期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 110, 期 2, 页码 187-194出版社
SPRINGER
DOI: 10.1007/s10482-016-0789-z
关键词
Polyphasic approach; Proteobacteria; Pseudoruegeria marinistellae sp nov.; Starfish
类别
资金
- National High Technology Research and Development Program of China (863 Program) [2013AA092902, 2014AA093515]
- Introduction Project of Academic and Technology Leaders in Anhui University [32030066]
A taxonomic study was carried out on a Gram-stain negative, rod-shaped, non-flagellated, and facultatively anaerobic bacterial strain designated as strain SF-16(T), which was isolated from an unidentified starfish in Sanya, China. Strain SF-16(T) was found to be 5.0-7.0 mu m long, and oxidase and catalase positive. Cell growth was observed at pH 6.0-8.5 (optimum, 7.0-8.0), temperatures of 10-41 A degrees C (optimum, 25-30 A degrees C), and salinities of 0-12 % (optimum, 3.0-6.0 %). The predominant fatty acids (> 20 %) were found to be C-18:1 omega 7c and/or C-18:1 omega 6c (summed feature 8). Ubiquinone 10 was identified as the predominant quinone for strain SF-16(T). The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unidentified lipids, and three unidentified phospholipids. The DNA G+C content of the genomic DNA of strain SF-16(T) was determined to be 63.0 mol%. Phylogenetic analysis based on the 16S rRNA gene showed that strain SF-16(T) belongs to the genus Pseudoruegeria and is closely related to Pseudoruegeria sabulilitoris GJMS-35(T) (98.42 % similarity). The ANI value between strain SF-16(T) and P. sabulilitoris GJMS-35(T) was found to be 74.98 %, and DNA-DNA hybridization value was 21.1 +/- 2.3 % in silico and 57 % in vitro. Based on the low level of the genetic relatedness, phylogenetic and phenotypic data, a novel species Pseudoruegeria marinistellae sp. nov. is proposed. The type strain is SF-16(T) (=MCCC 1K01155(T) = KCTC 42910(T)).
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