Article
Biology
Wang-Soo Lee, Woojin Ham, Jaetaek Kim
Summary: NQO1 is an antioxidant flavoprotein that exerts antioxidant activities, anti-inflammatory effects, and interacts with tumor suppressors. Recent studies have highlighted the promising roles of NQO1 in protecting against cardiovascular damage and related diseases.
Article
Nanoscience & Nanotechnology
Haili Zhang, Xiao Xu, Dan Yan, Chunlin Ren, Jinghan Zhang, Mengzhen Gu, Yun Wang, Peiye Wu, Zhongrui Li, Lingyi Kong, Chao Han
Summary: Researchers developed a new nanodrug delivery system (PN) to increase ROS production by degrading the NQO1 protein, leading to enhanced anti-tumor effects. In vitro and in vivo studies demonstrated that PN significantly increased ROS production and exhibited potent antitumor properties. This nanoplatform might provide an alternative approach for treating tumors with NQO1 overexpression.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Engineering, Chemical
Xuewen Mu, Yun Xu, Zheng Wang, Dunyun Shi
Summary: NAD(P)H:quinone oxidoreductase 1 is an enzyme highly expressed in tumors and can be targeted for cancer therapy. Quinone-based bioimaging probes and nanosystems can be used for tumor diagnosis and treatment, improving effectiveness and reducing side effects.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2023)
Article
Chemistry, Medicinal
Kuojun Zhang, Kaizhen Wang, Xiangyu Zhang, Zhenlong Qian, Wenbo Zhang, Xiao Zheng, Jiaying Wang, Yin Jiang, Wanheng Zhang, Zhiyu Lu, Haiping Hao, Sheng Jiang
Summary: Targeting NAD(+) metabolism is an effective anticancer strategy. Small molecules that simultaneously target NQO1 and NAMPT were discovered, and one of these compounds, 10d, showed excellent in vivo antitumor efficacy without significant toxicity.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Guo Li, Yongxiao Xu, Xiaojie Kong, Liuwei Gu, Yuling Qin, Li Wu
Summary: An NQO1-responsive precursor called R848-QPA has been developed to induce an anti-tumor immune response. When activated by overexpressed NQO1 in the tumor microenvironment, R848-QPA can activate innate immunity, but it shows lower activity in NQO1-deprived environments. This strategy offers a new approach for the development of tumor-microenvironment-responsive prodrugs for antitumor immunotherapy.
CHEMICAL COMMUNICATIONS
(2023)
Review
Cell Biology
Daniel C. Levine, Kathryn M. Ramsey, Joseph Bass
Summary: Intrinsic circadian clocks regulate daily anticipation of light/dark cycles and energy storage/utilization cycles. Disruption of the core clock can contribute to disease. Peripheral clocks can be influenced by metabolic cues. NAD(P)(H) nucleotides and NAD+-dependent sirtuin deacetylases play a key role in integrating circadian and metabolic cycles.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Mamta, Y. Singh, K. K. Maurya, V. N. Singh
Summary: Sb2Se3, a p-type semiconductor, is widely used in thin film solar cells as an absorber layer. In this study, Sb2Se3 was prepared using the solid-state reaction route and thin films were deposited through thermal evaporation and annealing. The crystallinity of the thin films improved after annealing. The structural and optical properties of the material and thin films were characterized using XRD, Raman, SEM, and UV-Vis spectroscopy. A simple device structure was proposed and simulations based on Sb2Se3/c-Si hetero-junction photovoltaic cells were conducted. The simulations extracted parameters such as back contact work function, electron affinity, and layer thickness to optimize the device performance. The p-Sb2Se3 with a band gap of 1.64 eV achieved a maximum efficiency of 12.75% under optimized conditions. This simple device structure can have various local applications due to the availability of n-Si. It can also serve as a reliable method for predicting photovoltaic performance and exploring new tandem solar cell structures using Sb2Se3.
