Article
Cell Biology
Yilin Pang, Xinjie Liu, Xu Wang, Xuelian Shi, Lei Ma, Yan Zhang, Tiangang Zhou, Chenxi Zhao, Xu Zhang, Baoyou Fan, Jian Hao, Wenxiang Li, Xiaoqing Zhao, Rong Zhang, Songlin Zhou, Xiaohong Kong, Shiqing Feng, Xue Yao
Summary: Edaravone, an FDA-approved drug, has a neuroprotective effect on spinal cord injury and other central nervous system diseases. It exerts this effect by inhibiting ferroptosis and reducing neuroinflammation. The mechanism may be applicable to other neurological diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Biao Yang, Chengzhen Liang, Di Chen, Feng Cheng, Yuang Zhang, Shaoke Wang, Jiawei Shu, Xianpeng Huang, Jingkai Wang, Kaishun Xia, Liwei Ying, Kesi Shi, Chenggui Wang, Xuhua Wang, Fangcai Li, Qian Zhao, Qixin Chen
Summary: The current effective method for treating spinal cord injury (SCI) involves using a hydrogel called agarose/gelatin/polypyrrole (Aga/Gel/PPy, AGP3) to fill the injured cavity and promote neural stem cell (NSC) differentiation. This AGP3 hydrogel showed excellent biocompatibility and promoted neuronal differentiation while inhibiting astrocyte growth. In vivo studies demonstrated that the AGP3 hydrogel covered tissue defects, reduced the size of the injured area, and facilitated functional recovery by promoting endogenous neurogenesis. RNA sequencing analysis revealed that AGP3 hydrogel modulated the expression of neurogenesis-related genes through intracellular calcium signaling pathways.
BIOACTIVE MATERIALS
(2022)
Review
Cell Biology
Emily A. B. Gilbert, Nishanth Lakshman, Kylie S. K. Lau, Cindi M. Morshead
Summary: Spinal cord injury affects millions of people worldwide, and there is currently no cure. Utilizing endogenous neural stem cells (NSCs) to replace lost cells and promote structural repair is a promising approach. However, there are still many unanswered questions regarding the heterogeneity of NSCs, their interaction with the environment, and factors that enhance their response.
Article
Neurosciences
Lei Wang, Sujie Gu, Jinlu Gan, Yi Tian, Fangcheng Zhang, Hongyang Zhao, Deqiang Lei
Summary: Transplanting genetically modified neural stem cells (NSCs) overexpressing nerve growth factor (NGF) following spinal cord injury (SCI) can significantly enhance motor function and reduce tissue damage at the injury site in rats. The transplanted NGF-NSCs modulate the microenvironment, facilitate endogenous neurogenesis, and exhibit neuroprotective effects mediated by TrkA activation, upregulation of CREB, and microRNA-132.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Immunology
Emma Schmidt, Pamela Raposo, Romana Vavrek, Karim Fouad
Summary: Inflammation can enhance neuronal sprouting and recovery in rats following spinal cord injury, and systemic lipopolysaccharide treatment in the subacute stage can improve the recovery of the ipsilesional forelimb.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Nanoscience & Nanotechnology
Guangfei Wei, Dongdong Jiang, Shuai Hu, Zhiyuan Yang, Zifan Zhang, Wei Li, Weihua Cai, Dongfei Liu
Summary: Neuroinflammation following spinal cord injury can worsen damage; controlling cytokine release can protect and aid recovery of the injured spinal cord.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Neurosciences
Kuileung Tong, Shiming Li, Guoliang Chen, Chao Ma, Xizhe Liu, Shaoyu Liu, Ningning Chen
Summary: Endogenous neural stem cells (eNSCs) undergo necroptosis during spinal cord injury repair. In vitro experiments show that the necroptosis induced by TSZ treatment regulates the phenotype of NSCs. Necroptosis inhibitors improve NSC survival and phenotype maintenance in vitro and contribute to neuroprotection and repair in vivo. Blocking necroptosis of eNSCs may be a potential therapeutic strategy for treating spinal cord injury.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Hongfei Ke, Hongru Yang, Yijing Zhao, Tingting Li, Danqing Xin, Chengcheng Gai, Zige Jiang, Zhen Wang
Summary: This study introduces a method for constructing and testing 3D gelatin microsphere (GM) scaffolds based on convenient preparation of gelatin microspheres using microfluidic devices. These scaffolds demonstrate good biocompatibility, biodegradability, and porosity. They can effectively bridge injury gaps, establish nerve connections and signal transductions, mitigate inflammatory microenvironments, and reduce glial scar formation.
