Article
Food Science & Technology
Monika Okulicz, Iwona Hertig, Tomasz Szkudelski
Summary: Allyl isothiocyanate (AITC), a compound found in Brassica family plants, has shown diverse effects including anti-cancer, cardioprotective, neuroprotective, and anti-obesity abilities. However, in a diabetic rat model induced by a high fat diet and streptozotocin, AITC caused adverse effects such as decreased thyroid hormones, lipid disorders, and pancreatic hypertrophy. Although AITC lowered blood glucose levels and improved insulin sensitivity, its adverse effects on lipids and hormones may limit its potential as an anti-diabetic compound.
Article
Entomology
Peng Wang, Daniel G. Vassao, Bettina Raguschke, Michael J. Furlong, Myron P. Zalucki
Summary: The study investigated the effects of allyl glucosinolate and its product on Helicoverpa armigera development, finding that AITC had a greater negative impact on the insect's life-history traits. However, low concentrations of AITC seemed to have a positive effect on certain traits. Both toxins induced detoxification activity in the gut, which was related to diet protein concentration.
Article
Microbiology
Leandro Solmi, Hernan G. Rosli, Marina A. Pombo, Santiago Stalder, Franco R. Rossi, Fernando M. Romero, Oscar A. Ruiz, Andres Garriz
Summary: To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This study investigates how polyamine homeostasis is regulated in the plant environment by examining transcriptomic data of Pseudomonas syringae, an important phytopathogen. The analysis reveals that polyamine synthesis, gene expression activation, and energy production are induced in the early stages of disease, while synthesis is inhibited and transport is upregulated in later stages, correlating with the induction of virulence genes and nitrogen/carboxylic acid metabolism. It is also found that activation of plant defense mechanisms impacts bacterial polyamine synthesis, potentially reducing bacterial fitness in the plant environment. Furthermore, the data suggests that oxidative conditions require a decrease in polyamine production. The implications of these findings are discussed.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Emilio Stefani, Aleksa Obradovic, Katarina Gasic, Irem Altin, Ildiko K. Nagy, Tanis Kovacs
Summary: Xanthomonads, economically important plant pathogenic bacteria, can be effectively controlled by bacteriophage-based biopesticides which offer environmentally friendly solutions. Despite the advantages, limited products are available on the market due to the biological properties of bacteriophages and the complex registration process.
Article
Chemistry, Applied
Lars Andernach, Katja Witzel, Franziska S. Hanschen
Summary: Glucosinolates are precursors of isothiocyanates (ITCs). Amines were found to be enzymatic hydrolysis products of glucosinolates in Brassica vegetables. An endogenous ITC hydrolase (ITCase) in the plants is responsible for the conversion of ITCs to amines. The presence of amines from glucosinolates in the human diet was confirmed.
Article
Food Science & Technology
Enping Zheng, Zhihang Zheng, Shiya Ren, Huabin Zhou, Hailong Yang
Summary: This study found that treatment with AITC, especially at a concentration of 10 mu L/L, can significantly improve the ROS metabolism and quality of postharvest Coprinus comatus, slowing the accumulation of MDA and ROS and maintaining membrane integrity. However, high concentrations of AITC can accelerate ROS generation and increase electrolyte leakage rate.
FOOD QUALITY AND SAFETY
(2022)
Article
Food Science & Technology
Enping Zheng, Zhihang Zheng, Shiya Ren, Huabin Zhou, Hailong Yang
Summary: This study evaluated the effect of allyl isothiocyanate (AITC) on the quality and reactive oxygen species (ROS) metabolism of postharvest Coprinus comatus. The results showed that appropriate concentrations of AITC can delay the increase in xanthine oxidase activity, improve the activity of other ROS-related enzymes, and maintain membrane integrity. However, high concentrations of AITC can accelerate ROS generation and electrolyte leakage. Additionally, all AITC treatments suppressed the respiration rate and browning of C. comatus.
FOOD QUALITY AND SAFETY
(2022)
Article
Cell Biology
Ruzhou Zhao, Xingcheng Zhao, Xiaobo Wang, Yanqi Liu, Jie Yang, Shuai Jiang, Xiang Zhou, Bo Jiao, Lin Zhang, Yong Liu, Zhibin Yu
Summary: Fasting for 72 hours prior to extreme hypoxia exposure can reduce tissue injuries, maintain cardiac function, and improve survival rates in rats. Fasting reduces blood glucose levels and inhibits tissue mTOR activity, leading to decreased ATP consumption and increased reserves as well as improved mitochondrial oxygen utilization efficiency under acute hypoxia. Targeted regulation of mTOR could be a new strategy to enhance acute hypoxic tolerance in the body.
