Article
Plant Sciences
Kunxin Wu, Yan Fu, Yanli Ren, Linyu Liu, Xiuchun Zhang, Mengbin Ruan
Summary: This study reveals that Turnip crinkle virus (TCV) activates mRNA decay pathways during infection, and the viral protein interferes with this pathway to facilitate viral replication.
Article
Plant Sciences
Md Emran Ali, Sumyya Waliullah
Summary: Contrary to popular belief, the 2X35S promoter actually delayed the initiation of turnip crinkle virus (TCV) infection compared to the Core35S promoter. This suggests that the Core35S (or mini35S) promoter may be a better choice for generating infectious cDNA clones of TCV, as the stronger 2X35S promoter may lead to higher accumulation of a TCV protein that inhibits virus replication.
Article
Virology
Shaoyan Zhang, Rong Sun, Camila Perdoncini Carvalho, Junping Han, Limin Zheng, Feng Qu
Summary: Viral long noncoding RNAs (lncRNAs) produced during cellular infections of positive-strand (+) RNA viruses can arise through different mechanisms, including replication-dependent and 5'-to-3' degradation stalled at stable structures. The viral lncRNA ttsgR produced by turnip crinkle virus (TCV) is regulated by RdRp, amplified on templates with CCS sequence with replication proteins, leading to the emergence of another lncRNA.
JOURNAL OF VIROLOGY
(2021)
Article
Plant Sciences
Linyu Liu, Haiyan Wang, Yan Fu, Wen Tang, Pingjuan Zhao, Yanli Ren, Zhixin Liu, Kunxin Wu, Xiuchun Zhang
Summary: The capsid protein of Turnip crinkle virus interacts with SGS3, enhancing viral accumulation and suppressing RNA silencing-mediated antiviral defense.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Natasha Marques Cassani, Igor Andrade Santos, Victoria Riquena Grosche, Giulia Magalhaes Ferreira, Marco Guevara-Vega, Rafael Borges Rosa, Lindomar Jose Pena, Nilson Nicolau-Junior, Adelia Cristina Oliveira Cintra, Tiago Patriarca Mineo, Robinson Sabino -Silva, Suely Vilela Sampaio, Ana Carolina Gomes Jardim
Summary: This study demonstrates that two phospholipases A2 (PLA2) isolated from snake venom can inhibit the activity of Zika virus, with a strong effect on the entry of the virus into host cells. These findings suggest that these PLA2s could serve as potential templates for the development of antiviral drugs against Zika virus in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Medicinal
Yejin Jang, Jinhe Han, Xiaoli Li, Hyunjin Shin, Won-Jea Cho, Meehyein Kim
Summary: Influenza viruses are major pathogens causing respiratory infections in humans. A newly discovered isoquinolone compound showed potential in inhibiting influenza A and B viruses, but exhibited cytotoxic effects. Through chemical optimization, compound 21 was developed with improved antiviral activity and reduced cytotoxicity.
Article
Chemistry, Multidisciplinary
Si Li, Xiao Guo, Rui Gao, Maozhong Sun, Liguang Xu, Chuanlai Xu, Hua Kuang
Summary: Rapid advances have been made in the application of nanomaterials to virus research, offering new approaches for virus detection, tracking, and antiviral strategies with promising prospects for further development.
ADVANCED MATERIALS
(2021)
Article
Agriculture, Dairy & Animal Science
Xiaoyu Men, Su Li, Xiaojing Cai, Lian Fu, Yi Shao, Yan Zhu
Summary: Pseudorabies virus (PRV) is a major threat to swine industry and can potentially cause viral encephalitis in humans. Due to emerging variants, finding effective antiviral agents against PRV is crucial. This study evaluated the antiviral activity of luteolin against PRV both in vitro and in vivo. Luteolin demonstrated significant inhibition of PRV replication, reduced viral RNA and protein expression, decreased apoptosis, improved survival rate in mice, and reduced viral loads and brain lesions. These findings suggest that luteolin holds promise as a new alternative antiviral drug for PRV infection.
