期刊
PROCESS BIOCHEMISTRY
卷 83, 期 -, 页码 183-188出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2019.05.008
关键词
Bacillus subtilis (natto) fermentation; Wet cell; Macroporous resin; Menaquinone-7; Purification
资金
- Major Projects of Science and Technology of Anhui Province [17030801036]
- Anhui Provincial Natural Science Foundation [1908085MB43]
Meanquinone-7 (MK-7) can be produced by the fermentation of Bacillus subtilis (natto), which is known for its nutraceutical and pharmacological properties. This study focused on the separation and purification of meanquinone-7 from wet cells produced by Bacillus subtilis (natto) fermentation. Using ethanol to extract menaquinone-7 twice directly from the crude cells after fermentation produced a menaquinone-7 yield of 1.47mg/g. The crude extract was purified using macroporous resin and subsequent crystallization. After treatment with HPD722 resin, the purification of menaquinone-7 were 7.17-fold increased with recovery yields of 97.2%. Menaquinone-7 with purities of more than 96% were produced with recovery yield of 99.3% by recrystallized with 95% ethanol. The developed procedure can produce high-purity of menaquinone-7 as well as high recoveries. This is an economical, efficient and simple process to large-scale preparation of high-purity menaquinone-7.
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