CELL DEATH & DISEASE
(2021)
Article
Chemistry, Organic
Trevan Klug, Adam Cronin, Eli O'Brien, Ryan Schioldager, Hunter Johnson, Cameron Gleason, Connor Schmid, Nathan Soderberg, Aashrita Manjunath, Duminda Liyanage, Horacio Lazaro, Joshua J. Kimball, Scott Eagon
Summary: A method for the synthesis of 2-aminooxazoles at 150 degrees C using microwave irradiation was developed, providing products with various functional groups in a short reaction time and moderate to good yields, without the need for recrystallization or flash chromatography.
TETRAHEDRON LETTERS
(2022)
Review
Plant Sciences
Sheo Shankar Pandey
Summary: Iron is a crucial element for the growth and survival of all forms of life, serving as a catalyst in various enzymatic reactions. However, excessive iron accumulation can be toxic due to the production of reactive oxygen species. To maintain iron homeostasis, organisms have developed intricate molecular regulatory networks.
Article
Food Science & Technology
Yohei Hareyama, Mitsunori Tarao, Koki Toyota, Tomohiro Furukawa, Yoshiharu Fujii, Masayo Kushiro
Summary: Aflatoxins produced by Aspergillus fungi can contaminate food and pose a serious threat to human health and the economy. This study explored the potential of isothiocyanates (ITCs) as chemical agents to control aflatoxigenic fungi. The results showed that benzyl ITC (BITC) was the strongest inhibitor of Aspergillus flavus growth and aflatoxin B-1 production in a dissolved state, while allyl ITC (AITC) inhibited A. flavus growth effectively in a gaseous state.
Article
Biochemistry & Molecular Biology
Xinyu Zhou, Xinhui Huang, Chunting Wu, Yongjie Ma, Weiqi Li, Jinqiu Hu, Rong Li, Fuli Ya
Summary: Sulforaphane (SFN), an isothiocyanate found in cruciferous vegetables, has been shown to modulate platelet mitochondrial dysfunction and hyperactivity by up-regulating the cAMP/PKA pathway through decreasing PDE3A activity. This study provides evidence for the cardioprotective effects of SFN in attenuating platelet dysfunction and hyperreactivity.
Article
Multidisciplinary Sciences
Luisa M. Castano, Andres F. Gomez, Jesus Gil, Diego Durango
Summary: Perinaphthenone exhibited significant antifungal activity against three economically important fungi of the citrus, with an enhanced inhibitory effect against Fusarium spp. under direct light exposure. Metabolism studies revealed that the transformation of perinaphthenone by the fungi led to the formation of two major products, which showed slightly lower fungistatic activities compared to perinaphthenone, indicating a detoxification process. These findings suggest that perinaphthenone could serve as a potential antifungal agent or structural template for the development of new fungicide compounds.
Article
Environmental Sciences
Golam Jalal Ahammed, Youxin Yang
Summary: Plants detoxify toxic metals by accumulating diverse metabolites, with some metabolites chelating metal ions. In addition to classical detoxification pathways involving thiol-containing compounds, secondary metabolites like anthocyanins have emerged as playing a significant role in metal detoxification. Anthocyanins, with their ability to scavenge reactive oxygen species, contribute to enhanced arsenic tolerance in plants and may offer potential for improving crop yield and food safety in arsenic polluted areas.
ENVIRONMENTAL POLLUTION
(2022)
Review
Microbiology
Julie Baltenneck, Sylvie Reverchon, Florence Hommais
Summary: Quorum sensing is a mechanism by which bacterial populations coordinate gene expression through chemical communication, commonly used by pathogens to control virulence factor and environmental adaptation gene expression. Several quorum sensing mechanisms have been identified in phytopathogenic bacteria, utilizing different signal molecules. Research focuses on major phytopathogenic bacteria and their quorum sensing systems.
