Article
Polymer Science
Oana Gherasim, Alexandru Mihai Grumezescu, Valentina Grumezescu, Ecaterina Andronescu, Irina Negut, Alexandra Catalina Birca, Bianca Galateanu, Ariana Hudita
Summary: Osteoconductive and osteoinductive coatings are effective strategies for enhancing the integration of metallic implants with bone. Composite materials based on PLA and HAp benefit bone cell development, while the addition of osteogenic proteins contributes to new bone formation. Laser-processed composites show superior ability to promote osteoprogenitor cell adhesion, viability, and proliferation.
Article
Biotechnology & Applied Microbiology
Gaoli Xu, Chenxi Shen, Haiyan Lin, Jian Zhou, Ting Wang, Ben Wan, Munerah Binshabaib, Tymour Forouzanfar, Guochao Xu, Nawal Alharbi, Gang Wu
Summary: This study synthesized a novel biomimetically-precipitated nanocrystalline calcium phosphate (BpNcCaP) with internally incorporated bone morphogenetic protein-2 (BpNcCaP + BMP-2), demonstrating its potential application in repairing large-volume bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Sihan Lin, Shi Yin, Junfeng Shi, Guangzheng Yang, Xutao Wen, Wenjie Zhang, Mingliang Zhou, Xinquan Jiang
Summary: In this study, the dose of bone morphogenetic protein-2 (BMP-2) was reduced and its safety and efficacy were improved through the regulation of magnesium ion (Mg2+). Magnesium ion enhanced cellular bioenergetic levels and the osteoinductivity of BMP-2. A co-delivery system, fabricated using microfluidic and 3D printing technologies, was able to rapidly and robustly induce bone regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Yang Liu, Manoj Puthia, Eamon J. Sheehy, Ines Ambite, Jitka Petrlova, Sujeethkumar Prithviraj, Maria Wimer Oxborg, Sujeesh Sebastian, Corina Vater, Stefan Zwingenberger, Andre Struglics, Paul E. Bourgine, Fergal J. O'Brien, Deepak Bushan Raina
Summary: This study compared the osteoinductive potential of BMP-2 ho-modimer with a heterodimer of BMP-2/7 in a collagen-hydroxyapatite (CHA) scaffold delivery system. The results showed that CHA + BMP-2/7 had higher osteoinductive ability than CHA + BMP-2, and the CHA scaffold provided sustained release of BMP-2/7 for at least 20 days. Additionally, a low dose of BMP-2/7 accelerated fracture healing and performed at a level comparable to a 20-times higher BMP-2 dose, bringing hope for the use of physiological growth factor doses in fracture healing.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Yuan-Zhe Jin, Guang-Bin Zheng, Jae Hyup Lee, Shi-Huan Han
Summary: In this study, rat calvaria defect model was used to compare the bone regeneration effect of ErhBMP-2 implanted with DBM or HA as carriers. The group with DBM as the carrier showed significantly higher bone volume and thickness in both weeks, indicating that DBM could be a better carrier for ErhBMP-2 than HA.
BIOMATERIALS RESEARCH
(2021)
Article
Engineering, Biomedical
Seoyun Lee, Honghyun Park, Jeong-Seop Oh, Kyubin Byun, Dae-Yong Kim, Hui-suk Yun, Byung-Jae Kang
Summary: Hydroxyapatite (HAp) microbeads loaded with both BMP-2 and Qct showed enhanced osteogenic potential compared to HAp loaded with BMP-2 or Qct alone. The electrostatic spraying method was found to be an efficient strategy for producing homogenous ceramic granules. These findings suggest that BMP-2 and Qct loaded HAp microbeads can serve as effective implants for bone defect healing.
BIOMEDICAL ENGINEERING ONLINE
(2023)
Article
Clinical Neurology
Zeynep Bal, Feza Korkusuz, Hiroyuki Ishiguro, Rintaro Okada, Junichi Kushioka, Ryota Chijimatsu, Joe Kodama, Daisuke Tateiwa, Yuichiro Ukon, Shinichi Nakagawa, Eda Ciftci Dede, Merve Gizer, Petek Korkusuz, Hideki Yoshikawa, Takashi Kaito
Summary: The novel composite material of nHAp/PLA-PEG demonstrated efficient bone regeneration with low-dose BMP-2. ELISA results showed sustained release of BMP-2 up to day 21, with 91.6% of specimens in both the low- and high-dose groups achieving solid fusion according to μCT and manual palpation test, while none in the control group achieved bony fusion.
