Article
Microbiology
Siu Lung Ng, Sophia Kammann, Gabi Steinbach, Tobias Hoffmann, Peter J. Yunker, Brian K. Hammer
Summary: Organisms sense external cues and use transcription factors to regulate gene expression and adapt to new niches. This study identifies the regulatory element of the major type VI locus in Vibrio cholerae and discovers a single nucleotide polymorphism that controls the killing activity of the type VI secretion system. This finding highlights the role of noncoding DNA in pathogen adaptation.
Article
Immunology
Jennifer Y. Cho, Rui Liu, Ansel Hsiao
Summary: The commensal microbes of the gut microbiota play an important role in defending against gastrointestinal pathogens. Different commensal communities contribute to individual differences in susceptibility to infections. This study reveals the different response mechanisms of susceptible and infection-resistant microbial communities to Vibrio cholerae infection, including differential gene expression related to oxidative stress and biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jant Cres Caigoy, Toshi Shimamoto, Asish Kumar Mukhopadhyay, Sumio Shinoda, Tadashi Shimamoto
Summary: We investigated the impact of hapR sequence mutations on biofilm formation in Vibrio cholerae. Our results demonstrate that HapR mutations strongly influence biofilm formation, with sequence polymorphisms disrupting DNA-binding sites or dimerization of HapR resulting in more robust biofilms in V. cholerae.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Microbiology
Jennifer K. Teschler, Carey D. Nadell, Knut Drescher, Fitnat H. Yildiz
Summary: Biofilms are a growth mode in which microbial communities are structured and embedded in a polymeric extracellular matrix. In this study, the biofilm formation of Vibrio cholerae is focused and the current understanding of its formation, including attachment, matrix components, dynamics, regulation, and dispersal, is summarized. The decision to form or disperse from biofilms is regulated by a complex network that integrates various environmental inputs.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Guangli Wang, Chan Fan, Hui Wang, Chengyi Jia, Xiaoting Li, Jianru Yang, Tao Zhang, Song Gao, Xun Min, Jian Huang
Summary: This study reveals a novel regulatory role of the FHA domain protein TagH in controlling the hemolytic activity of Vibrio cholerae, in addition to regulating the T6SS. TagH negatively regulates HlyA expression at the transcriptional and post-translational levels, and the phosphopeptide binding sites of the FHA domain play a crucial role in the regulation of hemolytic activity. Deletion of tagH enhances the intestinal pathogenicity and extraintestinal invasion ability of V. cholerae, which mostly rely on the expression of HlyA.
Article
Microbiology
Joao P. Pombo, Stephan P. Ebenberger, Anna M. Mueller, Heimo Wolinski, Stefan Schild
Summary: Vibrio cholerae represses 192 genes during biofilm formation, which are involved in metabolism, regulation, surface association, transmembrane transport, motility, and chemotaxis. These genes are not essential for biofilm formation and their presence can interfere with the ordered development of biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Ryan A. Nuttall, Pia H. Moisander
Summary: This study compared biofilm formation of V. cyclitrophicus strains isolated from copepods and seawater, and found previously unknown biofilm mechanisms in the S-strains. Calcium enhanced biofilms in the L-strains, and the GFP-L-strain attached to live copepods in higher numbers, suggesting the presence of distinct mechanisms supporting their colonization of copepods.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Erin C. Young, Jackson T. Baumgartner, Ece Karatan, Misty L. Kuhn
Summary: Biofilm formation in Vibrio cholerae is partly regulated by norspermidine, spermidine, and spermine through a signaling pathway involving the periplasmic protein NspS and the integral membrane c-di-GMP phosphodiesterase MbaA. In this pathway, polyamine binding to NspS likely affects its interaction with MbaA, leading to either activation or inhibition of biofilm formation. Mutagenesis studies identified important amino acids in NspS for its function, shedding light on the novel signaling pathway and the role of periplasmic binding proteins in V. cholerae.
Article
Multidisciplinary Sciences
Benjamin Kostiuk, Francis J. Santoriello, Laura Diaz-Satizabal, Fabiana Bisaro, Kyung-Jo Lee, Anna N. Dhody, Daniele Provenzano, Daniel Unterweger, Stefan Pukatzki
Summary: It was found that classical Vibrio cholerae underwent sequential mutations in T6SS genetic determinants, making them disadvantaged in competition with the 7th pandemic El Tor biotype strains.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Jennifer K. Teschler, Eva Jimenez-Siebert, Hannah Jeckel, Praveen K. Singh, Jin Hwan Park, Stefan Pukatzki, Carey D. Nadell, Knut Drescher, Fitnat H. Yildiz
Summary: This study demonstrates that the Vibrio cholerae type six secretion system (T6SS) can actively kill prey strains within the interior of biofilm populations with substantial impact on population dynamics. We additionally show that the response regulator VxrB contributes to both T6SS killing and protection from T6SS killing within biofilms.
