Article
Microbiology
Melissa J. Karau, Suzannah M. Schmidt-Malan, Scott A. Cunningham, Jayawant N. Mandrekar, Bobbi S. Pritt, Tiffany R. Keepers, Alisa W. Serio, Surya Chitra, Robin Patel
Summary: Omadacycline, vancomycin, and rifampin, as well as rifampin combination therapies, were evaluated in an experimental rat model of MRSA osteomyelitis. The results showed that rifampin combination therapy was effective in reducing MRSA recovery without developing resistance. The combination of omadacycline and rifampin yielded negative MRSA detection, suggesting its potential as a treatment for osteomyelitis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Monika Kumaraswamy, Kamilla Wiull, Bishnu Joshi, George Sakoulas, Armin Kousha, Gustav Vaaje-Kolstad, Mona Johannessen, Kristin Hegstad, Victor Nizet, Fatemeh Askarian
Summary: This study revealed that MRSA-derived MVs are capable of reducing the pathogen's susceptibility to VAN, thereby increasing its resistance to treatment, particularly in interactions with human neutrophils. Additionally, the elevated expression of proteins associated with antibiotic resistance in MVs may further impact MRSA's resistance.
Article
Infectious Diseases
Cheng-En Tsai, Chia-Jui Yang, Yu-Chung Chuang, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, Shan-Chwen Chang
Summary: This study aimed to determine the synergistic effects of combining CPT with DAP, VAN, or LNZ against different MRSA strains. The results showed that the CPT-DAP combination exhibited significant synergistic bactericidal effects, while the CPT-VAN and CPT-LNZ combinations did not show synergistic activity. Therefore, DAP may be a better choice for combination therapy with CPT in the treatment of MRSA infections.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Jin Liang, Yuanfang Hu, Mingxia Fu, Na Li, Fengxia Wang, Xiaojun Yu, Bing Ji
Summary: The study investigated the molecular epidemiology of Staphylococcus aureus (S. aureus) in patients from the Yellow River Delta region and examined antibiotic resistance profiles in different genetic backgrounds. Antibiotic susceptibility testing, spa typing, and agr typing were conducted on all 204 strains. The results identified 39 MRSA strains and 22 hVISA isolates. The majority of MRSA isolates belonged to agrI and were associated with type IV SCCmec. The most common genotypes among MRSA population were ST59-t437-agrI-IVa and ST72-t2431-agrI-IVF. The study highlights the genetic diversity and drug resistance patterns among MSSA strains exhibiting phenotypic hVISA.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Infectious Diseases
Martyna Kasela, Mateusz Ossowski, Ewelina Dzikon, Katarzyna Ignatiuk, Lukasz Wlazlo, Anna Malm
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of infectious diseases in humans and animals, resulting in economic losses in human healthcare and the farming sector. The changing epidemiology of MRSA globally affects animal welfare and public health concerns. Livestock have high rates of MRSA colonization, raising questions about reservoirs and transmission, while companion animals vary in MRSA colonization and infection rates, impacting human health. This article highlights the circulation of animal-associated MRSA (AA-MRSA) in the environment and factors influencing its transmission, as well as successful strategies to limit its spread among animals and humans.
Article
Biotechnology & Applied Microbiology
Rong Huang, Guang-Qing Cai, Jian Li, Xi-Sheng Li, Hai-Ting Liu, Xue-Ling Shang, Jian-Dang Zhou, Xin-Min Nie, Rong Gui
Summary: A novel nano-drug delivery system, PLT@Ag-MOF-Vanc, was constructed for the treatment of MRSA infections, showing good antibacterial activity, biocompatibility, and targeting capabilities. It killed MRSA through multiple approaches and demonstrated superior anti-infective effects in a mouse MRSA pneumonia model compared to free vancomycin.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fateme Eskandari, Hossein Mofidi, Bahar Asheghi, Fateme Mohammadi, Ahmad Gholami
Summary: Nowadays, there is a growing concern about the inadequacy of new antimicrobials and the rise of antimicrobial resistance. In this study, the antimicrobial properties of zinc oxide nanoparticles (ZnONPs) were improved by coupling them with imidazolium (IM@ZnONPs). The results showed that IM@ZnONPs exhibited stronger antibacterial activities, especially against antimicrobial-resistant pathogens, compared to ZnONPs and zinc ions.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Pathology
Jill Parkes-smith, Haakon Bergh, Patrick N. A. Harris
Summary: Staphylococcus aureus bacteraemia has high morbidity and mortality, and timely use of effective antibiotics is crucial. Molecular diagnostics can shorten the time from blood culture positivity to identification of MRSA or MSSA.