Article
Biotechnology & Applied Microbiology
Heleen T. Ouboter, Arslan Arshad, Stefanie Berger, Jesus Gerardo Saucedo Sanchez, Huub J. M. op den Camp, Mike S. M. Jetten, Cornelia U. Welte, Julia M. Kurth
Summary: This study describes the biochemical properties of ACS and ACD enzymes from ANME-2 archaea, adding to our knowledge of these enzymes. It also validates the in situ activity of ACS in ANME-2d, demonstrating the conversion of acetate into methane.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Carolina Suarez, Thomas Hackl, Britt-Marie Wilen, Frank Persson, Per Hagelia, Mike S. M. Jetten, Paula Dalcin Martins
Summary: This study investigated the concrete biodeterioration in the Oslofjord subsea road tunnel and found bacterial genomes related to nitrogen and metal cycling in biofilm samples, which might contribute to the deterioration of concrete.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Ecology
Xiaomin Wang, Shanyun Wang, Yuanhe Yang, Hanqin Tian, Mike S. M. Jetten, Changchun Song, Guibing Zhu
Summary: Since the start of the Anthropocene, northern seasonally frozen peatlands have been warming rapidly, resulting in increased nitrogen mineralization and substantial emissions of nitrous oxide (N2O) during the thawing periods in spring. Heterotrophic bacterial and fungal denitrification were identified as the main sources of N2O in frozen peatland profiles. Thawing significantly stimulates the expression of genes encoding N2O-producing protein complexes, leading to high N2O emissions during the spring thawing period.
Article
Engineering, Environmental
Ping Han, Ana B. Rios-Miguel, Xiufeng Tang, Yaochun Yu, Li-Jun Zhou, Lijun Hou, Min Liu, Dongyao Sun, Mike S. M. Jetten, Cornelia U. Welte, Yujie Men, Sebastian Lucker
Summary: This study investigated the metabolic capacity of comammox Nitrospira inopinata and Nitrospira kreftii to transform benzimidazole fungicides. The comammox bacteria were found to actively transform all the tested fungicides, except for thiabendazole. The identified transformation products suggested hydroxylation, S-oxidation, and glycosylation as the major biotransformation pathways.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Multidisciplinary Sciences
Rob A. Schmitz, Stijn H. Peeters, Sepehr S. Mohammadi, Tom Berben, Timo van Erven, Carmen A. Iosif, Theo van Alen, Wouter Versantvoort, Mike S. M. Jetten, Huub J. M. op den Camp, Arjan Pol
Summary: Aerobic methanotrophic bacteria can survive in sulfide-rich environments, despite the inhibitory effects of hydrogen sulfide (H2S) on methane oxidation and aerobic respiration. This is due to the upregulation of a sulfide-insensitive terminal oxidase, allowing the microorganism to oxidize both methane and H2S simultaneously.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Carmen Hogendoorn, Arjan Pol, Rob de Graaf, Paul B. White, Rob Mesman, Peter M. van Galen, Theo A. van Alen, Geert Cremers, Robert S. Jansen, Mike S. M. Jetten, Huub J. M. Op den Camp
Summary: The production of organic molecules can be achieved by synthesizing them from CO2 and a cheap energy source, instead of relying on fossil fuels. Volcanic and geothermal areas, which have abundant CO2 and reduced inorganic gases, provide habitats where novel chemolithoautotrophic microorganisms for organic compound synthesis can be discovered. A thermoacidophilic, autotrophic H-2-oxidizing microorganism called Candidatus Hydrogenisulfobacillus filiaventi R50 was found to fix CO2 and produce more than 0.54 mol of organic carbon per mole of fixed CO2. This discovery opens up possibilities for the sustainable production of important biomolecules using chemolithoautotrophic microorganisms.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Physiology
Wouter Mes, Philippe Kersten, Roel M. Maas, Ep H. Eding, Mike S. M. Jetten, Henk Siepel, Sebastian Lucker, Marnix Gorissen, Maartje A. H. J. Van Kessel
Summary: Accumulation of ammonia is a challenge in intensive aquaculture, and feeding strategy and dietary protein content can impact fish growth and nitrogen metabolism. Nitrogen cycle bacteria in the gills convert ammonia to N-2 and potentially explain the unaccounted nitrogen in the balance.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Selmihan Sahin, Olivier N. Lemaire, Melissa Belhamri, Julia M. Kurth, Cornelia U. Welte, Tristan Wagner, Ross D. Milton
Summary: This article presents the electroenzymatic catalysis of a CO2-reducing-and-fixing biomachinery isolated from a thermophilic methanogen. The enzyme demonstrates high Faradaic efficiency and formate accumulation, making it a promising tool for the conversion of greenhouse gases into stable formate for various biotechnological purposes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Environmental Sciences
Ana B. Rios-Miguel, Tamara J. H. M. van Bergen, Caterina Zillien, Ad M. J. Ragas, Rosalie van Zelm, Mike S. M. Jetten, A. Jan Hendriks, Cornelia U. Welte
Summary: Organic micropollutants (OMPs), including pharmaceuticals and pesticides, can persist in water at low concentrations and pose a threat to aquatic ecosystems and drinking water quality. The effectiveness of wastewater treatment plants (WWTPs) in removing OMPs varies due to factors such as low concentrations, stable chemical structures, and suboptimal conditions. This review discusses these factors, focusing on the adaptation of microorganisms to degrade OMPs. Recommendations are made to improve prediction models and optimize microbial treatment strategies for OMP removal.