MATERIALS TODAY SUSTAINABILITY
(2022)
Article
Nutrition & Dietetics
Midori Nagase, Miku Sakamoto, Sakiko Amekura, Sayaka Akiba, Misato Kashiba, Kenji Yokoyama, Yorihiro Yamamoto, Akio Fujisawa
Summary: NQO is an important enzyme that protects living organisms and cells from oxidative stress. In this study, coenzyme Q10 reducing activity was found in fractions of rat liver homogenate. The compound responsible for the reduction was identified as riboflavin, which can also reduce other flavin and vitamin K compounds. This flavin-dependent CoQ10 reduction may function in aqueous phases and in the presence of saposin B.
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2023)
Article
Chemistry, Analytical
Han Wei, Yifang Yu, Guanghua Wu, Yueqin Wang, Shuyi Duan, Jinyan Han, Weiyan Cheng, Cai Li, Xin Tian, Xiaojian Zhang
Summary: The dual-responsive fluorescent probe 3Q-2 can reveal dynamic changes in NAD(P)H and mitochondrial viscosity during ferroptosis, a newly discovered form of cell death. This probe displays two separate spectra in response to NAD(P)H and mitochondrial viscosity with high sensitivity and selectivity. Cancer cells exhibit distinct levels of NAD(P)H and mitochondrial viscosity during ferroptosis induced by specific inhibitors. This robust method of detecting NAD(P)H and mitochondrial viscosity simultaneously may find applications in various physiological and pathological scenarios.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Jie Zhang, Zengchen Liu, Fengshou Tian, Yahong Chen
Summary: A fluorescent compound derived from coumarin and hemicyanine was synthesized and characterized in this paper, showing higher ratiometric fluorescence response activity toward NAD(P)H compared to other commonly coexisting compounds in the cell microenvironment. The fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H. This ratiometric fluorescent probe provides a theoretical basis for sensing NAD(P)H in vitro and in vivo, and was used to build a sensitive detection platform of NAD(P)H-dependent enzyme activity based on the fluorescence method.
ANALYTICAL METHODS
(2021)
Article
Biology
Anamika Battu, Rajaram Purushotham, Rupinder Kaur
Summary: Fld-LPs are important components of the oxidative stress defense system in organisms, with the ability to convert quinones to hydroquinones using NAD(P)H as an electron donor. The activity of Fld-LPs can be measured in whole cell extracts and their assembly and activity can be regulated by posttranslational modifications. This assay provides a reliable method to study fungal oxidative stress response systems and screen inhibitors specific for fungal Fld-LPs.
Article
Biochemistry & Molecular Biology
Anna V. Izosimova, Marina V. Shirmanova, Vladislav I. Shcheslavskiy, Daria A. Sachkova, Artem M. Mozherov, George V. Sharonov, Elena V. Zagaynova, Diana V. Yuzhakova
Summary: FLIM technology can be used to assess the immune response and metabolic changes of T-cells to tumors. The study found that the fluorescence lifetime parameters of NAD(P)H in lymph nodes are sensitive to tumor development. This research provides a powerful tool for evaluating the immune response to tumor development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Oleg S. Sutormin, Elena V. Nemtseva, Dmitry V. Gulnov, Lev A. Sukovatyi, Yekaterina S. Tyrtyshnaya, Albert E. Lisitsa, Valentina A. Kratasyuk
Summary: This study aims to investigate the effects of viscous medium on the kinetic features of NAD(P)H:FMN-oxidoreductase from luminous bacteria (Red) in both a single enzyme assay and in coupling with bacterial luciferase (BLuc). Varying concentrations of glycerol and sucrose were used to alter the medium viscosity. The study found that the presence of BLuc increased Red activity at low concentrations of glycerol, while Red activity was suppressed in viscous medium without BLuc. The interaction between glycerol/sucrose and Red substrates was also investigated using absorption spectroscopy and molecular dynamics.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Article
Engineering, Environmental
Yige Xiong, Xiaohui Yan, Taibai Li, Huixin Jin, Ziliang Chen, Xuejiao Xu, Xiang Ji, Xiang Ge
Summary: In this study, a new design strategy called Micro-tabless-pouch-cell(MTPC) is developed to achieve miniaturization of batteries. By replacing the conventional ultrasonic welding protocol with an innovative tab-glue-hot-welding process during sealing, MTPC demonstrates comparable electrochemical performance to coin cells while significantly reducing the weight of the entire device. MTPC also enables easy integration on flexible substrates and has a unique structure that allows for function extensibility, making it a promising option for various high-end flexible electronics.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Oncology
Bowen Weng, Xiaoyue Zhang, Xiaoyun Chu, Xiaohui Gong, Cheng Cai
Summary: The Nrf2-Keap1-antioxidant response element-NQO1 signaling pathway plays a protective role in hyperoxia-induced lung injury by inhibiting apoptosis, as demonstrated by the upregulation of Nrf2, Keap1, and NQO1 expression under hyperoxic conditions, and the downregulation of these genes leading to increased apoptosis after transfection with Nrf2 siRNA.
