustaining production and ensuring global food security. This study evaluated ten canola genotypes under different irrigation regimes and found that certain tissue-cultured and exotic genotypes performed better than commercial cultivars under water-deficit conditions.
Article
Agronomy
Mahmoud F. Seleiman, Awais Ahmad, Thobayet S. Alshahrani
Summary: Salinity poses a major challenge to agricultural development and food safety. This study investigated the effects of root application of zinc oxide nanoparticles (ZnO-NPs) and 24-epibrassinolide (EBL) on the growth and performance of maize under salt stress. The results showed that the combined treatment of ZnO-NPs and EBL effectively mitigated salt stress and improved the growth and physiological performance of maize.
Article
Microbiology
Saleh Eifan, Khalid Maniah, Islam Nour, Atif Hanif, Mohamed Taha Yassin, Ibrahim Al-Ashkar, Islem Abid
Summary: In Saudi Arabia, the concentration of Pepper mild mottle virus (PMMoV) and human adenovirus (HAdV) in wastewater treatment plants was measured. PMMoV was found in 94% of wastewater samples with concentrations ranging from 62 to 3.5 x 10(7) genome copies/l, while HAdV was detected in 75% of raw water samples with concentrations ranging from 1.29 x 10(3) GC/L to 1.26 x 10(7) GC/L. A higher positive correlation between PMMoV and HAdV concentrations was observed at one of the treatment plants.
Article
Chemistry, Physical
Bushra Ahmed Alhammad, Aftab Jamal, Claudia Carlucci, Muhammad Farhan Saeed, Mahmoud F. Seleiman, Marcelo F. Pompelli
Summary: Renewable energy sources have become a global concern due to global warming. Biofuels can reduce greenhouse gas emissions and change the energy landscape. This study found that non-edible plant species have biofuel-like attributes, and there are distinct differences in fatty acid profiles among different plant species. The findings demonstrate the potential of non-conventional oilseeds in sustainable biofuel production.
Article
Plant Sciences
Ibrahim Al-Ashkar, Mohammed Sallam, Abdullah Ibrahim, Abdelhalim Ghazy, Nasser Al-Suhaibani, Walid Ben Romdhane, Abdullah Al-Doss
Summary: Multiple abiotic stresses negatively affect wheat production. This study used multivariate analysis techniques to evaluate the performance of 20 wheat genotypes under multiple abiotic stresses and identified stable and stress-tolerant genotypes.
Article
Chemistry, Multidisciplinary
Mohd Salim Mir, Parmeet Singh, Tauseef A. Bhat, Raihana H. Kanth, Aijaz Nazir, Ibrahim Al-Ashkar, Muhammad Kamran, Adel A. Hadifa, Ayman El Sabagh
Summary: Direct drum seeding is a viable alternative to transplanted rice, addressing labor and water scarcity issues. However, weed infestation is a major concern, necessitating efficient weed management practices. An experiment in India showed that early sowing and mechanized conoweeding or sequential herbicide application resulted in higher yields and effective weed control.
Article
Agronomy
Muhammad Bilawal Junaid, Salah El-Hendawy, Ibrahim Al-Ashkar, Nasser Al-Suhaibani, Majed Alotaibi
Summary: By using various analysis tools and molecular markers, we successfully assessed the salt tolerance of wheat genotypes under real field conditions. The results revealed significant differences in salt tolerance among different genotypes, and certain traits were found to be closely associated with the salt tolerance of genotypes. Through heatmap cluster analysis and SSR markers, we categorized the genotypes into different clusters and identified key genes related to salt tolerance.