Article
Veterinary Sciences
Lixia Li, Rui Wang, Huaiyue Hu, Xu Chen, Zhongqiong Yin, Xiaoxia Liang, Changliang He, Lizi Yin, Gang Ye, Yuanfeng Zou, Guizhou Yue, Huaqiao Tang, Renyong Jia, Xu Song
Summary: The results of this study indicate that kaempferol shows anti-Pseudorabies virus activity in mice, improving survival rates, inhibiting virus replication, alleviating pathogenic changes in organs, and affecting viral gene transcription.
BMC VETERINARY RESEARCH
(2021)
Article
Biochemical Research Methods
Kunxin Wu, Yadan Wu, Chunwei Zhang, Yan Fu, Zhixin Liu, Xiuchun Zhang
Summary: This study introduces a novel VIGS vector, CPB1B, for preliminary assessment of gene silencing penetrance in Arabidopsis, allowing simultaneous silencing of multiple genes. Additionally, the involvement of DCL4 and AGO2 in antiviral defense was confirmed through visual markers using this vector.
Article
Medicine, Research & Experimental
Won-Kyung Cho, Jin Yeul Ma
Summary: Epimedium koreanum Nakai (EKN) has shown significant inhibitory effects on influenza A virus infection by directly blocking viral attachment and having a virucidal effect. It can potentially be developed as a natural viral inhibitor against influenza virus infection.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Virology
Ching-Hsuan Liu, Yee-Tung Hu, Shu Hui Wong, Liang-Tzung Lin
Summary: Since the 2014-2016 epidemic, Ebola virus has become a major threat to global health. Despite the approval of several anti-Ebola vaccines and therapeutics, the high fatality rate highlights the need for continuous development of antiviral strategies.
Article
Horticulture
Yingjuan Huang, Ruicheng Liu, Ruiqi Song, Dong Zhou, Jingwen Chen, Rui Liu, Weiwei Chen, Jianli Yang, Xiaolin Yu, Jiashu Cao, Li Huang
Summary: This study investigated the molecular mechanism underlying the development of turnip hypocotyl-tuber. Comparative transcriptomic analysis revealed that changes in the expression patterns of genes related to plant hormones, especially auxin, were closely related to turnip hypocotyl enlargement. The localization of auxin during early hypocotyl development stages also exhibited differences between turnip and pak-choi subspecies. These findings contribute to a better understanding of auxin's role in turnip hypocotyl enlargement.
SCIENTIA HORTICULTURAE
(2023)
Review
Fisheries
Shuang-Shuang Sun, Shi-Wei Ma, Jun Li, Qin Zhang, Guang-Zhou Zhou
Summary: Fish rhabdoviruses are harmful viruses that cause significant economic losses in aquaculture. This review examines over 50 organic compounds that have shown effectiveness in preventing and treating rhabdovirus infection in fish. These compounds have various mechanisms of action and lay the foundation for future development of new strategies against fish rhabdoviruses.
