Article
Cell & Tissue Engineering
Diego Noe Rodriguez-Sanchez, Giovana Boff Araujo Pinto, Luciana Politti Cartarozzi, Alexandre Leite Rodrigues de Oliveira, Ana Livia Carvalho Bovolato, Marcio de Carvalho, Jorge Vicente Lopes da Silva, Janaina de Andrea Dernowsek, Marjorie Golim, Benedito Barraviera, Rui Seabra Ferreira, Elenice Deffune, Mathues Bertanha, Rogerio Martins Amorim
Summary: The study demonstrated that the combination of 3D-printed PLC-NGCs with AdMSCs derived from adipose tissue exerted neuroregenerative effects on rats with sciatic nerve injury, improving motor function and nerve conduction.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Polymer Science
Ci Li, Meng Zhang, Song-Yang Liu, Feng-Shi Zhang, Teng Wan, Zhen-Tao Ding, Pei-Xun Zhang
Summary: This study demonstrated the effectiveness of constructing peripheral nerve conduits based on chitosan acetylation, showing good biocompatibility and mechanical properties. Culturing bone marrow mesenchymal stem cells (BMSCs) as spheroids improves therapeutic potential, with BMSCs spheroids exhibiting better results in the PNI rat model with small gap tubulization. Chitin nerve conduits with BMSCs spheroids provide a promising therapy option for peripheral nerve regeneration.
Article
Cell & Tissue Engineering
Qunzhou Zhang, Justin C. Burrell, Jincheng Zeng, Faizan Motiwala, Shihong Shi, D. Kacy Cullen, Anh D. Le
Summary: This study generated gingiva-derived mesenchymal stem cell (GMSC)-repopulated nerve protectors and evaluated their therapeutic effects in a crush injury model. The results showed that the Schwann-like cells converted from GMSCs were able to promote functional recovery and axonal regeneration of injured sciatic nerves.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Fernando Gisbert Roca, Luis Gil Santos, Manuel Mata Roig, Lara Milian Medina, Cristina Martinez-Ramos, Manuel Monleon Pradas
Summary: This study presents a novel multimodular nerve guidance conduit for the treatment of large nerve damages. The conduit consists of aligned PLA microfibers and HA conduits that retain pre-seeded auxiliary cells. The results demonstrate that this multimodular approach is effective for promoting nerve regeneration and provides new surgical solutions in this field.
Article
Chemistry, Multidisciplinary
Jio Kim, Jooik Jeon, Ju-Yong Lee, Badamgarav Khoroldulam, Sung-Geun Choi, Jae-Young Bae, Jung Keun Hyun, Seung-Kyun Kang
Summary: This study proposes an electroceutical platform that combines a fully biodegradable conductive nerve conduit and a wireless electrical stimulator to maximize the therapeutic effect on nerve regeneration. The biodegradable nerve conduit made of molybdenum microparticles and polycaprolactone (PCL) eliminates the negative effects of non-degradable implants, reducing the risk of surgical complications. The optimized electrical and mechanical properties of Mo/PCL conduits are achieved by controlling the amounts of Mo and tetraglycol lubricant. In in vivo experiments, the integrated strategy of a conductive Mo/PCL conduit with controlled therapeutic electrical stimulation significantly accelerates axon regeneration and shows a significant therapeutic effect based on functional recovery tests.
Article
Emergency Medicine
Taotao Hu, Shusen Chang, Fang Qi, Zhonghui Zhang, Jiayin Chen, Lingli Jiang, Dali Wang, Chengliang Deng, Kaiyu Nie, Guangchao Xu, Zairong Wei
Summary: This study investigated the role of exosomes derived from Schwann cell-like cells (SCLCs) in peripheral nerve regeneration. SCLCs-exo significantly enhanced motor function recovery, attenuated muscle atrophy, and facilitated axonal regrowth, myelin formation, and angiogenesis. Furthermore, SCLCs-exo efficiently promoted proliferation and migration of Schwann cells and upregulated the expression of neurotrophic factors, myelin regulators, and myelin proteins.
