Review
Immunology
Pamella Constantino-Teles, Albane Jouault, Lhousseine Touqui, Alessandra Mattos Saliba
Summary: Pseudomonas aeruginosa, an opportunistic pathogen, causes respiratory infections by utilizing various virulence factors, such as bacterial lipids and enzymes, and by interacting with host lipids. These mechanisms play important roles in the establishment and persistence of P. aeruginosa in the lungs, as well as in modulating host immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Samantha L. Durfey, Sudhakar Pipavath, Anna Li, Anh T. Vo, Anina Ratjen, Suzanne Carter, Sarah J. Morgan, Matthew C. Radey, Brenda Grogan, Stephen J. Salipante, Michael J. Welsh, David A. Stoltz, Christopher H. Goss, Edward F. McKone, Pradeep K. Singh
Summary: Studies show that chronic CF infections may persist despite improvements induced by CFTR modulators and aggressive antibiotic treatment. Combining CFTR modulators with intensive antibiotics may be most effective for patients with higher CFTR activity. Challenges remain in improving the health of people with CF.
Review
Microbiology
Tegan M. Hibbert, Marvin Whiteley, Stephen A. Renshaw, Daniel R. Neill, Joanne L. Fothergill
Summary: Pseudomonas aeruginosa is an opportunistic pathogen responsible for respiratory infections in patients with chronic respiratory conditions. The development of anti-virulence therapeutics may provide new treatment options, but the challenge lies in replicating the virulence gene expression patterns in the complex pulmonary environment.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Margherita Degasperi, Riccardo Sgarra, Mario Mardirossian, Sabrina Pacor, Massimo Maschio, Marco Scocchi
Summary: This study developed a prodrug to extend the peptide chain of an antimicrobial peptide and added a sequence that can activate the peptide at the site of infection/inflammation, enabling a slow and targeted release. The prodrug showed good antimicrobial activity and lower cytotoxicity in the presence of elastase.
Article
Microbiology
Madeline Mei, Preston Pheng, Detriana Kurzeja-Edwards, Stephen P. Diggle
Summary: Chronic, highly antibiotic-resistant infections in cystic fibrosis lungs contribute to increasing morbidity and mortality. Pseudomonas aeruginosa, a common CF pathogen, exhibits resistance to multiple antibiotics, contributing to antimicrobial resistance. This study found that the diversity of P. aeruginosa variants within CF lung infections leads to varying susceptibility to R-pyocins, and suggests that LPS packing density may play a significant role in R-pyocin susceptibility among CF variants.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Corinne Bure, Caroline Le Senechal, Luis Macias, Caroline Tokarski, Sebastien Vilain, Jennifer S. Brodbelt
Summary: Lipopolysaccharides (LPS) form the outermost layer of Gram-negative bacteria, playing a crucial role in bacterial infections. Understanding the structure of lipid A, which anchors LPS to the bacterial outer membrane, is important in elucidating LPS virulence and toxicity. Various successful strategies utilizing tandem mass spectrometry have been employed for the structural analysis of lipid A, revealing new acyl chain positional isomers in the lipid A profile of Pseudomonas aeruginosa PAO1.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Kelly Kweng, Andrea Benedetti, Yvonne Yau, Valerie Waters, Dao Nguyen
Summary: This study found that PA isolates from new-onset CF infections exhibit strain-specific susceptibility to neutrophil antibacterial functions, and PA isolates resistant to neutrophil phagocytosis are independent risk factors for failed tobramycin eradication. Experimental results on neutrophil phagocytosis and intracellular killing showed that isolates from persistent infections were more resistant compared to those from eradicated infections.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Respiratory System
