Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Veterinary Sciences
Robin B. Guevarra, Eun Sol Kim, Jin Ho Cho, Minho Song, Jae Hyoung Cho, Jun Hyung Lee, Hyeri Kim, Sheena Kim, Gi Beom Keum, Chan Ho Lee, Won Tak Cho, Suphot Watthanaphansak, Hyeun Bum Kim
Summary: This study evaluated the effects of probiotics, prebiotics, and their synbiotic combination on gut microbiota in weaned piglets. The results showed that they all improved intestinal health in the piglets by modulating the gut microbiota.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Thotsapol Thomrongsuwannakij, Ruethai Narinthorn, Tanakamol Mahawan, Patrick J. Blackall
Summary: This study compared avian pathogenic Escherichia coli (APEC) isolates from commercial broilers and native chickens in terms of phylogenetic grouping, antimicrobial resistance profiles, and resistance genes. The results showed that APEC isolates from commercial broilers had higher multidrug resistance rates and presence of resistant genes compared to native chicken isolates.
Article
Microbiology
Sien De Koster, Moniek Ringenier, Basil Britto Xavier, Christine Lammens, Dieter De Coninck, Katrien De Bruyne, Klaas Mensaert, Marjolein Kluytmans-van den Bergh, Jan Kluytmans, Jeroen Dewulf, Herman Goossens
Summary: This study characterized and traced ESBL-producing and ciprofloxacin-resistant Escherichia coli in Belgian food-producing animals. Multiple sequence types, resistance genes, and virulence profiles were detected. Transmission pathways within and between farms were identified, highlighting the importance of plasmid transmission in the spread of resistance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Han Wang, Fang Yang, Zhe-wen Song, Hao-tian Shao, Dong-ying Bai, Yan-bo Ma, Tao Kong, Fan Yang
Summary: This study found that the challenge of Escherichia coli lipopolysaccharide (LPS) affects the pharmacokinetics of danofloxacin in broilers, leading to decreased AUC, Cmax, and t(1/2 lambda z) parameters, and increased Tmax and lambda z parameters.
Article
Multidisciplinary Sciences
Jude Fonbah Leinyuy, Innocent Mbulli Ali, Karimo Ousenu, Christopher B. Tume
Summary: This study analyzed 200 Enterobacteriaceae isolates resistant and susceptible to ESBL, quinolones, and aminoglycosides. It found a high carriage of resistance genes, but the carriage of resistance genes may not always be correlated with phenotypic expression.
Article
Multidisciplinary Sciences
Ahmed R. Elbestawy, Hany F. Ellakany, Hatem S. Abd El-Hamid, Madiha S. Ibrahim, Ahmed R. Gado, Nagwa S. Mustafa, Ihab M. Moussa, Khalid S. Al-Maary, Dalia S. Al-Sarar, Hanan O. Alshammari, Turki M. Dawoud, Hassan A. Hemeg, Hussein M. Galal
Summary: This study evaluated the cross-protection ability of live attenuated E. coli vaccine against heterologous APEC serotypes, showing significant protection but subtle differences in clinical signs, post-mortem lesions, mortality rates, E. coli re-isolation, histopathological examination, final body weight, and feed conversion ratio.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Francois Bertaux, Sebastian Sosa-Carrillo, Viktoriia Gross, Achille Fraisse, Chetan Aditya, Mariela Furstenheim, Gregory Batt
Summary: Small-scale, low-cost bioreactors are gaining popularity in quantitative systems and synthetic biology. In this study, the authors present ReacSight, a strategy that enhances bioreactor arrays by connecting them with sensitive measurement devices using low-cost pipetting robots. They demonstrate the capabilities of ReacSight in yeast, including real-time optogenetic control of gene expression, exploration of nutrient scarcity effects on fitness and cellular stress, and dynamic control of a two-strain consortium's composition.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Dan Song, Aike Li, Yongwei Wang, Ge Song, Junlin Cheng, Li Wang, Kuanbo Liu, Yuna Min, Weiwei Wang
Summary: The overuse of in-feed antibiotics can lead to antibiotic resistance and drug residues in poultry products. This study investigated the effects of a synbiotic, composed of microencapsulated Lactobacillus plantarum and fructooligosaccharide, on the growth, immune and antioxidant parameters, and digestibility of calcium and phosphorus in broilers. The results showed that the synbiotic had positive effects on the measured parameters, suggesting its potential as a substitute for antibiotics.