Article
Immunology
Yifei Lu, Yingran Wang, Jing Wang, Yan Zhao, Qiu Zhong, Gang Li, Zhifeng Fu, Shuguang Lu
Summary: Endolysin LysP108 demonstrates promising antibacterial potential as it can lyse bacterial cell walls, inhibit bacterial biofilms, and work synergistically with vancomycin to reduce abscess size in MRSA-infected mice. The results indicate strong bactericidal effects against MRSA and the ability to significantly reduce subcutaneous abscess size when used in combination with vancomycin.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Nur Najihah Izzati Mat Rani, Xiang Yi Chen, Zahraa M. Al-Zubaidi, Hanisah Azhari, Tzar Mohd Nizam Khaitir, Pei Yuen Ng, Fhataheya Buang, Geok Chin Tan, Yin Ping Wong, Mazlina Mohd Said, Adeel Masood Butt, Azmy A. Hamid, Mohd Cairul Iqbal Mohd Amin
Summary: This study focused on the encapsulation of vancomycin into liposomes coated with a red blood cell membrane with a targeting ligand, and successfully achieved targeted drug delivery to bacterial cells. The conjugation of the targeting ligand was confirmed through various analyses, and the formulation exhibited controlled drug release. In vitro and in vivo experiments demonstrated the high antibacterial efficacy of the formulation against MRSA, with low toxicity to mammalian cells and human erythrocytes.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Immunology
Marin L. Schweizer, Kelly Richardson, Mary S. Vaughan Sarrazin, Michihiko Goto, Daniel J. Livorsi, Rajeshwari Nair, Bruce Alexander, Brice F. Beck, Michael P. Jones, Mireia Puig-Asensio, Daniel Suh, Madeline Ohl, Eli N. Perencevich
Summary: Among patients with methicillin-resistant Staphylococcus aureus bloodstream infections, switching to daptomycin is associated with lower 30-day mortality, especially when done within 3 days of initial vancomycin receipt. However, switching to daptomycin at any time during the first hospitalization did not significantly affect 30-day mortality.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Pharmacology & Pharmacy
Mengli Jin, Shuyue Zhu, Yating Tang, Xiangri Kong, Xingye Wang, Yufen Li, Shuang Jiang, Lin Wei, Chunjie Hu, Bingmei Wang, Wu Song
Summary: Antimicrobial resistance (AMR) is a global health threat, and there is a need for new anti-infective agents. Ayanin, a flavonoid extracted from Callicarpa nudiflora, was found to be an inhibitor of MRSA ClpP, suggesting its potential therapeutic effect against MRSA infections.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Pooi Yin Chung, Pey Lin Narissa Loh, Hui-min Neoh, Ramliza Ramli
Summary: Staphylococcus aureus is a significant threat to human health and has developed resistance to conventional antimicrobials. The presence of biofilms complicates treatment for infections caused by multi-drug resistant S. aureus. Alpha-amyrin has been shown to reduce biomass and inhibit metabolic activity of S. aureus. This study demonstrates that alpha-amyrin can reduce biofilm biomass of MSSA, MRSA, and VISA isolates, suggesting its potential as a therapeutic agent for chronic S. aureus infections.
Article
Immunology
Bingjie Wang, Yanlei Xu, Huilin Zhao, Xinyi Wang, Lulin Rao, Yinjuan Guo, Xie Yi, Longhua Hu, Shuying Chen, Lizhong Han, Junying Zhou, Guoxiu Xiang, Long Hu, Liang Chen, Fangyou Yu
Summary: The aim of this study was to investigate the genomic epidemiology of MRSA in China and identify the predominant lineages and their associated genomic and phenotypic characteristics. The study found that CC59, CC5, and CC8 were the major lineages, with CC59 replacing CC8 as the predominant lineage over time. Different lineages of MRSA exhibited varying levels of antimicrobial resistance and carried different resistance determinants. Additionally, different lineages carried multiple virulence genes involved in various functions.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Biotechnology & Applied Microbiology
Jiaping Li, Yonglu Huang, Yanyan Hu, Qiaoling Sun, Jiachang Cai, Hongwei Zhou, Danxia Gu, Gongxiang Chen, Yang Wang, Rong Zhang
Summary: The study utilized MALDI-TOF MS for determining colistin resistance in E. coli, establishing optimal incubation time, breakpoint concentration, and RG cut-off values. The model showed high sensitivity and specificity in distinguishing between susceptible and resistant strains, providing an effective tool for detecting colistin-resistant bacteria.
