Article
Microbiology
Penghao Lv, Xuesong Zhang, Mengze Song, Guijuan Hao, Fangkun Wang, Shuhong Sun
Summary: In this study, we constructed a recombinant Bacillus subtilis that can secrete a multi-epitope protein. Oral immunization of chickens with this recombinant B. subtilis induced high levels of specific IgG and specific secretory immunoglobulin A (sIgA), as well as increased levels of IL-2 and T cell-mediated immunity. This recombinant B. subtilis effectively protected chickens against S. Enteritidis and reduced pathological damage to the spleen and jejunum, indicating its potential as a mucosal vaccine candidate for animals to defend against S. Enteritidis in the future.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Ying Zhang, Ruo Mo, Sheng Sun, Zhanding Cui, Bo Liang, Entao Li, Tiecheng Wang, Ye Feng, Songtao Yang, Feihu Yan, Yongkun Zhao, Xianzhu Xia
Summary: Rabies can be effectively prevented and controlled in domestic dogs, cats, and some pets by intramuscular injection of vaccine. However, it is difficult to prevent inaccessible animals, especially stray dogs and wild animals, with intramuscular injection. Therefore, the development of a safe and effective oral rabies vaccine is necessary.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Seunghyun Sim, Yue Hui, David A. Tirrell
Summary: The incorporation of living cells into materials presents both challenges and possibilities. In this study, a new class of 3D-printable materials based on poly(N-hydroxymethylacrylamide) is developed, which allows the cross-linking of spore-forming bacteria into a polymeric scaffold. These embedded cells and spores can become metabolically active and produce proteins after re-swelling in nutrient-rich media. The leak-free scaffold enables protein production while preventing cell escape. This research demonstrates the potential utility of living composite materials in various applications.
Article
Veterinary Sciences
Jian Lin, Chunxiao Mou, Shuai Zhang, Liqi Zhu, Yuchen Li, Qian Yang
Summary: In this study, we developed an oral vaccine delivery system using Bacillus subtilis to express a porcine epidemic diarrhoea virus (PEDV) antigen. The system successfully enhanced the mucosal immune response and increased resistance to PEDV.
VETERINARY SCIENCES
(2022)
Article
Fisheries
Shujie Xu, Qi Wang, Fei Wang, Xiang Li, Baikui Wang, Yuanhao Zhou, Peng Zou, Li Tang, Dongyou Yu, Weifen Li
Summary: This study evaluated the effects of the probiotic Bacillus subtilis B10 on intestinal health and immune function in Chinese soft-shelled turtles. The results showed that B. subtilis B10 administration increased levels of urea nitrogen and IL-1 beta, as well as the activities of alkaline phosphatase, lactic dehydrogenase, and antioxidant enzymes in the turtles' serum. The probiotic also up-regulated the expression of genes related to intestinal tight junction protein and TLR8, improving intestinal health and immune function. Furthermore, RNA-Seq results indicated that TLR5 expression was significantly increased in the liver, suggesting a potential mechanism for the probiotic's immune-enhancing effects.
Article
Fisheries
Quan Wang, Xia Liang, Yunshang Ning, Shijia Liu, Zengjian Liang, Zemiao Zhang, Yingjing Chen, Jinqiao Cao, Fubao Wang, Lingfeng Lan, Guangzhao Cheng, Youhua Huang, Yan Huang, Qiwei Qin, Sheng Zhou
Summary: This study successfully constructed the CotC-LMBV recombinant B. subtilis spores and evaluated its protective efficacy and immune responses. The results showed that largemouth bass orally vaccinated with CotC-LMBV spores had a survival rate of 53.3% and a relative percent survival of 45.0% compared to the control group. The specific IgM level in serum and the expression of immune-related genes in the spleen were also significantly increased in the vaccinated group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agricultural Engineering
Xuyang Zhu, Kang Zhang, Hui Luo, Jing Wu
Summary: The bottleneck of recombinant protein production in microbial cell factories is sometimes caused by limited manipulable targets and a lack of gene annotation related to protein expression. In this study, the novel functions of PonA in Bacillus subtilis recombinant protein expression were described, and the mechanism of its chaperone activity was analyzed. Overexpression of PonA led to a significant increase in the expression of hyperthermophilic amylase in shake flasks and fed-batch processes. Increased cell diameter and reinforced cell walls were observed in PonA-overexpressing strains. The FN3 structural domain and the natural dimeric structure of PonA may play critical roles in its chaperone activity. These findings suggest that PonA can be an effective target for modifying the expression of recombinant proteins in B. subtilis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Immunology
Johnny Chun-Chau Sung, Ying Liu, Kam-Chau Wu, Man-Chung Choi, Chloe Ho-Yi Ma, Jayman Lin, Emily Isabel Cheng He, David Yiu-Ming Leung, Eric Tung-Po Sze, Yusuf Khwaja Hamied, Dominic Man-Kit Lam, Keith Wai-Yeung Kwong
Summary: An engineered Bacillus subtilis spore expressing the SARS-CoV-2 spike protein receptor binding domain on the spore surface was developed and showed significant increases in neutralizing antibody after oral administration, with no adverse health events observed. These findings provide a basis for further clinical development of Bacillus subtilis spores as an oral vaccine candidate against COVID-19 in the future.
