期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 146, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/58469
关键词
Medicine; Issue 146; Brown adipose tissue; cold-activation; adult human; fluorodeoxyglucose; positron emission tomography; computed tomography; obesity; thermoregulation
资金
- Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases [Z01 DK071014, DK075116-02]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [ZICDK071013, ZIADK071014] Funding Source: NIH RePORTER
In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT's ability to expend energy has made it a potential target for novel therapies to ameliorate obesity and associated metabolic disorders in humans. Though this tissue has been well studied in small animals, BAT's thermogenic capacity in humans remains largely unknown due to the difficulties of measuring its volume, activity, and distribution. Identifying and quantifying active human BAT is commonly performed using F-18-Fluorodeoxyglucose (F-18-FDG) positron emission tomography and computed tomography (PET/CT) scans following cold-exposure or pharmacological activation. Here we describe a detailed image-analysis approach to quantify total-body human BAT from F-18-FDG PET/CT scans using an open-source software. We demonstrate the drawing of user-specified regions of interest to identify metabolically active adipose tissue while avoiding common non-BAT tissues, to measure BAT volume and activity, and to further characterize its anatomical distribution. Although this rigorous approach is time-consuming, we believe it will ultimately provide a foundation to develop future automated BAT quantification algorithms.
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