Article
Biochemistry & Molecular Biology
Yong Shi, Anne Berking, Timo Baade, Kyle R. Legate, Reinhard Fassler, Christof R. Hauck
Summary: Staphylococcus aureus invades host cells mainly through an integrin-dependent manner, with phosphatidylinositol-4,5-bisphosphate (PI-4,5-P-2) playing a significant role in regulating integrin-associated proteins. Local synthesis of PI-4,5-P-2 by a focal adhesion-associated lipid kinase is crucial for integrin-mediated internalization of S. aureus. Targeting active PI-4,5-P-2 phosphatase to the plasma membrane and specific ablation of talin and FAK-binding motif in PIP5KI gamma 90 can reduce bacterial invasion.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biology
Ana Bura, Antonija Jurak Begonja
Summary: Phosphoinositides play crucial roles in cells with limited understanding of their localization and functions in platelets. This study investigated the localization of PI(4,5)P-2 and PI4P in resting and activated platelets through antibody staining. The intracellular pools of PI(4,5)P-2 and PI4P in platelets can be modulated by inhibitors of OCRL phosphatase and PI4KIII alpha kinase, with a more sensitive response in activated platelets.
Review
Cell Biology
Ana Bura, Sara Cabrijan, Iris Duric, Tea Bruketa, Antonija Jurak Begonja
Summary: Phosphoinositides (PIs) are phosphorylated lipids that have diverse cellular functions including regulation of trafficking, actin reorganization, and cell signaling. The two most abundant PIs in cells are phosphatidylinositol4-monophosphate (PI4P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], which localize to different cellular compartments and regulate various processes. The levels of PIs are controlled by multiple kinases and phosphatases, and understanding their localization and function is important for studying cellular processes.
Article
Cell Biology
Martin Sztacho, Barbora Salovska, Jakub Cervenka, Can Balaban, Peter Hoboth, Pavel Hozak
Summary: Analysis of nuclear protein interactions with phosphoinositides reveals the role of PIP2 in regulating gene expression, RNA splicing, and cell cycle processes. The identified proteins are involved in various functions within the nucleus, with different PIP2-binding motifs influencing their localization and activity. This study provides insights into the molecular mechanism of nuclear PIP2 protein interaction and offers a methodology for further research on PIPs and other protein ligands.
Article
Chemistry, Physical
Navjeet Ahalawat, Jagannath Mondal
Summary: Computer simulation approaches have greatly advanced in biomolecular recognition processes, particularly through unbiased simulation and Markov state modeling methods. Enhanced sampling approaches are crucial in accelerating simulations of ligand recognition processes, but key issues such as force fields and sampling bottlenecks still need to be addressed to achieve quantitative reconstruction of experimental measurements. Adopting multiscale approaches and coarse-grained simulations may be a possible way forward for efficient elucidation of ligand binding kinetics.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biology
Katerina Cizkova, Katerina Koubova, Zdenek Tauber
Summary: This study revealed that activators and inhibitors of PPAR alpha affect the PI3K/Akt/PTEN pathway in intestinal cell differentiation, highlighting their impact on brush border formation.
Article
Biochemistry & Molecular Biology
Li Zhang, Lei Li, Ziqing Wei, Hao Zhou, Haowen Liu, Shen Wang, Yijing Ren, Tiankai Dai, Jiafan Wang, Zhitao Hu, Cong Ma
Summary: This study reveals the crystal structure of the CAPS-1 C2PH module, showing a tight interaction between the C2 and PH domains and enhanced binding to PI(4,5)P2 membrane. A novel PI(4,5)P2-binding site is identified in the C2 domain. Disruption of the C2-PH interaction or the PIP2-binding sites impairs CAPS-1 function in Ca2+-regulated exocytosis.
Review
Biochemistry & Molecular Biology
Yi Wen, Volker M. Vogt, Gerald W. Feigenson
Summary: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a crucial component located at the inner leaflet of the cell membrane, constituting only 1-2% of total membrane lipids. Its synthesis and turnover are spatially and temporally regulated. Various factors, such as multivalent cations and cellular proteins interacting with PI(4,5)P-2, contribute to its versatile and dynamic distribution within membranes.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Article
Multidisciplinary Sciences
Veronika Thallmair, Lea Schultz, Wencai Zhao, Siewert J. Marrink, Dominik Oliver, Sebastian Thallmair
Summary: Phosphoinositides (PIs), especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2], play a crucial role in targeting proteins to cilia. This study discovered that the tubby domain, which is essential for this targeting process, binds to PI(4,5)P-2 through two binding sites.
