Article
Biochemistry & Molecular Biology
Hsia-Wei Liu, Wen-Ta Su, Ching-Yi Liu, Ching-Cheng Huang
Summary: A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. The results showed that the scaffold has great potential in promoting chondrocyte proliferation, maintaining the structural stability of cartilage, and promoting cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Muhammad Rizwan, Alexander E. G. Baker, Molly S. Shoichet
Summary: Designing simple biomaterials to replicate tissue properties is challenging in tissue engineering. Current biomaterials mainly provide macroscopic tunability of scaffold mechanics and do not reflect the dynamic nature of the natural extracellular microenvironment. There are opportunities to investigate cells capable of locally remodeling matrices for more complex applications.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Adrian Lopez-Canosa, Soledad Perez-Amodio, Elisabeth Engel, Oscar Castano
Summary: Traditional in vitro models for testing biomaterial-driven vascularization lack complexity, while cell culture models based on microfluidic technology offer better tissue biomimicry.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Shuntaro Yamada, Mohammed Ahmed Yassin, Thomas Schwarz, Jan Hansmann, Kamal Mustafa
Summary: The study induced osteogenesis in BMSC without chemical stimuli using a custom-designed laminar flow bioreactor. Results showed significant inhibition of cell proliferation, morphological changes, upregulation of osteogenic markers, and increased deposition of collagen type 1 and calcium under perfusion. This highlights the utility of flow bioreactors in bone tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Ting Yao, Yi Zhang, Mengjiao Lv, Guoqing Zang, Soon Seng Ng, Xiaohua Chen
Summary: The 3D cell culture model is essential in studying liver biology and developing clinically relevant products for liver therapies, capturing critical microenvironmental factors for optimal phenotypes of hepatocytes. This model enables a range of preclinical studies that are not feasible in conventional 2D cell models, highlighting its major attributes and components needed for various applications.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Biochemical Research Methods
Nina Compera, Scott Atwell, Johannes Wirth, Christine von Toerne, Stefanie M. Hauck, Matthias Meier
Summary: This study developed a microfluidic chip platform to simulate adipose tissue in vitro and revealed the differences in adipocytes under low/high glucose conditions using proteomics analysis. This in vitro model can be used to investigate the molecular and functional mechanisms of adipose tissue under normal and pathological conditions.
Article
Chemistry, Multidisciplinary
Tom Kamperman, Niels G. A. Willemen, Cindy Kelder, Michelle Koerselman, Malin Becker, Luanda Lins, Castro Johnbosco, Marcel Karperien, Jeroen Leijten
Summary: This study demonstrates that stimuli-responsive cell-adhesive micromaterials can self-assemble with cells into 3D living composite microtissues and provide control over the biomechanical and biochemical environment, influencing cell behavior.
Review
Biochemistry & Molecular Biology
Ola Habanjar, Mona Diab-Assaf, Florence Caldefie-Chezet, Laetitia Delort
Summary: The 3D culture system has become essential in cancer research and tissue engineering for its ability to mimic tumor microenvironment and provide better biocompatibility. Different types of natural supports, synthetic strategies, and methods for generating normal and tumor spheroids are key factors in constructing ideal 3D models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Ruimin Long, Linrong Shi, Peng He, Jumei Tian, Shibin Wang, Jun Zheng
Summary: Using hydrogel as a filling medium to recombine dispersed microencapsulated cells and form an embedded gel-cell microcapsule complex is a novel approach for tissue construction. In this experiment, researchers used sodium alginate, chitosan, and silk fibroin to prepare different materials for the complex, and investigated the tissue-forming ability of the complex under different culture conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Rumeysa Bilginer, Dilce Ozkendir-Inanc, Umit Hakan Yildiz, Ahu Arslan-Yildiz
Summary: The study aimed to create propolis/PVA hybrid scaffold by electrospinning for 3D cell culture. Propolis enhanced biological properties of scaffold for cell attachment and proliferation, while electrospun nanofibers varied in size. The hybrid scaffold showed potential for cell culturing and tissue engineering.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Engineering, Multidisciplinary
Hieu M. Pham, Yuli Zhang, Jose G. Munguia-Lopez, Simon D. Tran
Summary: Saliva production by salivary glands is essential for oral health. This study investigates the use of Egg White Alginate (EWA) as an alternative scaffold for culturing salivary gland cells and shows that EWA can support cell viability and promote larger spheroid formation compared to Matrigel (R).
