Review
Cell Biology
Fangfang Yang, Guoyun Xuan, Yixin Chen, Lichao Cao, Min Zhao, Chen Wang, Erfei Chen
Summary: Colorectal cancer is a common type of cancer and a leading cause of mortality worldwide. MicroRNAs play important roles in the development and progression of colorectal cancer, serving as clinical biomarkers and therapeutic targets. However, the complex network of miRNA/target regulation in colorectal cancer remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Soudeh Ghafouri-Fard, Bashdar Mahmud Hussen, Elham Badrlou, Atefe Abak, Mohammad Taheri
Summary: miRNAs play a significant role in CRC by influencing the expression and activity of CRC-related pathways and interacting with long non-coding RNAs. Levels of miRNAs can be used to distinguish CRC patients from healthy controls. Despite evaluation of therapeutic application of miRNAs in CRC in animal models, it has not been verified in clinical settings.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Oncology
Yufei Tang, Shaoqi Zong, Hailun Zeng, Xiaofeng Ruan, Liting Yao, Susu Han, Fenggang Hou
Summary: MiRNAs are small noncoding RNA molecules consisting of only 20-22 nucleotides, involved in gene silencing and translation suppression by targeting and binding to mRNA. They play a crucial role in maintaining a normal physiological environment by influencing various cellular pathways, including those related to cancer development. MiRNAs are actively studied for their bidirectional regulation of angiogenesis in colorectal cancer, with implications for developing new therapeutic strategies.
CANCER CELL INTERNATIONAL
(2021)
Article
Urology & Nephrology
Mehmet Kaba, Necip Pirincci, Murat Demir, Sultan Kaba, Serdar Oztuzcu, Samed Verep
Summary: By evaluating the expression of miRNAs in plasma samples, it was found that the levels of miRNAs were significantly elevated in patients with non-muscle invasive bladder cancer compared to the control group. This suggests that miRNA levels could serve as useful plasma biomarkers for bladder cancer.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2023)
Article
Gastroenterology & Hepatology
Kai Li, Yusheng Lin, Yu Zhou, Xiao Xiong, Lu Wang, Junkuo Li, Fuyou Zhou, Yi Guo, Shaobin Chen, Yuping Chen, Hui Tang, Xiaofu Qiu, Songwang Cai, Dianzheng Zhang, Edwin Bremer, Sai-Ching Jim Yeung, Hao Zhang
Summary: This study aims to establish a biomarker for early detection and risk stratification of esophageal squamous cell carcinoma (ESCC) using microRNA (miRNA) derived from salivary extracellular vesicles and particles (EVPs). The study identifies a 6-miRNA signature that accurately distinguishes patients with early-stage ESCC from control subjects, and successfully validates its effectiveness.
Review
Cell Biology
Jossimar Coronel-Hernandez, Izamary Delgado-Waldo, David Cantu de Leon, Cesar Lopez-Camarillo, Nadia Jacobo-Herrera, Rosalio Ramos-Payan, Carlos Perez-Plasencia
Summary: Hypoxia in cancer is a well-studied phenomenon caused by tumor growth itself. Hypoxic cells are rescued through the accumulation of HIF-1 alpha in the cytoplasm, which regulates different mechanisms through the genes it induces. A group of microRNAs called hypoxaMIRs, regulated by HIF, have been identified.
Article
Cell Biology
Neda Yaghoubi, Farnaz Zahedi Avval, Majid Khazaei, Seyed Hamid Aghaee-Bakhtiari
Summary: miRNAs, as highly sensitive and specific markers, play important roles in the diagnosis, prognosis, and treatment of colorectal cancer. They can serve as quantifiable and effective molecular tools, offering potential new strategies for early cancer detection and treatment.
CELLULAR SIGNALLING
(2021)
Article
Genetics & Heredity
Antonio A. C. Modesto, Milene R. de Moraes, Cristina M. D. Valente, Marta S. C. R. Costa, Diana F. da V. B. Leal, Esdras E. B. Pereira, Marianne R. Fernandes, Jhully A. dos S. Pinheiro, Karla B. C. C. Pantoja, Fabiano C. Moreira, Rommel M. R. Burbano, Paulo P. de Assumpcao, Ney P. C. dos Santos, Sidney E. B. dos Santos
Summary: Gastric cancer is a complex and aggressive disease with a high prevalence and mortality worldwide. INDELs in miRNA and its target sites have been found to play an important role in the development of cancer. This study identified several variants (hsa-miR-4463, hsa-miR-3945, hsa-miR-548H_4, hsa-miR-920, and hsa-miR-3652) associated with gastric cancer susceptibility and clinical features.
