期刊
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
卷 52, 期 4, 页码 347-357出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0003683816040128
关键词
whole-cell bacterial biosensors; reporter genes; gene fusion; aromatic hydrocarbons; polychlorinated biphenyls; environmental monitoring
资金
- Ministry of Education and Science of the Russian Federation [14.574.21.0028, RFMEFI57414X0028]
The review summarizes the data on new directions in biosensor technologies based on whole bacterial cells. Biosensors for the monitoring of mono(poly)aromatic hydrocarbons and their chlorinated derivatives, which are constructed with genetically modified bacterial cells bearing a reporter gene fusion, are considered. The operating principle of these biosensors is based on the expression of reporter genes (luc, lux, gfp, rfp) under the control of a promoter and a regulator that specifically respond to a detected compound.
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