Article
Biotechnology & Applied Microbiology
Yingshuai Wang, Tao Yu, Feihu Hu
Summary: This study explores the application of miRNAs and exosomes in peripheral nerve regeneration and finds that a hypocapnia environment can enhance exosome function. By delivering miR-218 through engineered exosomes, sciatic nerve regeneration can be facilitated, and hypocapnia stimuli-responsive exosomes show better results.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Songyang Liu, Liping Zhou, Ci Li, Tiantian Min, Changfeng Lu, Shuai Han, Meng Zhang, Yongqiang Wen, Peixun Zhang, Baoguo Jiang
Summary: Our research demonstrates the potential of using DNA-peptide coated chitin conduits for peripheral nerve injury repair. The DNA-peptide coating promotes Schwann cell proliferation and secretion, endothelial cell proliferation, and axon outgrowth. In vivo experiments show that the DNA-peptide coated conduits facilitate nerve regeneration and functional recovery through angiogenesis and neurogenesis.
Article
Cell Biology
Julius M. Mayer, Christian Krug, Maximilian M. Saller, Annette Feuchtinger, Riccardo E. Giunta, Elias Volkmer, Thomas Holzbach
Summary: Recent research highlights the beneficial effects of hypoxia-preconditioned adipose-derived multipotent stem/progenitor cells (ADSPCs) in peripheral nerve regeneration. The study investigated the use of a custom-made fibrin conduit seeded with hypoxic pre-conditioned ADSPCs for autologous sciatic nerve graft in rats. Compared to other treatment modes, the hypoxic pre-conditioned group showed faster functional regeneration, increased axon outgrowth/branching and density, remyelination, and reduced connective tissue area. Further studies are needed to explore the cellular mechanism and potential clinical applications.
NEURAL REGENERATION RESEARCH
(2023)
Article
Engineering, Biomedical
Zonghuan Wang, Yibing Wu, Yang Xiang, Marie Beatrix Kruth, Peng Wei, Guangli Dai, Kedi Xu, Jun Yin, Yong Huang
Summary: The study illustrates that nerve conduits with micro-grooved inner textures significantly improve peripheral nerve regeneration, leading to enhanced nerve tissue repair efficiency. These conduits show similar repair capacity to autografts, demonstrating the potential for improved nerve tissue engineering strategies.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Bo Wang, Chang-Feng Lu, Zhong-Yang Liu, Shuai Han, Pi Wei, Dian-Ying Zhang, Yu-Hui Kou, Bao-Guo Jiang
Summary: A novel nerve graft composed of chitin scaffolds and a small autologous nerve was used to repair sciatic nerve defects in rats, and it effectively facilitated nerve regeneration and restored neurological function, suggesting its potential as a new option for tissue-engineered nerve scaffolds.
NEURAL REGENERATION RESEARCH
(2022)
Article
Engineering, Biomedical
Binghui Jin, Yunru Yu, Xiangxiang Chen, Yanhong Yang, Yushan Xiong, Young Jun Im, Yuanjin Zhao, Jian Xiao
Summary: The study proposes a novel microtube for sciatic nerve regeneration using gradient decellularized porcine sciatic nerve extracellular matrix hydrogel (pDScNM-gel). The pDScNM is shown to enhance cell proliferation and migration, improve axon growth, and these effects can also be achieved through coculture in a gradient pDScNM microtube. In vivo sciatic nerve regeneration and functional recovery are demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.
BIOACTIVE MATERIALS
(2023)
Article
Polymer Science
Nurjemal A. Tagandurdyyeva, Maxim A. Trube, Igor' O. Shemyakin, Denis N. Solomitskiy, German V. Medvedev, Elena N. Dresvyanina, Yulia A. Nashchekina, Elena M. Ivan'kova, Irina P. Dobrovol'skaya, Almaz M. Kamalov, Elena G. Sukhorukova, Olga A. Moskalyuk, Vladimir E. Yudin
Summary: New tubular conduits consisting of poly (L-lactide) nanofibers and chitosan composite fibers with chitin nanofibrils have been developed for peripheral nerve regeneration and repair. In vitro studies showed good biocompatibility with human embryonic bone marrow stromal cells (FetMSCs), with cell adhesion and differentiation observed just one day after cultivation. An in vivo study evaluating motor-coordination disorders using the sciatic nerve functional index (SFI) assessment demonstrated an increased regeneration rate of the sciatic nerve with the presence of chitosan monofibers and chitosan composite fibers with chitin nanofibrils in the conduit design, resulting in an improved SFI value ranging from 76 to 83.