Article
Biotechnology & Applied Microbiology
Guang Kong, Wu Xiong, Cong Li, Chenyu Xiao, Siming Wang, Wenbo Li, Xiangjun Chen, Juan Wang, Sheng Chen, Yongjie Zhang, Jun Gu, Jin Fan, Zhengshuai Jin
Summary: This study explores the influence of Treg cells on the recovery of BSCB after spinal cord injury (SCI) and its underlying mechanism. The findings suggest that Treg cell-derived exosomes can affect BSCB integrity and motor function by regulating the expression of IRAK1. This provides novel insights for functional repair and inflammation limitation after SCI.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Weidong Wu, Shuaijun Jia, Hailiang Xu, Ziheng Gao, Zhiyuan Wang, Botao Lu, Yixiang Ai, Youjun Liu, Renfeng Liu, Tong Yang, Rongjin Luo, Chunping Hu, Lingbo Kong, Dageng Huang, Liang Yan, Zhimou Yang, Lei Zhu, Dingjun Hao
Summary: A strategy of using PDGF-MP hydrogel microspheres instead of bulk hydrogels can enhance the efficiency of PDGF-MP in activating its receptor, thus improving the therapeutic ability of neural stem cells (NSCs) in treating spinal cord injuries (SCI). In vitro experiments showed that PDGF-MP hydrogel microspheres maintained the proliferation and inhibited the apoptosis of NSCs in the presence of myelin extracts. In vivo experiments demonstrated that PDGF-MP hydrogel microspheres inhibited inflammatory cell infiltration and cell apoptosis, and promoted axonal regeneration, synapse formation, and angiogenesis in SCI rats.
Article
Materials Science, Biomaterials
Jun Zhou, Yaqi Wu, Zhijian Tang, Kaipeng Zou, Juan Chen, Zuowei Lei, Xueyan Wan, Yanchao Liu, Huaqiu Zhang, Yu Wang, Armin Blesch, Ting Lei, Shengwen Liu
Summary: Studies have shown that alginate capillary hydrogels can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can survive, proliferate, and differentiate into neurons within the capillaries in vitro. In animal experiments, a portion of the grafted cells can survive and differentiate into neurons, astrocytes, and oligodendrocytes. Furthermore, the grafted cells can promote the growth of host axons, form putative synapses with host neurons, improve electrophysiological conductivity, and partially restore locomotor function.
REGENERATIVE BIOMATERIALS
(2022)
Article
Chemistry, Physical
Yuxing Jiang, Hongtao Rong, Yifan Wang, Shange Liu, Peng Xu, Zhen Luo, Lamei Guo, Tao Zhu, Hongpan Rong, Dingsheng Wang, Jiatao Zhang, Yu Yi, Hao Wang
Summary: Oxidative stress and inflammation are key processes in traumatic spinal cord injury (SCI), and effective antioxidant therapy is challenging. Researchers developed a hollow Co-SAzyme, which effectively reduced reactive oxygen and nitrogen species (RONS) and inflammation in the secondary injury of SCI. The Co-SAzyme demonstrated versatile RONS elimination capabilities and facilitated sustained release of minocycline for neuroprotection. In vitro and in vivo experiments confirmed the Co-SAzyme's ability to reduce apoptosis and pro-inflammatory cytokines, and improve functional recovery and neural repairs in an SCI-rat model.
Article
Immunology
Yang Lu, Cheng Chen, Heng Wang, Rong Du, Jiawei Ji, Tao Xu, Changwei Yang, Xiaoqing Chen
Summary: After spinal cord injury, astrocyte-derived sEVs can reduce fibrosis formation and improve functional recovery, providing a new possibility for the study of SCI.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Neurosciences
Jiang-Hu Huang, Yong-Neng Chen, Hang He, Chun-Hui Fu, Zhao-Yi Xu, Fei-Yue Lin
Summary: This study demonstrates that Schwann cells-derived exosomes (SCs-Exos) can promote proliferation, migration, and tube formation of brain-derived endothelial cells (bEnd.3 cells). It is found that SCs-Exos express high levels of the pro-angiogenesis molecules, Integrin-beta 1. The angiogenic effect of SCs-Exos on bEnd.3 cells is dependent on Integrin-beta 1 expression. In an spinal cord injury (SCI) model, treatment with SCs-Exos attenuates tissue damage and enhances functional recovery by promoting angiogenesis, which requires the involvement of Integrin-beta 1.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Rui Liu, Ying Li, Ziyue Wang, Peng Chen, Yi Xie, Wensheng Qu, Minghuan Wang, Zhiyuan Yu, Xiang Luo
