Article
Microbiology
Diocreciano Matias Bero, Sheila Antonio Nhassengo, Ivanildo Pedro Sousa, Silas Oliveira de Sousa, Raiana Scerni Machado, Amanda Meireles Nunes Dias, Cristiane de Sousa Ferreira, Fernanda Marcicano Burlandy, Nilsa de Deus, Edson Elias da Silva
Summary: Prior to the outbreak of vaccine-derived poliovirus in Africa, Mozambique implemented an environmental surveillance program and found a high circulation of non-polio enteroviruses, with no detection of wild or vaccine-derived poliovirus. Environmental surveillance can effectively aid the public health system in monitoring non-polio enteroviruses in addition to poliovirus.
Article
Immunology
Abdullahi Walla Hamisu, Isobel M. Blake, Gerald Sume, Fiona Braka, Abdullateef Jimoh, Habu Dahiru, Mohammed Bonos, Raymond Dankoli, Ahmed Mamuda Bello, Kabir M. Yusuf, Namadi M. Lawal, Fatimah Ahmed, Zainab Aliyu, Doris John, Theresa E. Nwachukwu, Michael F. Ayeni, Nicksy Gumede-Moeletsi, Philippe Veltsos, Sidhartha Giri, Ira Praharaj, Angeline Metilda, Ananda Bandyopadhyay, Ousmane M. Diop, Nicholas C. Grassly
Summary: This study suggests that measuring sewage properties and estimating catchment population can improve the selection of environmental surveillance sites and increase surveillance sensitivity for poliovirus and other enteroviruses.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Yuri Perepliotchikov, Tomer Ziv-Baran, Musa Hindiyeh, Yossi Manor, Danit Sofer, Jacob Moran-Gilad, Laura Stephens, Ella Mendelson, Merav Weil, Ravit Bassal, Emilia Anis, Shepherd Roee Singer, Ehud Kaliner, Gillian Cooper, Manasi Majumdar, Michal Markovich, Daniela Ram, Itamar Grotto, Ronni Gamzu, Javier Martin, Lester M. Shulman
Summary: Efficient response and monitoring of viral outbreaks can be achieved through rapid quantitative RT-PCR methods to infer the number of infected individuals excreting the virus. These methods allow evidence-based intervention strategies to be implemented quickly, especially in outbreaks with asymptomatic infections and limited testing resources.
Review
Microbiology
Laura Bubba, Kimberley S. M. Benschop, Soile Blomqvist, Erwin Duizer, Javier Martin, Alexander G. Shaw, Jean-Luc Bailly, Lasse D. Rasmussen, Anda Baicus, Thea K. Fischer, Heli Harvala
Summary: Wastewater surveillance (WWS) is a method used for detecting the transmission of pathogens through monitoring viral particles in sewage. It can monitor various pathogens, including those that affect children's health. While culture-based methods are still considered the gold standard, more advanced molecular detection and sequencing methods have been developed.
Article
Virology
Anda Baicus, Carmen Maria Cherciu, Mihaela Lazar
Summary: This study identified the presence of SARS-CoV-2 and enteroviruses in sewage water in Romania in 2020, highlighting the need for the development of rapid and more specific tests for detecting SARS-CoV-2 in wastewater.
Article
Virology
Mary M. Alleman, Angela D. Coulliette-Salmond, Pierre Wilnique, Hanen Belgasmi-Wright, Leanna Sayyad, Kimberly Wong, Edmund Gue, Robert Barrais, Gloria Rey-Benito, Cara C. Burns, Everardo Vega
Summary: Haiti established environmental surveillance for polioviruses, expanding to additional cities over time. While no wild or vaccine-derived polioviruses were isolated, Sabin-like polioviruses were sporadically detected. As a result, some sampling sites were terminated based on factors like enterovirus presence and environmental considerations.