Article
Chemistry, Multidisciplinary
Manuel Somoza, Ramon Rial, Zhen Liu, Iago F. Llovo, Rui L. Reis, Jesus Mosqueira, Juan M. Ruso
Summary: This study successfully created doped Hydroxyapatite nanoparticles with magnetic properties using microfluidics and Gadolinium as a contrast agent for medical applications. Computational Fluid Dynamics (CFD) was used to ensure the system worked in the laminar regime and nanoparticles diffused uniformly. The biomaterials were characterized using XRD, FE-SEM, EDX, confocal Raman microscopy, and FT-IR, confirming the successful incorporation of Gadolinium. Magnetic characterization confirmed the paramagnetic behavior of the nanoparticles, showing the potential for advanced nanomaterials in theragnostic applications.
Article
Chemistry, Multidisciplinary
Dirk Rommel, Matthias Mork, Sitara Vedaraman, Celine Bastard, Luis P. B. Guerzoni, Yonca Kittel, Rostislav Vinokur, Nikolai Born, Tamas Haraszti, Laura De Laporte
Summary: This work presents a two-component microgel assembly that creates a 3D macroporous construct for cell growth. The reactive microgel rods, produced through microfluidics, assemble and interlink with each other via a ring opening reaction, resulting in macroporous constructs with pores up to several hundreds of micrometers. The functionalization degree of the microgels affects the crosslinking level, as well as the mechanical and biochemical properties that influence cell adhesiveness and growth within the macroporous structure.
Article
Pharmacology & Pharmacy
Yanping Zhang, Alice Le Friec, Menglin Chen
Summary: The study demonstrates the effectiveness of using anisotropic scaffolds along with electrical stimulation to promote elongation and maturation of muscle cells. The alignment and elongation of myotubes were significantly enhanced under electrical stimulation on the anisotropic scaffold, providing a promising platform for skeletal muscle tissue engineering.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Engineering, Biomedical
Sara C. Santos, Catarina A. Custodio, Joao F. Mano
Summary: This study introduces a novel human protein-derived porous scaffold produced from platelet lysates, which serves as a platform for xeno-free 3D cell culture. The results show that the scaffold has tunable morphology and mechanical properties, making it suitable for human cell culture without the need for animal-derived supplements.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Applied
Zejun Sun, Wenjuan Xu, Liutao Guo, Qiang Han, Jianyi Gao, Jiaping Wang, Yanru Feng, Chengrui Li, Qionglin Liang, Hong-bin Sun, Yang Yang
Summary: The synchronous construction of metal phosphate and phosphorus-doped carbon structures is significant for catalyst design and synthesis, with the potential to improve catalytic activity while eliminating the need for excess phosphorus sources.
JOURNAL OF ENERGY CHEMISTRY
(2022)
Article
Biochemical Research Methods
Jiayan Yu, Xue Jiang, Zenghui Lu, Qiang Han, Zhenling Chen, Qionglin Liang
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wei Wei, Qiang Han, Sheng Tian, Yanping Wang, Huiying Zhang, Haibo Wang, Qionglin Liang
Summary: The successful synthesis of a core-shell structure magnetic microsphere Fe3O4-COF for the separation of active ingredients in traditional Chinese medicine has been demonstrated chemically and morphologically using various characterizations. Optimization of extraction conditions resulted in low detection limits and high recoveries, indicating specific adsorption and resistance to matrix interference. This method provides a new direction for the separation and analysis of active ingredients in traditional Chinese medicine.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Ze-Nan Hu, Yongjian Ai, Wenjuan Xu, Xinyue Zhang, Zejun Sun, Liutao Guo, Rongxiu Guo, Yao Wang, Kelong Ding, Hong-bin Sun, Jianshe Hu, Qionglin Liang, Yang Yang
Summary: This study proposes a strategy of Fe-catalyzed cascade formation of heterointerfaces for comprehending the hydrogen evolution reaction (HER). The calculation results show that the reaction on the MoC-Mo2C heterointerface significantly lowers the energy barriers of the rate-determining steps.