Article
Microbiology
Yu-Yuan Zhang, Ming-Xia Sun, Yuexiao Lian, Tong-Yun Wang, Mei-Yu Jia, Chaoliang Leng, Meng Chen, Yuan-Zhe Bai, Fandan Meng, Xue-Hui Cai, Yan-Dong Tang
Summary: In recent years, Seneca Valley virus (SVV) has emerged as a newly identified pathogen of porcine vesicular disease, spreading quickly and posing a potential threat to the swine industry in multiple countries. Currently, there are no commercial vaccines or drugs available to combat SVV, highlighting the need for strategies to prevent and control SVV infection. A study found that the CRISPR-Cas13d system significantly inhibited SVV replication and demonstrated potent anti-SVV activity, offering a novel alternative strategy to control SVV epidemics in the future.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Jose C. Tovar, Carlos Quillatupa, Steven T. Callen, S. Elizabeth Castillo, Paige Pearson, Anastasia Shamin, Haley Schuhl, Noah Fahlgren, Malia A. Gehan
Article
Plant Sciences
Selena Rice, Emily Fryer, Suryatapa Ghosh Jha, Andrey Malkovskiy, Heather Meyer, Jason Thomas, Renee Weizbauer, Kangmei Zhao, Kenneth Birnbaum, David Ehrhardt, Zhiyong Wang, Seung Y. Rhee
Article
Multidisciplinary Sciences
Qi Li, Ningkun Liu, Qing Liu, Xingguo Zheng, Lu Lu, Wenrui Gao, Yang Liu, Yan Liu, Shicheng Zhang, Qian Wang, Jing Pan, Chen Chen, Yingjie Mi, Meiling Yang, Xiaofei Cheng, Guodong Ren, Yao-Wu Yuan, Xiaoming Zhang
Summary: The RNA helicases RH6, RH8, and RH12 play key roles in modulating the formation of membraneless nuclear dicing bodies (D-bodies) in Arabidopsis via liquid-liquid phase separations (LLPSs). Their accumulation decreases in the nuclei upon Turnip mosaic virus infections, leading to a decrease in D-bodies.
Article
Biology
Suryatapa Ghosh Jha, Alexander T. Borowsky, Benjamin J. Cole, Noah Fahlgren, Andrew Farmer, Shao-Shan Carol Huang, Purva Karia, Marc Libault, Nicholas J. Provart, Selena L. Rice, Maite Saura-Sanchez, Pinky Agarwal, Amir H. Ahkami, Christopher R. Anderton, Steven P. Briggs, Jennifer An Brophy, Peter Denolf, Luigi F. Di Costanzo, Moises Exposito-Alonso, Stefania Giacomello, Fabio Gomez-Cano, Kerstin Kaufmann, Dae Kwani Ko, Sagar Kumar, Andrey Malkovskiy, Naomi Nakayama, Toshihiro Obata, Marisa S. Otegui, Gergo Palfalvi, Elsa H. Quezada-Rodriguez, Rajveer Singh, R. Glen Uhrig, Jamie Waese, Klaas Van Wijk, R. Clay Wright, David W. Ehrhardt, Kenneth D. Birnbaum, Seung Y. Rhee
Summary: With growing populations and urgent environmental issues, the future economy will be increasingly plant-based. Reimagining plant science as a critical component across various fields is crucial. The concept of a Plant Cell Atlas (PCA) is proposed to better understand and engineer plant development, physiology, and environmental responses. A workshop was held to discuss the purpose and utility of this initiative, highlighting current knowledge gaps and technical challenges.
Article
Agricultural Engineering
Zinan Luo Lily, Noah Fahlgren, Toni Kutchan, Daniel Schachtman, Yufeng Ge, Russ Gesch, Sheeja George, John Dyer, Hussein Abdel-Haleem
Summary: Camelina sativa is a promising oilseed and industrial crop that benefits sustainable food, feed, and fuel industries. Early flowering is critical for local adaptation and maximizing yield in Camelina sativa. The study identified significant trait-associated markers through GWAS and demonstrated the potential for genetic improvements in flower development and timing in camelina.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Ben N. Mansfeld, Adam Boyher, Jeffrey C. Berry, Mark Wilson, Shujun Ou, Seth Polydore, Todd P. Michael, Noah Fahlgren, Rebecca S. Bart
Summary: This study utilized a combination of high-throughput sequencing technologies to resolve the highly heterozygous genome of cassava, revealing a large number of haplotype-specific structural variations that impact important agronomic traits, as well as exploring gene hemizygosity and allele-specific gene expression.