Article
Biotechnology & Applied Microbiology
Kazuhiro Kitakawa, Kouichi Kitamura, Hiromu Yoshida
Summary: Long-term efforts are required to implement surveillance programs for the ongoing COVID-19 pandemic, and they will be required even in the postpandemic era. We adopted the existing polio environmental surveillance (ES) system for SARS-CoV-2 sewage monitoring in Japan as a practical and cost-effective approach. The ES system routinely detects enteroviruses from wastewater and, therefore, can be used for enterovirus monitoring. The present study demonstrates how the existing ES system can be used for monitoring enteroviruses and SARS-CoV-2 in sewage.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Editorial Material
Microbiology
David Aponte-Diaz, Matthew R. Vogt, Craig E. Cameron
Summary: EV-D68 contributes significantly to respiratory and neurological diseases. It exploits cellular autophagic compartments and its viral proteins 2B and 3A have difficulty engaging membranes, leading to a delay in RNA synthesis.
Article
Virology
Youngsil Yoon, Yong-Pyo Lee, Deog-Yong Lee, Hye-Jin Kim, June-Woo Lee, Sangwon Lee, Chun Kang, Wooyoung Choi, Joong Hyun Bin, Young Hoon Kim, Myung-Guk Han, Hae Ji Kang
Summary: This study examined acute flaccid paralysis (AFP) surveillance in Korea and found that a significant number of cases were associated with non-polio enteroviruses (NPEV), with EV-A71 being the most common strain detected. The research suggests that EV-A71 plays a major role in neurological diseases in the region, except for Guillain-Barre syndrome (GBS), acute disseminated encephalomyelitis (ADEM), and meningitis.
Article
Immunology
Stefano Fontana, Gabriele Buttinelli, Stefano Fiore, Concetta Amato, Marco Pataracchia, Majlinda Kota, Jela Acimovic, Mia Blazevic, Mirsada Mulaomerovic, Lubomira Nikolaeva-Glomb, Andreas Mentis, Androniki Voulgari-Kokota, Luljeta Gashi, Pranvera Kacaniku-Gunga, Christopher Barbara, Jackie Melillo, Jelena Protic, Svetlana Filipovic-Vignjevic, Patrick M. O'Connor, Alessandra D'Alberto, Riccardo Orioli, Andrea Siddu, Eugene Saxentoff, Paola Stefanelli
Summary: This study analyzed six years of acute flaccid paralysis (AFP) surveillance data from 2015 to 2020 in 10 countries linked to the WHO Regional Reference Laboratory in Italy. The study also included data on polio vaccine coverage from 2015 to 2019 and enterovirus identification and typing. The findings showed a general underreporting of AFP cases and a high heterogeneity in non-polio enterovirus (NPEV) types. The polio vaccine coverage varied among countries based on available data.
Article
Virology
Meizhong Chen, Yong Zhang, Wei Zhang, Shufen Huang, Shuangli Zhu, Caixia Li, Xue Guo, Hanri Zeng, Ling Fang, Bixia Ke, Hui Li, Hiromu Yoshida, Wenbo Xu, Changwen Ke, Xiaoling Deng, Huanying Zheng
Summary: This study investigated the serotype distribution and epidemiological trends of poliovirus isolated from domestic sewage in Guangzhou City, China from 2009 to 2021. The results indicated that environmental surveillance is an important supplement to acute flaccid paralysis case surveillance for evaluating the effectiveness of vaccine immunization strategies and controlling the circulation of vaccine-derived poliovirus.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Environmental Sciences
Hanen Belgasmi, Stacey Jeffries Miles, Leanna Sayyad, Kimberly Wong, Chelsea Harrington, Nancy Gerloff, Angela D. Coulliette-Salmond, Ratigorn Guntapong, Ratana Tacharoenmuang, Apiradee Isarangkul Na Ayutthaya, Lea Necitas G. Apostol, Ma. Anne-Lesley D. Valencia, Cara C. Burns, Gloria-Rey Benito, Everardo Vega
Summary: Acute flaccid paralysis (AFP) surveillance is important for identifying polio cases and vaccination campaigns. Environmental surveillance (ES) is an important adjunct to AFP surveillance. The CAFe method is an economical, sensitive, and cost-effective method for isolating enteroviruses.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Virology
Andres Lizasoain, Daiana Mir, Gisella Masachessi, Adrian Farias, Nelida Rodriguez-Osorio, Matias Victoria, Silvia Nates, Rodney Colina
Summary: This study in Argentina analyzed the diversity of Human Enteroviruses (EVs) in wastewater samples collected in Cordoba during 2011-2012 and 2017-2018. It found a high genetic similarity between environmental strains of echovirus 30 circulating in 2011-2012 and isolates obtained from patients in different locations suffering from aseptic meningitis. The study also highlighted the valuable role of wastewater-based epidemiology in predicting outbreaks before cases appear in the community.
