Article
Agronomy
Mengxue Hu, Peifu Wu, Aiwei Guo, Lily Liu
Summary: This study investigates the regulatory mechanism of myristic acid on milk fat synthesis in cows. It reveals that myristic acid regulates milk fat synthesis through ubiquitination-lysosome and ubiquitination-proteasome pathways. The results demonstrate that myristic acid increases the protein expression levels of CD36, ADFP, and UB, as well as the content of triglyceride and lipid droplets in bovine mammary epithelial cells.
Article
Agriculture, Multidisciplinary
Jiangtao Huang, Jintao Li, Yong Ning, Yalun Ren, Yuexin Shao, Huawen Zhang, Xueyang Zong, Huaiping Shi
Summary: Leucine supplementation increases milk protein and milk fat synthesis in bovine mammary cells. It activates mTOR and increases the expression of key genes involved in lipid metabolism. PPAR alpha is independently correlated with leucine dosage and plays a role in leucine catabolism.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Wenying Zhang, Changhui Zhang, Jun Luo, Huifen Xu, Jianxin Liu, Juan J. Loor, Hengbo Shi
Summary: This study provides evidence of a physiological link between LXRB and SREBP1 in ruminant mammary cells, demonstrating their important role in the synthesis of PUFA by regulating genes encoding elongases and desaturases. Targeting this network may have broad effects on lipid homeostasis in the ruminant mammary gland.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Jie Xu, Xin-Ling Wang, Han-Fang Zeng, Zhao-Yu Han
Summary: In this study, the regulatory effect and mechanism of methionine (Met) in alleviating heat stress-induced ferroptosis in bovine mammary epithelial cells were investigated. The results showed that Met increased the expression levels of GPX4, SLC7A11, and FTH1 by activating Nrf2, resulting in a decrease in LIP and lipid ROS levels, thus alleviating heat stress-induced ferroptosis. These findings highlight the significant role of Met in mitigating bovine mammary gland injury caused by heat stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Zhi Chen, Xiang Cao, Qinyue Lu, Jingpeng Zhou, Yuhao Wang, Yanni Wu, Yongjiang Mao, Huifen Xu, Zhangping Yang
Summary: The study found that circ01592 promotes milk fat synthesis and increases the content of unsaturated fatty acids by binding to miR-218, while the function of miR-218 is opposite to that of circ01592. These findings provide a new research pathway for improving milk quality.
Article
Multidisciplinary Sciences
Theresa Casey, Aridany Suarez-Trujillo, Shelby Cummings, Katelyn Huff, Jennifer Crodian, Ketaki Bhide, Clare Aduwari, Kelsey Teeple, Avi Shamay, Sameer J. Mabjeesh, Phillip San Miguel, Jyothi Thimmapuram, Karen Plaut
Summary: The mammary epithelial circadian clock, regulated by BMAL1:CLOCK, plays a role in mammary gland development and lactation by controlling genes related to cell adhesion, membrane transport, and lipid metabolism. Knocking out BMAL1 in HC11 cells resulted in lower cell densities, increased reactive oxygen species, and decreased expression of genes associated with lactation, supporting the hypothesis that the clock is important in mammary development and substrate transport.
Article
Agriculture, Dairy & Animal Science
Tianle Xu, Run Liu, Xubin Lu, Xinyue Wu, Petr Heneberg, Yongjiang Mao, Qianming Jiang, Juan Loor, Zhangping Yang
Summary: This study investigated the effects of Lycium barbarum polysaccharides (LBP) on proinflammatory responses in lipopolysaccharide (LPS)-stimulated primary bovine mammary epithelial cells. The results showed that LBP reduced the expression of proinflammatory factors and cytokines induced by LPS stimulation, inhibited the NF-kappa B and MAPK pathways, and activated peroxisome proliferator-activated receptor-gamma (PPAR gamma). LBP also reversed the negative effect of LPS on cell proliferation. The protective effect of LBP on LPS-induced inflammation was found to be dependent on PPAR gamma activation.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Chaochao Luo, Wanwan Peng, Jia Kang, Caiming Chen, Jiajia Peng, Yue Wang, Qian Tang, Hailong Xie, Yazi Li, Xinghua Pan
Summary: This study demonstrates that glutamine (Gln) positively regulates cell growth and casein synthesis in bovine mammary epithelial cells (BMECs) through the CYTHs/ARFGAP1-Arf1-mTORC1 pathway, providing new insights into the regulation of the mTOR pathway by amino acids.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Baojun Yu, Jiamin Liu, Zhengyun Cai, Tong Mu, Di Zhang, Xiaofang Feng, Yaling Gu, Juan Zhang
Summary: MiR-19a plays a crucial role in regulating the proliferation and differentiation of bovine mammary epithelial cells (BMECs) and in biological processes related to triglyceride synthesis and lipid droplet formation by suppressing SYT1 expression.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Veterinary Sciences
Zhuo-Ma Luoreng, Da-Wei Wei, Xing-Ping Wang
Summary: Mastitis is a complex inflammatory disease in dairy cows caused by pathogenic infection, and miR-125b plays a crucial role in its occurrence and development. Downregulation of miR-125b can increase inflammatory factors production, while its upregulation can alleviate the inflammatory response.
