Article
Medicine, Research & Experimental
Tingting Lv, Yu Zhang, XingZhao Ji, Shengnan Sun, Li Xu, Weixia Ma, Yi Liu, Qiang Wan
Summary: GCN5L1 regulates the acetylation and intracellular trafficking of TFAM, playing an important role in AKI-associated mitochondrial impairment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Cell Biology
Irshad Ahmad, Annie E. Newell-Fugate
Summary: This review focuses on the effects of androgens on mitochondrial structure and function, including biogenesis, fusion/fission, mitophagy, bioenergetics, and reactive oxygen species production. Although there have been numerous studies on the effects of androgens in these areas of mitochondrial function, the precise mechanisms by which androgens cause these effects remain unclear. Future research should focus on the direct effects of androgens on mitochondrial function.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jerzy Kaleczyc, Ewa Lepiarczyk
Summary: This review paper explores the influence of androgens on pelvic autonomic pathways in male mammals, highlighting significant differences between rats and pigs in terms of the effects of testosterone deprivation on pelvic neurons. The study also discusses the clinicopathological consequences of testosterone deprivation for the functioning of urogenital organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Urology & Nephrology
Mark C. Markowski, Hao Wang, Rana Sullivan, Irina Rifkind, Victoria Sinibaldi, Michael T. Schweizer, Benjamin A. Teply, Nduku Ngomba, Wei Fu, Michael A. Carducci, Channing J. Paller, Catherine H. Marshall, Mario A. Eisenberger, Jun Luo, Emmanuel S. Antonarakis, Samuel R. Denmeade
Summary: The study shows that cyclic high-dose testosterone injections have clinical activity in mCRPC patients, but responses may differ based on prior AR-targeted therapy. Rechallenge with AR-targeted therapy following BAT did not improve outcomes in AR-V7 positive patients.
Review
Oncology
Adriana Aguilar, Jacques Planas, Enrique Trilla, Juan Morote
Summary: This systematic review analyzes the current evidence for assessing the efficacy of medical castration using biochemical markers. The available evidence suggests that the current method of measuring serum testosterone levels during medical castration is of low quality. Immunoassays were predominantly used instead of the recommended liquid chromatography with tandem mass spectrometry (LC-MSMS) method to measure low testosterone concentrations. Longitudinal studies measuring serum testosterone with LC-MSMS, serum-free testosterone, and luteinising hormone are needed to determine their clinical impact.
Article
Biochemistry & Molecular Biology
Sunao Shimizu, Shuya Kasai, Hiromi Yamazaki, Yota Tatara, Junsei Mimura, Mate Janos Engler, Kunikazu Tanji, Yoshikazu Nikaido, Takuro Inoue, Hiroyuki Suganuma, Koichi Wakabayashi, Ken Itoh
Summary: Sulforaphane (SFN) has shown to improve age-related cognitive decline. This study found that broccoli sprout powder containing sulforaphane glucosinolate (SGS) can prevent cognitive decline in senescence-accelerated mice by maintaining mitochondrial function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ramesh Singh, Huan Meng, Tao Shen, Lance Edward V. Lumahan, Steven Nguyen, Hong Shen, Subhamoy Dasgupta, Li Qin, Dileep Karri, Bokai Zhu, Feng Yang, Cristian Coarfa, Bert W. O'Malley, Ping Yi
Summary: Castration-resistant prostate cancer (CRPC) is a major clinical challenge, and the androgen receptor (AR) is a critical oncogenic player. This study identifies TRAF4 as a key mediator of AR-regulated transcriptional reprogramming in CRPC, promoting its development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Maria Santa Rocca, Giovanni Minervini, Cinzia Vinanzi, Alberto Bottacin, Federica Lia, Carlo Foresta, Maria Pennuto, Alberto Ferlin
Summary: This study evaluated the frequency and type of androgen receptor (AR) gene variants in a large cohort of infertile males. The study found that patients with AR gene variants had lower sperm count, higher testosterone concentration, and higher androgen sensitivity index compared to patients without variants. Two novel potentially pathogenic AR variants were identified. Based on these findings, AR sequencing is suggested as a routine genetic test in cases of idiopathic oligozoospermia with high testosterone levels.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Cell Biology
Ping Ren, Fangchao Gong, Liang Chang, Xiaodong Hong, Lei Xing, Hong Zhang
Summary: The study demonstrated that zafirlukast enhances mitochondrial function by promoting mitochondrial respiration and biogenesis in human bronchial epithelial cells. This effect is mediated through upregulation of PGC-1 alpha expression and activation of the CREB pathway.