Article
Biochemistry & Molecular Biology
Eman Hassanen, Eman A. Morsy, Ahmed M. Hussien, Khaled Y. Farroh, Merhan E. Ali
Summary: The study investigated the bactericidal effect of copper, silver, and chitosan nanoparticles against E. coli infection in broilers, and found that Ch-Ag NPs not only had the best antibacterial effects, but also acted as a growth promoter in broilers without leaving any residues in edible organs.
BIOSCIENCE REPORTS
(2021)
Article
Chemistry, Applied
Ahmed Elbermawi, Mohamed Samir Darwish, Asmaa A. El-Awady, Ahmed A. Zaki, Longxin Qiu, Reham M. Samra
Summary: This study evaluated the prebiotic potential and enzyme inhibition of Rumex vesicarius. The roots of R. vesicarius were found to contain compounds that can promote the growth of Escherichia coli and inhibit enzyme activities. These findings suggest that R. vesicarius may serve as a potential prebiotic and have implications for the treatment of diabetes, weight control, and blood pressure regulation.
Article
Biology
Samah Eid, Hala M. N. Tolba, Rehab Hamed, Nayera M. Al-Atfeehy
Summary: Avian pathogenic Escherichia coli (APEC) is a severe issue to both poultry business and public health. This study focused on the isolation of E. coli from diseased broiler chickens and investigated their antimicrobial resistance and biofilm forming capacity. Phages were also studied to determine their presence in E. coli isolates and their ability to inhibit and dissolve biofilms. The results showed that phage therapy could be an attractive option for preventing and controlling multidrug resistant colibacillosis in broilers.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Menghua Wang, Katherine S. Ryan
Summary: In this study, two new l-Alanosine-producing strains were discovered through genome mining. These strains lack the aspartate-nitrosuccinate pathway genes found in the original producer and use a unique set of nitrate-nitrite reductases to reduce nitrate instead. The repurposing of enzymes from the nitrogen cycle for specialized metabolite biosynthesis and the potential for the discovery of new nitric-oxide-derived natural products are demonstrated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Sheng-Yan Chen, Yan Zhang, Renjie Li, Baojun Wang, Bang-Ce Ye
Summary: This study improves the performance of whole-cell biosensors for arsenic contamination by optimizing the promoter design. The arsenic-responsive system from Escherichia coli is used as the sensing element, and a genetic circuit is constructed to characterize the refactored promoters. A novel promoter with maximal repression efficiency and optimal fold change is discovered, and high sensitivity fluorescence and colorimetric sensors are developed for quantifying arsenic levels in groundwater.
ANALYTICAL CHEMISTRY
(2022)
Article
Green & Sustainable Science & Technology
Anna Xexaki, Dimitrios K. Papadopoulos, Maria V. Alvanou, Ioannis A. Giantsis, Konstantinos V. Papageorgiou, Georgios A. Delis, Vangelis Economou, Spyridon K. Kritas, Evangelia N. Sossidou, Evanthia Petridou
Summary: This study aimed to examine the presence of antibiotic-resistant Escherichia coli strains in poultry farms. The results showed that the highest resistance rates were observed for sulfamethoxazole (81.1%), nalidixic acid (73.6%), tetracycline (70.8%), and streptomycin (70.8%). The isolates exhibited lower resistance levels to third generation cephalosporins, with ceftazidime (2.8%), ceftriaxone (3.7%), cefoxitin (4.7%), and cefotaxime (4.7%). Phenotypic tests revealed that 13.6% and 10.2% of the isolates produced ESBL, while 2.7% and 1% produced AmpC β-lactamase, in broiler and laying hens, respectively. The prevalence of the mcr-1 gene was 22.7%, detected only in broiler isolates. Based on these findings, E. coli antibiotic resistance represents a critical control point in poultry production, which may have implications for public health.