Article
Microbiology
Amy B. Rosenfeld, Edmund Qian Long Shen, Michaela Melendez, Nischay Mishra, W. Ian Lipkin, Vincent R. Racaniello
Summary: Enteroviruses are common human pathogens that can cause a range of serious illnesses. Despite the genetic and antigenic similarity among nonpoliovirus enteroviruses (NPEVs), cross-reactive antibodies can bind and neutralize multiple NPEVs. However, this cross-reactivity cannot be predicted from phylogenetic analysis.
Article
Virology
Magda Rojas-Bonilla, Angela Coulliette-Salmond, Hanen Belgasmi, Kimberly Wong, Leanna Sayyad, Everardo Vega, Fabian Grimoldi, M. Steven Oberste, Ricardo Ruttimann
Summary: An environmental surveillance study was conducted in parallel to the M5-ABMG clinical trial at five locations in Panama, with no detection of novel OPV2 strains in sewage samples but identification of other viruses. One of the nOPV2 candidates has been granted Emergency Use Listing by the World Health Organization.
Article
Anatomy & Morphology
Dmitry Bagrov, Grigory S. Glukhov, Andrey Moiseenko, Maria G. Karlova, Daniil S. Litvinov, Petr A. Zaitsev, Liubov Kozlovskaya, Anna A. Shishova, Anastasia A. Kovpak, Yury Y. Ivin, Anastasia N. Piniaeva, Alexey S. Oksanich, Viktor P. Volok, Dmitry Osolodkin, Aydar A. Ishmukhametov, Alexey M. Egorov, Konstantin Shaitan, Mikhail P. Kirpichnikov, Olga S. Sokolova
Summary: This study characterized the beta-propiolactone inactivated SARS-CoV-2 virions using TEM and AFM, finding that samples prepared using SEC and IEC retained more spikes on the surface and the S proteins were in the pre-fusion conformation. Analytical TEM showed that the inactivated virions retained nucleic acid, demonstrating their potential as a vaccine candidate.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Virology
Andrei A. Deviatkin, Yulia A. Vakulenko, Mariia A. Dashian, Alexander N. Lukashev
Summary: Rabies is a deadly viral zoonosis that is primarily spread through infected dogs. A systematic analysis of rabies virus in steppe and Arctic-like groups revealed rapid long-distance transmission events, mainly within Eurasia. This human-mediated long-distance transmission poses a significant threat and needs to be addressed.
Article
Microbiology
Olga E. Ivanova, Armen K. Shakaryan, Nadezhda S. Morozova, Yulia A. Vakulenko, Tatyana P. Eremeeva, Liubov I. Kozlovskaya, Olga Y. Baykova, Elena Y. Shustova, Yulia M. Mikhailova, Natalia I. Romanenkova, Nadezhda R. Rozaeva, Natela I. Dzhaparidze, Nadezhda A. Novikova, Vladimir V. Zverev, Lyudmila N. Golitsyna, Alexander N. Lukashev
Summary: Surveillance for acute flaccid paralysis syndrome (AFP) in children under 15 is crucial for the Global Polio Eradication Initiative. The identification of non-polio enteroviruses (NPEV) alongside polioviruses in stool samples of AFP cases has provided valuable insights. Enteroviruses A71 and D68 have been definitively linked to AFP, while the association of Enterovirus Coxsackie A2 (CVA2) with AFP is less understood. This study suggests that CVA2 may be a cause of AFP, highlighting the importance of AFP surveillance for both polio control and the study of uncommon AFP agents.
