Article
Microbiology
Jiun-Nong Lin, Chung-Hsu Lai, Shang-Yi Lin, Ching-Chi Lee, Nan-Yao Lee, Po-Yu Liu, Chih-Hui Yang, Yi-Han Huang
Summary: This study evaluated the impact of intraspecific diversity among the multiple copies of the 16S rRNA gene on the accuracy of species identification in the genus Elizabethkingia. The results suggest that 16S rRNA gene sequencing should not be considered the gold standard for the accurate identification of Elizabethkingia species.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medical Laboratory Technology
Young-Gon Kim, Kiwook Jung, Seunghwan Kim, Man Jin Kim, Jee-Soo Lee, Sung-Sup Park, Moon-Woo Seong
Summary: SnackNTM is a simple and fully automated software that enables species-level identification of nontuberculous mycobacteria (NTM) from trace files. It has high performance and accuracy, and reduces the workload for NTM identification, especially in clinical laboratories processing large numbers of cases.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Fisheries
Yaxin Wu, Yibin Yang, Huifeng Dang, Hehe Xiao, Wenji Huang, Zhao Jia, Xin Zhao, Kangyong Chen, Ning Ji, Jiahong Guo, Zhiwei Qin, Junya Wang, Jun Zou
Summary: The study isolated a pathogenic strain of Klebsiella pneumoniae from a diseased spotted gar and explored its virulence and drug resistance. Results showed that fish infected with K. pneumoniae exhibited decreased immune cells and tissue inflammation.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Microbiology
Elisabete Alves Cappelli, Magdalena Ksiezarek, Jacqueline Wolf, Meina Neumann-Schaal, Teresa Goncalves Ribeiro, Luisa Peixe
Summary: The genus Corynebacterium is frequently found in the female urinary microbiome, but species-level characterization of Corynebacterium has been underexplored. In this study, eight strains isolated from urine samples were characterized and identified as potentially new Corynebacterium species.
Article
Microbiology
Satomi Takei, Yu Jie Lu, Mari Tohya, Shin Watanabe, Shigeki Misawa, Yoko Tabe, Takashi Miida, San Mya, Htay Htay Tin, Tatsuya Tada, Teruo Kirikae
Summary: This study demonstrates the spread of carbapenem-resistant K. pneumoniae strains in medical settings in Myanmar. Specifically, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in high-risk clones of K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Katerina Roth, Yadwinder Singh Rana, Devin Daeschel, Jasna Kovac, Randy Worobo, Abigail B. Snyder
Summary: Six thermo-acidophilic, spore-forming strains were isolated from juice products, representing three novel species of Alicyclobacillus. These strains can grow aerobically in acidic and moderate thermal conditions, with only two of them capable of producing guaiacol.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Immunology
Suat Moi Puah, Sam Pei Fong, Boon Pin Kee, S. D. Puthucheary, Kek Heng Chua
Summary: This study re-evaluated 30 archived Flavobacterium meningosepticum isolates and identified them as either E. anophelis or E. miricola using 16S rRNA gene sequencing, ERIC-PCR, and biofilm formation assay. The results showed that E. anophelis had a higher ability to form biofilm compared to E. miricola. Additionally, E. anophelis from tracheal secretions were significantly associated with strong biofilm formation. This suggests that E. anophelis may play a significant role in the pathogenesis of Elizabethkingia infections.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Ramesh Chandra Malick, Basanta Kumar Das, Bibhuti Bhusan Pal, Asit Kumar Bera, Praveen Maurye, Amiya Kumar Sahoo
Summary: Globally, bacterial gill disease is a major problem for marine and freshwater fishes. This study focused on diseased Labeo rohita, Labeo catla and Oreochromis niloticus with gill discoloration, tail rot and skin ulcers, which are typical signs of bacterial gill disease, in East Kolkata Wetlands, India. Four isolates of Chryseobacterium gleum were identified as the responsible pathogens, and they showed complete resistance to multiple antibiotics. A challenge experiment confirmed the virulence of three isolates.
Article
Microbiology
Jonas Greve Lauritsen, Morten Lindqvist Hansen, Pernille Kjersgaard Bech, Lars Jelsbak, Lone Gram, Mikael Lenz Strube
Summary: A high-throughput sequencing method was developed for profiling Pseudomonas species in soil microbiomes, identifying more species than 16S rRNA gene sequencing or cultivation. This method allows for tracing specific species of Pseudomonas and screening environmental samples for further isolation and exploitation.