Review
Oncology
Hussein Al-Akhrass, Niki Christou
Summary: MiRNAs are increasingly important in the diagnosis, prognosis, and treatment decisions for colorectal cancers, with the need to identify the most sensitive and specific miRNAs through multicentric prospective studies for different goals. MiRNAs are becoming a key part of the healthcare management armamentarium for colorectal cancers due to their diversity and feasibility of use.
Review
Cell Biology
Soudeh Ghafouri-Fard, Mohammadreza Hajiesmaeili, Hamed Shoorei, Zahra Bahroudi, Mohammad Taheri, Guive Sharifi
Summary: Stem cells and cancer stem cells possess the abilities of self-renewal and differentiation, with molecular pathways like Wnt, Notch, and Hedgehog influencing their functions. miRNAs and lncRNAs have been found to regulate the functions of CSCs, particularly in breast cancer and hepatocellular carcinoma.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Yuxin Lin, Zhijun Miao, Xuefeng Zhang, Xuedong Wei, Jianquan Hou, Yuhua Huang, Bairong Shen
Summary: Translational informatics provides new perspectives for PCa biomarker prioritization and carcinogenic survey, with nine miRNAs identified as key players for PCa management. Most of these miRNAs achieved high AUC values in differentiating prostate samples, with seven of them previously reported as PCa biomarkers. The identified miRNAs-mRNAs were significantly enriched in pathways related to Wnt signaling, prostate cancer, and microRNAs in cancer, demonstrating their functional role in PCa genesis.
FRONTIERS IN GENETICS
(2021)
Article
Biology
Yanping Jiang, Kulsoom Ghias, Sanjeev Gupta, Ananya Gupta
Summary: miRNAs play a crucial role in cancer treatment as biomarkers for tissue damage and health-related fitness. These biomarkers can be correlated with circulating miRNA levels, providing a way to assess progress in rehabilitation.
Article
Oncology
Yukihiro Yoshikawa, Mitsuko Fukunaga, Junichi Takahashi, Dai Shimizu, Takaaki Masuda, Tsunekazu Mizushima, Kazutaka Yamada, Masaki Mori, Hidetoshi Eguchi, Yuichiro Doki, Takahiro Ochiya, Koshi Mimori
Summary: This study identified the ideal combination of miRNAs for predicting colorectal cancer recurrence. These miRNAs showed better predictive ability compared to conventional tumor markers.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Review
Medicine, General & Internal
Daniel Sur, Shailesh Advani, Dejana Braithwaite
Summary: The findings of this study suggest that miRNA panels, especially those derived from serum, can serve as powerful and promising biomarkers for early CRC screening. These panels have high sensitivity and specificity, and show excellent diagnostic accuracy for CRC.
FRONTIERS IN MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Ganna Aleshcheva, Christian Baumeier, Dominik Harms, C. -Thomas Bock, Felicitas Escher, Heinz-Peter Schultheiss
Summary: This study assessed miRNAs in virus-negative inflammatory dilated cardiomyopathy (DCMi) patients and found that miR-1, miR-23, miR-142-5p, miR-155, miR-193, and miR-195 were down-regulated in DCMi patients. These miRNAs effectively differentiated inflammation unlinked to viruses (DCMi) from other participant groups, with a specificity exceeding 86%.
Article
Immunology
Evangelos Andreakos, Laurent Abel, Donald C. Vinh, Elzbieta Kaja, Beth A. Drolet, Qian Zhang, Cliona O'Farrelly, Giuseppe Novelli, Carlos Rodriguez-Gallego, Filomeen Haerynck, Carolina Prando, Aurora Pujol, Helen C. Su, Jean-Laurent Casanova, Andras N. Spaan, Paul Bastard, Catherine M. Biggs, Benedetta Bigio, Bertrand Boisson, Alexandre Bolze, Anastasiia Bondarenko, Petter Brodin, Samya Chakravorty, John Christodoulou, Aurelie Cobat, Antonio Condino-Neto, Stefan N. Constantinescu, Hagit Baris Feldman, Jacques Fellay, Rabih Halwani, Emmanuelle Jouanguy, Yu-Lung Lau, Isabelle Meyts, Trine H. Mogensen, Satoshi Okada, Keisuke Okamoto, Tayfun Ozcelik, Qiang Pan-Hammarstrom, Anna M. Planas, Anne Puel, Lluis Quintana-Murci, Laurent Renia, Igor Resnick, Anna Sediva, Anna Shcherbina, Ondrej Slaby, Ivan Tancevski, Stuart E. Turvey, K. M. Furkan Uddin, Diederik van de Beek, Mayana Zatz, Pawel Zawadzki, Shen-Ying Zhang
Summary: SARS-CoV-2 infections can vary greatly between individuals, with some being asymptomatic while others experience life-threatening disease. While 20% of critical COVID-19 cases are due to inborn errors or autoantibodies, the genetic and immunological factors for resistance to infection itself remain unknown. Studies have shown that autosomal recessive deficiencies in certain genes or enzymes can confer resistance to diseases, suggesting a potential strategy for identifying individuals naturally resistant to SARS-CoV-2 infection.
