Article
Cell Biology
Binqing Tang, Yingen Wu, Yada Zhang, Yanqi Cheng, Yuqin Wu, Hong Fang
Summary: This study explores the molecular mechanism behind the therapeutic effects of scorpion and centipede on asthma. The results demonstrate that scorpion and centipede can ameliorate asthma by promoting M2 macrophage polarization and increasing M2 macrophage-derived exosomes. These exosomes carry miR-30b-5p, which inhibits airway epithelial cell pyroptosis and alleviates severe asthma. These findings may have potential clinical applications in the treatment of asthma.
Article
Immunology
Hong-xia Ye, Guang-neng Liao, Ya-jun Dong, Lan Li, Xue-mei Wang, Jin Shu, Qu Zheng, Yan Jia
Summary: The down-regulation of miR-146a-5p in decidual macrophages is associated with M1/M2 polarization disorder in URSA. miR-146a-5p inhibits M1 polarization, promotes M2 polarization, and generates an anti-inflammatory microenvironment in vitro. In a murine model of URSA, intravenous injection of miR-146a-5p reduces embryo resorption rate and promotes M2 polarization of decidual macrophages.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yi-Yung Hung, Chen-Kai Chou, Yi-Chien Yang, Hung-Chun Fu, El-Wui Loh, Hong-Yo Kang
Summary: This study found that in patients with major depressive disorder, the expression levels of miR-146a in exosomes were higher, and patients who achieved remission showed lower levels of various microRNAs before treatment and increased levels after treatment. Let-7e, miR-145, and miR-146a showed acceptable discrimination between the remission and non-remission groups.
Article
Neurosciences
Jun Sun, Zhi Liao, Zhangyu Li, Hao Li, Zhimin Wu, Chuan Chen, Hui Wang
Summary: This study focused on investigating the roles and mechanisms of exosomes derived from Schwann cells under different conditions in regulating macrophagic sub-phenotypes and peripheral nerve injury repair. The study found that exosomes from normal oxygen condition Schwann cells promoted M2 macrophagic polarization and facilitated axon elongation, while exosomes from post-injury oxygen-glucose-deprivation-condition Schwann cells promoted M1 polarization and inhibited axon elongation. The down-regulation of miR-146a-5p was closely related to the shift of exosomes to pro-inflammatory phenotype. The study concluded that miR-146a-5p played an important role in regulating macrophagic phenotype and had implications for axonal regeneration and functional recovery.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ting Pan, Yan Wu, Xu Zhang, Jingfan Wang, Xingxing Wang, Qinyuan Gu, Changlin Xu, Yuanyuan Fan, Xinsheng Li, Ping Xie, Qinghuai Liu, Zizhong Hu
Summary: This study provides the first evidence that exosomes derived from lens epithelial cells can inhibit abnormal ocular neovascularization and attenuate angiogenesis. In vitro experiments demonstrated that these exosomes inhibited the proliferation, migration, and tube formation of endothelial cells under high glucose conditions. Mechanistically, exosomal miR-146a-5p targeted the NRAS coding sequence in endothelial cells, leading to the inactivation of the AKT/ERK signaling pathway.
Article
Cell Biology
Jianglong Chen, Tong Chen, Jin Zhou, Xiuhao Zhao, Qingfeng Sheng, Zhibao Lv
Summary: The study revealed that miR-146a-5p can inhibit the expression of NLRP3 inflammasome and its downstream inflammatory factors in NEC patients' intestines, alleviating inflammation and intestinal injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xuefeng Yang, Shuang Cai, Yue Shu, Xun Deng, Yuanwei Zhang, Nian He, Lei Wan, Xu Chen, Yan Qu, Shouyang Yu
Summary: The study showed an increase in the proportion of M2 macrophages in gastric cancer tissues, where M2 macrophages promoted cell proliferation and tumorigenesis through exosomal miR-487a targeting TIA1. These findings may contribute to the development of novel diagnostic and therapeutic methods for gastric cancer.