Luca Cavinato, Francesco R. Luly, Valentina Pastore, Daniele Chiappetta, Gloria Sangiorgi, Eva Ferrara, Pia Baiocchi, Giuseppe Mandarello, Giuseppe Cimino, Paola Del Porto, Fiorentina Ascenzioni
Summary: Cystic fibrosis (CF) is characterized by chronic bacterial lung infection and inflammation due to mutations in CF transmembrane conductance regulator (CFTR). Monocytes and monocyte-derived macrophages in CF have been shown to have defective phagocytosis and antimicrobial activity against relevant pathogens. This study evaluated the effect of CFTR triple modulator therapy on CF monocyte activity against Pseudomonas aeruginosa. The results showed that CF monocytes exhibited improved phagocytic and microbicidal activity, reduced oxidative burst, and decreased inflammatory response after ETI therapy.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Michele Iafisco, Francesca Carella, Lorenzo Degli Esposti, Alessio Adamiano, Daniele Catalucci, Jessica Modica, Alessandra Bragonzi, Alberto Vitali, Riccardo Torelli, Maurizio Sanguinetti, Francesca Bugli
Summary: The use of inhalable calcium phosphate nanoparticles functionalized with colistin shows promising potential for improving the efficacy of cystic fibrosis treatment by enhancing drug delivery to bacteria in the airways.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Katie A. Parsels, Keri A. Mastro, Jeffrey M. Steele, Stephen J. Thomas, Wesley D. Kufel
Summary: Cefiderocol is a novel siderophore cephalosporin that has shown activity against clinically relevant MDR Gram-negative bacteria. FDA-approved indications include complicated urinary tract infections and hospital-acquired bacterial pneumonia. Clinical trials have demonstrated non-inferiority to standard treatments, but higher all-cause mortality was observed in certain cases.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Adrian Bouzas Munoz, Juan Antonio Gimenez-Bastida, Aurora Garcia Tejedor, Claudia Monika Haros, Marta Gomez de Cedron, Ana Ramirez de Molina, Jose Moises Laparra Llopis
Summary: The study showed that administration of SETIs improved survival and decreased tumor burden by modulating monocyte-derived macrophages, playing a key role in diet-induced HCC development. This suggests a potential therapeutic strategy to improve the hepatic innate immune response and create a better antitumoral environment in early stages of HCC development.
Article
Microbiology
Verena Ducret, Melina Abdou, Catarina Goncalves Milho, Sara Leoni, Oriane Martin--Pelaud, Antoine Sandoz, Ines Segovia Campos, Mary-Lou Tercier-Waeber, Martina Valentini, Karl Perron
Summary: Zinc is essential for the survival, virulence, and antibiotic resistance of Pseudomonas aeruginosa. By studying the expression dynamics of the Zn homeostasis network in different environments, researchers discovered new components and regulatory links within the system. This work reveals a complex and adaptive network that plays a crucial role in pathogen spread and host colonization.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Sophie Camens, Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Peter-John Wormald, Sarah Vreugde
Summary: The isolated phage PA4 showed lytic ability against clinical isolates of P. aeruginosa, indicating its potential in clinical applications.
Article
Microbiology
Donghoon Kang, Natalia V. Kirienko
Summary: Pseudomonas aeruginosa is a multidrug-resistant pathogen that causes acute infections in immunocompromised patients, using the siderophore pyoverdine to facilitate infection and virulence. Inhibiting pyoverdine production can reduce pathogen virulence and may be a promising therapeutic target.