Article
Chemistry, Analytical
Zenghui Lu, Xue Jiang, Qi Yi, Jialiang Xiong, Qiang Han, Qionglin Liang
Summary: In this study, a Fe3O4@SiO2@Proteins@Metal-polyphenol network (MPN) was successfully constructed and applied for separating metabolites and proteins. By the self-assembly and disassembly of MPN, the differences of proteins and metabolites between physiological and pathological biospecimens were accurately characterized. Furthermore, proteins were encapsulated and immobilized in the precipitation while endogenous metabolites were dispersed in the supernatant.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Liandi Guan, Wanting Hu, Hongzhi Zuo, Hua Sun, Yongjian Ai, Meng-qi He, Cheng Ma, Mingyu Ding, Qionglin Liang
Summary: A novel probe with a turn-on NIR fluorescent/photoacoustic response to NADPH was successfully used for monitoring intracellular NADPH and dual-modal imaging of tumor-bearing mice. It shows great potential in studying and understanding tumor energy metabolism and treatment process related to NADPH.
CHEMICAL COMMUNICATIONS
(2023)
Review
Biochemical Research Methods
Lei Wu, Yongjian Ai, Ruoxiao Xie, Jialiang Xiong, Yu Wang, Qionglin Liang
Summary: Organoids and organs-on-a-chip are opening new frontiers in the research of intestinal diseases, providing powerful tools due to their self-organization from stem cells and reproduction of functional properties. They also accelerate the mimicking of the intestinal microenvironment and are expected to outperform traditional models in the future. Combined with other strategies, such as genome editing and 3D printing, they contribute to modeling intestinal homeostasis and disease. This article summarizes the current challenges and future trends in intestinal pathophysiological models.
Review
Chemistry, Multidisciplinary
Meng-Qi He, Yongjian Ai, Wanting Hu, Liandi Guan, Mingyu Ding, Qionglin Liang
Summary: The unprecedented advances in metal nanoparticle synthesis have opened up new possibilities for their broad applications. The precise control of metal nanoparticle morphology relies on seed-mediated growth methods, which are sensitive to various influencing factors. The understanding of how each factor precisely regulates the morphology of metal nanoparticles is important for further developments and applications.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yongjian Ai, Meng-Qi He, Hua Sun, Xiaomeng Jia, Lei Wu, Xinyue Zhang, Hong-bin Sun, Qionglin Liang
Summary: High-entropy alloy nanoparticles (HEANPs) are attracting attention for their tunability and potential in bioapplication. However, it is challenging to prepare ultra-small high-entropy alloy nanoparticles (US-HEANPs) due to their instability. In this study, PtPdRuRhIr US-HEANPs were fabricated and used as multifunctional nanoplatforms for tumor treatment. The US-HEANPs exhibited peroxidase-like activity and photothermal conversion effect, which effectively ablated cancer cells and treated tumors. This work provides new insights into HEANPs fabrication and opens up research on high-entropy nanozymes and their biomedical applications.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Meng-Qi He, Yongjian Ai, Wanting Hu, Xiaomeng Jia, Lei Wu, Mingyu Ding, Qionglin Liang
Summary: By using DNA as an etching agent, it is possible to control the morphology and optical properties of plasmonic nanoparticles, while also introducing fluorescence properties. This strategy allows for precise tuning of localized surface plasmon resonance (LSPR) and fluorescence-composited dual-mode signals.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Siyuan Tan, Yongjian Ai, Xinchi Yin, Zhichao Xue, Xiang Fang, Qionglin Liang, Xiaoyun Gong, Xinhua Dai