JOURNAL OF MOLECULAR HISTOLOGY
(2021)
Article
Medicine, Research & Experimental
Sue Jin Moon, Byong Chang Jeong, Hwa Jin Kim, Joung Eun Lim, Hye-Jeong Kim, Ghee Young Kwon, Joshua A. Jackman, Jeong Hoon Kim
Summary: The study identified BCT as a potent inhibitor targeting both AR-FL and AR-V7 activities in CRPC, effectively suppressing tumor growth and metastasis. Mechanistically, BCT disrupts the interaction of HSP90 with AR-FL/AR-V7, leading to their degradation and showing promising therapeutic potential against CRPC.
Review
Biochemistry & Molecular Biology
Carlos Cardanho-Ramos, Vanessa Alexandra Morais
Summary: Neurons primarily rely on mitochondria for ATP production and Ca2+ homeostasis, with different pools of mitochondria in each sub-compartment. Mitochondria are generated in the cell body and travel to the synapse, but in long cells like motor neurons, biogenesis also occurs at the periphery. Mitochondrial biogenesis in the cell body is triggered by Ca2+ and ATP levels, while mechanisms promoting biogenesis away from the cell body are less understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Almudena Zapatero, Ana Alvarez, Araceli Guerrero, Xavier Maldonado, Carmen Gonzalez San Segundo, Maria A. Cabeza, Carmen Martin de Vidales, Josep M. Sole, Agusti Pedro Olive, Francesc Casas, Ana Boladeras, Maria L. Vazquez de la Torre, Susana Vara, Felipe A. Calvo
Summary: The study found that additional serum testosterone suppression below 20 ng/dL did not necessarily lead to better outcomes compared to levels between 20-49 ng/dL in patients with localized prostate cancer. Time to testosterone recovery after androgen deprivation therapy and high-dose radiotherapy did not impact clinical failure.
RADIOTHERAPY AND ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ying Jiang, Da Li, Zhiqiang Du, Jie Li, Rongrong Lu, Qin Zhou, Qi Wang, Haohao Zhu
Summary: The study investigated the protective effects of Perampanel on mitochondrial biogenesis and bioenergetics in human neuronal cells. Results showed that Perampanel stimulated mitochondrial biogenesis and improved mitochondrial bioenergetics.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Engineering, Environmental
Yi Zhao, Xue-Nan Li, Hao Zhang, Jia-Gen Cui, Jia-Xin Wang, Ming-Shan Chen, Jin-Long Li
Summary: Male infertility is a significant concern due to the decline in sperm quality worldwide. Phthalates, particularly DEHP or its metabolite MEHP, have negative effects on male reproductive development and function, leading to reduced fertility. Lycopene, a natural antioxidant, has the potential to be used as a therapeutic option for male infertility. This research demonstrates that DEHP reduces testosterone production in Leydig cells and impairs secretory function in Sertoli cells, resulting in impaired spermatogenesis. Additionally, MEHP causes mitochondrial and oxidative damage, posing a serious threat to spermatogenesis. However, LYC supplementation reverses these changes. Mechanistically, DEHP disrupts the T/AR signaling pathway, contributing to male infertility. Overall, this study highlights the critical role of the T/AR signal transduction in male fertility and provides promising insights into the protective role of LYC in phthalate-induced male reproductive disorders.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Gastroenterology & Hepatology
Di Zhang, Jingjing Fan, Hui Liu, Guobin Qiu, Sheng Cui
Summary: The study reveals that testosterone enhances taurine synthesis by interacting with androgen receptor and binding to cysteine sulfinate decarboxylase (CSAD) promoter zone. Estrogen has no significant effects on liver CSAD expression or taurine synthesis in male mice, indicating gender differences in the effects of sex steroids on taurine synthesis in the liver.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Lin-zhen Zhang, Li He, Ning Wang, Jia-hua An, Gen Zhang, Jin Chai, Yu-jie Wu, Chang-jiu Dai, Xiao-han Li, Ting Lian, Ming-zhou Li, Long Jin
Summary: This study identified a natural antisense transcript, APMAP-AS, which is associated with lipid synthesis and positively regulates adipogenic differentiation. APMAP-AS forms an RNA-RNA duplex with APMAP, increasing its stability and promoting lipid metabolism while inhibiting the expression of inflammatory factors.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Genetics & Heredity
Zhiming Wang, Jin Chai, Yuhao Wang, Yiren Gu, Keren Long, Mingzhou Li, Long Jin
Summary: Animal fat deposition has a significant impact on meat flavor and texture. In this study, we investigated the regulatory role of lncPLAAT3-AS in promoting preadipocyte proliferation and adipocyte differentiation. Our findings suggest that lncPLAAT3-AS upregulates PLAAT3 expression through a sponge-like mechanism involving miR-503-5p binding.