MICROBIAL BIOTECHNOLOGY
(2022)
Letter
Infectious Diseases
Qiaoling Sun, Xuemei Yang, Yonglu Huang, Yi Li, Jianping Lin, Daihai He, Congcong Liu, Sheng Chen, Rong Zhang
JOURNAL OF INFECTION
(2022)
Letter
Microbiology
Siheng Wang, Xiaoyang Ju, Ning Dong, Ruichao Li, Yan Li, Rong Zhang, Yonglu Huang, Hongwei Zhou
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Bo Fu, Dandan Yin, Chengtao Sun, Yingbo Shen, Dejun Liu, Rina Bai, Rong Zhang, Jianzhong Shen, Fupin Hu, Yang Wang
Summary: Increasing infections caused by bla(NDM)-carrying Klebsiella pneumoniae (NDMKP) pose an urgent threat to children with weakened immunity and limited antibiotic use. The isolates exhibit high resistance to carbapenems but are fully sensitive to other antibiotics. Molecular analysis reveals both clonal and horizontal transmission of bla(NDM) among NDM-KP strains in children's hospitals, with key plasmids and sequence type diversity contributing to their spread. This study provides important insights for pediatric clinicians in treating infections caused by NDM-KP.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yiming Li, Xiaomin Shi, Yang Zuo, Tian Li, Lu Liu, Zhangqi Shen, Jianzhong Shen, Rong Zhang, Shaolin Wang
Summary: This study established a high-throughput sequencing method that can simultaneously detect ARGs, mobile genetic element genes, and metal resistance genes in metagenomes. Compared to traditional metagenomic shotgun sequencing, this method has higher coverage, sensitivity, and diversity, with lower cost and computation burden. The method was validated in various samples and can serve as an efficient tool for monitoring and evaluating the evolution of ARGs.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Ning Dong, Congcong Liu, Yanyan Hu, Jiayue Lu, Yu Zeng, Gongxiang Chen, Sheng Chen, Rong Zhang
Summary: This study reports the first occurrence of extensively drug-resistant Pseudomonas aeruginosa strain PA166-2 carrying bla(IMP-45), tet(X6), and tmexCD3-toprJ3 on a single plasmid in chickens. The strain, belonging to ST313, was resistant to last-line antibiotics and the plasmid belonged to the incompatibility group Inc(pRBL16). The emergence of plasmid-mediated tigecycline resistance genes and carbapenemase genes from chickens expanded the global transmission of vital resistance genes. Control measures should be implemented to prevent further dissemination of such strains.
MICROBIOLOGY SPECTRUM
(2022)
Article
Genetics & Heredity
Yingbo Shen, Rong Zhang, Dongyan Shao, Lu Yang, Jiayue Lu, Congcong Liu, Xueyang Wang, Junyao Jiang, Boxuan Wang, Congming Wu, Julian Parkhill, Yang Wang, Timothy R. Walsh, George F. Gao, Zhangqi Shen
Summary: The emergence of mcr-1, a colistin resistance gene, has gained global attention. While the prevalence of mcr-1-positive Escherichia coli (MCRPEC) strains in human carriage has decreased between 2016 and 2019, the genetic differences in these strains remain largely unknown. Through a comparative genomic study, we found three major differences in MCRPEC strains between the two time points. These differences include changes in plasmid types, emergence of additional antibiotic resistance genes, and an increased proportion of virulence genes, indicating a potential public health threat.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Microbiology
Jin Tao, Dejun Liu, Jincheng Xiong, Leina Dou, Weishuai Zhai, Rong Zhang, Yang Wang, Jianzhong Shen, Kai Wen
Summary: In this study, a cascade system was developed for the rapid on-site detection of extensively drug-resistant (XDR) genes in Enterobacteriaceae. The system utilizes polyethylene glycol (PEG) 200-enhanced recombinase polymerase amplification (RPA), a modified Chelex-100 lysis method, and a horseradish peroxidase (HRP)-catalyzed lateral flow immunoassay (LFIA) biosensor. The system demonstrated high sensitivity and specificity, allowing for the detection of XDR genes in Enterobacteriaceae in less than 1 hour without the need for large-scale instruments.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Danxia Gu, Zelin Yan, Chang Cai, Jiaping Li, Yanyan Zhang, Yuchen Wu, Jiaxing Yang, Yonglu Huang, Rong Zhang, Yongning Wu
Summary: In this study, the efficacy of three different methods for the rapid detection of carbapenemases (KPC, NDM, IMP, OXA-48, and VIM) was evaluated. The NG-test Carba 5, colloidal gold immunoassay (CGI) test, and Xpert Carba-R assay showed reliable performances in carbapenemase detection, with Xpert Carba-R being recommended for the detection of KPC variants.