Article
Agriculture, Dairy & Animal Science
Mahmoud G. El Sebaei, Nagah Arafat, Reham A. El-Shafei, Mohamed A. El-Adl, Amany Farag, Abeer E. Aziza, Abdelfattah H. Eladl
Summary: This study demonstrated that dietary imbalance caused by high levels of calcium or protein in chickens is harmful, leading to post-mortem lesions, alterations in biochemical and redox status, histopathological changes, and upregulation of inflammatory cytokines. The birds fed with a high-Ca diet showed the most significant alterations in most endpoints.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2021)
Article
Parasitology
Ryo Nakao, Kohei Shinjo, Tomoki Sakiyama, Shohei Ogata, Kodai Kusakisako, Gohta Kinoshita, Doaa Naguib, Elisha Chatanga, Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Keita Matsuno, Takuya Ito, Nariaki Nonaka, Mariko Sashika, Toshio Tsubota, Michito Shimozuru
Summary: The research reported the discovery of a partially engorged Amblyomma testudinarium tick on a wild brown bear in Hokkaido, Japan. Genetic characterization of the specimen through complete mitochondrial genome sequencing revealed clustering with A. testudinarium from Nara, Japan, providing important insights on the distribution and host range of this tick species. This study represents the northernmost detection point of the tropical tick A. testudinarium.
PARASITOLOGY INTERNATIONAL
(2021)
Article
Microbiology
Wessam Mohamed Ahmed Mohamed, Alsagher O. Ali, Hassan Y. A. H. Mahmoud, Mosaab A. Omar, Elisha Chatanga, Bashir Salim, Doaa Naguib, Jason L. Anders, Nariaki Nonaka, Mohamed Abdallah Mohamed Moustafa, Ryo Nakao
Summary: The study found that the bacteria and eukaryotic microorganisms circulating in the blood of dromedary camels are dominated by Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Fungi, Charophyta, and Apicomplexa. The genera Sediminibacterium, Hydrotalea, Bradyrhizobium, Anaplasma, Theileria, Sarcocystis, Hoplorhynchus, and Stylocephalus were found to be the most abundant. The metagenomic approach successfully detected several pathogens including Anaplasma sp., Theileria ovis, and Trypanosoma evansi type A.
Article
Microbiology
Doaa Naguib, Dawn M. Roellig, Nagah Arafat, Lihua Xiao
Summary: This study investigated Cryptosporidium spp. in rabbits from different farms in Egypt, finding an overall detection rate of approximately 11.9%. All 28 samples tested positive for Cryptosporidium cuniculus, with 16 samples successfully subtyped into two subtypes. These findings suggest that Cryptosporidium spp. in rabbits from Egypt have zoonotic potential.
Article
Veterinary Sciences
Ehab Kotb Elmahallawy, Alaa Fehaid, Dina M. M. EL-shewehy, Amany M. Ramez, Abdulsalam A. M. Alkhaldi, Rehab Mady, Nasr Elsayed Nasr, Nagah Arafat, Eman A. A. Hassanen, Khalaf F. Alsharif, Walied Abdo
Summary: Avian coccidiosis is a major parasitic disease in the poultry industry, and its treatment mainly relies on the use of anticoccidial drugs. However, a new study has found that S-Methylcysteine (SMC), a compound derived from garlic, shows promising activity in treating avian coccidiosis. SMC exhibits potent anticoccidial effects, as well as antioxidant and anti-inflammatory properties.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Parasitology
Doaa Naguib, Dawn M. Roellig, Nagah Arafat, Lihua Xiao
Summary: This study conducted a molecular epidemiological study of E. bieneusi in child care centers in Egypt. The results showed that the infection rate of E. bieneusi was 4.6% among children in these centers, and five genotypes were identified. These data provide important information on the epidemiology and genetic diversity of E. bieneusi in children in Egypt.