Article
Biochemistry & Molecular Biology
Magdalena Wytrwal, Malgorzata Sekula-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba-Surma, Szczepan Zapotoczny, Krzysztof Szczubialka
Summary: This study investigated the covalent binding of recombinant human bone morphogenetic protein-2 and -7 in stem cells and its effects on cell adhesion, migration, and osteogenesis. The presence of both proteins enhanced cell adhesion but limited cell motility, while the presence of single proteins promoted cell migration activity. Additionally, the coexistence of the proteins significantly increased early osteogenic marker expression.
Article
Engineering, Biomedical
Sarah A. Schoonraad, Alan A. Jaimes, Arjun J. X. Singh, Kiera J. Croland, Stephanie J. Bryant
Summary: This study investigates the osteogenic effects of tethered BMP-2 and BMP-9 on human MSCs encapsulated in a hydrogel. The results show that tethered BMP-2 and BMP-9 both effectively enhance osteogenesis of MSCs, leading to a mature osteoblast phenotype.
ACTA BIOMATERIALIA
(2023)
Article
Nanoscience & Nanotechnology
Zhangyi Li, Peicai Liu
Summary: In this study, a hydroxyapatite-bone morphogenetic protein-2 (HA-BMP-2) nanocomposite was constructed and shown to have the potential for promoting periodontal bone regeneration. The results demonstrated that nHA-BMP-2 enhanced the activity and migration of HPLSCs and promoted bone defect reconstruction. Additionally, nHA-BMP-2 increased the mineralization of HPLSCs, thereby enhancing osteogenesis. Animal experiments further confirmed the effectiveness of nHA-BMP-2 in improving new bone formation in periodontal bone defect areas.
Article
Materials Science, Biomaterials
M. C. Echave, I Erezuma, N. Golafshan, M. Castilho, F. B. Kadumudi, C. Pimenta-Lopes, F. Ventura, A. Pujol, J. J. Jimenez, J. A. Camara, R. Hernaez-Moya, L. Iturriaga, L. Saenz Del Burgo, I. Iloro, M. Azkargorta, F. Elortza, R. Lakshminarayanan, T. H. Al-Tel, P. Garcia-Garcia, R. Reyes, A. Delgado, C. Evora, J. L. Pedraz, A. Dolatshahi-Pirouz, G. Orive
Summary: In this study, a new osteoconductive scaffold was developed, which reduced the required doses of BMP-2 for bone regeneration. The scaffold showed promising results in promoting cell adhesion, proliferation, and osteogenic differentiation of human bone-marrow derived mesenchymal stem cells (hBM-MSCs). The sustained release of BMP-2 from the scaffold allowed for the treatment of critical-sized osteoporotic bone defects with relatively low growth factor doses.
BIOMATERIALS ADVANCES
(2022)
Review
Chemistry, Multidisciplinary
Anis Syauqina Mohd Zaffarin, Shiow-Fern Ng, Min Hwei Ng, Haniza Hassan, Ekram Alias
Summary: Nano-hydroxyapatite (nHA) is effective in promoting bone regeneration as a delivery system, inducing bone regeneration successfully and enhancing osteogenesis when conjugated with other bioactive molecules.
Article
Biochemistry & Molecular Biology
Hyun Seok, Hee-Youl Kim, Dong-Cheol Kang, Jung-Ho Park, Jong Hoon Park
Summary: The study compared the bone regeneration ability of bone scaffolds using particle and block bones with rhBMP-2, showing that rhBMP-2 with block bones resulted in significantly higher bone volume but also led to the formation of adipose tissue and void spaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ho-Kyung Lim, Yeh-Jin Kwon, Seok-Jin Hong, Hyo-Geun Choi, Sung-Min Chung, Byoung-Eun Yang, Jong-Ho Lee, Soo-Hwan Byun
Summary: The study evaluated the effects of different concentrations of PDRN and rhBMP-2 on bone regeneration and mechanical properties of HA/TCP scaffolds. Results showed that rhBMP-2 significantly increased bone formation, while PDRN showed no significant effect; when used at optimal concentrations, HA/TCP scaffolds demonstrated sufficient bone regeneration capacity and mechanical strength.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Jonathan Dorogin, Henry B. B. Hochstatter, Samantha O. O. Shepherd, Justin E. E. Svendsen, Morrhyssey A. A. Benz, Andrew C. C. Powers, Karly M. M. Fear, Jakob M. M. Townsend, James S. S. Prell, Parisa Hosseinzadeh, Marian H. H. Hettiaratchi
Summary: By using yeast surface display, unique BMP-2-specific affibodies were identified to modulate the release and activity of BMP-2, offering a promising approach for controlling BMP-2 delivery in clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