MOLECULAR MEDICINE REPORTS
(2021)
Review
Chemistry, Analytical
Siti Masturah Binti Fakhruddin, Kosuke Ino, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku
Summary: Bipolar electrode-based electrochromic devices have gained attention for analytical applications such as health monitoring, diagnostics, and chemical sensing. The review summarizes available electrochromic materials, discusses different device types, and reviews biological and chemical analytical demonstrations. The future developments of BPE-based electrochromic devices in analytical applications are then highlighted.
Article
Electrochemistry
Kosuke Ino, Keika Komatsu, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku
Summary: This study presents a method for electrochemiluminescence (ECL) imaging to visualize cellular adhesion on hydrogel scaffolds. By culturing human umbilical vein endothelial cells on a Matrigel-coated indium tin oxide (ITO) electrode and using an ECL solution and specific voltage, ECL signals were generated, allowing for imaging of cellular adhesion.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yoshinobu Utagawa, Kosuke Ino, Tatsuki Kumagai, Kaoru Hiramoto, Masahiro Takinoue, Yuji Nashimoto, Hitoshi Shiku
Summary: A novel electrochemical strategy for gluing hydrogel fibers is presented in this study. The method involves using microelectrodes to generate chemicals for crosslinking the fibers. The results show that the electrochemical glue is non-toxic to cells, making it a promising method for tissue engineering and organ-on-a-chip development.
Article
Chemistry, Analytical
Kaoru Hiramoto, Kazuyuki Iwase, Yoshinobu Utagawa, Yuji Nashimoto, Itaru Honma, Kosuke Ino, Hitoshi Shiku
Summary: Endothelial cells are commonly used for vascular biology studies. Recent advancements in tissue engineering have enabled the development of three-dimensional culture systems for vascular endothelial cells, which can serve as physiologically relevant models. To facilitate research, an electrochemical device has been developed for detecting nitric oxide (NO), a crucial molecule in the vasculature, to evaluate endothelial cells cultured in a 3D environment.
ANALYTICAL SCIENCES
(2022)
Article
Biophysics
Yuji Nashimoto, Rei Mukomoto, Takuto Imaizumi, Takato Terai, Shotaro Shishido, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Kunishige Onuma, Masahiro Inoue, Hitoshi Shiku
Summary: Microphysiological systems (MPSs) with three-dimensional (3D) cultured models have the potential to mimic human health and disease conditions. This study designed a microfluidic device with sensor capabilities to analyze oxygen metabolism in vascularized 3D tissue models. The platform was able to integrate a perfusable vascular network with 3D cultured cells and monitor changes in oxygen metabolism in a quantitative, non-invasive, and real-time manner.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Kazuya Fujimoto, Scott Erickson, Masamune Nakayama, Hiroki Ihara, Kei Sugihara, Yuji Nashimoto, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa
Summary: Blood vessel morphology is influenced by mechanical and biochemical cues, such as flow-induced shear stress and pericytes. This study reveals that angiogenesis occurs at locations with relatively low shear stresses (0.5-1.5 dyn cm(-2)) and that pericytes regulate vascular diameter. Furthermore, inhibiting platelet-derived growth factor receptor beta decreases pericyte coverage without affecting vessel diameter.