PARASITOLOGY RESEARCH
(2022)
Article
Evolutionary Biology
Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, May June Thu, Keita Kakisaka, Elisha Chatanga, Shohei Ogata, Naoki Hayashi, Yurie Taya, Yuma Ohari, Doaa Naguib, Yongjin Qiu, Keita Matsuno, Saw Bawm, Lat Lat Htun, Stephen C. Barker, Ken Katakura, Kimihito Ito, Nariaki Nonaka, Ryo Nakao
Summary: This study analyzed the population structure, evolutionary dynamics, and phylogeographic relationship of Amblyomma testudinarium in different geographical regions in Japan, suggesting a distinct population structure of A. testudinarium in Japan and potentially several cryptic Amblyomma spp. closely related to A. testudinarium in Asia.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Tianpeng Wang, Yaqiong Guo, Dawn M. Roellig, Na Li, Monica Santin, Jason Lombard, Martin Kvac, Doaa Naguib, Ziding Zhang, Yaoyu Feng, Lihua Xiao
Summary: Genetic recombination between sympatric ancestral populations leads to the emergence of divergent subpopulations of the zoonotic parasite Cryptosporidium parvum with modified host ranges. This study reveals the ancestral origins of C. parvum and suggests that pathogen import through modern animal farming promotes the emergence of subpopulations with modified host preference.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Genetics & Heredity
Reham M. El-Tarabili, Elsayyad M. M. Ahmed, Nada K. K. Alharbi, Maha A. A. Alharbi, Ahlam H. H. AlRokban, Doaa Naguib, Sadeq K. K. Alhag, Tamer Mohamed El Feky, Ahmed Ezzat Ahmed, Ahmed E. E. Mahmoud
Summary: This study investigated the prevalence, antibiogram, virulence, ESBLs, and non-beta-lactam encoding genes of Proteus species isolated from infected dogs. The results showed high multidrug resistance in Proteus species, which is the first report in dogs in Egypt.
FRONTIERS IN GENETICS
(2022)
Article
Microbiology
Doaa Naguib, Nausicaa Gantois, Jeremy Desramaut, Nagah Arafat, Gael Even, Gabriela Certad, Magali Chabe, Eric Viscogliosi
Summary: Despite the widespread distribution of Blastocystis sp., limited epidemiological investigations have been conducted on animal groups that come in contact with humans. This study aimed to investigate the prevalence and subtype distribution of Blastocystis sp. in animals in Egypt, and found that chickens and cattle had the highest infection rates. Cats and dogs had lower infection rates, suggesting that they are not natural hosts of Blastocystis sp.
Article
Microbiology
Linh Do Ngoc Nguyen, Nausicaa Gantois, Trung Thanh Hoang, Bong Thi Do, Jeremy Desramaut, Doaa Naguib, Tuan Ngoc Tran, Anh Duc Truong, Gael Even, Gabriela Certad, Magali Chabe, Eric Viscogliosi
Summary: Despite being the most common enteric protozoan worldwide, there is still a lack of knowledge regarding the prevalence and subtype distribution of Blastocystis sp. in certain areas, particularly in Southeast Asian countries like Vietnam. This study conducted the first molecular epidemiological survey in Vietnam, which revealed a prevalence rate of 34.5% and identified several subtypes, including ST8, ST10, and ST14, not previously reported in the Southeast Asian population. The study also highlighted a high risk of zoonotic transmission, mainly from poultry and livestock.
Article
Microbiology
Doaa Naguib, Nausicaa Gantois, Jeremy Desramaut, Nagah Arafat, Mohamed Mandour, Asmaa Kamal Kamal Abdelmaogood, Ashraf Fawzy Mosa, Constance Denoyelle, Gael Even, Gabriela Certad, Magali Chabe, Eric Viscogliosi
Summary: Blastocystis sp. is actively circulating in Northern Egypt with significant variations in prevalence and subtype distribution among different governorates. Age, sex, digestive symptoms, and contact with animals were not identified as significant risk factors for the occurrence of the parasite. ST3 was found to be the most prevalent subtype and a widespread inter-human transmission was suggested.
Article
Biochemistry & Molecular Biology
Mohamed Abdallah Mohamed Moustafa, Wessam Mohamed Ahmed Mohamed, Alice C. C. Lau, Elisha Chatanga, Yongjin Qiu, Naoki Hayashi, Doaa Naguib, Kozue Sato, Ai Takano, Keita Matsuno, Nariaki Nonaka, DeMar Taylor, Hiroki Kawabata, Ryo Nakao
Summary: Research on vector-associated microbiomes has found that different tick species and life stages have distinct microbial community structures. The dominant symbiont in these ticks is Coxiella, but other symbionts and potential pathogens were also identified. The formation of microbiomes is related to the balance between environmental bacterial exposure and endosymbiont ecology.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)