Article
Agriculture, Dairy & Animal Science
Hulong Lei, Qian Du, Naisheng Lu, Xueyuan Jiang, Mingzhou Li, Dong Xia, Keren Long
Summary: This study compared the effects of copper sulfate and copper glycinate on fecal microbiota and metabolites in pigs, and found that copper glycinate had more positive effects on gut health.
Article
Biochemistry & Molecular Biology
Xiaokai Li, Zhining Zhong, Ruowei Zhang, Jiaman Zhang, Yu Zhang, Sha Zeng, Qinjiao Du, Haoming Wang, Songling Zhang, Lu Lu, Mingzhou Li, Keren Long
Summary: In this study, a muscular dystrophy mouse model was created by Ptrf knockout, and single-nucleus RNA sequencing was used to investigate the transcriptional changes in skeletal muscle. The study revealed significant alterations in the transcriptome of different types of muscle fibers and mononuclear cells in response to muscular dystrophy, providing valuable insights into the molecular mechanisms of muscular dystrophy.
Article
Multidisciplinary Sciences
Long Jin, Danyang Wang, Jiaman Zhang, Pengliang Liu, Yujie Wang, Yu Lin, Can Liu, Ziyin Han, Keren Long, Diyan Li, Yu Jiang, Guisen Li, Yu Zhang, Jingyi Bai, Xiaokai Li, Jing Li, Lu Lu, Fanli Kong, Xun Wang, Hua Li, Zhiqing Huang, Jideng Ma, Xiaolan Fan, Linyuan Shen, Li Zhu, Yanzhi Jiang, Guoqing Tang, Bin Feng, Bo Zeng, Liangpeng Ge, Xuewei Li, Qianzi Tang, Zhihua Zhang, Mingzhou Li
Summary: Using a pig model, the study investigates how chromatin architecture in adipose tissue is regulated in relation to obesity. Analysis of the data reveals similarities and differences in chromatin architecture between humans and pigs, providing valuable insights into the regulation of obesity-related genes.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Yujie Wang, Xun Wang, Yi Luo, Jiaman Zhang, Yu Lin, Jie Wu, Bo Zeng, Lei Liu, Peiqi Yan, Jiyuan Liang, Hongrui Guo, Long Jin, Qianzi Tang, Keren Long, Mingzhou Li
Summary: This study generated a de novo pigeon genome assembly and conducted a high resolution spatio-temporal transcriptomic analysis of the crop epithelium in pigeons. The study identified "lactation"-related genes involved in lipid and protein metabolism and revealed extensive reorganization of promoter-enhancer interactions linked to the expression of these genes. The spatial localization of gene expression in specific epithelial layers was correlated with phenotypic changes in the crop.
Article
Biology
Qin Zou, Rong Yuan, Yu Zhang, Yifei Wang, Ting Zheng, Rui Shi, Mei Zhang, Yujing Li, Kaixin Fei, Ran Feng, Binyun Pan, Xinyue Zhang, Zhengyin Gong, Li Zhu, Guoqing Tang, Mingzhou Li, Xuewei Li, Yanzhi Jiang
Summary: Studying cellular specialization in different anatomical regions of pig skin can enhance our understanding of skin information and provide insights for human skin research.