Article
Genetics & Heredity
E. A. Konorov, A. N. Lukashev, N. Yu Oyun
Summary: The study found that Aedes albopictus mosquitoes from Krasnodar have a higher Wolbachia infection rate than those from Sochi, and all Wolbachia strains identified belong to the wAlbB strain. Previous research has shown that this strain can increase egg hatching rate and fertility of mosquitoes in low temperature conditions. Additionally, the Wolbachia genomes from Sochi and Krasnodar differ in the allele frequency spectrum on certain genes.
RUSSIAN JOURNAL OF GENETICS
(2022)
Article
Virology
Marina Beloukhova, Alexander N. Lukashev, Pavel Y. Volchkov, Andrey A. Zamyatnin, Andrei A. Deviatkin
Summary: Adeno-associated viruses (AAVs) are a useful tool for gene therapy delivery. They are currently classified into AAV A and AAV B species within the Dependoparvovirus genus, and further subdivided into 13 serotypes. However, serotype is not a reliable taxonomic category, and the classification needs improvement.
Article
Infectious Diseases
Olga E. Ivanova, Liubov I. Kozlovskaya, Tatiana P. Eremeeva, Armen K. Shakaryan, Alexander P. Ivanov, Olga Y. Baykova, Alexander Y. Krasota, Elena Y. Shustova, Aida N. Mustafina, Nadezhda S. Morozova, Makhtob S. Bobokhonova, Sergei E. Deshevoi, Aidar A. Ishmukhametov
Summary: The study traced the rapid evolution of a Sabin-like poliovirus in a child through repeated stool sampling, highlighting the importance of acute flaccid paralysis surveillance and high vaccination coverage.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Parasitology
Vyacheslav Yurchenko, Daniil S. Chistyakov, Lyudmila Akhmadishina, Alexander N. Lukashev, Jovana Sadlova, Margarita Strelkova
Summary: In this study, we reviewed historical and recent data on Leishmaniaspp. infection in several countries including Turkmenistan, Uzbekistan, Kazakhstan, Kyrgyzstan, Iran, China, and Mongolia. We focused on a complex of co-existing species and their shared reservoirs and vectors. Additionally, we analyzed the presence of dsRNA viruses in these species and discussed future research directions to understand the susceptibility of different Leishmaniaspp. to viral infection.
Article
Virology
Yulia A. Vakulenko, Artem V. Orlov, Alexander N. Lukashev
Summary: Noroviruses infect a wide range of mammals and are the major cause of gastroenteritis in humans. Recombination events between nonstructural (ORF1) and structural genomic regions (ORF2 and ORF3) were found in all analyzed genogroups of noroviruses, although recombination was most prominent between members of GII. The half-life times of recombinant forms of human GI and GII noroviruses were 10.4 and 8.4-11.3 years, respectively, and there was evidence of many recent recombination events.