Article
Microbiology
Jessica Venetz, Olga M. Zygadlowska, Wytze K. Lenstra, Niels A. G. M. van Helmond, Guylaine H. L. Nuijten, Anna J. Wallenius, Paula Dalcin Martins, Caroline P. Slomp, Mike S. M. Jetten, Annelies J. Veraart
Summary: This study explores the potential and drivers of microbial methane removal in a stratified coastal marine system. It reveals the presence of diverse methanotrophic communities with high genomic versatility and adaptability, which contribute to the functional resilience and efficiency of methane removal.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Geochemistry & Geophysics
Rachel Schwartz-Narbonne, Philippe Schaeffer, Sabine K. Lengger, Jerome Blewett, D. Martin Jones, Estelle Motsch, Andrew Crombie, Mike S. -M. Jetten, Deirdre Mikkelsen, Philippe Normand, Guylaine H. L. Nuijten, Richard D. Pancost, Darci Rush
Summary: Lipid biomarkers, such as BHT isomers, can be used to trace bacterially mediated nitrogen and carbon cycle processes affecting greenhouse gas emissions. The source of BHT-34R in freshwater environments can be determined by its 813C value, and the presence of BHT-34R with low 813C values can be used as a novel tool to trace anammox in freshwater environments.
ORGANIC GEOCHEMISTRY
(2023)
Article
Environmental Sciences
Wouter Mes, Sebastian Lucker, Mike S. M. Jetten, Henk Siepel, Marnix Gorissen, Maartje A. H. J. van Kessel
Summary: Recirculating aquaculture systems (RAS) are used to grow fish, with biofilters containing nitrogen-cycling microorganisms. This study compared the microbial communities of RAS water and biofilter with the gut and gill microbiomes of zebrafish and common carp. The location of sampling had a stronger effect on community composition than the fish species, with distinct differences observed between the fish-associated microbiomes and the RAS microbiomes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Annabel Legierse, Quinten Struik, Garrett Smith, Maider J. Echeveste Medrano, Stefan Weideveld, Gijs van Dijk, Alfons J. P. Smolders, Mike Jetten, Annelies J. Veraart, Cornelia U. Welte, Martyna Glodowska
Summary: Agricultural drainage ditches with high nitrate input contribute to eutrophication and greenhouse gas emissions. Nitrate-dependent anaerobic methane oxidation (N-DAMO) could be an effective strategy to simultaneously remove methane (CH4) and nitrate (NO3-). Microcosm experiments were conducted using sediment and water samples from different ditches, and the results showed that N-DAMO effectively reduced CH4 and NO3- concentrations in brackish peatland ditch sediment. However, in freshwater sediments, N-DAMO was outcompeted by heterotrophic denitrifiers, resulting in negligible effects on CH4 and NO3- removal. Overall, our study suggests that N-DAMO bioaugmentation may be a potential approach to decrease NO3- concentrations and CH4 emission in brackish ecosystems where native microbial communities are not efficient in denitrification.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Microbiology
Martyna Glodowska, Yinxiao Ma, Garrett Smith, Andreas Kappler, Mike Jetten, Cornelia U. Welte
Summary: The input of nitrate to the aquifer inhibits Fe(III) reduction and As mobilization preventing As groundwater contamination. However, the addition of N-DAMO enrichment cultures led to Fe(III) reduction and stimulated As and Mn release into the water. Our results suggest that input of N fertilizers to the aquifer decreases As mobility and that CH4 cannot serve as an electron donor for NO3- reduction.
FEMS MICROBIOLOGY ECOLOGY
(2023)
Article
Microbiology
Koen A. J. Pelsma, Daniel A. M. Verhagen, Joshua F. Dean, Mike S. M. Jetten, Cornelia U. Welte
Summary: Global urbanization has influenced microbial communities in waterways over the past millennium. Canal wall biofilms in Dutch cities have varying methane oxidation potentials, with some cities showing high potentials. Salinity was identified as the main driver of biofilm community composition. The oxidation potential of the biofilms was not correlated with methane emissions, but rather with the presence or absence of methane-oxidizing bacteria.
FEMS MICROBIOLOGY ECOLOGY
(2023)