Article
Infectious Diseases
Jin Zhao, Bin Lu, Yangxiao Zhou, Sipei Wang, Xinling Pan
Summary: M. seoulense strains can cause infection in humans, and they can be identified by sequencing ITS, hsp65, and rpoB genes.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Biology
Sehrish Ashraf, Asia Parveen, Muhammad Asif, Abdullah D. Alanazi, Abdulaziz Alouffi, Mian Muhammad Awais, Adil Khan, Munir Aktas, Sezayi Ozubek, Furhan Iqbal
Summary: Anaplasma centrale was found to have seasonal prevalence in cattle in District Layyah, Punjab, Pakistan, with the highest prevalence in spring. Sahiwal breed was most susceptible to A. centrale infection. Recommended screening for effective control of tick-borne diseases in Pakistan.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Fatemeh Movahedi-Parizi, Akbar Hosseinipour, Mehdi Azadvar, Hossain Massumi
Summary: Phytoplasma-related symptoms were found on plants in urban green spaces in Kerman province, southeastern Iran. PCR and sequencing were used to identify the phytoplasma strains and subgroups infecting the symptomatic plants, leading to the discovery of new infections.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Infectious Diseases
Mitsunori Tohda, Ken-Ichi Oinuma, Arata Sakiyama, Taishi Tsubouchi, Mamiko Niki, Hiroki Namikawa, Kenshi Yamane, Koichi Yamada, Tetsuya Watanabe, Kazuhisa Asai, Hiroshi Kakeya, Yukihiro Kaneko, Tomoya Kawaguchi
Summary: This study revealed that rifampicin exerts anti-mucoviscous activity by binding to RpoB and inhibiting rmpA. Mutations in RpoB confer high resistance to rifampicin, while revertants with wild-type RpoB show similar responses to rifampicin as the wild type. Transcription levels of rmpA and magA, and capsule thickness are correlated with mucoviscosity levels.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Melanie Roch, Roberto Sierra, Kirsty Sands, Willames M. B. S. Martins, Jacques Schrenzel, Timothy R. Walsh, Ana C. Gales, Diego O. Andrey
Summary: The study found that a significant portion of KPC-producing Klebsiella pneumoniae isolates in a Brazilian hospital exhibited resistance to common aminoglycosides like gentamicin and amikacin, with the presence of the rmtB gene in 30% of the strains. The rmtB gene was mainly found in sequence type 258 (ST258) and a novel ST16 clone, carried on large IncC plasmids. Furthermore, most isolates remained susceptible to the veterinary approved drug apramycin. The dissemination of rmtB in an endemic setting for KPC-producing clones is concerning, as it confers resistance to clinically available aminoglycosides, including the novel plazomicin.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Environmental Sciences
Xinyu Fang, Shen Lin, Yuting Zhang, Zonghua Wang, Konglin Zhou, Jianming Chen
Summary: A new species of Cladonema, named Cladonema digitatum sp. nov., was identified in a laboratory aquarium in Fuzhou, China, based on its morphological characteristics and DNA barcoding. It has distinct features that differentiate it from other Cladonema medusae. However, species identification based solely on morphology is difficult due to the phenotypic plasticity displayed by C. digitatum. Therefore, updating information on the life cycle and DNA barcoding is necessary to improve the taxonomy of the genus Cladonema.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Perry L. Wood, Xianguang Guo, Scott L. Travers, Yong-Chao Su, Karen Olson, Aaron M. Bauer, L. Lee Grismer, Cameron D. Siler, Robert G. Moyle, Michael J. Andersen, Rafe M. Brown
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2020)
Article
Genetics & Heredity
Bo Cai, Xianguang Guo, Zhaobin Song, Jianping Jiang
MITOCHONDRIAL DNA PART B-RESOURCES
(2020)
Article
Genetics & Heredity
Bo Cai, Xianguang Guo, Jianping Jiang
MITOCHONDRIAL DNA PART B-RESOURCES
(2020)
Article
Evolutionary Biology
Yuchi Zheng, Qiang Dai, Xianguang Guo, Xiaomao Zeng
BMC EVOLUTIONARY BIOLOGY
(2020)
Article
Genetics & Heredity
Minli Chen, Jinlong Liu, Dali Chen, Xianguang Guo
Article
Zoology
Minli Chen, Jinlong Liu, Bo Cai, Jun Li, Na Wu, Xianguang Guo
Summary: A new sand snake species, Psammophis turpanensis, was discovered in the Turpan Basin in northwest China, showing significant genetic and morphological differences from known Psammophis species. The study provides detailed descriptions of this new species and sheds light on the biogeography of Psammophis in China.