VETERINARY RESEARCH
(2021)
Article
Agronomy
Xin Zhao, Jun Li, Shuying Zhao, Lili Chen, Man Zhang, Yi Ma, Dawei Yao
Summary: bta-miRNA29d-3p plays a crucial role in regulating mammary lipid synthesis in cows by modulating genes related to lipid metabolism and targeting GPAM.
Article
Multidisciplinary Sciences
Julia Armbruster, Mostafa A. Aboouf, Max Gassmann, Angela Egert, Hubert Schorle, Veit Hornung, Tobias Schmidt, Jonathan L. Schmid-Burgk, Glen Kristiansen, Anne Bicker, Thomas Hankeln, Hao Zhu, Thomas A. Gorr
Summary: Myoglobin expression in mammary epithelial cells promotes fatty acid oxidation and reduces lipogenesis. Presence of oxygenated myoglobin increases limited fatty acid beta-oxidation and alters fatty acid composition. Myoglobin also enhances cytoplasmic fatty acid solubility and deposition to lipid droplets. These findings reveal novel functions of myoglobin in mammary epithelial cells, including regulation of fatty acid metabolism and inflammatory signaling.
Article
Genetics & Heredity
Shuangfeng Chu, Yi Yang, Mudasir Nazar, Zhi Chen, Zhangping Yang
Summary: In this study, miR-497 was found to play an important role in fat metabolism by analyzing miRNA expression profiles in mammary gland tissues of cows at different lactation stages. MiR-497 mimics inhibited fat metabolism, while miR-497 knockdown promoted fat metabolism in bovine mammary epithelial cells. Further research revealed that miR-497 could down-regulate the expression of LATS1, which in turn influenced fatty acid, triacylglycerol, and cholesterol concentrations in cells.
Article
Agronomy
Xiaogang Cui, Tianqi Yuan, Zhengyu Fang, Jiao Feng, Changxin Wu
Summary: The study identified an miRNA, bta-miR-125a, that can control milk-fat production in cows by targeting the 3' untranslated region (UTR) of the SAA1 gene. The presence of miR-125a mimic decreased SAA1 expression and increased triglyceride and cholesterol content in bovine mammary epithelial cells.
Article
Cell Biology
Jessica Elswood, Scott J. Pearson, H. Ross Payne, Rola Barhoumi, Monique Rijnkels, Weston W. Porter
Summary: Through studying the differentiation and metabolism of mammary epithelial cells, it was found that mitophagy occurs selectively during the differentiation process. Inhibiting autophagy impairs cell differentiation, while the PRKN protein is necessary for cell differentiation.
Article
Biochemistry & Molecular Biology
Weipeng Wang, Qiburi He, Zhixin Guo, Limin Yang, Lili Bao, Wenlei Bao, Xu Zheng, Yanfeng Wang, Zhigang Wang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2015)
Article
Agriculture, Dairy & Animal Science
Zhixin Guo, Yanfeng Wang, Xue Feng, Chaogetu Bao, Qiburi He, Lili Bao, Huifang Hao, Zhigang Wang
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
(2016)
Article
Biotechnology & Applied Microbiology
Yuting Fu, Xu Zheng, Xiaoyang Jia, Uyanga Binderiya, Yanfeng Wang, Wenlei Bao, Lili Bao, Keyu Zhao, Yu Fu, Huifang Hao, Zhigang Wang
Article
Agriculture, Dairy & Animal Science
Xue Feng, Lili Bao, Manlin Wu, Di Zhang, Le Yao, Zhixin Guo, Dandan Yan, Pingping Zhao, Huifang Hao, Zhigang Wang
JOURNAL OF APPLIED ANIMAL RESEARCH
(2018)
Article
Agriculture, Dairy & Animal Science
Wenlei Bao, Jianxin Yin, Yan Liang, Zhixin Guo, Yanfeng Wang, Dongjun Liu, Xiao Wang, Zhigang Wang
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
(2014)
Article
Immunology
Qiang Ji, Meng Zhang, Yanfeng Wang, Yuhao Chen, Liping Wang, Xinyue Lu, Linfeng Bai, Manshulin Wang, Lili Bao, Huifang Hao, Zhigang Wang
Summary: This study found that chlorogenic acid (CGA) mitigates the inflammatory response caused by Staphylococcus aureus, protects milk protein synthesis, and improves amino acid uptake in bovine mammary epithelial cells (BMECs). CGA regulates the activity of mTORC1 and STAT5, upregulates IL-10 and IL-10RA, and reverses the inhibition of milk protein synthesis caused by Staphylococcus aureus.
MICROBIAL PATHOGENESIS
(2022)
Article
Cell Biology
Yanfeng Wang, Zhixin Guo, Mingtao Liu, Keyu Zhao, Xue Feng, Xu Zheng, Xiaojing Wang, Huifang Hao, Xudong Guo, Zhigang Wang
ANIMAL CELLS AND SYSTEMS
(2017)