Article
Biochemistry & Molecular Biology
Kou-Gi Shyu, Bao-Wei Wang, Wei-Jen Fang, Chun-Ming Pan, Chiu-Mei Lin
Summary: The expression of macrophage-derived exosomal MALAT1 is increased by high glucose levels, which in turn suppresses the expression of miR-150-5p and promotes resistin expression in macrophages. Additionally, macrophage-derived exosomal MALAT1 may serve as a potential therapeutic target for vascular disease in diabetes mellitus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Cui Xia, Kang Zhu, Yanni Zhang, Jingguo Chen, Chao Yu, Tianxi Gao, Guoxi Zheng
Summary: This study investigates the impact of exosome-derived miR-146a-3p on macrophage polarization in allergic rhinitis (AR). It is found that miR-146a-3p facilitates macrophage M2 polarization by targeting VAV3 through the PI3K/AKT/mTOR pathway, suggesting that miR-146a-3p and VAV3 could serve as potential targets for novel therapeutic strategies in AR management.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Jiahui Gu, Shengrui Yang, Xueying Wang, Yining Wu, Jia Wei, Jian Xu
Summary: This study aimed to investigate the effects of hypoxic lung adenocarcinoma (LUAD)-derived exosomes on macrophage polarization and explore the underlying molecular mechanism. It was found that hypoxic LUAD cells released exosomes enriched with miR-1290, which could be transferred to macrophages. Overexpression of miR-1290 induced macrophage polarization into an M2 phenotype. Exosomal miR-1290 targeted the downstream gene SOCS3 to activate the STAT3 signaling pathway, promoting M2 macrophage polarization. Targeting exosomal miR-1290 may provide a potential immunotherapeutic strategy for LUAD.
Article
Biotechnology & Applied Microbiology
Zhu Wang, Wansen Sun, Ruiping Li, Yan Liu
Summary: The study reveals that exosomes secreted by M2 macrophages attenuate LPS-induced podocyte apoptosis by regulating the miR-93-5p/TLR4 axis, providing a new perspective for the treatment of patients with diabetic nephropathy.
Article
Biology
Ke Zhang, Yu-Jie Li, Lin-Jia Peng, Hui-Feng Gao, Lu-Ming Liu, Hao Chen
Summary: This study explores the role of M2 macrophage-derived exosomes in pancreatic cancer (PC). It is found that exosomal miR-193b-3p derived from M2 macrophages enhances the proliferation, migration, invasion, and glutamine uptake of PC cells by targeting TRIM62, resulting in the decrease of c-Myc ubiquitination.
Article
Immunology
Qi Zhang, Jiaqi Ban, Shuai Chang, Huiyan Qu, Jie Chen, Fangwei Liu
Summary: A novel exosomal circRNA, circRNA11:120406118|12040782, was identified in the peripheral serum of silicosis patients. It was found to regulate silica-stimulated macrophage pyroptosis through the circRNA11:120406118|12040782/miR30b-5p/NLRP3 network and could promote the activation of fibroblasts. Overexpressing miR-30b-5p, a crucial component of the regulatory network, could inhibit pyroptosis and attenuate silica-induced lung inflammation and fibrosis in mice. These findings provide valuable insights into the early diagnosis and treatment of silicosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Rachel E. Crossland, Jean Norden, Sakhila Ghimire, Mateja Kralj Juric, Kim F. Pearce, Clare Lendrem, Matthew Collin, Eva Mischak-Weissinger, Ernst Holler, Hildegard T. Greinix, Anne M. Dickinson
Summary: The study found that miR-155 and miR-146a were upregulated in gastrointestinal and skin tissue samples of aGvHD patients, and their expression levels in serum and urine samples were also associated with the risk of developing aGvHD. These results support the potential of miR-155 and miR-146a as biomarkers for aGvHD, but further investigation is needed to understand their role in generalized inflammation and specific pathophysiology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Longhui Yuan, Jingchao Yang, Fei Liu, Lan Li, Jingping Liu, Younan Chen, Jingqiu Cheng, Yanrong Lu, Yujia Yuan
Summary: Macrophage infiltration is a common characteristic of acute kidney injury (AKI). Exosomes-mediated cell communication between tubular epithelial cells (TECs) and macrophages (M phi) have been suggested to be involved in AKI. In this study, it was found that M phi-derived exosomes from AKI mice caused mitochondrial damage and induced TECs injury. The upregulation of miR-195a-5p in M phi(AKI) was identified, and it was revealed that miR-195a-5p may be produced in infiltrated M phi and shuttled into TECs via exosomes. Inhibition of miR-195a-5p alleviated the mitochondrial dysfunction and cell injury induced by exosomes from AKI mice.