Article
Genetics & Heredity
Na Wu, Jinlong Liu, Song Wang, Xianguang Guo
Summary: In this study, the mitochondrial genomes of different subspecies of the sunwatcher toad-headed agama were analyzed to investigate intraspecific gene rearrangements. The study revealed prevalent gene rearrangement within the genus Phrynocephalus and identified two types of rearrangement in the mitochondrial genomes of P. helioscopus subspecies. The findings suggest that these rearrangements can occur rapidly through tandem duplication and random loss events. The study emphasizes the need for further research on mitogenomics at the population level.
Article
Genetics & Heredity
Lili Tian, Xianguang Guo
Summary: This study compared the mitochondrial genomes of individuals from the Eremias genus with other lacertids, and found that the genomes consisted of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding control region. The study also explored the composition of conserved structural elements in different genera of Lacertidae, and revealed the relationship between mitochondrial genomes and phylogeny.
Article
Genetics & Heredity
Mingrui Ma, Zhengnan Li, Xianguang Guo, Bin Zhang
Summary: The complete mitochondrial genome of the leafhopper Metidiocerus impressifrons was determined using next-generation sequencing. Phylogenetic analysis with 27 other species' mitochondrial genomes provides insights into the evolution of Idiocerinae mitochondrial genome.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Genetics & Heredity
Song Wang, Jinlong Liu, Marina A. Chirikova, Bin Zhang, Xianguang Guo
Summary: The complete mitogenome of the Yarkand racerunner has been determined for the first time using next-generation sequencing. Phylogenetic analysis revealed a close relationship between the Yarkand racerunner and E. przewalskii.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Genetics & Heredity
Xianguang Guo, Xiaopeng Huo, Jinlong Liu, Marina A. Chirikova
Summary: The complete mitogenome of the Kyrghyz racerunner from Kyrgyzstan was determined for the first time using next-generation sequencing. The mitogenome contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. Phylogenetic analysis revealed the monophyly of Eremias and its viviparous group, while the subgenus Pareremias was found to be paraphyletic.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Genetics & Heredity
Lili Tian, Wenxin Yang, Chengyan Si, Xianguang Guo, Bin Zhang
Summary: Insect mitochondrial genomes play a significant role in understanding molecular evolution, phylogenetics, and biogeography. This study determined the complete mitochondrial genomes of five species from the Idiocerini tribe using next-generation sequencing. The analysis provided insights into the mitochondrial genome structure and selective pressure of protein-coding genes. The findings revealed differences in gene expression among species and highlighted the prevalence of specific codons. Furthermore, the identification of tandem repeats and motifs suggests the need for more mitochondrial genomes in the Cicadellidae family.
Article
Biodiversity Conservation
Daniya Ualiyeva, Oleg A. Ermakov, Spartak N. Litvinchuk, Xianguang Guo, Alexander Yu Ivanov, Rui Xu, Jun Li, Feng Xu, Irina I. Arifulova, Alyona G. Kaptyonkina, Victor A. Khromov, Vladimir N. Krainyuk, Fakhriddin Sarzhanov, Tatjana N. Dujsebayeva
Summary: This study investigated the diversity, phylogenetic relationships, and distribution of marsh frogs in Kazakhstan and northwest China. Phylogenetic analysis revealed three main lineages and provided insights into the lineage diversification and population dynamics of the Central Asian marsh frogs.
Article
Genetics & Heredity
Na Wu, Bo Cai, Minli Chen, Xianguang Guo
MITOCHONDRIAL DNA PART B-RESOURCES
(2020)
Article
Genetics & Heredity
Dali Chen, Xiuliang Su, Jinlong Liu, Xianguang Guo
MITOCHONDRIAL DNA PART B-RESOURCES
(2019)