Article
Pharmacology & Pharmacy
Martin Krkoska, Jana Nekvindova, Katerina Nevedelova, Veronika Zubanova, Lenka Radova, Jan Vondracek, Jarmila Herudkova, Ondrej Slaby, Igor Kiss, Lucia Bohovicova, Pavel Fabian, Zuzana Tylichova, Zdenek Kala, Petr Kysela, Lenka Ostrizkova, Vladimir Palicka, Alena Hyrslova Vaculova
Summary: The study revealed a potential link between miRNAs and downregulation of drug-metabolizing CYPs in HCC tumors, with miR-29c and miR-653 acting as efficient suppressors of CYP1A2 or AHR expression. This highlights a novel role of miR-653 and miR-29c in regulating CYPs involved in crucial biotransformation processes in liver, often deregulated during liver cancer progression.
PHARMACOLOGICAL REPORTS
(2022)
Article
Surgery
Tomas Novotny, Robert Staffa, Josef Tomandl, Tomas Krivka, Ondrej Slaby, Lubos Kubicek, Ernest Biros, Marie Tomandlova, Robert Vlachovsky, Jan Sponiar, Lenka Radova, Anna Konieczna
Summary: This study aimed to evaluate the value of L-lactate as a marker for early detection of postoperative intestinal ischemia. The results showed that measuring serum L-lactate levels at proper timepoints might help in the early diagnosis of postoperative intestinal ischemia after aortic surgery.
INTERNATIONAL JOURNAL OF SURGERY
(2022)
Article
Oncology
Tomas Hrbac, Alena Kopkova, Frantisek Siegl, Marek Vecera, Michaela Ruckova, Tomas Kazda, Radim Jancalek, Michal Hendrych, Marketa Hermanova, Vaclav Vybihal, Pavel Fadrus, Martin Smrcka, Filip Sokol, Vaclav Kubes, Radim Lipina, Ondrej Slaby, Leos Kren, Jiri Sana
Summary: HLA-E and HLA-F play an important role in GBM biology and could be used as diagnostic and prognostic biomarkers.
CANCER GENOMICS & PROTEOMICS
(2022)
Article
Medical Laboratory Technology
Julia Bohosova, Marek Kasik, Adela Kubickova, Karolina Trachtova, Michal Stanik, Alexandr Poprach, Ondrej Slaby
Summary: This study used next-generation sequencing to comprehensively analyze the expression profiles of lncRNAs in renal cell carcinoma patients, and identified several dysregulated lncRNAs associated with the disease. Among them, PVT1 was found to be significantly dysregulated in renal cell carcinoma and further experiments confirmed its effect on cell viability and migration. These results suggest that PVT1 not only has diagnostic potential, but also may serve as a potential target for the treatment of renal cell carcinoma.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Health Care Sciences & Services
Cecilia Pop-Bica, Cristina Alexandra Ciocan, Cornelia Braicu, Antonia Harangus, Marioara Simon, Andreea Nutu, Laura Ancuta Pop, Ondrej Slaby, Atanas G. Atanasov, Radu Pirlog, Nadim Al Hajjar, Ioana Berindan-Neagoe
Summary: This study investigated the genetic profile of lung cancer using TCGA datasets and NGS experiments, revealing differences in mutational landscape among histotypes. The findings suggest that certain genetic alterations may be correlated with patient survival rates.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Oncology
Jaroslav Juracek, Marie Madrzyk, Michal Stanik, Ondrej Slaby
Summary: This review discusses the potential application of urinary miRNA in the diagnosis of prostate cancer and the technical aspects affecting its analysis. Despite numerous studies on this topic, there is little agreement on the obtained data. Therefore, further independent validation and the use of rigorous methodologies are needed to optimize the clinical application of urinary miRNA.
Review
Oncology
Frantisek Siegl, Marek Vecera, Ivana Roskova, Martin Smrcka, Radim Jancalek, Tomas Kazda, Ondrej Slaby, Jiri Sana
Summary: The importance of microRNAs in brain metastasis, as well as their potential clinical implications, are summarized in this review. MicroRNAs are small non-coding RNAs that play a crucial role in regulating gene expression and cellular processes. They have been implicated in various cancers, including brain metastases, and show promise as diagnostic molecules and therapeutic targets.
