期刊
JOURNAL OF CLINICAL MEDICINE
卷 8, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/jcm8020185
关键词
Vascular tissue engineering; umbilical cord blood; human cord blood-derived endothelial cells; endothelialization; vascular graft
资金
- NIH [1R56HL136517-01]
- Cellular Approaches to Tissue Engineering and Regeneration training grant (CATER) at the University of Pittsburgh [5T32EB001026]
- NSF [1337371]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1337371] Funding Source: National Science Foundation
Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.
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