Article
Microbiology
Yonglu Huang, Yuchen Wu, Chang Cai, Rong Zhang, Gongxiang Chen, Ning Dong
Summary: We identified a highly drug-resistant and virulent strain of Enterobacter hormaechei, ST133/C210017, using the Galleria mellonella infection model. Genomic analysis revealed the presence of antibiotic resistance genes bla(KPC-2) and mcr-9.1, as well as virulence-related genes iutAiucABCD and iroBCDEN. Comparative genomics with other ST133 E. hormaechei strains showed that they shared serotype O3 and a majority (77.5%) carried the IncHI2 superplasmids, which contribute to resistance, virulence, and host adaptation.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Weiyi Shen, Rong Zhang, Jiachang Cai
Summary: This study investigates the molecular characteristics and transferability of plasmid-borne linezolid resistance genes optrA, cfr, poxtA2, and cfr(D) in linezolid-resistant Enterococcus faecalis DM86 from retail meat. The presence of known linezolid resistance genes was detected using PCR analysis. Conjugation experiments were conducted to evaluate the transferability of these resistance genes. The complete genome of DM86 was obtained, and four linezolid resistance genes were identified on three plasmids.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Environmental Sciences
Xiaoyang Ju, Yuchen Wu, Gongxiang Chen, Rong Zhang
Summary: A carbapenem-resistant Escherichia coli strain C-SRM-3 was isolated from hospital wastewater effluent in Hangzhou city, China in March 2022. The isolate belonged to the high-risk clone ST410 and carried bla(NDM-13) gene responsible for carbapenem resistance in E. coli. The detection of bla(NDM-13) gene in hospital wastewater highlights the importance of continuous monitoring to prevent the spread of carbapenem-resistant bacteria.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Automation & Control Systems
Jiayue Lu, Jifan Chen, Ling Huang, Siheng Wang, Yingbo Shen, Sheng Chen, Zhangqi Shen, Rong Zhang
Summary: Antimicrobial resistance is a global public health problem, causing over 700,000 deaths annually. Researchers have developed a novel method that uses artificial intelligence to analyze Raman spectra and identify microbes and their susceptibility to antibiotics at genotype and phenotype levels. The method achieved high accuracy in bacterial species classification, identification of antimicrobial-resistance genes, and prediction of resistance phenotypes, outperforming other machine learning algorithms. The results suggest that Raman spectroscopy combined with LSTM analysis can be used for rapid bacterial species identification, detection of resistance genes, and assessment of drug-resistance phenotypes.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Infectious Diseases
Jingjing Du, Yiqing Tao, Jiaxing Yang, Jiachang Cai, Hongwei Zhou, Rong Zhang, Yanyan Hu
Summary: Brucellosis is a zoonotic disease caused by Brucella spp. with a high prevalence in northern cities of China. This case report presents a spinal infection caused by B. melitensis in a 67-year-old man from a non-endemic area of southern China. The patient's condition improved with treatment.
INFECTION AND DRUG RESISTANCE
(2023)
Letter
Infectious Diseases
Jiayue Lu, Yao Wang, Congcong Liu, Qiaoling Sun, Yu Zeng, Chang Cai, Zhangqi Shen, Rong Zhang
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)