Article
Physics, Multidisciplinary
Chenceline Fouedji, Armand Sylvin Eteme, Conrad Bertrand Tabi, Henri Paul Ekobena Fouda
Summary: Investigated the wave instability conditions in a reaction-diffusion system describing the dynamics of MARCKS between cytosol and cytoplasmic membrane, revealing different spatial patterns in the system.
EUROPEAN PHYSICAL JOURNAL PLUS
(2023)
Article
Physiology
Junlan Zhou, Neha Singh, Chloe Monnier, William Marszalec, Li Gao, Jing Jin, Michael Frisk, William E. Louch, Suresh Verma, Prasanna Krishnamurthy, Elsa Nico, Maaz Mulla, Gary L. Aistrup, Raj Kishore, J. Andrew Wasserstrom
Summary: The study demonstrated that PIP2-mediated targeting of BIN1 to the sarcolemma may be compromised during heart failure development, leading to T-tubule remodeling. Additionally, the interaction between PIP2 and cardiac BIN1 is essential for T-tubule maintenance and function, with depletion of PIP2 potentially causing T-tubule disruption.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maiwase Tembo, Rachel E. Bainbridge, Crystal Lara-Santos, Kayla M. Komondor, Grant J. Daskivich, Jacob D. Durrant, Joel C. Rosenbaum, Anne E. Carlson
Summary: This study investigates the interaction between TMEM16A channel and the membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2). The findings show that only lipids containing a phosphate at the 40 position effectively recover TMEM16A currents. These findings improve our understanding of how PI(4,5)P-2 binds to and potentiates TMEM16A channels.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marco van den Noort, Marijn de Boer, Bert Poolman
Summary: This study demonstrates that different ligands induce distinct bound conformations, and by changing the free energies of available conformations, ligand affinity can be adjusted. Experimental data on the maltose-binding protein (MBP) from Escherichia coli reveals that ligand affinity can be modulated through altering conformations.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Xiaofei Wang, Yawen Li, Hong Wei, Zhisen Yang, Rui Luo, Yongxiang Gao, Wei Zhang, Xin Liu, Linfeng Sun
Summary: TRPA1 is an ion channel that responds to noxious temperature or chemical stimuli. The thermosensitivity of TRPA1 varies among different species or isoforms. This study determines the structures of a heat-sensitive isoform of TRPA1 in fruit flies and identifies conformational changes in the ankyrin repeat domain and coiled-coil domain. The findings provide insights into the gating mechanism of TRPA1.
Article
Pharmacology & Pharmacy
Prakash Raut, Sasha R. R. Weller, Bright Obeng, Brandy L. L. Soos, Bailey E. E. West, Christian M. M. Potts, Suraj Sangroula, Marissa S. S. Kinney, John E. E. Burnell, Benjamin L. L. King, Julie A. A. Gosse, Samuel T. T. Hess
Summary: The potential therapeutic compound cetylpyridinium chloride (CPC) has shown promising effects in reducing mortality and viral load in influenza infection. CPC disrupts the density and clustering of influenza viral protein HA, inhibits the binding of HA with mammalian lipid PIP2, and displaces PIP2-binding protein MARCKS, leading to reduced influenza infectivity.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
M. B. Camarada, J. Comer, H. Poblete, E. R. Azhagiya Singam, V. Marquez-Miranda, C. Morales-Verdejo, F. D. Gonzalez-Nilo
Article
Chemistry, Multidisciplinary
Erik Jacques, Manuel Ahumada, Brianna Rector, Goonay Yousefalizadeh, Constanza Galaz-Araya, Rodrigo Recabarren, Kevin Stamplecoskie, Horacio Poblete, Emilio I. Alarcon
Article
Chemistry, Physical
E. R. Azhagiya Singam, Yuntao Zhang, Geraldine Magnin, Ingrid Miranda-Carvajal, Logan Coates, Ravindra Thakkar, Horacio Poblete, Jeffrey Comer
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2019)
Article
Nanoscience & Nanotechnology
Keshav Goel, Matias Zuniga-Bustos, Caitlin Lazurko, Erik Jacques, Constanza Galaz-Araya, Francisco Valenzuela-Henriquez, Natalia L. Pacioni, Jean-Francois Couture, Horacio Poblete, Emilio I. Alarcon
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Biophysics
Ariela Vergara-Jaque, Francisco Palma-Cerda, Adam S. Lowet, Angel de la Cruz Landrau, Horacio Poblete, Alexander Sukharev, Jeffrey Comer, Miguel Holmgren