Article
Medicine, Research & Experimental
Dmitry Kostyushev, Anastasiya Kostyusheva, Sergey Brezgin, Natalia Ponomareva, Natalia F. Zakirova, Aleksandra Egorshina, Dmitry V. Yanvarev, Ekaterina Bayurova, Anna Sudina, Irina Goptar, Anastasiya Nikiforova, Elena Dunaeva, Tatiana Lisitsa, Ivan Abramov, Anastasiia Frolova, Alexander Lukashev, Ilya Gordeychuk, Andrey A. Zamyatnin Jr, Alexander Ivanov, Vladimir Chulanov
Summary: CRISPR-Cas9 systems show remarkable anti-HBV activity by targeting the hepatitis B virus major genomic form, cccDNA. However, inactivating cccDNA alone is not enough to cure the infection, as HBV replication rebounds due to the de novo formation of cccDNA from its precursor, rcDNA. Depleting rcDNA before delivering CRISPR-Cas9 RNPs can prevent viral rebound and promote resolution of HBV infection. These findings lay the groundwork for developing a virological cure of HBV infection using a single dose of short-lived CRISPR-Cas9 RNPs, with the help of widely used reverse transcriptase inhibitors to block cccDNA replenishment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Pharmacology & Pharmacy
Sergey Brezgin, Anastasiya Kostyusheva, Natalia Ponomareva, Ekaterina Bayurova, Alla Kondrashova, Anastasia Frolova, Olga Slatinskaya, Landysh Fatkhutdinova, Georgy Maksimov, Mikhail Zyuzin, Ilya Gordeychuk, Alexander Lukashev, Sergey Makarov, Alexander Ivanov, Andrey A. Zamyatnin Jr, Vladimir Chulanov, Alessandro Parodi, Dmitry Kostyushev
Summary: Biological nanoparticles, such as extracellular vesicles (EVs) and extracellular vesicle-mimetic nanovesicles (EMNVs), have been extensively studied as drug delivery vehicles in medical applications. This study investigated the properties and drug delivery abilities of EVs and EMNVs, and analyzed their use for delivering the chemotherapeutic drug doxorubicin. EMNVs were found to be more effectively internalized, while EVs showed higher intracellular release of doxorubicin.
Review
Biotechnology & Applied Microbiology
Sergey Brezgin, Alessandro Parodi, Anastasiya Kostyusheva, Natalia Ponomareva, Alexander Lukashev, Darina Sokolova, Vadim S. Pokrovsky, Olga Slatinskaya, Georgy Maksimov, Andrey A. Zamyatnin Jr, Vladimir Chulanov, Dmitry Kostyushev
Summary: Extracellular vesicles (EVs), cell-derived biological nanoparticles, have gained significant interest for drug delivery due to their ideal biocompatibility, safety, ability to cross biological barriers, and surface modification potential. However, the translation of EVs into practical applications has been challenging due to issues in up-scaling, synthesis, and quality control methods. Advances in manufacturing technology now allow EVs to carry various therapeutic cargoes, and new technologies have been introduced to improve EV production, isolation, characterization, and standardization.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Infectious Diseases
Tatiana S. Novozhilova, Daniil S. Chistyakov, Lyudmila V. Akhmadishina, Alexander N. Lukashev, Evgeny S. Gerasimov, Vyacheslav Yurchenko
Summary: The evolution of Leishmania is influenced by clonality, sexual reproduction, and geographic isolation. Leishmania turanica populations in Central Asia can be mixed with other species or monospecific and isolated. Genetic analysis revealed no significant differences in the evolution of mixed and monospecific L. turanica populations. However, there were variations in large-scale genomic rearrangements, with genome translocations being the most prominent example. Additionally, L. turanica exhibited a higher level of chromosomal copy number variation compared to its sister species L. major, suggesting active evolutionary adaptation.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Medicine, Research & Experimental
Dmitry Kostyushev, Sergey Brezgin, Anastasiya Kostyusheva, Natalia Ponomareva, Ekaterina Bayurova, Natalia Zakirova, Alla Kondrashova, Irina Goptar, Anastasiya Nikiforova, Anna Sudina, Yurii Babin, Ilya Gordeychuk, Alexander Lukashev, Andrey A. Zamyatnin Jr, Alexander Ivanov, Vladimir Chulanov
Summary: APOBEC/AID cytidine deaminases play an important role in innate immunity and antiviral defenses. Researchers developed a CRISPR-activation-based approach to induce APOBEC/AID overexpression and control its effects on HBV replication and cellular toxicity. The study highlights the potential for precise control of APOBEC/AID activation as a strategy for suppressing HBV replication without toxicity.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)