Article
Biotechnology & Applied Microbiology
Silja Vaelimets, Patricia Pedetti, Ludovika Jessica Virginia, Mai Ngoc Hoang, Michael Sauer, Clemens Peterbauer
Summary: Small laccases from Streptomyces coelicolor, Streptomyces viridosporus, and Amycolatopsis 75iv2 were successfully expressed in both Gram-positive expression hosts B. subtilis and S. lividans. However, the extracellular activities of the laccases were significantly higher in S. lividans compared to B. subtilis.
MICROBIAL CELL FACTORIES
(2023)
Article
Agriculture, Multidisciplinary
Shang-Ta Wang, Jou-An Chen, Chen Hsu, Nan-Wei Su
Summary: The study generated two water-soluble derivatives of Hst, Hst 7-O-phosphate and Hst3'-O-phosphate, with enhanced intestinal permeability and bioavailability, showing potential as efficient alternatives to Hst.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Zifan Chen, Chaojun Zheng, Qiaoli Peng, Qingfeng Zhou, Yunping Du, Huihua Zhang
Summary: In this study, Bacillus subtilis was used to express the anti Eimeria tenella surface protein SAG22. The results showed successful intracellular expression of the recombinant protein. Animal experiments demonstrated that the recombinant strain SAG22-DH61 had good immunoprotective effects and showed high coccidia resistance in broiler chickens.
Article
Microbiology
Aysegul Oktem, David Nunez-Nepomuceno, Borja Ferrero-Bordera, Jonathan Walgraeve, Michael Seefried, Manuela Gesell Salazar, Leif Steil, Stephan Michalik, Sandra Maass, Doerte Becher, Ulrike Maeder, Uwe Voelker, Jan Maarten van Dijl
Summary: This study reveals that modulation of the protease activity of HtrA, rather than complete removal, can limit the effects of secretion stress and improve bacterial fitness and enzyme yields.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Xi-Yuan Cao, Reyihanguli Aimaier, Jun Yang, Jing Yang, Zhong-Yi Chen, Jing-Jing Zhao, Li Yin, Qi Zhang, Jia You, Hui Zhang, Hao-Ran Li, Jia-Yi Chen, Qing-Chen Mao, Li-Ping Yang, Fei Yu, He-Ping Zhao, Hui-Xin Zhao
Summary: It has been found that the secondary metabolites of Bacillus subtilis strain Z15 can enhance immune function in mice, showing potential in treating fungal infections. Blood transcriptome analysis revealed the molecular mechanism and immune-related pathways involved. These findings provide a theoretical foundation for the development and application of immunotherapeutic approaches.
Article
Immunology
Ming-Shu Hsieh, Mei-Yu Chen, Chia-Wei Hsu, Yu-Wen Tsai, Fang-Feng Chiu, Cheng-Lung Hsu, Chang-Ling Lin, Chiao-Chieh Wu, Ling-Ling Tu, Chen-Yi Chiang, Shih-Jen Liu, Ching-Len Liao, Hsin-Wei Chen
Summary: Formyl peptide receptor-like 1 inhibitor protein (FLIPr) is a potential vaccine candidate produced by Staphylococcus aureus for reducing its virulence and biofilm formation. Recombinant lipidated FLIPr (rLF) was produced to increase its immunogenicity and showed to elicit potent anti-FLIPr responses and overcome FLIPr-mediated inhibition of phagocytosis. Additionally, rLF acts as an effective adjuvant by inducing strong and long-lasting immune responses in mice, supporting its potential use to enhance vaccine efficacy and abolish FLIPr-mediated immunosuppressive effects.
Review
Biotechnology & Applied Microbiology
Haiquan Yang, Jinfeng Qu, Wei Zou, Wei Shen, Xianzhong Chen
Summary: This article comprehensively reviews recent studies on recombinant protein production in Bacillus subtilis, focusing on strain optimization, expression enhancement, secretion improvement, surface display of proteins, and fermentation optimization. Novel methods and future challenges for recombinant protein production in B. subtilis are proposed.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