BIOPHYSICAL JOURNAL
(2019)
Article
Biology
Hans Moldenhauer, Ignacio Diaz-Franulic, Horacio Poblete, David Naranjo
Article
Nanoscience & Nanotechnology
Christopher D. McTiernan, David C. Cortes, Caitlin Lazurko, Selya Amrani, Roberto Rosales-Rojas, Matias Zuniga-Bustos, Veronika Sedlakova, Horacio Poblete, Kevin Stamplecoskie, Erik J. Suuronen, Emilio I. Alarcon
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Biology
Wenchang Zhou, Giacomo Fiorin, Claudio Anselmi, Hossein Ali Karimi-Varzaneh, Horacio Poblete Section, Lucy R. Forrest, Jose D. Faraldo-Gomez
Article
Biochemistry & Molecular Biology
Jose Rivas, Nicolas Diaz, Ian Silva, Danna Morales, Boris Lavanderos, Alhejandra Alvarez, Maria Paz Saldias, Eduardo Pulgar, Pablo Cruz, Diego Maureira, Guillermo Flores, Alicia Colombo, Constanza Blanco, Hector R. Contreras, Fabian Jana, Ivan Gallegos, Miguel L. Concha, Ariela Vergara-Jaque, Horacio Poblete, Wendy Gonzalez, Diego Varela, James S. Trimmer, Monica Caceres, Oscar Cerda
Article
Chemistry, Applied
Daniel Zuniga-Nunez, Francisco Mura, Ricardo A. Zamora, Horacio Poblete, Denis Fuentealba, Alexis Aspee
Article
Chemistry, Multidisciplinary
Marcelo Munoz, Cagla Eren Cimenci, Keshav Goel, Maxime Comtois-Bona, Mahir Hossain, Christopher McTiernan, Matias Zuniga-Bustos, Alex Ross, Brenda Truong, Darryl R. Davis, Wenbin Liang, Benjamin Rotstein, Marc Ruel, Horacio Poblete, Erik J. Suuronen, Emilio Alarcon
Summary: This article reports on the development and testing of a sprayable nanotherapeutic that can improve cardiac function and conductivity, reduce infarct size, and increase the number of blood vessels and cardiomyocytes in infarcted myocardium.
Article
Pharmacology & Pharmacy
Roberto Rosales-Rojas, Matias Zuniga-Bustos, Francisca Salas-Sepulveda, Constanza Galaz-Araya, Ricardo A. Zamora, Horacio Poblete
Summary: This study focuses on the computational investigation of the structural micro-organization of hydrogels based on collagen-like peptides (CLPs) in complex with Rose Bengal (RB). The results suggest that RB can stabilize ordered three-dimensional structures and provide a basis for the development of improved synthetic peptides for photochemical tissue bonding therapies.
Article
Chemistry, Medicinal
Daniel Bustos, Erix W. Hernandez-Rodriguez, Horacio Poblete, Jans Alzate-Morales, Cecilia Challier, Cristhian Boetsch, Ariela Vergara-Jaque, Paola Beassoni
Summary: This study investigates the inhibitory site of PchP and describes a coupled inhibition mechanism by the substrate. The results contribute to a better understanding of PchP function and provide the basis for rational drug design to treat infections caused by Pseudomonas aeruginosa.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Chemistry, Multidisciplinary
Alpna Bisht, Matias Zuniga-Bustos, Garima Prasher, Surbhi Gautam, Horacio Poblete, Raman Preet Singh
Summary: Polyoxyethylene sorbitan monooleate is commonly used to stabilize dispersion of carbon nanoparticles such as carbon nanotubes and graphene. However, the mechanism of dispersion is not well understood. This study used spectroscopy and molecular dynamics simulations to investigate the mechanism of stabilization of carbon nanoparticles by Tween-80. The results showed that Tween-80 adsorbs onto the surface of carbon nanotubes and graphene through interaction between the alkyl chain and the aromatic rings, and the hydrophilic chains stabilize the suspension by steric hindrance. The simulations also revealed concentration-dependent changes in the nature of interactions between Tween-80 and the carbon nanoparticles, with aggregation shifting from planar to micelle-like structures.
Article
Chemistry, Physical
Christopher D. McTiernan, Matias Zuniga-Bustos, Roberto Rosales-Rojas, Pablo Barrias, May Griffith, Horacio Poblete, Peter S. Sherin, Ismael Lopez-Duarte, Marina K. Kuimova, Emilio Alarcon
Summary: Our study suggests that incorporating a positive charge on the hydrophobic tail of the BODIPY molecular rotor enhances its specificity as a reporter for viscosity of collagen-like peptide solutions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)