Article
Immunology
M. Codagnone, E. Cianci, A. Lamolinara, V. C. Mari, A. Nespoli, E. Isopi, D. Mattoscio, M. Arita, A. Bragonzi, M. Iezzi, M. Romano, A. Recchiuti
MUCOSAL IMMUNOLOGY
(2018)
Article
Microbiology
Domenico Mattoscio, Chiara Casadio, Claudia Miccolo, Fausto Maffini, Andrea Raimondi, Carlo Tacchetti, Tarik Gheit, Marta Tagliabue, Viviana E. Galimberti, Francesca De Lorenzi, Michael Pawlita, Fausto Chiesa, Mohssen Ansarin, Massimo Tommasino, Susanna Chiocca
Review
Biochemistry & Molecular Biology
Domenico Mattoscio, Alessandro Medda, Susanna Chiocca
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Review
Biochemistry & Molecular Biology
Antonio Recchiuti, Elisa Isopi, Mario Romano, Domenico Mattoscio
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Antonio Recchiuti, Sara Patruno, Domenico Mattoscio, Elisa Isopi, Antonella Pomilio, Alessia Lamolinara, Manuela Iezzi, Romina Pecce, Mario Romano
Summary: An excessive and unresolved inflammatory response is one of the reasons underlying severe COVID-19, and the investigation of endogenous pathways to resolve inflammation is crucial for mitigating inflammation caused by SARS-CoV-2 infection. Individuals with preexisting pathologies like cystic fibrosis exhibit some specificity in immune responses to SARS-CoV-2. Furthermore, resolvins D1 and D2 have beneficial regulatory effects in reducing SARS-CoV-2-induced inflammation.
Article
Oncology
Domenico Mattoscio, Elisa Isopi, Alessia Lamolinara, Sara Patruno, Alessandro Medda, Federica De Cecco, Susanna Chiocca, Manuela Iezzi, Mario Romano, Antonio Recchiuti
Summary: The study shows that RvD1 can reduce cancer growth by activating PMN anti-cancer activities and promoting the recruitment of anti-tumor monocytes. In HPV-positive tumor models, RvD1 can decrease the proliferation of cancer cells and lead to better clinical outcomes for patients.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Domenico Mattoscio, Giulia Ferri, Claudia Miccolo, Susanna Chiocca, Mario Romano, Antonio Recchiuti
Summary: In human medicine, predicting the progression of tumor development and resolution of inflammation remains challenging. By evaluating the expression level of genes involved in the synthesis, signaling, and metabolism of D-series resolvin, a cumulative score was used to analyze clinical, cellular, and molecular features of tumors. Higher scores were associated with better clinical outcomes and enhanced anti-tumor immunity in head and neck squamous cell carcinoma patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Giulia Ferri, Matteo Mucci, Domenico Mattoscio, Antonio Recchiuti
Summary: The COVID-19 pandemic has highlighted the importance of acute inflammation and its resolution for protecting humans from pathogenic viruses and minimizing immune-mediated damages. Specialized proresolving lipid mediators (SPM) derived from essential fatty acids play a crucial role in resolving inflammation and regulating adaptive immunity during viral infections. Exploration of SPM biosynthesis and bioactions in virus-mediated human diseases can lead to new therapeutic approaches for controlling infections, enhancing host immunity, and preventing organ damage.
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2023)
Meeting Abstract
Pediatrics
A. Recchiuti, D. Mattoscio, E. Isopi, A. Pomilio, A. Lamolinara, M. Iezzi, M. Romano
PEDIATRIC PULMONOLOGY
(2019)
Meeting Abstract
Pediatrics
D. Mattoscio, E. Isopi, A. Lamolinara, M. Iezzi, M. Romano, A. Recchiuti
PEDIATRIC PULMONOLOGY
(2019)
Meeting Abstract
Pediatrics
E. Isopi, D. Mattoscio, M. Romano, A. Recchiuti
PEDIATRIC PULMONOLOGY
(2019)
Meeting Abstract
Pediatrics
D. Mattoscio, E. Isopi, V. C. Mari, M. Codagnone, E. Cianci, A. Lamolinara, M. Iezzi, M. Romano, A. Recchiuti
PEDIATRIC PULMONOLOGY
(2018)
Meeting Abstract
Pediatrics
E. Isopi, D. Mattoscio, V. C. Mari, M. Codagnone, E. Cianci, A. Lamolinara, M. Iezzi, M. Romano, A. Recchiuti
PEDIATRIC PULMONOLOGY
(2018)
Article
Biology
Domenico Mattoscio, Andrea Raimondi, Carlo Tacchetti, Susanna Chiocca