Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
B. Linju Yen, Ko-Jiunn Liu, Huey-Kang Sytwu, Men-Luh Yen
Summary: In the past two decades, the use of mesenchymal stem cells (MSCs) in clinical trials has increased significantly. However, therapeutic success has not met expectations, possibly due to donor-specific differences and the availability of multiple tissue/organ sources for MSC isolation. This review summarizes past findings and focuses on comparing MSCs isolated from the commonly used sources of bone marrow, adipose tissue, and post-partum discarded extraembryonic tissue. The functional differences between MSC sources, including differentiation capacity, hematopoietic support, immunomodulation, and paracrine capacities, are discussed. The implications of these tissue-specific functional differences on future research, manufacturing practices, and clinical implementation are also discussed.
Review
Biochemistry & Molecular Biology
Yujie Song, Christian Jorgensen
Summary: This review highlights the potential of mesenchymal stromal cells (MSCs) in treating osteoarthritis (OA), focusing on their chondrogenic and immunomodulatory potential. The review provides an overview of the effects of MSCs in preclinical and clinical studies of OA treatment, comparing different MSC sources. Furthermore, the review summarizes the current evidence for the assessment of cartilage in OA using magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy.
Article
Cell Biology
Suchi Gupta, Sonali Rawat, Vishnu Krishnakumar, E. Pranshu Rao, Sujata Mohanty
Summary: Mesenchymal stromal cells (MSCs) are ideal candidates for regenerative medicine. This study investigated the effect of hypoxia exposure on tissue-specific MSCs. It was observed that hypoxia exposure enhanced the expression of immunomodulatory factors and increased exosome secretion in MSCs. However, different MSC types responded differently to hypoxia.
CELL AND TISSUE RESEARCH
(2022)
Article
Cell Biology
Domenico Aprile, Nicola Alessio, Ibrahim H. Demirsoy, Tiziana Squillaro, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisi
Summary: The study demonstrated that multi-lineage stem cells can be isolated from a mouse model, offering a valid alternative for disease research and cell therapy, and showing potential applications for these cells.
Review
Pharmacology & Pharmacy
Dragan Primorac, Martin Cemerin, Vid Matisic, Vilim Molnar, Marko Strbad, Lenart Girandon, Lucija Zenic, Miomir Knezevic, Stephen Minger, Denis Polancec
Summary: The COVID-19 pandemic has had a significant impact globally and the overaggressive immune reaction in response to the virus can lead to ARDS. Mesenchymal stromal cell therapy presents a potential treatment option for severe patients.
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Immunology
Fulvio Massaro, Florent Corrillon, Basile Stamatopoulos, Nathan Dubois, Achille Ruer, Nathalie Meuleman, Dominique Bron, Laurence Lagneaux
Summary: Mesenchymal stromal cells (MSC) play a critical role in aging and inflammaging, and studying them is important for understanding the mechanisms of diseases in the elderly.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Pharmacology & Pharmacy
Marta Dymowska, Aleksandra Aksamit, Katarzyna Zielniok, Monika Kniotek, Beata Kaleta, Aleksander Roszczyk, Michal Zych, Filip Dabrowski, Leszek Paczek, Anna Burdzinska
Summary: Although both BM-MSCs and WJ-MSCs have the ability to interact reciprocally with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings. WJ-MSCs secreted significantly more IL-6 than BM-MSCs and showed higher responsiveness to M1-derived chemotactic signals.
Article
Respiratory System
Dennis Kruk, Anna C. Y. Yeung, Alen Faiz, Nick H. T. ten Hacken, Wim Timens, Toin H. van Kuppevelt, Willeke Daamen, Danique Hof, Martin C. Harmsen, Mauricio Rojas, Irene H. Heijink
Summary: This study evaluated the transcriptome differences between lung-derived MSCs (LMSCs), bone marrow-derived MSCs (BM-MSC), and adipose-derived MSCs (AD-MSCs) from COPD patients and non-COPD controls. The results showed strong gene signature differences between MSCs from different sources. LMSCs predominantly expressed FGF10 and HGF growth factors. MSCs from all sources displayed altered gene expression in COPD, with the most significant changes observed in AD-MSCs, particularly in genes involved in extracellular matrix binding and expression. The gene CSGALNACT1 showed the strongest difference between COPD and control in LMSCs, which is a gene involved in extracellular matrix modulation.
RESPIRATORY RESEARCH
(2023)
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Oncology
Gerardo-Javier Marti-Chillon, Sandra Muntion, Silvia Preciado, Lika Osugui, Almudena Navarro-Bailon, Javier Gonzalez-Robledo, Victor Sagredo, Juan F. Blanco, Fermin Sanchez-Guijo
Summary: Despite advancements in ICU patient care, the management of those with SIRS is still a medical challenge. The hyperinflammatory state caused by initial injury and uncontrolled response leads to systemic hypotension and organ failure. MSCs, with their immunomodulatory and regenerative properties, are being explored as a potential therapy for SIRS.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Review
Oncology
Bushu Harna, Pulkit Kalra, Shivali Arya, Naveen Jeyaraman, Arulkumar Nallakumarasamy, Madhan Jeyaraman, Ramya Lakshmi Rajendran, Eun Jung Oh, Manish Khanna, Uma Maheswari Rajendran, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: The management of relapses and refractory rheumatoid arthritis (RA) patients is complex and difficult. There are serious side effects and drug interactions with disease-modifying anti-rheumatic drugs (DMARDs), and some subsets of RA patients do not respond to DMARDs. Mesenchymal stem cells (MSCs) provide a promising alternative for RA treatment, with the potential to manage refractory cases not responding to conventional treatment.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Ben Antebi, Kerfoot P. Walker, Arezoo Mohammadipoor, Luis A. Rodriguez, Robbie K. Montgomery, Andriy I. Batchinsky, Leopoldo C. Cancio
STEM CELL RESEARCH & THERAPY
(2018)
Article
Cell & Tissue Engineering
Ben Antebi, Luis A. Rodriguez, Kerfoot P. Walker, Amber M. Asher, Robin M. Kamucheka, Lucero Alvarado, Arezoo Mohammadipoor, Leopoldo C. Cancio
STEM CELL RESEARCH & THERAPY
(2018)
Article
Cell & Tissue Engineering
Ben Antebi, Kerfoot P. Walker, Arezoo Mohammadipoor, Luis A. Rodriguez, Robbie K. Moore, Leopoldo C. Cancio, Andriy I. Batchinsky
REGENERATIVE MEDICINE
(2019)
Article
Cell & Tissue Engineering
Amy L. Xu, Luis A. Rodriguez, Kerfoot P. Walker, Arezoo Mohammadipoor, Robin M. Kamucheka, Leopoldo C. Cancio, Andriy Batchinsky, Ben Antebi
STEM CELLS TRANSLATIONAL MEDICINE
(2019)
Article
Medicine, Research & Experimental
Ben Antebi, Amber M. Asher, Luis A. Rodriguez, Robbie K. Moore, Arezoo Mohammadipoor, Leopoldo C. Cancio
JOURNAL OF TRANSLATIONAL MEDICINE
(2019)
Article
Ophthalmology
Sumin Gu, Sondip Biswas, Luis Rodriguez, Zhen Li, Yuting Li, Manuel A. Riquelme, Wen Shi, Ke Wang, Thomas W. White, Matthew Reilly, Woo-Kuen Lo, Jean X. Jiang
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)