Article
Multidisciplinary Sciences
Minh Nguyen, Tzu-Hui Wu, Katie J. Danielson, Nabeel M. Khan, John Zhijia Zhang, Lisa Craig
Summary: Many bacteria have dynamic filaments called Type IV pili (T4P) that play various roles in colonization and dissemination. A specific segment in the TcpF protein functions as an export signal (ES) recognized by the Vibrio cholerae toxin-coregulated pilus (TCP). This ES is proteolyzed from TcpF during secretion and is capable of mediating the export of Neisseria gonorrhoeae FbpA by V. cholerae. Specificity is achieved through the interaction between the ES and TcpB, a minor pilin protein. These findings provide insights into the mechanism of TcpF delivery across the outer membrane.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Alexis Proutiere, Natalia C. Drebes Dorr, Loriane Bader, Sandrine Stutzmann, Lisa C. Metzger, Sandrine Isaac, Nicolas Chiaruttini, Melanie Blokesch
Summary: Vibrio cholerae, the pathogen responsible for cholera, can overcome the colonization barrier created by the host's natural microbiota through its type VI secretion system (T6SS). Recent research suggests that T6SS activity is low in pandemic V. cholerae strains, but our study found modest T6SS activity in most tested strains. We also observed T6SS activity through immunodetection of the T6SS protein Hcp in culture supernatants. Additionally, we found that T6SS production is sporadic within the bacterial population and is dependent on the VxrAB two-component system.
Article
Biochemistry & Molecular Biology
Manuel Maestre-Reyna, Wei-Cheng Huang, Wen-Jin Wu, Praveen K. Singh, Raimo Hartmann, Po-Hsun Wang, Cheng-Chung Lee, Takaaki Hikima, Masaki Yamamoto, Yoshitaka Bessho, Knut Drescher, Ming-Daw Tsai, Andrew H-J Wang
Summary: Vibrio cholerae switches from biofilm-bound state to planktonic state through an autocatalytic proteolysis process involving the major biofilm scaffolding protein RbmA, which is triggered by phosphate-dependent induced proximity activation mechanism. Mutants of RbmA that are defective in autoproteolysis lead to larger and mechanically stronger biofilms, indicating a new sensory role of this biofilm scaffolding element affecting microbial community homeostasis and rheological properties.
Article
Biology
Kelsey Barrasso, Denise Chac, Meti D. Debela, Catherine Geigel, Anjali Steenhaut, Abigail Rivera Seda, Chelsea N. Dunmire, Jason B. Harris, Regina C. Larocque, Firas S. Midani, Firdausi Qadri, Jing Yan, Ana A. Weil, Wai-Leung Ng, Melanie Blokesch
Summary: Recent studies suggest that the presence of Paracoccus aminovorans, a commensal bacterium, in the human intestine may enhance the colonization of Vibrio cholerae. The interaction between the two species was shown to form a dual-species biofilm structure with novel features, and this interaction was found to be dependent on the Vibrio exopolysaccharide and other major components of mature V. cholerae biofilm. These findings demonstrate that multispecies biofilm formation could be a mechanism used by gut microbes to increase the virulence of pathogens and potentially impact the outcomes of enteric infections.
Article
Microbiology
Matthew Jemielita, Ameya A. Mashruwala, Julie S. Valastyan, Ned S. Wingreen, Bonnie L. Bassler
Summary: Bacteria utilize quorum sensing to orchestrate collective behaviors, with Vibrio cholerae forming multicellular communities. This aggregation process consists of two subprograms - void formation and motility-dependent entry. The timing of aggregation is controlled by four extracellular proteases, ensuring reliable community formation.
Article
Microbiology
Loni Townsley, Fitnat H. Yildiz
ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Zachary C. Ruhe, Loni Townsley, Adam B. Wallace, Andrew King, Marjan W. Van der Woude, David A. Low, Fitnat H. Yildiz, Christopher S. Hayes
MOLECULAR MICROBIOLOGY
(2015)
Article
Microbiology
Andrew Rogers, Loni Townsley, Ana L. Gallego-Hernandez, Sinem Beyhan, Laura Kwuan, Fitnat H. Yildiz
JOURNAL OF BACTERIOLOGY
(2016)
Article
Multidisciplinary Sciences
Laura M. Sanchez, Andrew T. Cheng, Christopher J. A. Warner, Loni Townsley, Kelly C. Peach, Gabriel Navarro, Nicholas J. Shikuma, Walter M. Bray, Romina M. Riener, Fitnat H. Yildiz, Roger G. Linington
Review
Biochemistry & Molecular Biology
Loni Townsley, Elizabeth A. Shank
TRENDS IN MICROBIOLOGY
(2017)
Article
Microbiology
Loni Townsley, Sarah M. Yannarell, Tuanh Ngoc Huynh, Joshua J. Woodward, Elizabeth A. Shank
Article
Microbiology
Giordan Kitts, Krista M. Giglio, David Zamorano-Sanchez, Jin Hwan Park, Loni Townsley, Richard B. Cooley, Benjamin R. Wucher, Karl E. Klose, Carey D. Nadell, Fitnat H. Yildiz, Holger Sondermann
Article
Microbiology
Loni Townsley, Lews Caro, Hemant Kelkar, Elizabeth A. Shank
GENOME ANNOUNCEMENTS
(2016)