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku
Summary: In this study, we developed an electrochemical endotoxin sensor using an interdigitated array electrode (IDAE) device. The IDAE device showed high redox cycling efficiency and signal amplification rate. The sensor successfully detected p-aminophenol (pAP) released from Boc-Leu-Gly-Arg-p-aminophenol (LGR-pAP) as a product of an endotoxin-induced LAL reaction. The sensor could detect as low as 0.7 and 1.0 endotoxin unit/L after the LAL reaction for 1 h and 45 min, respectively, and these values were within the cut-off range for ultrapure dialysis fluid. Therefore, the highly sensitive endotoxin sensor is useful for ensuring medical safety.
Article
Biochemistry & Molecular Biology
Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Hitoshi Shiku
Summary: Hydrogels are widely used in cell culture applications, and specific structures are necessary for fabricating tissues and organs. Existing techniques for producing multiple-branched hydrogels are inefficient and time-consuming. To address this issue, a simple and rapid fabrication method based on viscous fingering is proposed, which only requires two plates. High-viscosity solution is introduced into the space between the plates and one plate is peeled off, resulting in the formation of multi-branched structures. This approach allows for the successful fabrication of multi-branched hydrogels within 1 minute.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Instruments & Instrumentation
Takeshi Hori, Yuya Ito, Bibek Raut, Serge Ostrovidov, Yuji Nashimoto, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji
Summary: With the increasing global aging population, the number of patients with retinal diseases requiring long-term treatment is expected to rise. Researchers have developed a 3D-printed refillable drug delivery system (DDS) that fits the curvature of the eyeball, providing a less invasive and continuous drug administration method. In laboratory tests, the DDS demonstrated sustained release of a model drug over 200 days and was able to undergo ten successive drug refills without any issues. In vivo experiments in rabbit sclera also showed promising results, indicating the potential of this DDS for long-term treatment of retinal diseases.
SENSORS AND MATERIALS
(2023)
Review
Chemistry, Physical
An Konno, Kosuke Ino, Yoshinobu Utagawa, Hitoshi Shiku
Summary: Microphysiological systems (MPSs) are cell culture systems that use mammalian cells to construct organs and tissues in vitro. Electrochemical sensors have become popular in MPSs due to their simplicity and sensitivity. This review summarizes recent advances in electrochemical imaging of MPSs and provides insightful perspectives.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Engineering, Biomedical
Yoshinobu Utagawa, Kosuke Ino, Masahiro Takinoue, Hitoshi Shiku
Summary: This study presents a simple and rapid method for fabricating core-shell hydrogel fibers for cell culture applications. The core fibers are prepared using interfacial polyelectrolyte complexation with chitosan and DNA, and then coated with calcium alginate shell hydrogels. This method has wide applications in tissue engineering and cell analysis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Analytical
Chisato Ito, Kosuke Ino, Kaoru Hiramoto, Yuji Nashimoto, Hitoshi Shiku
Summary: When a glass capillary filled with a low ion concentration solution has a nanopore at its tip, the ion concentration at the tip is higher than expected due to the formation of an electrical double layer inside the pore wall. This phenomenon, known as ion current rectification (ICR), has potential applications in bioassay methodology and biosensing procedures. However, ICR can only be induced under low ion concentrations, limiting its use under physiological conditions. This study investigates the effects of ion concentration on ICR using phosphate buffer solutions (PBS) and demonstrates successful monitoring of ICR even under physiological conditions, opening up possibilities for ICR-based biosensing applications.
Article
Chemistry, Analytical
Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Kazuyuki Iwase, Yuji Nashimoto, Itaru Honma, Hitoshi Shiku
Summary: In this study, a novel microfluidic device with a porous membrane electrode was developed for the in situ monitoring of nitric oxide (NO) released by endothelial cells in real time. This device successfully measured NO released by human umbilical vein endothelial cells and recorded the changes in NO release with culture time. This research contributes to the development of organ-on-a-chip technology for real-time in situ cell analyses.
ANALYTICAL CHEMISTRY
(2023)