Article
Plant Sciences
Jose C. Tovar, Jeffrey C. Berry, Carlos Quillatupa, S. Elizabeth Castillo, Lucia Acosta-Gamboa, Noah Fahlgren, Malia A. Gehan
Summary: This study investigated the effects of heat stress in the roots or shoots of quinoa plants on the elemental concentrations in quinoa seed. The results showed that heat treatment significantly changed the elemental concentrations in quinoa seed, and the type of panicle and heat treatment method also had an impact. Additionally, the position of the plant section and the timing of heat exposure had significant effects on the seed elemental concentrations.
Article
Plant Sciences
Selena L. Rice, Elena Lazarus, Christopher Anderton, Kenneth Birnbaum, Jennifer Brophy, Benjamin Cole, Diane Dickel, David Ehrhardt, Noah Fahlgren, Margaret Frank, Elizabeth Haswell, Shao-shan Carol Huang, Samuel Leiboff, Marc Libault, Marisa S. Otegui, Nicholas Provart, R. Glen Uhrig, Seung Y. Rhee
Summary: This article discusses the virtual symposium hosted by the Plant Cell Atlas (PCA) community on single cell and spatial omics technologies, and explores the potential impact of establishing a data synthesis center. The discussions focused on topics such as data analysis tools, annotation standards, computational expertise, community organization and engagement, talent recruitment and training, and data visualization and sharing. The development of a data synthesis center will provide a centralized repository, platform for sharing tools, and collaborative analytical approaches to accelerate plant research.
Article
Plant Sciences
Noah Fahlgren, Muskan Kapoor, Galabina Yordanova, Irene Papatheodorou, Jamie Waese, Benjamin Cole, Peter Harrison, Doreen Ware, Timothy Tickle, Benedict Paten, Tony Burdett, Christine G. Elsik, Christopher K. Tuggle, Nicholas J. Provart
Summary: Building a data infrastructure for the Plant Cell Atlas using existing infrastructure and platforms will allow biologists and data scientists to gain new insights into plant biology. The development of the Human Cell Atlas and the Single Cell Expression Atlas by the European Bioinformatics Institute has laid the foundation for constructing the data infrastructure for the Plant Cell Atlas. The utilization of appropriate ontologies is crucial for describing plant single cell experiments.
Article
Plant Sciences
Kaushik Panda, Boominathan Mohanasundaram, Jorge Gutierrez, Lauren McLain, S. Elizabeth Castillo, Hudanyun Sheng, Anna Casto, Gustavo Gratacos, Ayan Chakrabarti, Noah Fahlgren, Sona Pandey, Malia A. A. Gehan, R. Keith Slotkin
Summary: Plant responses to abiotic environmental challenges can have lasting effects on the next generation. The response of plants to high CO2 levels has been well studied, but transgenerational studies are rare.
Article
Plant Sciences
Ella Ludwig, Joshua Sumner, Jeffrey Berry, Seth Polydore, Tracy Ficor, Erica Agnew, Kristina Haines, Kathleen Greenham, Noah Fahlgren, Todd C. Mockler, Malia A. Gehan
Summary: The study examines the variation in phenotypes of 149 accessions of Brachypodium distachyon under drought, heat, and combined stresses. Heat causes the most tissue damage, while the combination of stresses leads to the greatest biomass decrease. The climate of origin is significantly associated with the plant's response to stress conditions.
Meeting Abstract
Biochemistry & Molecular Biology
D. Lin, R. Bart, J. C. Carrington
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Proceedings Paper
Computer Science, Artificial Intelligence
David LeBauer, Max Burnette, Noah Fahlgren, Rob Kooper, Kenton McHenry, Abby Stylianou
Summary: The TERRA-REF project aimed to create an open-access reference dataset for plant research by deploying high-resolution sensors and collecting traditional plant phenotype measurements. This unique data resource, consisting of cutting-edge technology sensors and various plant-related data, provides unprecedented opportunities for investigations in the Computer Vision and Machine Learning communities.
2021 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW 2021)
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
D. Lin, R. Bart, J. C. Carrington
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Meeting Abstract
Plant Sciences
Z. J. Lin, E. De Meyer, H. Tiley, R. Bart, J. C. Carrington