Article
Biochemistry & Molecular Biology
Anjali Yadav, Thamil Selvee Ramasamy, Sheng-Che Lin, Szu-Han Chen, Jean Lu, Ya-Hsin Liu, Fu- Lu, Yuan-Yu Hsueh, Shau-Ping Lin, Chia-Ching Wu
Summary: The failure of peripheral nerve regeneration may be attributed to the lack of a permissive environment for nerve repair. The use of autologous platelet-rich plasma (PRP) or its derivative platelet-rich growth factors (PRGF) has been shown to improve peripheral nerve regeneration by modulating macrophage polarization. In this study, PRGF was found to reduce inflammation and promote the shift of macrophages from a pro-inflammatory M1 phenotype to an anti-inflammatory M2 phenotype. This led to improved nerve regeneration in a rat sciatic nerve transection model. The findings suggest that autologous PRGF has the potential to regulate inflammatory microenvironments and promote nerve repair.
Article
Anatomy & Morphology
Shuai Wei, Qian Hu, Xiaoqing Cheng, Jianxiong Ma, Xuezhen Liang, Jiang Peng, Wenjing Xu, Xun Sun, Gonghai Han, Xinlong Ma, Yu Wang
FRONTIERS IN NEUROANATOMY
(2020)
Article
Neurosciences
Yi-jun Liu, Xiao-feng Chen, Li-ping Zhou, Feng Rao, Dian-ying Zhang, Yan-hua Wang
Summary: This study aimed to analyze the effect and mechanism of Wnt5a-loaded fibrin hydrogel on a 10-mm rat sciatic nerve defect. Results showed that Wnt5a could promote Schwann cell proliferation and the expression of various neurotrophic factors, and the Wnt5a/Fn group outperformed the control groups in terms of nerve regeneration and functional recovery 12 weeks after the operation. The Wnt5a/Fn nerve conduit may promote peripheral nerve defect regeneration by facilitating multiple neurotrophin secretion, combining vascularization and neurotrophic growth cues.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Materials Science, Multidisciplinary
Mengjiao Ma, Guodong Liu, Haoye Meng, Yudong Zheng, Jiang Peng, Yajie Xie, Yansen Wang, Wei He, Shuai Wei
Summary: Filling conductive guiding cues into nerve conduits, using self-polymerized dopamine to prepare conductive microcarriers, has shown promising results in promoting nerve defect repair and demonstrating good potential in vivo.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Applied
Guodong Liu, Mengjiao Ma, Haoye Meng, Jintao Liu, Yudong Zheng, Jiang Peng, Shuai Wei, Yi Sun, Yansen Wang, Yajie Xie, Junfei Li
Summary: In this study, biocompatible and electroactive BC/PEDOT-SNFs composite membranes were prepared, which showed excellent conductivity and cell loading capacity, making them suitable for peripheral nerve repair.