Review
Medicine, General & Internal
Jitka Vaculova, Radek Kroupa, Zdenek Kala, Jiri Dolina, Tomas Grolich, Jakub Vlazny, David Said, Lydie Izakovicova Holla, Petra Borilova Linhartova, Vladimir Prochazka, Marek Joukal, Petr Jabandziev, Ondrej Slaby, Lumir Kunovsky
Summary: Confocal laser endomicroscopy (CLE) is a real-time microscopic imaging technique used mainly in gastroenterology, particularly in the diagnosis of Barrett's esophagus and esophageal adenocarcinoma. Although many studies have shown that CLE increases diagnostic accuracy in detecting neoplastic transformation of BE, it is still not widely used as a standard diagnostic tool in BE surveillance due to a lack of scientific evidence.
Article
Medical Laboratory Technology
Marie Tomandlova, Tomas Novotny, Robert Staffa, Jindra Smutna, Tomas Krivka, Peter Kruzliak, Ondrej Slaby, Lubos Kubicek, Robert Vlachovsky, Lenka Radova, Josef Tomandl
Summary: This study investigates the perioperative kinetics of D-lactate and ischemia-modified albumin (IMA) and their predictive value in early diagnosis of acute intestinal ischemia. The results suggest that serum levels of IMA and D-lactate can serve as influential predictive markers for postoperative intestinal ischemia, especially after 3 hours from complete declamping of vascular reconstruction.
CLINICAL BIOCHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Jaroslav Juracek, Marie Madrzyk, Michal Stanik, Michaela Ruckova, Karolina Trachtova, Hana Malcikova, Eva Lzicarova, Dominik A. Barth, Martin Pichler, Ondrej Slaby
Summary: This study identified a miRNA expression pattern associated with disease aggressiveness in prostate cancer patients and established a prognostic score combining miRNA levels and serum PSA level that can distinguish between benign and aggressive prostate cancer.
Article
Oncology
Ivana Roskova, Marek Vecera, Lenka Radova, Karolina Trachtova, Frantisek Siegl, Marketa Hermanova, Michal Hendrych, Leos Kren, Vaclav Vybihal, Hana Valekova, Petra Kasparova, Ivana Kolouskova, Tomas Kazda, Ondrej Slaby, Radim Jancalek, Jiri Sana, Martin Smrcka
Summary: This study aimed to discover novel biomarkers for the origin of brain metastases (BMs) and develop a diagnostic classifier. The study used miRNA sequencing and RT-qPCR validation, and identified 6 miRNAs as diagnostic markers for BMs.
CANCER GENOMICS & PROTEOMICS
(2023)
Article
Genetics & Heredity
Katerina Slaba, Marta Jezova, Petra Pokorna, Hana Palova, Jana Tuckova, Jan Papez, Dagmar Prochazkova, Petr Jabandziev, Ondrej Slaby
Summary: Cardiac-urogenital syndrome is a rare syndrome associated with pathogenic variants in the MYRF gene, leading to loss of protein function. It is characterized by complex congenital cardiac defects, pulmonary hypoplasia, diaphragmatic hernia, and urogenital malformations. We report a case of two siblings with extensive developmental defects, who were found to have a novel splicing pathogenic variant in the MYRF gene. The presence of the identical de novo event in siblings suggests gonadal mosaicism, which mimics recessive inheritance. This is the first published case indicating gonadal mosaicism in familial cardiac-urogenital syndrome.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Silvia Toporcerova, Ivana Spakova, Katarina Soltys, Zuzana Klepcova, Marek Kl'oc, Julia Bohosova, Karolina Trachtova, Lucia Peterova, Helena Mickova, Peter Urdzik, Maria Marekova, Ondrej Slaby, Miroslava Rabajdova
Summary: The increased interest in assisted reproduction through in vitro fertilization (IVF) has created a need for biomarkers that can highly predict the success of pregnancy. Researchers have found that analyzing short non-coding RNA (sncRNA) molecules secreted during the IVF process using artificial intelligence can enhance our understanding of communication between embryonic cells and the endometrium, leading to increased success rates.
Article
Medicine, General & Internal
Michal Hendrych, Peter Solar, Marketa Hermanova, Ondrej Slaby, Hana Valekova, Marek Vecera, Alena Kopkova, Zdenek Mackerle, Tomas Kazda, Petr Pospisil, Radek Lakomy, Jan Chrastina, Jiri Sana, Radim Jancalek
Summary: This case report describes a 43-year-old female patient with frontal glioblastoma who developed intraspinal metastasis five months after surgery. Next-generation sequencing analysis revealed a higher number of single nucleotide variants in the metastatic sample compared to the primary tumor. Mutations in NF1, NOTCH3, and ARID1A were identified and may contribute to the invasiveness and metastatic potential of this particular glioblastoma case.
