期刊
VIRUS RESEARCH
卷 264, 期 -, 页码 32-39出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2019.02.014
关键词
ALV-J; Proteomics; CTHRC1; Viral replication
类别
资金
- China-UK Partnership on Global Food Security: Combating avian tumor diseases for sustainable poultry production [31761133002, BB/R012865/1]
- Natural Science Foundation of China [31672521]
- Shandong Modern Agricultural Technology & Industry System [SDAIT-11-04]
- Fund of Shandong Double Tops Program (2017)
- BBSRC [BBS/E/I/00007032, BB/R012865/1] Funding Source: UKRI
The lifecycle of avian leukosis virus subgroup J (ALV-J), a typical tumorigenic retrovirus, is highly dependent upon host cellular proteins. However, there have been few studies directed at uncovering the host proteins responsible for ALV-J replication, which could provide insights into new strategies for ALV-J prevention and control. Here, we used proteomics to identify the association of differential levels of collagen triple helix-repeat-containing 1 (CTHRC1) and with viral replication. Our results revealed that CTHRC1 was significantly upregulated in ALV-J-infected cells in vitro, and these findings were confirmed in vivo. Additionally, CTHRC1 over-expression facilitated ALV-J replication, whereas CTHRC1 knockdown suppressed this activity. Moreover, we found that ALV-J drove CTHRC1 translocation from the nucleus to the cytosol through interactions with the ALV-J envelope glycoprotein. These results revealed CTHRC1 as a shutting protein is recruited by ALV-J to facilitate viral replication.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据