CARBOHYDRATE POLYMERS
(2022)
Article
Orthopedics
Yuxuan Zhang, Tao Lin, Haoye Meng, Xueting Wang, Hong Peng, Guangbo Liu, Shuai Wei, Qiang Lu, Yu Wang, Aiyuan Wang, Wenjing Xu, Huiping Shao, Jiang Peng
Summary: This study successfully prepared a 3D gel-printed DCPD-coated porous magnesium scaffold and evaluated its biocompatibility, degradation rate, and osteogenic ability in vitro and in vivo. The results showed that the scaffold has great potential as a bone graft alternative.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Engineering, Multidisciplinary
Faxing Zou, Haoye Meng, Mengjiao Ma, Feng Han, Yansen Wang, Yajie Xie, Jiayu Yang, Shuai Wei, Emilia Choinska, Jiang Peng, Yudong Zheng
Summary: Due to disorder in pH, inflammation, and incomplete structure of cartilage-bone interface, repairing cartilage defects is a pressing issue. The proposed solution is to create a multifunctional cartilage scaffold that stabilizes pH, reduces inflammation, and promotes structural integrity in the joint. This study constructed a cartilage scaffold (M-APUs) using amino acid-based polyurethane and modified chitosan, which creates a favorable microenvironment, supports stem cell growth, and enhances osteochondral remodeling. Animal models confirmed that the M-APUs prevented cartilage deterioration, promoted repair, and improved joint structure and functionality. The synergistic strategy employed in this novel system has the potential to be a competitive biomaterial in cartilage damage treatment.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Shuai Wei, Yu Wang, Yu Sun, Leilei Gong, Xiu Dai, Haoye Meng, Wenjing Xu, Jianxiong Ma, Qian Hu, Xinlong Ma, Jiang Peng, Xiaosong Gu
Summary: Compared with most nondegradable or slowly degradable bone repair materials, bioactive biodegradable porous scaffolds with certain mechanical strengths can promote the regeneration of both new bone and vasculature while the cavity created by their degradation can be replaced by the infiltration of new bone tissue. A three-dimensional porous biomimetic composite scaffold with a two-component SF-MC system was constructed based on the advantages of mineralized collagen (MC) and silk fibroin (SF). The SF-MC scaffold showed good osteogenic induction and cell proliferation abilities, and in vivo experiments confirmed its efficacy in stimulating vascular and bone regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Shuai Wei, Qian Hu, Jianxiong Ma, Xiu Dai, Yu Sun, Gonghai Han, Haoye Meng, Wenjing Xu, Lei Zhang, Xinlong Ma, Jiang Peng, Yu Wang
Summary: Compared to conventional artificial nerve guide conduits, acellular nerve grafts derived from natural nerves have natural advantages. In this study, a composite technology based on supercritical carbon dioxide was used to process the peripheral nerve of a large mammal and obtain acellular nerve xenografts. The extracted grafts showed improved cell adhesion and proliferation, reduced inflammatory response, and promoted nerve regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Medicine, General & Internal
Shuai Wei, Wei Wang, Li Li, Hao-Ye Meng, Chun-Zhen Feng, Yu-Ying Dong, Xi-Chi Fang, Qi-Qiang Dong, Wen Jiang, Hai-Li Xin, Zhan-Zhen Li, Xin Wang
Summary: The study found that an EGF concentration of 10 ng/ml and an action time of 10 min had optimal effects on cell proliferation and migration, and the V + E 10 min group promoted wound healing to the greatest extent and obtained the best histological score.
MILITARY MEDICAL RESEARCH
(2021)
Review
Medicine, General & Internal
Shuai Wei, Jian-Xiong Ma, Lai Xu, Xiao-Song Gu, Xin-Long Ma
MILITARY MEDICAL RESEARCH
(2020)
Article
Cell Biology
Shuai Wei, Xue-Zhen Liang, Qian Hu, Wei-Shan Wang, Wen-Jing Xu, Xiao-Qing Cheng, Jiang Peng, Quan-Yi Guo, Shu-Yun Liu, Wen Jiang, Xiao Ding, Gong-Hai Han, Ping Liu, Chen-Hui Shi, Yu Wang
NEURAL REGENERATION RESEARCH
(2020)
Article
Cell Biology
Xiao-Qing Cheng, Xue-Zhen Liang, Shuai Wei, Xiao Ding, Gong-Hai Han, Ping Liu, Xun Sun, Qi Quan, He Tang, Qing Zhao, Ai-Jia Shang, Jiang Peng
NEURAL REGENERATION RESEARCH
(2020)
Review
Cell Biology
Ping Liu, Jiang Peng, Gong-Hai Han, Xiao Ding, Shuai Wei, Gang Gao, Kun Huang, Feng Chang, Yu Wang
NEURAL REGENERATION RESEARCH
(2019)
Review
Cell Biology
Gong-Hai Han, Jiang Peng, Ping Liu, Xiao Ding, Shuai Wei, Sheng Lu, Yu Wang
NEURAL REGENERATION RESEARCH
(2019)