Article
Geriatrics & Gerontology
Linyuan Shen, Tianci Liao, Qiuyang Chen, Yuhang Lei, Linghui Wang, Hao Gu, Yanhao Qiu, Ting Zheng, Yiting Yang, Chenggang Wei, Lei Chen, Ye Zhao, Lili Niu, Shunhua Zhang, Yan Zhu, Mingzhou Li, Jinyong Wang, Xuewei Li, Mailin Gan, Li Zhu
Summary: This study explores the role of tRNA-derived small RNAs (tsRNAs), specifically 5'tiRNA-Gly, in skeletal muscle regeneration. It is found that 5'tiRNA-Gly is significantly up-regulated after muscle injury and promotes inflammation through the TGF-beta signaling pathway. These findings provide a new potential target for skeletal muscle regeneration treatment.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Physiology
Jinwei Zhang, Yang Shen, Guitao Yang, Jing Sun, Chuang Tang, Hao Liang, Jideng Ma, Xiaoqian Wu, Haoran Cao, Meng Wu, Yuchun Ding, Mingzhou Li, Zuohua Liu, Liangpeng Ge
Summary: This study analyzed the impact of commensal microbiota on the host's mucosal immunity. It found that the absence of commensal microbiota led to smaller Peyer's patches in the gastrointestinal tract, particularly regarding B and T cells. Gene expression analysis revealed that commensal microbiota plays an important role in the development of mucosal immunity in the intestine. Overall, this study revealed the regulatory mechanisms of commensal microbiota in the host's Peyer's patches and emphasized the importance of early microbial colonization for mucosal immunity development.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Weiqiang Liu, Pingfen Zhu, Meng Li, Zihao Li, Yang Yu, Gaoming Liu, Juan Du, Xiao Wang, Jing Yang, Ran Tian, Inge Seim, Alaattin Kaya, Mingzhou Li, Ming Li, Vadim N. Gladyshev, Xuming Zhou
Summary: The variation in lifespan among mammals is significant, and the study aims to uncover the evolutionary forces and molecular features related to longevity. Comparative transcriptomics analysis of liver, kidney, and brain tissues of 103 mammalian species revealed that only a few genes have common expression patterns with longevity. However, pathways related to translation fidelity and methionine restriction showed correlation with lifespan. The study suggests that lifespan regulation through gene expression is driven by polygenic and indirect natural selection.
Article
Biochemistry & Molecular Biology
Jingyi Bai, Yu Lin, Jiaman Zhang, Ziyu Chen, Yujie Wang, Mingzhou Li, Jing Li
Summary: The study used ATAC-seq and rRNA-depleted RNA-seq to analyze the chromatin accessibility and transcriptional features in multiple tissues of newborn pigs and during porcine liver development. The researchers obtained a comprehensive annotation of consensus ATAC-seq peaks for each tissue and developmental stage, and found that tissue-specific accessible promoters were associated with active transcription and tissue-specific functions. Additionally, they observed a close association between dynamic chromatin accessibility and hepatic function transition during liver postnatal development. This study expands our understanding of epigenetic regulation in mammalian tissues and organ development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jing Yang, Xingxing Zhu, Rui Wang, Mingzhou Li, Qianzi Tang
Summary: In this study, we comprehensively evaluated the performance of 24 popular state-of-the-art methods for complete end-to-end Hi-C data analysis using manually curated or experimentally validated benchmark datasets. Our results indicate that HiC-Pro, DomainCaller, and Fit-Hi-C2 showed relatively balanced performances in different aspects, providing a reference for researchers to choose suitable Hi-C analysis tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Huan Liu, Dengke Pan, Pu Li, Dandan Wang, Bo Xia, Ruixin Zhang, Junfeng Lu, Xiangyang Xing, Jiaxiang Du, Xiao Zhang, Long Jin, Lin Jiang, Linong Yao, Mingzhou Li, Jiangwei Wu
Summary: A novel sepsis pig model is created by modifying the surgical strategy of cecal ligation and puncture (CLP), which for the first time recapitulates the whole course of sepsis in humans. Increased levels of ZBED6 in skeletal muscle are shown in this model and sepsis patients, and protection against sepsis-induced muscle wasting is demonstrated in ZBED6-deficient pigs. The direct target of ZBED6 is revealed to be DOCK3, and septic ZBED6-deficient pigs show increased DOCK3 expression in skeletal muscle and myocytes, activating the RAC1/PI3K/AKT pathway and protecting against sepsis-induced muscle wasting.
Article
Multidisciplinary Sciences
Pengliang Liu, Diyan Li, Jiaman Zhang, Mengnan He, Yan Li, Rui Liu, Mingzhou Li
Summary: The storage of lipids as energy in adipose tissue is conserved, but there are substantial differences in the physiological activities of adipose tissues among species. This study characterized the transcriptional and lipidomic profiles of subcutaneous and visceral adipose tissues across 15 vertebrate species, spanning more than 300 million years of evolution. The data revealed greater variation in AT transcriptome and lipidome among species than within the same species, providing a resource for future studies on functional differences among adipose tissues in vertebrate species.
Article
Biochemistry & Molecular Biology
Jing Li, Teng Yuan, Anjing Zhang, Peidong Yang, Li He, Keren Long, Chuang Tang, Li Chen, Mingzhou Li, Lu Lu
Summary: Macrophages are essential for immunity and play important roles in tissue homeostasis and metabolism. There is currently no standardized method for isolating and differentiating porcine macrophages. This study identified distinct M1 and M2 macrophage subsets in pigs and compared their gene expression profiles. The results showed that porcine macrophages have similar gene signatures to human and mouse macrophages. The study also demonstrated the potential prognostic value of macrophage signatures in differentiating pathogen infections.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)