Summary: After spinal cord injury (SCI), immune cells and proinflammatory cytokines infiltrate the spinal cord and disrupt the microenvironment, hindering axon regeneration and functional recovery. Previous studies have shown that regulatory T cells (Tregs) can enter the central nervous system and suppress microglia during conditions like multiple sclerosis and stroke. However, the interaction between Tregs and microglia and their role in modulating the injured microenvironment after SCI remains unknown.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Engineering, Biomedical
Mo Li, Ying Wang, Jidi Zhang, Zheng Cao, Shuo Wang, Wei Zheng, Qian Li, Tianqi Zheng, Xiumei Wang, Qunyuan Xu, Zhiguo Chen
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2018)
Article
Multidisciplinary Sciences
Weiqi Zhang, Haifeng Wan, Guihai Feng, Jing Qu, Jiaqiang Wang, Yaobin Jing, Ruotong Ren, Zunpeng Liu, Linlin Zhang, Zhiguo Chen, Shuyan Wang, Yong Zhao, Zhaoxia Wang, Yun Yuan, Qi Zhou, Wei Li, Guang-Hui Liu, Baoyang Hu
Article
Critical Care Medicine
Yingli Jing, Degang Yang, Fan Bai, Chao Zhang, Chuan Qin, Di Li, Limiao Wang, Mingliang Yang, Zhiguo Chen, Jianjun Li
JOURNAL OF NEUROTRAUMA
(2019)
Article
Oncology
Yu Zhao, Zhongfeng Liu, Xuan Wang, Huantong Wu, Jianping Zhang, Junfang Yang, Fayou Zhang, Lining Liu, Jiafu Long, Peihua Lu, Zhiguo Chen
CLINICAL CANCER RESEARCH
(2019)
Article
Multidisciplinary Sciences
Wei Zheng, Zhiguo Chen
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Medicine, Research & Experimental
Rong Cai, Yu Zhang, Jacob E. Simmering, Jordan L. Schultz, Yuhong Li, Irene Fernandez-Carasa, Antonella Consiglio, Angel Raya, Philip M. Polgreen, Nandakumar S. Narayanan, Yanpeng Yuan, Zhiguo Chen, Wenting Su, Yanping Han, Chunyue Zhao, Lifang Gao, Xunming Ji, Michael J. Welsh, Lei Li
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Cell & Tissue Engineering
Heng Chi, Yunqian Guan, Fengyan Li, Zhiguo Chen
CELL TRANSPLANTATION
(2019)
Review
Neurosciences
Deqiang Han, Wei Zheng, Xueyao Wang, Zhiguo Chen
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)
Review
Cell & Tissue Engineering
Deqiang Han, Xin Zheng, Xueyao Wang, Tao Jin, Li Cui, Zhiguo Chen
STEM CELLS INTERNATIONAL
(2020)
Article
Cell & Tissue Engineering
Xiaobo Li, Wenxiu Yu, Yunqian Guan, Haiqiang Zou, Zhaohui Liang, Min Huang, Renchao Zhao, Chunsong Zhao, Zhenhua Ren, Zhiguo Chen
STEM CELLS INTERNATIONAL
(2020)
Article
Microbiology
Yingli Jing, Fan Bai, Limiao Wang, Degang Yang, Yitong Yan, Qiuying Wang, Yanbing Zhu, Yan Yu, Zhiguo Chen
Summary: FMT treatment has a significant impact on the microenvironment of spinal cord injury, involving microcirculation, blood-spinal cord-barrier, activation of immune cells, and secretion of neurotrophic factors. Metabolic profiling analysis reveals around 22 differentially detected metabolites between the groups, and beta-alanine was further chosen for functional validation experiments. Supplementation of beta-alanine significantly improves neuronal survival and the integrity of the blood-spinal cord-barrier at the lesion site, suggesting beta-alanine may be one of the mediators following FMT that contributes to the recovery.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Liping Zhou, Deqiang Han, Xingzhe Wang, Zhiguo Chen
Summary: This study provides evidence for the neuroprotective activities of human umbilical cord MSCs through anti-inflammatory actions. The study also suggests the potential of probiotic formulation VSL#3 as an adjuvant therapy in Parkinson's disease treatment, as it interacts with hMSCs to suppress inflammation and exert neuroprotective actions without altering the effects of hMSCs.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Huantong Wu, Yajie Yu, Yu Zhao, Weihua Liu, Zhongfeng Liu, Guangji Zhang, Zhiguo Chen
Summary: In this study, CD5-specific CAR-T cells were designed with different molecular structures and their anti-tumor efficacy against T cell acute lymphoblastic leukemia (T-ALL) was investigated in vitro and in vivo. The results showed that by optimizing the structure of CARs, the anti-tumor activity of CAR-T cells against T-ALL cells was enhanced. Specifically, CD5 CAR-T cells with a CD28 co-stimulation domain and C(H)2C(H)3 hinge region exhibited superior anti-tumor activity compared to other CAR designs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Geriatrics & Gerontology
Zhongfeng Liu, Xuan Wang, Kewen Jiang, Xunming Ji, Y. Alex Zhang, Zhiguo Chen