Summary: Artificial cells are synthetic constructs that mimic natural cells and have potential applications in energy science, environmental treatment, and the study of life's origins. Microfluidics has emerged as a promising approach for the construction of artificial cells due to its precise control, high automation, and excellent uniformity. This review summarizes the latest microfluidic techniques used to construct artificial cells, ranging from simple droplets to sophisticated cell-inspired systems. It discusses the characteristics of different methods and showcases the wide-ranging applications of artificial cells. The review also highlights contemporary obstacles and forthcoming advancements in the field of microfluidic-based artificial cells.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Hua Sun, Huibo Qi, Wanting Hu, Liandi Guan, Jianfeng Xue, Yongjian Ai, Yu Wang, Mingyu Ding, Qionglin Liang
Summary: Vigorous nanomedicine offers significant possibilities for effective therapeutics, and nanovesicles (NVs), including liposomes, exosomes, and cytomembranes, show great potential. However, the complex interactions between NVs and cells, especially the heterogeneous extracellular adsorptions, are difficult to analyze due to transient dynamics. This study tracks single NVs to directly observe extracellular adsorptions and reveals their heterogeneous characteristics. The spatiotemporal tracking provides insights into NVs-cell interactions and inspires nano-investigations in nanomedicine.
Article
Engineering, Environmental
Rongxiu Guo, Xinyue Zhang, Ze-Nan Hu, Dun Niu, Xiaodong Li, Hongbin Sun, Qionglin Liang
Summary: As a typical 3d transition metal, Cu exhibits competitive potential in the catalytic transfer hydrogenation of nitriles due to its low price and appropriate catalytic activity. Unfortunately, the Cu-based catalyst usually requires additional metals to realize good catalytic activity, this makes it difficult to understand the role of Cu. Herein, an amino-modified hollow mesoporous double-shell SiO2 @C (AmHMSiO2 @C) microsphere was applied as a nanoreactor, which achieves the high selective hydrogenation of nitriles over the Cu-only catalyst. The conversion and selectivity to primary amines were 100% and 96% respectively at 60 degrees C using amino borane as the hydrogen source, and the TOF reaches 199.75 h-1 for Cu. The unique structure of the Cu@AmHMSiO2 @C enhances the activity and stability of the Cu active sites. Comparative experiments demonstrate that the synergy between the nanoreactor and Cu nanoparticles enables the desirable catalytic performance toward the hydrogenation of nitriles to primary amines under mild reaction conditions. This work offers a distinct case for constructing an efficient Cu-based catalyst for heterogeneous hydrogenation reactions.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Physical
Wenjuan Xu, Wei Zhang, Zejun Sun, Liutao Guo, Liping Xie, Chengrui Li, Yanru Feng, Qionglin Liang, Yang Yang, Hong-bin Sun
Summary: Urea-assisted electrolytic hydrogen production has lower voltage requirements and shows promise as an alternative for clean energy and pollution reduction. The development of bell-shaped RuFe/Ni5P4 catalysts through dual doping and phosphidation processes has demonstrated high hydrogen production efficiency.
SUSTAINABLE ENERGY & FUELS
(2022)
Review
Pharmacology & Pharmacy
Yongjian Ai, Meng-Qi He, Chengxian Wan, Hua Luo, Hongbo Xin, Yitao Wang, Qionglin Liang
Summary: This review summarizes the recent progress in nanoplatform-based reactive oxygen species scavengers for alleviation of ischemia-reperfusion injury. It provides information on the main sites and mechanisms of ischemia-reperfusion injury, as well as the design, fabrication, and administration principles of nanoplatform-based reactive oxygen species scavengers. The application of these scavengers in the treatment of ischemia-reperfusion injury in various organs is also discussed, along with limitations and potential breakthroughs in developing reactive oxygen species scavengers in this field.
ADVANCED THERAPEUTICS
(2022)
